(Circular) (Six-base) MAPSORT of: pFN21AE4139.seq Check: 163 from: 1 to: 6370 pFN21AE4139 6370 bp With 182 enzymes: SgfI * March 28, 2012 14:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4180 278 Size: 53 83 186 936 2644 2468 Fragments arranged by size: 2644 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3178 3713 1937 Size: 1241 535 4594 Fragments arranged by size: 4594 1241 535 Acc65I G'GTAC_C Cuts at: 3691 3691 Size: 6370 AclI AA'CG_TT Cuts at: 1563 4497 4870 1563 Size: 2934 373 3063 Fragments arranged by size: 3063 2934 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2594 3640 4433 5817 6085 1632 Size: 962 1046 793 1384 268 1917 Fragments arranged by size: 1917 1384 1046 962 793 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6173 AflIII A'CryG_T Cuts at: 1203 5269 6246 1203 Size: 4066 977 1327 Fragments arranged by size: 4066 1327 977 AgeI A'CCGG_T Cuts at: 5201 5201 Size: 6370 AhdI GACnn_n'nnGTC Cuts at: 5099 5099 Size: 6370 AleI CACnn'nnGTG Cuts at: 1166 2298 1166 Size: 1132 5238 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2952 3014 3385 1184 Size: 1768 62 371 4169 Fragments arranged by size: 4169 1768 371 62 AlwNI CAG_nnn'CTG Cuts at: 2107 2725 4113 5685 2107 Size: 618 1388 1572 2792 Fragments arranged by size: 2792 1572 1388 618 ApaLI G'TGCA_C Cuts at: 2234 3370 4427 5583 2234 Size: 1136 1057 1156 3021 Fragments arranged by size: 3021 1156 1136 1057 ApoI r'AATT_y Cuts at: 1493 2381 2429 2715 3679 3905 4057 6166 1493 Size: 888 48 286 964 226 152 2109 1697 Fragments arranged by size: 2109 1697 964 888 286 226 152 48 AseI AT'TA_AT Cuts at: 160 1032 4924 160 Size: 872 3892 1606 Fragments arranged by size: 3892 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6370 AvaI C'yCGr_G Cuts at: 1870 1943 2055 2070 3695 1870 Size: 73 112 15 1625 4545 Fragments arranged by size: 4545 1625 112 73 15 BamHI G'GATC_C Cuts at: 3700 4067 3700 Size: 367 6003 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2257 3691 5146 618 Size: 325 217 627 470 1434 1455 1842 Fragments arranged by size: 1842 1455 1434 627 470 325 217 BanII G_rGCy'C Cuts at: 1391 2050 3452 3689 1391 Size: 659 1402 237 4072 Fragments arranged by size: 4072 1402 659 237 BbeI G_GCGC'C Cuts at: 2261 2261 Size: 6370 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3434 3604 3669 928 Size: 971 1535 170 65 3629 Fragments arranged by size: 3629 1535 971 170 65 BbvCI CC'TCA_GC Cuts at: 2678 2678 Size: 6370 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4561 4595 1903 Size: 34 2624 34 3678 Fragments arranged by size: 3678 2624 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3565 4262 5472 3565 Size: 697 1210 4463 Fragments arranged by size: 4463 1210 697 BclI T'GATC_A Cuts at: 1548 1548 Size: 6370 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2075 2266 4981 Size: 107 122 71 631 605 403 191 2715 Cuts at: 4981 136 Size: 1525 Fragments arranged by size: 2715 1525 631 605 403 191 122 107 71 BglII A'GATC_T Cuts at: 3319 6365 3319 Size: 3046 3324 BlpI GC'TnA_GC Cuts at: 3787 3787 Size: 6370 Bme1580I G_kGCm'C Cuts at: 1790 2238 3374 3418 4431 5587 1790 Size: 448 1136 44 1013 1156 2573 Fragments arranged by size: 2573 1156 1136 1013 448 44 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6370 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3168 4101 5059 Size: 416 53 83 399 24 1751 933 958 Cuts at: 5059 442 Size: 1753 Fragments arranged by size: 1753 1751 958 933 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6370 