(Circular) (Six-base) MAPSORT of: pFN21AB8933.seq Check: 6554 from: 1 to: 5557 pFN21AB8933 5557 bp With 182 enzymes: SgfI * March 28, 2012 14:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2139 2139 Size: 5557 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3367 278 Size: 53 83 186 936 1831 2468 Fragments arranged by size: 2468 1831 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2900 1937 Size: 963 4594 Acc65I G'GTAC_C Cuts at: 2878 2878 Size: 5557 AclI AA'CG_TT Cuts at: 1563 3684 4057 1563 Size: 2121 373 3063 Fragments arranged by size: 3063 2121 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2288 3620 5004 5272 1632 Size: 656 1332 1384 268 1917 Fragments arranged by size: 1917 1384 1332 656 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5360 AflIII A'CryG_T Cuts at: 1203 4456 5433 1203 Size: 3253 977 1327 Fragments arranged by size: 3253 1327 977 AgeI A'CCGG_T Cuts at: 4388 4388 Size: 5557 AhdI GACnn_n'nnGTC Cuts at: 2851 4286 2851 Size: 1435 4122 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5557 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5557 AlwNI CAG_nnn'CTG Cuts at: 2260 2391 2514 3300 4872 2260 Size: 131 123 786 1572 2945 Fragments arranged by size: 2945 1572 786 131 123 ApaLI G'TGCA_C Cuts at: 3614 4770 3614 Size: 1156 4401 ApoI r'AATT_y Cuts at: 1493 2345 2660 2866 3092 3244 5353 1493 Size: 852 315 206 226 152 2109 1697 Fragments arranged by size: 2109 1697 852 315 226 206 152 AseI AT'TA_AT Cuts at: 160 1032 4111 160 Size: 872 3079 1606 Fragments arranged by size: 3079 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5557 AvaI C'yCGr_G Cuts at: 1870 1943 2749 2882 1870 Size: 73 806 133 4545 Fragments arranged by size: 4545 806 133 73 BamHI G'GATC_C Cuts at: 2887 3254 2887 Size: 367 5190 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2315 2878 4333 618 Size: 325 217 627 528 563 1455 1842 Fragments arranged by size: 1842 1455 627 563 528 325 217 BanII G_rGCy'C Cuts at: 1391 2202 2735 2876 1391 Size: 811 533 141 4072 Fragments arranged by size: 4072 811 533 141 BbeI G_GCGC'C Cuts at: 2319 2319 Size: 5557 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4586 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3748 3782 1903 Size: 34 1811 34 3678 Fragments arranged by size: 3678 1811 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3449 4659 3449 Size: 1210 4347 BclI T'GATC_A Cuts at: 1548 1548 Size: 5557 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2552 4168 136 Size: 107 122 71 631 605 880 1616 1525 Fragments arranged by size: 1616 1525 880 631 605 122 107 71 BglII A'GATC_T Cuts at: 5552 5552 Size: 5557 BlpI GC'TnA_GC Cuts at: 2974 2974 Size: 5557 Bme1580I G_kGCm'C Cuts at: 1790 3618 4774 1790 Size: 1828 1156 2573 Fragments arranged by size: 2573 1828 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5557 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2766 3288 4246 Size: 416 53 83 399 24 1349 522 958 Cuts at: 4246 442 Size: 1753 Fragments arranged by size: 1753 1349 958 522 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5557 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2680 2680 Size: 5557 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2105 2234 2818 4217 1239 Size: 866 129 584 1399 2579 Fragments arranged by size: 2579 1399 866 584 129 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2138 3682 4547 4845 2138 Size: 1544 865 298 2850 Fragments arranged by size: 2850 1544 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2375 2532 4220 882 Size: 617 876 157 1688 2219 Fragments arranged by size: 2219 1688 876 617 157 BsaAI yAC'GTr Cuts at: 493 1204 2281 3348 493 Size: 711 1077 1067 2702 Fragments arranged by size: 2702 1077 1067 711 BsaBI GATnn'nnATC Cuts at: 5551 5551 Size: 5557 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2316 3364 3746 4381 Size: 53 83 186 936 783 1048 382 635 Cuts at: 4381 275 Size: 1451 Fragments arranged by size: 1451 1048 936 783 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2526 3990 4388 4662 4809 5220 2526 Size: 1464 398 274 147 411 2863 Fragments arranged by size: 2863 1464 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2570 2576 2600 2606 1178 Size: 30 165 30 1167 6 24 6 4129 Fragments arranged by size: 4129 1167 165 30 30 24 6 