################################## # NO CUT ENZYMES ( 169 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflIII AgeI AhdI Alw44I AlwI AlwNI AocI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BglI BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr13I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI HphI KasI KpnI KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MspA1I MunI NaeI NarI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccII 54 CGCG CG^CG AflII 673 CTTAAG C^TTAAG Aor51HI 522 AGCGCT AGC^GCT BbvI 4 GCTGC GCAGC(8/12) BcnI 552 CCGGG CC^[GC]GG BfrI 673 CTTAAG C^TTAAG BglII 502 AGATCT A^GATCT BmyI 912 GTGCCC G[AGT]GC[ACT]^C BsaI 145 GAGACC GGTCTC(1/5) BsaJI 575 CCATGG C^C[ACGT][ACGT]GG BsgI 921 GTGCAG GTGCAG(16/14) BsiYI 90 CCTGGTATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 90 CCTGGTATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG Bsp1286I 912 GTGCCC G[AGT]GC[ACT]^C BspHI 666 TCATGA T^CATGA BspMI 67 ACCTGC ACCTGC(4/8) BsrDI 305 GCAATG GCAATG(2/0) BsrFI 85 ACCGGC [GA]^CCGG[CT] BstNI 90 CCTGG CC^[AT]GG BstUI 54 CGCG CG^CG BstXI 83 CCACCGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 502 AGATCT [GA]^GATC[CT] Cac8I 923 GCAGGC GC[ACGT]^[ACGT]GC CfoI 523 GCGC GCG^C Cfr10I 85 ACCGGC [GA]^CCGG[CT] DsaI 575 CCATGG C^C[GA][CT]GG Eco47III 522 AGCGCT AGC^GCT EcoRII 90 CCTGG ^CC[AT]GG EcoT14I 575 CCATGG C^C[AT][AT]GG HaeII 522 AGCGCT [GA]GCGC^[CT] HaeIII 88 GGCC GG^CC HhaI 523 GCGC GCG^C HinP1I 523 GCGC G^CGC MflI 502 AGATCT [GA]^GATC[CT] MvaI 90 CCTGG CC^[AT]GG MvnI 54 CGCG CG^CG MwoI 914 GCCCAATGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 552 CCGGG CC^[GC]GG NcoI 575 CCATGG C^CATGG RcaI 666 TCATGA T^CATGA SfaNI 382 GATGC GCATC(5/9) StyI 575 CCATGG C^C[AT][AT]GG TseI 4 GCTGC G^C[AT]GC Tsp45I 79 GTGAC ^GT[GC]AC XhoII 502 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 135 AAATTC [GA]^AATT[CT] 557 AAATTT ApoI 135 AAATTC [GA]^AATT[CT] 557 AAATTT BbsI 481 GAAGAC GAAGAC(2/6) 987 GAAGAC BpuAI 481 GAAGAC GAAGAC(2/6) 987 GAAGAC BsmAI 145 GAGAC GTCTC(1/5) 183 GAGAC BsmFI 771 GGGAC GGGAC(10/14) 838 GGGAC BssKI 90 CCTGG ^CC[ACGT]GG 552 CCGGG Fnu4HI 4 GCTGC GC^[ACGT]GC 39 GCCGC HapII 86 CCGG C^CGG 552 CCGG HpaII 86 CCGG C^CGG 552 CCGG ItaI 4 GCTGC GC^[ACGT]GC 39 GCCGC MseI 425 TTAA T^TAA 674 TTAA MslI 51 CATCGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 875 CATGCCTGTG MspI 86 CCGG C^CGG 552 CCGG PleI 773 GACTC GAGTC(4/5) 990 GACTC PstI 331 CTGCAG CTGCA^G 621 CTGCAG ScrFI 90 CCTGG CC^[ACGT]GG 552 CCGGG SfcI 331 CTGCAG C^T[GA][CT]AG 621 CTGCAG SmlI 653 CTCAAG C^T[CT][GA]AG 673 CTTAAG SspI 627 AATATT AAT^ATT 638 AATATT Tru9I 425 TTAA T^TAA 674 TTAA TspRI 584 CAGTG CA[GC]TG[ACGT][ACGT]^ 910 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 22 enzymes) # ######################################### AciI AfaI AluI BsrI BstF5I DdeI DpnII EarI FokI HinfI Ksp632I MaeIII MboI MboII MnlI NdeII NlaIII NspI RsaI Sau3AI TfiI Tsp509I