(Circular) (Six-base) MAPSORT of: pFN21AE0912.seq Check: 5861 from: 1 to: 5443 pFN21AE0912 5443 bp With 182 enzymes: SgfI * March 28, 2012 12:15 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2506 2506 Size: 5443 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3253 278 Size: 53 83 186 936 1717 2468 Fragments arranged by size: 2468 1717 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2786 1937 Size: 849 4594 Acc65I G'GTAC_C Cuts at: 2442 2764 2442 Size: 322 5121 AclI AA'CG_TT Cuts at: 1563 3570 3943 1563 Size: 2007 373 3063 Fragments arranged by size: 3063 2007 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2685 3506 4890 5158 1632 Size: 1053 821 1384 268 1917 Fragments arranged by size: 1917 1384 1053 821 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5246 AflIII A'CryG_T Cuts at: 1203 4342 5319 1203 Size: 3139 977 1327 Fragments arranged by size: 3139 1327 977 AgeI A'CCGG_T Cuts at: 4274 4274 Size: 5443 AhdI GACnn_n'nnGTC Cuts at: 4172 4172 Size: 5443 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5443 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5443 AlwNI CAG_nnn'CTG Cuts at: 2272 2593 3186 4758 2272 Size: 321 593 1572 2957 Fragments arranged by size: 2957 1572 593 321 ApaLI G'TGCA_C Cuts at: 3500 4656 3500 Size: 1156 4287 ApoI r'AATT_y Cuts at: 1493 2026 2752 2978 3130 5239 1493 Size: 533 726 226 152 2109 1697 Fragments arranged by size: 2109 1697 726 533 226 152 AseI AT'TA_AT Cuts at: 160 1032 3997 160 Size: 872 2965 1606 Fragments arranged by size: 2965 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5443 AvaI C'yCGr_G Cuts at: 1870 1943 2768 1870 Size: 73 825 4545 Fragments arranged by size: 4545 825 73 BamHI G'GATC_C Cuts at: 2452 2773 3140 2452 Size: 321 367 4755 Fragments arranged by size: 4755 367 321 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2442 2764 4219 618 Size: 325 217 627 655 322 1455 1842 Fragments arranged by size: 1842 1455 655 627 325 322 217 BanII G_rGCy'C Cuts at: 1391 2214 2487 2762 1391 Size: 823 273 275 4072 Fragments arranged by size: 4072 823 275 273 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2323 2402 928 Size: 971 424 79 3969 Fragments arranged by size: 3969 971 424 79 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3634 3668 1903 Size: 34 1697 34 3678 Fragments arranged by size: 3678 1697 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3335 4545 3335 Size: 1210 4233 BclI T'GATC_A Cuts at: 1548 1548 Size: 5443 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2129 2138 2183 Size: 107 122 71 631 605 457 9 45 Cuts at: 2183 4054 136 Size: 1871 1525 Fragments arranged by size: 1871 1525 631 605 457 122 107 71 45 9 BglII A'GATC_T Cuts at: 5438 5438 Size: 5443 BlpI GC'TnA_GC Cuts at: 2860 2860 Size: 5443 Bme1580I G_kGCm'C Cuts at: 1790 2617 3504 4660 1790 Size: 827 887 1156 2573 Fragments arranged by size: 2573 1156 887 827 BmgBI CAC'GTC Cuts at: 1318 2718 1318 Size: 1400 4043 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3174 4132 442 Size: 416 53 83 399 24 1757 958 1753 Fragments arranged by size: 1757 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5443 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2564 4103 1239 Size: 1325 1539 2579 Fragments arranged by size: 2579 1539 1325 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3568 4433 4731 3568 Size: 865 298 4280 Fragments arranged by size: 4280 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2054 4106 882 Size: 617 555 2052 2219 Fragments arranged by size: 2219 2052 617 555 BsaAI yAC'GTr Cuts at: 493 1204 3234 493 Size: 711 2030 2702 Fragments arranged by size: 2702 2030 711 BsaBI GATnn'nnATC Cuts at: 5437 5437 Size: 5443 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2655 3250 3632 4267 Size: 53 83 186 936 1122 595 382 635 Cuts at: 4267 275 Size: 1451 Fragments arranged by size: 1451 1122 936 635 595 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2498 3876 4274 4548 4695 5106 2498 Size: 1378 398 274 147 411 2835 Fragments arranged by size: 2835 1378 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5218 Fragments