################################## # NO CUT ENZYMES ( 169 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflIII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BcgI BciVI BcnI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KpnI KspI MluI MluNI MroI MscI MslI MspI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tsp45I Tth111I TthHB8I Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AflII 373 CTTAAG C^TTAAG Alw44I 860 GTGCAC G^TGCAC AlwNI 1028 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 860 GTGCAC G^TGCAC AspHI 860 GTGCAC G[AT]GC[AT]^C BanII 217 GAGCCC G[GA]GC[CT]^C BbvCI 331 GCTGAGG CCTCAGC(-5/-2) BfrI 373 CTTAAG C^TTAAG BpmI 614 CTGGAG CTGGAG(16/14) BsaBI 337 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiHKAI 860 GTGCAC G[AT]GC[AT]^C BsmFI 1072 GTCCC GGGAC(10/14) BstAPI 322 GCAATAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 540 GGATCT [GA]^GATC[CT] DraI 949 TTTAAA TTT^AAA DraII 229 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoO109I 229 GGGGCCT [GA]G^G[ACGT]CC[CT] HincII 531 GTCAAC GT[CT]^[GA]AC HindII 531 GTCAAC GT[CT]^[GA]AC MaeII 249 ACGT A^CGT MamI 337 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1010 CAATTG C^AATTG MflI 540 GGATCT [GA]^GATC[CT] MunI 1010 CAATTG C^AATTG PstI 447 CTGCAG CTGCA^G ScaI 780 AGTACT AGT^ACT SmlI 373 CTTAAG C^T[CT][GA]AG XbaI 367 TCTAGA T^CTAGA XhoII 540 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AlwI 540 GGATC GGATC(4/5) 962 GGATC BbsI 436 GAAGAC GAAGAC(2/6) 279 GTCTTC BclI 207 TGATCA T^GATCA 510 TGATCA BmyI 217 GAGCCC G[AGT]GC[ACT]^C 860 GTGCAC BpuAI 436 GAAGAC GAAGAC(2/6) 279 GTCTTC BsmI 1203 GAATGC GAATGC(1/-1) 657 GCATTC Bsp1286I 217 GAGCCC G[AGT]GC[ACT]^C 860 GTGCAC BspMI 318 ACCTGC ACCTGC(4/8) 804 ACCTGC BsrI 778 CCAGT ACTGG(1/-1) 839 CCAGT FbaI 207 TGATCA T^GATCA 510 TGATCA HphI 307 GGTGA GGTGA(8/7) 571 GGTGA MspA1I 1094 CAGCTG C[AC]G^C[GT]G 1228 CAGCTG NlaIII 25 CATG CATG^ 99 CATG NlaIV 229 GGGGCC GG[ACGT]^[ACGT]CC 1005 GGAGCC PleI 439 GACTC GAGTC(4/5) 277 GAGTC PvuII 1094 CAGCTG CAG^CTG 1228 CAGCTG SfcI 447 CTGCAG C^T[GA][CT]AG 1276 CTACAG ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AcsI AfaI AluI ApoI BbvI BfaI BsaJI BsiYI BslI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HinfI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII RsaI Sau3AI Sau96I ScrFI SfaNI TfiI Tru9I TseI Tsp509I TspRI