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2393 2578 2581 2607 1186 Size: 1207 185 3 26 4136 Fragments arranged by size: 4136 1207 185 26 3 BseYI C'CCAG_C Cuts at: 4760 5256 4760 Size: 496 5061 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2117 1163 Size: 573 381 4603 Fragments arranged by size: 4603 573 381 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2305 3007 3768 4796 1540 Size: 275 186 304 702 761 1028 2301 Fragments arranged by size: 2301 1028 761 702 304 275 186 BsiHKAI G_wGCw'C Cuts at: 2202 2735 3618 3703 4774 2202 Size: 533 883 85 1071 2985 Fragments arranged by size: 2985 1071 883 533 85 BsmI GAATG_Cn' Cuts at: 3068 3161 3068 Size: 93 5464 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2202 2735 2876 3618 3703 4774 1391 Size: 399 412 533 141 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 533 412 399 141 85 BspEI T'CCGG_A Cuts at: 5220 5220 Size: 5557 BspHI T'CATG_A Cuts at: 3446 5176 3446 Size: 1730 3827 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2088 2139 2912 844 Size: 1244 51 773 3489 Fragments arranged by size: 3489 1244 773 51 BsrBI CCG'CTC Cuts at: 1394 1823 2638 3444 1394 Size: 429 815 806 3507 Fragments arranged by size: 3507 815 806 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4052 4226 65 Size: 1183 2804 174 1396 Fragments arranged by size: 2804 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2486 4201 4388 1700 Size: 110 676 1715 187 2869 Fragments arranged by size: 2869 1715 676 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5557 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5557 BssSI C'ACGA_G Cuts at: 3617 4629 3617 Size: 1012 4545 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5557 BstBI TT'CG_AA Cuts at: 4361 4361 Size: 5557 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5557 BstXI CCAn_nnnn'nTGG Cuts at: 2167 2842 2167 Size: 675 4882 BstYI r'GATC_y Cuts at: 1972 2208 2887 3254 3639 3656 5108 5194 5206 Size: 236 679 367 385 17 1452 86 12 Cuts at: 5206 5552 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 679 385 367 346 236 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2182 2319 2547 513 Size: 552 1117 137 228 3523 Fragments arranged by size: 3523 1117 552 228 137 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2436 487 Size: 683 56 1210 3608 Fragments arranged by size: 3608 1210 683 56 BtsI GCAGTG_nn' Cuts at: 2111 2351 3073 3867 3887 2111 Size: 240 722 794 20 3781 Fragments arranged by size: 3781 794 722 240 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5557 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5522 DraI TTT'AAA Cuts at: 2861 2861 Size: 5557 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4564 809 Size: 507 3248 1802 Fragments arranged by size: 3248 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2302 2475 3004 Size: 54 1425 50 275 18 472 173 529 Cuts at: 3004 3893 5522 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 529 472 275 173 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2302 3004 1537 Size: 275 490 702 4090 Fragments arranged by size: 4090 702 490 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2649 3487 1353 Size: 1296 838 3423 Fragments arranged by size: 3423 1296 838 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2091 4144 4518 4664 387 Size: 1064 138 502 2053 374 146 1280 Fragments arranged by size: 2053 1280 1064 502 374 146 138 EcoICRI GAG'CTC Cuts at: 2200 2733 2200 Size: 533 5024 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2105 2234 2288 2818 3620 4217 5004 Size: 393 473 129 54 530 802 597 787 Cuts at: 5004 5272 1239 Size: 268 1524 Fragments arranged by size: 1524 802 787 597 530 473 393 268 129 54 EcoNI CCTnn'n_nnAGG Cuts at: 2622 2622 Size: 5557 EcoRI G'AATT_C Cuts at: 2660 2866 3244 2660 Size: 206 378 4973 Fragments arranged by size: 4973 378 206 FspI TGC'GCA Cuts at: 4063 4063 Size: 5557 HaeII r_GCGC'y Cuts at: 1678 2092 2319 4704 1678 Size: 414 227 2385 2531 Fragments arranged by size: 2531 2385 414 227 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2675 2707 2837 2869 4198 Size: 32 135 32 1103 32 130 32 1329 Cuts at: 4198 4230 4272 4304 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1329 1103 135 130 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2901 3147 669 Size: 988 281 