arranged by size: 5218 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5443 BseYI C'CCAG_C Cuts at: 2037 2468 2650 4646 5142 2037 Size: 431 182 1996 496 2338 Fragments arranged by size: 2338 1996 496 431 182 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4870 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2673 2893 3654 4682 1540 Size: 275 186 672 220 761 1028 2301 Fragments arranged by size: 2301 1028 761 672 275 220 186 BsiHKAI G_wGCw'C Cuts at: 2214 2487 3504 3589 4660 2214 Size: 273 1017 85 1071 2997 Fragments arranged by size: 2997 1071 1017 273 85 BsmI GAATG_Cn' Cuts at: 2954 3047 2954 Size: 93 5350 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2214 2487 2617 2762 3504 3589 4660 Size: 399 424 273 130 145 742 85 1071 Cuts at: 4660 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 424 399 273 145 130 85 BspEI T'CCGG_A Cuts at: 5106 5106 Size: 5443 BspHI T'CATG_A Cuts at: 3332 5062 3332 Size: 1730 3713 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2436 2454 2506 2578 2798 844 Size: 1592 18 52 72 220 3489 Fragments arranged by size: 3489 1592 220 72 52 18 BsrBI CCG'CTC Cuts at: 1394 1823 2076 2558 3330 1394 Size: 429 253 482 772 3507 Fragments arranged by size: 3507 772 482 429 253 BsrDI GCAATG_nn' Cuts at: 65 1248 3938 4112 65 Size: 1183 2690 174 1396 Fragments arranged by size: 2690 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4087 4274 1700 Size: 110 2277 187 2869 Fragments arranged by size: 2869 2277 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5443 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5443 BssSI C'ACGA_G Cuts at: 2172 3503 4515 2172 Size: 1331 1012 3100 Fragments arranged by size: 3100 1331 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5443 BstBI TT'CG_AA Cuts at: 4247 4247 Size: 5443 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5443 BstYI r'GATC_y Cuts at: 1972 2452 2773 3140 3525 3542 4994 5080 5092 Size: 480 321 367 385 17 1452 86 12 Cuts at: 5092 5438 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 480 385 367 346 321 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2118 513 Size: 552 1053 3838 Fragments arranged by size: 3838 1053 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2094 487 Size: 683 56 868 3836 Fragments arranged by size: 3836 868 683 56 BtsI GCAGTG_nn' Cuts at: 2401 2959 3753 3773 2401 Size: 558 794 20 4071 Fragments arranged by size: 4071 794 558 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5443 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5408 DraI TTT'AAA Cuts at: 2747 2747 Size: 5443 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4450 809 Size: 507 3134 1802 Fragments arranged by size: 3134 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 2670 2694 Size: 54 1425 50 275 18 216 624 24 Cuts at: 2694 2890 3779 5408 8 Size: 196 889 1629 43 Fragments arranged by size: 1629 1425 889 624 275 216 196 54 50 43 24 18 EagI C'GGCC_G Cuts at: 1537 1812 2670 2890 1537 Size: 275 858 220 4090 Fragments arranged by size: 4090 858 275 220 EarI CTCTTCn'nnn_ Cuts at: 1353 2064 3373 1353 Size: 711 1309 3423 Fragments arranged by size: 3423 1309 711 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2639 4030 4404 4550 387 Size: 1064 138 1050 1391 374 146 1280 Fragments arranged by size: 1391 1280 1064 1050 374 146 138 EcoICRI GAG'CTC Cuts at: 2212 2485 2212 Size: 273 5170 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2564 2685 3506 4103 4890 5158 1239 Size: 393 932 121 821 597 787 268 1524 Fragments arranged by size: 1524 932 821 787 597 393 268 121 EcoNI CCTnn'n_nnAGG Cuts at: 2597 2597 Size: 5443 EcoO109I rG'GnC_Cy Cuts at: 2140 2327 2590 2140 Size: 187 263 4993 Fragments arranged by size: 4993 263 187 EcoRI G'AATT_C Cuts at: 2752 3130 2752 Size: 378 5065 FspI TGC'GCA Cuts at: 3949 3949 Size: 5443 HaeII r_GCGC'y Cuts at: 1678 2092 2273 4590 1678 Size: 414 181 2317 2531 Fragments arranged by size: 2531 2317 414 181 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4084 4116 4158 4190 1373 Size: 32 135 32 2512 32 42 32 2626 Fragments arranged by size: 2626 2512 