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2517 2930 5030 1239 Size: 1278 413 2100 2579 Fragments arranged by size: 2579 2100 1278 413 Bpu10I CC'TnA_GC Cuts at: 2678 2678 Size: 6370 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4495 5360 5658 4495 Size: 865 298 5207 Fragments arranged by size: 5207 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5033 882 Size: 617 3534 2219 Fragments arranged by size: 3534 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4161 493 Size: 711 2957 2702 Fragments arranged by size: 2957 2702 711 BsaBI GATnn'nnATC Cuts at: 3525 6364 3525 Size: 2839 3531 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2258 4177 4559 5194 Size: 53 83 186 936 725 1919 382 635 Cuts at: 5194 275 Size: 1451 Fragments arranged by size: 1919 1451 936 725 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2592 4803 5201 5475 5622 6033 2592 Size: 2211 398 274 147 411 2929 Fragments arranged by size: 2929 2211 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6145 Fragments arranged by size: 6145 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6370 BseYI C'CCAG_C Cuts at: 2219 2331 5573 6069 2219 Size: 112 3242 496 2520 Fragments arranged by size: 3242 2520 496 112 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2912 1163 Size: 573 1176 4621 Fragments arranged by size: 4621 1176 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2039 2150 2266 3820 4581 5609 Size: 275 186 38 111 116 1554 761 1028 Cuts at: 5609 1540 Size: 2301 Fragments arranged by size: 2301 1554 1028 761 275 186 116 111 38 BsiHKAI G_wGCw'C Cuts at: 2050 2238 2850 3107 3374 4431 4516 5587 2050 Size: 188 612 257 267 1057 85 1071 2833 Fragments arranged by size: 2833 1071 1057 612 267 257 188 85 BsmI GAATG_Cn' Cuts at: 3881 3974 3881 Size: 93 6277 BsmBI CGTCTCn'nnnn_ Cuts at: 2359 2359 Size: 6370 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2050 2238 2850 3107 3374 3418 3452 Size: 399 260 188 612 257 267 44 34 Cuts at: 3452 3689 4431 4516 5587 1391 Size: 237 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 612 399 267 260 257 237 188 85 44 34 BspEI T'CCGG_A Cuts at: 2592 6033 2592 Size: 3441 2929 BspHI T'CATG_A Cuts at: 4259 5989 4259 Size: 1730 4640 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3541 3725 844 Size: 2697 184 3489 Fragments arranged by size: 3489 2697 184 BsrBI CCG'CTC Cuts at: 1394 1823 2454 4257 1394 Size: 429 631 1803 3507 Fragments arranged by size: 3507 1803 631 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3426 3432 4865 5039 65 Size: 1183 2178 6 1433 174 1396 Fragments arranged by size: 2178 1433 1396 1183 174 6 BsrFI r'CCGG_y Cuts at: 1700 1810 2156 2163 2254 2265 5014 5201 1700 Size: 110 346 7 91 11 2749 187 2869 Fragments arranged by size: 2869 2749 346 187 110 91 11 7 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6370 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6370 BssSI C'ACGA_G Cuts at: 4430 5442 4430 Size: 1012 5358 BstAPI GCAn_nnn'nTGC Cuts at: 794 2107 794 Size: 1313 5057 BstBI TT'CG_AA Cuts at: 5174 5174 Size: 6370 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6370 BstXI CCAn_nnnn'nTGG Cuts at: 2842 2842 Size: 6370 BstYI r'GATC_y Cuts at: 1972 3319 3343 3526 3700 4067 4452 4469 5921 Size: 1347 24 183 174 367 385 17 1452 Cuts at: 5921 6007 6019 6365 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1347 385 367 346 183 174 86 24 17 12 BstZ17I GTA'TAC Cuts at: 3179 3179 Size: 6370 BtgI C'CryG_G Cuts at: 513 