################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI Eco47III Eco81I EcoO109I EcoO65I EcoRV EcoT22I FbaI FokI FseI FspI HgaI HincII HindII HindIII HpaI KpnI MaeI MamI MfeI MluI MluNI MroI MscI MslI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SalI SapI SbfI ScaI SexAI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tth111I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AcyI 310 GGCGCC G[GA]^CG[CT]C AhdI 841 GACTTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 204 GATCC GGATC(4/5) AspEI 841 GACTTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 744 CCCGGG C^[CT]CG[GA]G BanI 310 GGCGCC G^G[CT][GA]CC BbeI 310 GGCGCC GGCGC^C BglI 541 GCCGCGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 274 TACGTG [CT]AC^GT[GA] BsaHI 310 GGCGCC G[GA]^CG[CT]C BsaWI 521 ACCGGA [AT]^CCGG[AT] BsgI 127 CTGCAC GTGCAG(16/14) BsiEI 297 CGGCCG CG[GA][CT]^CG BsmFI 365 GGGAC GGGAC(10/14) BsoBI 744 CCCGGG C^[CT]CG[GA]G BsrBI 631 CCGCTC CCGCTC(-3/-3) BsrFI 481 ACCGGC [GA]^CCGG[CT] BstYI 203 AGATCC [GA]^GATC[CT] Cfr10I 481 ACCGGC [GA]^CCGG[CT] DdeI 645 CTCAG C^T[ACGT]AG DpnII 204 GATC ^GATC EagI 297 CGGCCG C^GGCCG Eam1105I 841 GACTTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 649 GAAGAG CTCTTC(1/4) EclXI 297 CGGCCG C^GGCCG Eco52I 297 CGGCCG C^GGCCG EcoNI 613 CCTCTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 655 GAATTC G^AATTC Hin1I 310 GGCGCC G[GA]^CG[CT]C HinfI 805 GACTC G^A[ACGT]TC KasI 310 GGCGCC G^GCGCC Ksp632I 649 GAAGAG CTCTTC(1/4) MaeII 275 ACGT A^CGT MboI 204 GATC ^GATC MboII 649 GAAGA GAAGA(8/7) MflI 203 AGATCC [GA]^GATC[CT] NarI 310 GGCGCC GG^CGCC NdeII 204 GATC ^GATC NlaIII 636 CATG CATG^ PleI 805 GACTC GAGTC(4/5) Sau3AI 204 GATC ^GATC SfiI 540 GGCCGCGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 310 GGCGCC GGC^GCC SmaI 744 CCCGGG CCC^GGG SmlI 148 CTCAAG C^T[CT][GA]AG XhoII 203 AGATCC [GA]^GATC[CT] XmaCI 744 CCCGGG C^CCGGG XmaI 744 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AcsI 340 AAATTT [GA]^AATT[CT] 655 GAATTC AfaI 145 GTAC GT^AC 426 GTAC ApoI 340 AAATTT [GA]^AATT[CT] 655 GAATTC AspHI 193 GAGCTC G[AT]GC[AT]^C 726 GAGCTC AvaII 690 GGTCC G^G[AT]CC 848 GGTCC BanII 193 GAGCTC G[GA]GC[CT]^C 726 GAGCTC BmyI 193 GAGCTC G[AGT]GC[ACT]^C 726 GAGCTC BsaI 376 GAGACC GGTCTC(1/5) 533 GAGACC BsiHKAI 193 GAGCTC G[AT]GC[AT]^C 726 GAGCTC BsmAI 376 GAGAC GTCTC(1/5) 533 GAGAC Bsp1286I 193 GAGCTC G[AGT]GC[ACT]^C 726 GAGCTC BspMI 125 ACCTGC ACCTGC(4/8) 92 GCAGGT BstXI 155 CCAAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 830 CCAGTCTGGTGG EaeI 297 CGGCCG [CT]^GGCC[GA] 470 TGGCCG EcoT14I 155 CCAAGG C^C[AT][AT]GG 551 CCAAGG HaeII 83 AGCGCC [GA]GCGC^[CT] 310 GGCGCC KspI 177 CCGCGG CCGC^GG 542 CCGCGG MaeIII 105 GTCAC ^GT[ACGT]AC 770 GTCAC MseI 515 TTAA T^TAA 751 TTAA MspA1I 177 CCGCGG C[AC]G^C[GT]G 542 CCGCGG RsaI 145 GTAC GT^AC 426 GTAC SacI 193 GAGCTC GAGCT^C 726 GAGCTC SacII 177 CCGCGG CCGC^GG 542 CCGCGG SfaNI 6 GATGC GCATC(5/9) 792 GCATC StyI 155 CCAAGG C^C[AT][AT]GG 551 CCAAGG TaqI 4 TCGA T^CGA 403 TCGA Tru9I 515 TTAA T^TAA 751 TTAA Tsp45I 105 GTCAC ^GT[GC]AC 770 GTCAC Tsp509I 341 AATT ^AATT 656 AATT TspRI 107 CACTG CA[GC]TG[ACGT][ACGT]^ 347 CACTG TthHB8I 4 TCGA T^CGA 403 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AccII AciI AluI AlwNI BbvI BcnI BpmI BsaJI BseRI BsiYI BslI BsrI BssKI BstNI BstUI Cac8I CfoI Cfr13I DsaI EcoRII FauI Fnu4HI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MnlI MspI MvaI MvnI MwoI NciI NlaIV PstI Sau96I ScrFI SfcI TseI