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2787 3033 669 Size: 988 281 849 246 3079 Fragments arranged by size: 3079 988 849 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5443 HpaI GTT'AAC Cuts at: 3033 3033 Size: 5443 KpnI G_GTAC'C Cuts at: 2446 2768 2446 Size: 322 5121 MfeI C'AATT_G Cuts at: 3042 3042 Size: 5443 MluI A'CGCG_T Cuts at: 5319 5319 Size: 5443 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4557 4741 1305 Size: 3252 184 2007 Fragments arranged by size: 3252 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3621 Fragments arranged by size: 3621 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3401 3760 3919 518 Size: 648 2235 359 159 2042 Fragments arranged by size: 2235 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2014 2365 2859 3536 4684 4929 1606 Size: 408 351 494 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 494 408 351 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4891 NdeI CA'TA_TG Cuts at: 387 387 Size: 5443 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5443 NotI GC'GGCC_GC Cuts at: 2890 2890 Size: 5443 NspI r_CATG'y Cuts at: 1132 1229 2801 4346 1132 Size: 97 1572 1545 2229 Fragments arranged by size: 2229 1572 1545 97 PasI CC'CwG_GG Cuts at: 1348 2323 1348 Size: 975 4468 PciI A'CATG_T Cuts at: 4342 4342 Size: 5443 PflMI CCAn_nnn'nTGG Cuts at: 1108 2044 1108 Size: 936 4507 PfoI T'CCnGG_A Cuts at: 2223 2223 Size: 5443 PmeI GTTT'AAAC Cuts at: 2747 2747 Size: 5443 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3512 3544 5025 5057 3512 Size: 32 1481 32 3898 Fragments arranged by size: 3898 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2327 2590 2327 Size: 263 5180 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5443 PsiI TTA'TAA Cuts at: 3013 3013 Size: 5443 PstI C_TGCA'G Cuts at: 830 2268 2451 2566 2795 830 Size: 1438 183 115 229 3478 Fragments arranged by size: 3478 1438 229 183 115 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5443 PvuII CAG'CTG Cuts at: 2014 2014 Size: 5443 SacI G_AGCT'C Cuts at: 2214 2487 2214 Size: 273 5170 SalI G'TCGA_C Cuts at: 1936 2785 1936 Size: 849 4594 SbfI CC_TGCA'GG Cuts at: 2451 2795 2451 Size: 344 5099 ScaI AGT'ACT Cuts at: 3691 5403 3691 Size: 1712 3731 SfcI C'TryA_G Cuts at: 826 1045 2264 2447 2562 2791 3926 4607 4798 Size: 219 1219 183 115 229 1135 681 191 Cuts at: 4798 826 Size: 1471 Fragments arranged by size: 1471 1219 1135 681 229 219 191 183 115 SfiI GGCCn_nnn'nGGCC Cuts at: 2129 2138 2129 Size: 9 5434 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5443 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5443 SmaI CCC'GGG Cuts at: 1872 2770 1872 Size: 898 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3547 4448 4710 820 Size: 197 926 1604 901 262 1553 Fragments arranged by size: 1604 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3234 493 Size: 2741 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5443 SphI G_CATG'C Cuts at: 1132 2801 1132 Size: 1669 3774 SspI AAT'ATT Cuts at: 5 52 3367 5 Size: 47 3315 2081 Fragments arranged by size: 3315 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5443 StyI C'CwwG_G Cuts at: 513 1065 2244 2882 513 Size: 552 1179 638 3074 Fragments arranged by size: 3074 1179 638 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2568 3470 3487 3640 3825 4270 325 Size: 1255 988 902 17 153 185 445 1498 Fragments arranged by size: 1498 1255 988 902 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3689 5401 96 Size: 274 80 33 51 1443 1712 1712 138 Fragments arranged by size: 1712 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5403 5435 5403 Size: 32 5411 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5443 XbaI T'CTAG_A Cuts at: 2779 2779 Size: 5443 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5443 XmaI C'CCGG_G Cuts at: 1870 2768 1870 Size: 898 4545 XmnI GAAnn'nnTTC Cuts at: 2357 3572 2357 Size: 1215 4228 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3251 276 Size: 53 83 186 936 1717 2468 Fragments arranged by size: 2468 1717 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfoI SrfI SwaI XcmI