1065 2361 2841 513 Size: 552 1296 480 4042 Fragments arranged by size: 4042 1296 552 480 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5631 Fragments arranged by size: 5631 683 56 BtsI GCAGTG_nn' Cuts at: 3886 4680 4700 3886 Size: 794 20 5556 Fragments arranged by size: 5556 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6370 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6335 DraI TTT'AAA Cuts at: 3674 3674 Size: 6370 DraIII CAC_nnn'GTG Cuts at: 3411 3608 3411 Size: 197 6173 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5377 809 Size: 507 4061 1802 Fragments arranged by size: 4061 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2147 3510 3817 Size: 54 1425 50 275 18 317 1363 307 Cuts at: 3817 4706 6335 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1363 889 317 307 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2147 3817 1537 Size: 275 335 1670 4090 Fragments arranged by size: 4090 1670 335 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2315 3024 3390 4300 1353 Size: 962 709 366 910 3423 Fragments arranged by size: 3423 962 910 709 366 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4957 5331 5477 387 Size: 1064 138 3368 374 146 1280 Fragments arranged by size: 3368 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2048 2048 Size: 6370 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2517 2594 2930 3640 4433 5030 5817 Size: 393 885 77 336 710 793 597 787 Cuts at: 5817 6085 1239 Size: 268 1524 Fragments arranged by size: 1524 885 793 787 710 597 393 336 268 77 EcoNI CCTnn'n_nnAGG Cuts at: 2681 2681 Size: 6370 EcoO109I rG'GnC_Cy Cuts at: 2515 2515 Size: 6370 EcoRI G'AATT_C Cuts at: 2381 2429 3679 4057 2381 Size: 48 1250 378 4694 Fragments arranged by size: 4694 1250 378 48 EcoRV GAT'ATC Cuts at: 3139 3139 Size: 6370 FspI TGC'GCA Cuts at: 2393 2552 4876 2393 Size: 159 2324 3887 Fragments arranged by size: 3887 2324 159 HaeII r_GCGC'y Cuts at: 1678 2208 2261 5517 1678 Size: 530 53 3256 2531 Fragments arranged by size: 3256 2531 530 53 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3230 3262 3539 3571 3584 Size: 32 135 32 1658 32 277 32 13 Cuts at: 3584 3616 5011 5043 5085 5117 1373 Size: 32 1395 32 42 32 2626 Fragments arranged by size: 2626 1658 1395 277 135 42 32 32 32 32 32 32 32 13 HincII GTy'rAC Cuts at: 669 1657 1938 3572 3714 3960 669 Size: 988 281 1634 142 246 3079 Fragments arranged by size: 3079 1634 988 281 246 142 HindIII A'AGCT_T Cuts at: 748 3652 748 Size: 2904 3466 HpaI GTT'AAC Cuts at: 3960 3960 Size: 6370 KasI G'GCGC_C Cuts at: 2257 2257 Size: 6370 KpnI G_GTAC'C Cuts at: 3695 3695 Size: 6370 MfeI C'AATT_G Cuts at: 3969 3969 Size: 6370 MluI A'CGCG_T Cuts at: 6246 6246 Size: 6370 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5484 5668 1305 Size: 4179 184 2007 Fragments arranged by size: 4179 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4548 Fragments arranged by size: 4548 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2298 3367 4328 4687 4846 518 Size: 648 1132 1069 961 359 159 2042 Fragments arranged by size: 2042 1132 1069 961 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2107 2363 2963 3786 4463 5611 5856 1606 Size: 501 256 600 823 677 1148 245 2120 Fragments arranged by size: 2120 1148 823 677 600 501 256 245 NaeI GCC'GGC Cuts at: 2158 2165 2256 2158 Size: 7 91 6272 Fragments arranged by size: 6272 91 7 NarI GG'CG_CC Cuts at: 2258 2258 Size: 6370 NcoI C'CATG_G Cuts at: 513 1065 2841 513 Size: 552 1776 4042 