963 246 3079 Fragments arranged by size: 3079 988 963 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5557 HpaI GTT'AAC Cuts at: 3147 3147 Size: 5557 KasI G'GCGC_C Cuts at: 2315 2315 Size: 5557 KpnI G_GTAC'C Cuts at: 2882 2882 Size: 5557 MfeI C'AATT_G Cuts at: 3156 3156 Size: 5557 MluI A'CGCG_T Cuts at: 5433 5433 Size: 5557 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2619 4671 4855 1305 Size: 1314 2052 184 2007 Fragments arranged by size: 2052 2007 1314 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3735 Fragments arranged by size: 3735 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3515 3874 4033 518 Size: 648 843 1506 359 159 2042 Fragments arranged by size: 2042 1506 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2184 2549 2973 3650 4798 5043 1606 Size: 578 365 424 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 578 424 365 245 NarI GG'CG_CC Cuts at: 2316 2316 Size: 5557 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5005 NdeI CA'TA_TG Cuts at: 387 387 Size: 5557 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5557 NotI GC'GGCC_GC Cuts at: 3004 3004 Size: 5557 NspI r_CATG'y Cuts at: 1132 1229 2915 4460 1132 Size: 97 1686 1545 2229 Fragments arranged by size: 2229 1686 1545 97 PasI CC'CwG_GG Cuts at: 1348 2311 1348 Size: 963 4594 PciI A'CATG_T Cuts at: 4456 4456 Size: 5557 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5557 PfoI T'CCnGG_A Cuts at: 2211 2211 Size: 5557 PmeI GTTT'AAAC Cuts at: 2861 2861 Size: 5557 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2597 2629 3626 3658 5139 5171 2597 Size: 32 997 32 1481 32 2983 Fragments arranged by size: 2983 1481 997 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5557 PsiI TTA'TAA Cuts at: 3127 3127 Size: 5557 PstI C_TGCA'G Cuts at: 830 2070 2256 2709 2787 2909 830 Size: 1240 186 453 78 122 3478 Fragments arranged by size: 3478 1240 453 186 122 78 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5557 SacI G_AGCT'C Cuts at: 2202 2735 2202 Size: 533 5024 SacII CC_GC'GG Cuts at: 2185 2550 2185 Size: 365 5192 SalI G'TCGA_C Cuts at: 1936 2899 1936 Size: 963 4594 SbfI CC_TGCA'GG Cuts at: 2909 2909 Size: 5557 ScaI AGT'ACT Cuts at: 3805 5517 3805 Size: 1712 3845 SfcI C'TryA_G Cuts at: 826 1045 2066 2252 2705 2783 2905 4040 4721 Size: 219 1021 186 453 78 122 1135 681 Cuts at: 4721 4912 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1021 681 453 219 191 186 122 78 SfiI GGCCn_nnn'nGGCC Cuts at: 2552 2552 Size: 5557 SfoI GGC'GCC Cuts at: 2317 2317 Size: 5557 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5557 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5557 SmaI CCC'GGG Cuts at: 1872 2751 2884 1872 Size: 879 133 4545 Fragments arranged by size: 4545 879 133 SmlI C'TyrA_G Cuts at: 820 1017 1943 2153 3661 4562 4824 820 Size: 197 926 210 1508 901 262 1553 Fragments arranged by size: 1553 1508 926 901 262 210 197 SnaBI TAC'GTA Cuts at: 493 3348 493 Size: 2855 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5557 SphI G_CATG'C Cuts at: 1132 2915 1132 Size: 1783 3774 SspI AAT'ATT Cuts at: 5 52 3481 5 Size: 47 3429 2081 Fragments arranged by size: 3429 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5557 StyI C'CwwG_G Cuts at: 513 1065 2160 2556 2996 513 Size: 552 1095 396 440 3074 Fragments arranged by size: 3074 1095 552 440 396 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2516 2683 3584 3601 3754 3939 4384 Size: 1255 936 167 901 17 153 185 445 Cuts at: 4384 325 Size: 1498 Fragments arranged by size: 1498 1255 936 901 445 185 167 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3803 5515 96 Size: 274 80 33 51 1443 1826 1712 138 Fragments arranged by size: 1826 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5517 5549 5517 Size: 32 5525 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5557 XbaI T'CTAG_A Cuts at: 2893 2893 Size: 5557 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5557 XmaI C'CCGG_G Cuts at: 1870 2749 2882 1870 Size: 879 133 4545 Fragments arranged by size: 4545 879 133 XmnI GAAnn'nnTTC Cuts at: 3686 3686 Size: 5557 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3365 276 Size: 53 83 186 936 1831 2468 Fragments arranged by size: 2468 1831 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SanDI SapI SexAI SrfI SwaI XcmI