################################## # NO CUT ENZYMES ( 112 enzymes) # ################################## Acc65I AccI AclI AflII AgeI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspI AvrII BamHI BbeI BbrPI BbvCI BciVI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsiWI BsmBI BsmI Bsp1407I BspDI BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraII DrdI DsaI EagI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI HgaI HincII HindII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SspBI StyI SwaI Tth111I XbaI XcmI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AatII 658 GACGTC GACGT^C AccIII 977 TCCGGA T^CCGGA AcyI 658 GACGTC G[GA]^CG[CT]C AhdI 1560 GACTATATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 118 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1280 CCTCAGG CC^T[ACGT]AGG Asp700I 1632 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1560 GACTATATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 1643 TGCGCA TGC^GCA BanII 1503 GAGCTC G[GA]GC[CT]^C BcgI 266 GCAAGGGTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 451 GCCCTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 658 GACGTC G[GA]^CG[CT]C BsaWI 977 TCCGGA [AT]^CCGG[AT] BseAI 977 TCCGGA T^CCGGA BseRI 421 GAGGAG GAGGAG(10/8) BsgI 313 GTGCAG GTGCAG(16/14) BsiEI 1069 CGGTCG CG[GA][CT]^CG BsmAI 1389 GTCTC GTCTC(1/5) BspEI 977 TCCGGA T^CCGGA BspHI 1815 TCATGA T^CATGA BsrDI 1647 CATTGC GCAATG(2/0) BsrFI 1307 ACCGGC [GA]^CCGG[CT] Bsu36I 1280 CCTCAGG CC^T[ACGT]AGG Cfr10I 1307 ACCGGC [GA]^CCGG[CT] DraIII 885 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 1560 GACTATATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1280 CCTCAGG CC^T[ACGT]AGG FspI 1643 TGCGCA TGC^GCA HaeII 1112 AGCGCC [GA]GCGC^[CT] Hin1I 658 GACGTC G[GA]^CG[CT]C HphI 114 TCACC GGTGA(8/7) MaeII 659 ACGT A^CGT MroI 977 TCCGGA T^CCGGA PaeR7I 1500 CTCGAG C^TCGAG PvuII 1057 CAGCTG CAG^CTG RcaI 1815 TCATGA T^CATGA SacI 1503 GAGCTC GAGCT^C SapI 1505 GCTCTTC GCTCTTC(1/4) SbfI 453 CCTGCAGG CCTGCA^GG Sse8387I 453 CCTGCAGG CCTGCA^GG SspI 233 AATATT AAT^ATT XhoI 1500 CTCGAG C^TCGAG XmnI 1632 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccII 1073 CGCG CG^CG 1445 CGCG AflIII 198 ACATGT A^C[GA][CT]GT 1409 ACATGT AlwI 1365 GGATC GGATC(4/5) 1806 GGATC AspHI 412 GTGCTC G[AT]GC[AT]^C 1503 GAGCTC BalI 1533 TGGCCA TGG^CCA 1695 TGGCCA BanI 377 GGCACC G^G[CT][GA]CC 890 GGTGCC BbsI 1201 GAAGAC GAAGAC(2/6) 1491 GTCTTC BclI 296 TGATCA T^GATCA 341 TGATCA BpuAI 1201 GAAGAC GAAGAC(2/6) 1491 GTCTTC BsiHKAI 412 GTGCTC G[AT]GC[AT]^C 1503 GAGCTC BsmFI 1187 GGGAC GGGAC(10/14) 1119 GTCCC BspLU11I 198 ACATGT A^CATGT 1409 ACATGT BstUI 1073 CGCG CG^CG 1445 CGCG BstXI 162 CCACCTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 241 CCAAAAGAATGG BstYI 1365 GGATCT [GA]^GATC[CT] 1806 GGATCT EaeI 1533 TGGCCA [CT]^GGCC[GA] 1695 TGGCCA FbaI 296 TGATCA T^GATCA 341 TGATCA HindIII 223 AAGCTT A^AGCTT 1525 AAGCTT MflI 1365 GGATCT [GA]^GATC[CT] 1806 GGATCT MluNI 1533 TGGCCA TGG^CCA 1695 TGGCCA MscI 1533 TGGCCA TGG^CCA 1695 TGGCCA MslI 304 CACTTCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1434 CATGCTCATG MspA1I 1057 CAGCTG C[AC]G^C[GT]G 1080 CAGCGG MvnI 1073 CGCG CG^CG 1445 CGCG PflMI 117 CCAGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1698 CCATGAACTGG ScaI 317 AGTACT AGT^ACT 1540 AGTACT SmaI 1184 CCCGGG CCC^GGG 1361 CCCGGG SmlI 1500 CTCGAG C^T[CT][GA]AG 1602 CTTGAG StuI 425 AGGCCT AGG^CCT 1716 AGGCCT Van91I 117 CCAGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1698 CCATGAACTGG XhoII 1365 GGATCT [GA]^GATC[CT] 1806 GGATCT XmaCI 1184 CCCGGG C^CCGGG 1361 CCCGGG XmaI 1184 CCCGGG C^CCGGG 1361 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI AvaI AvaII BbvI BcnI BmyI BpmI BsaJI BsiYI BslI BsoBI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EarI EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI Ksp632I MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I