Fragments arranged by size: 4042 1776 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6370 NgoMIV G'CCGG_C Cuts at: 2156 2163 2254 2156 Size: 7 91 6272 Fragments arranged by size: 6272 91 7 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6370 NotI GC'GGCC_GC Cuts at: 3817 3817 Size: 6370 NspI r_CATG'y Cuts at: 1132 1229 3067 3584 3728 5273 1132 Size: 97 1838 517 144 1545 2229 Fragments arranged by size: 2229 1838 1545 517 144 97 PasI CC'CwG_GG Cuts at: 1348 2465 3559 1348 Size: 1117 1094 4159 Fragments arranged by size: 4159 1117 1094 PciI A'CATG_T Cuts at: 5269 5269 Size: 6370 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6370 PmeI GTTT'AAAC Cuts at: 3674 3674 Size: 6370 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4439 4471 5952 5984 4439 Size: 32 1481 32 4825 Fragments arranged by size: 4825 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6370 PsiI TTA'TAA Cuts at: 3940 3940 Size: 6370 PstI C_TGCA'G Cuts at: 830 2103 2523 2902 3722 830 Size: 1273 420 379 820 3478 Fragments arranged by size: 3478 1273 820 420 379 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6370 PvuII CAG'CTG Cuts at: 2107 2963 2107 Size: 856 5514 SacI G_AGCT'C Cuts at: 2050 2050 Size: 6370 SacII CC_GC'GG Cuts at: 2364 2364 Size: 6370 SalI G'TCGA_C Cuts at: 1936 3712 1936 Size: 1776 4594 SbfI CC_TGCA'GG Cuts at: 3722 3722 Size: 6370 ScaI AGT'ACT Cuts at: 4618 6330 4618 Size: 1712 4658 SfcI C'TryA_G Cuts at: 826 1045 2099 2519 2898 3718 4853 5534 5725 Size: 219 1054 420 379 820 1135 681 191 Cuts at: 5725 826 Size: 1471 Fragments arranged by size: 1471 1135 1054 820 681 420 379 219 191 SfoI GGC'GCC Cuts at: 2259 2259 Size: 6370 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6370 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6370 SmaI CCC'GGG Cuts at: 1872 2072 3697 1872 Size: 200 1625 4545 Fragments arranged by size: 4545 1625 200 SmlI C'TyrA_G Cuts at: 820 1017 1943 4474 5375 5637 820 Size: 197 926 2531 901 262 1553 Fragments arranged by size: 2531 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4161 493 Size: 3668 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6370 SphI G_CATG'C Cuts at: 1132 3584 3728 1132 Size: 2452 144 3774 Fragments arranged by size: 3774 2452 144 SspI AAT'ATT Cuts at: 5 52 2872 4294 5 Size: 47 2820 1422 2081 Fragments arranged by size: 2820 2081 1422 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6370 StyI C'CwwG_G Cuts at: 513 1065 2841 2940 3499 3575 3809 513 Size: 552 1776 99 559 76 234 3074 Fragments arranged by size: 3074 1776 559 552 234 99 76 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4397 4414 4567 4752 5197 325 Size: 1255 2817 17 153 185 445 1498 Fragments arranged by size: 2817 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4616 6328 96 Size: 274 80 33 51 1443 2639 1712 138 Fragments arranged by size: 2639 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6330 6362 6330 Size: 32 6338 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6370 XbaI T'CTAG_A Cuts at: 3706 3706 Size: 6370 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6370 XmaI C'CCGG_G Cuts at: 1870 2070 3695 1870 Size: 200 1625 4545 Fragments arranged by size: 4545 1625 200 XmnI GAAnn'nnTTC Cuts at: 4499 4499 Size: 6370 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4178 276 Size: 53 83 186 936 2644 2468 Fragments arranged by size: 2644 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI Bsu36I FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI XcmI