(Linear) (Six-base) MAPSORT of: KIEE4139.seq Check: 9479 from: 1 to: 1665 KIEE4139.orf With 182 enzymes: SgfI * March 22, 2012 17:27 .. AccI GT'mk_AC Cuts at: 0 1173 1665 Size: 1173 492 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 589 1635 1665 Size: 589 1046 30 Fragments arranged by size: 1046 589 30 AleI CACnn'nnGTG Cuts at: 0 293 1665 Size: 293 1372 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 947 1009 1380 1665 Size: 947 62 371 285 Fragments arranged by size: 947 371 285 62 AlwNI CAG_nnn'CTG Cuts at: 0 102 720 1665 Size: 102 618 945 Fragments arranged by size: 945 618 102 ApaLI G'TGCA_C Cuts at: 0 229 1365 1665 Size: 229 1136 300 Fragments arranged by size: 1136 300 229 ApoI r'AATT_y Cuts at: 0 376 424 710 1665 Size: 376 48 286 955 Fragments arranged by size: 955 376 286 48 AvaI C'yCGr_G Cuts at: 0 50 65 1665 Size: 50 15 1600 Fragments arranged by size: 1600 50 15 BanI G'GyrC_C Cuts at: 0 252 1665 Size: 252 1413 BanII G_rGCy'C Cuts at: 0 45 1447 1665 Size: 45 1402 218 Fragments arranged by size: 1402 218 45 BbeI G_GCGC'C Cuts at: 0 256 1665 Size: 256 1409 BbsI GAAGACnn'nnnn_ Cuts at: 0 1429 1599 1664 1665 Size: 1429 170 65 1 Fragments arranged by size: 1429 170 65 1 BbvCI CC'TCA_GC Cuts at: 0 673 1665 Size: 673 992 BciVI GTATCCnnnnn_n' Cuts at: 0 1560 1665 Size: 1560 105 BglI GCCn_nnn'nGGC Cuts at: 0 70 261 1665 Size: 70 191 1404 Fragments arranged by size: 1404 191 70 BglII A'GATC_T Cuts at: 0 1314 1665 Size: 1314 351 Bme1580I G_kGCm'C Cuts at: 0 233 1369 1413 1665 Size: 233 1136 44 252 Fragments arranged by size: 1136 252 233 44 BmrI ACTGGGnnnn_n' Cuts at: 0 1163 1665 Size: 1163 502 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 512 925 1665 Size: 512 413 740 Fragments arranged by size: 740 512 413 Bpu10I CC'TnA_GC Cuts at: 0 673 1665 Size: 673 992 BsaBI GATnn'nnATC Cuts at: 0 1520 1665 Size: 1520 145 BsaHI Gr'CG_yC Cuts at: 0 253 1665 Size: 253 1412 BsaWI w'CCGG_w Cuts at: 0 587 1665 Size: 587 1078 BseYI C'CCAG_C Cuts at: 0 214 326 1665 Size: 214 112 1339 Fragments arranged by size: 1339 214 112 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 907 1665 Size: 907 758 BsiEI CG_ry'CG Cuts at: 0 34 145 261 1665 Size: 34 111 116 1404 Fragments arranged by size: 1404 116 111 34 BsiHKAI G_wGCw'C Cuts at: 0 45 233 845 1102 1369 1665 Size: 45 188 612 257 267 296 Fragments arranged by size: 612 296 267 257 188 45 BsmBI CGTCTCn'nnnn_ Cuts at: 0 354 1665 Size: 354 1311 Bsp1286I G_dGCh'C Cuts at: 0 45 233 845 1102 1369 1413 1447 1665 Size: 45 188 612 257 267 44 34 218 Fragments arranged by size: 612 267 257 218 188 45 44 34 BspEI T'CCGG_A Cuts at: 0 587 1665 Size: 587 1078 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1536 1665 Size: 1536 129 BsrBI CCG'CTC Cuts at: 0 449 1665 Size: 449 1216 BsrDI GCAATG_nn' Cuts at: 0 1421 1427 1665 Size: 1421 6 238 Fragments arranged by size: 1421 238 6 BsrFI r'CCGG_y Cuts at: 0 151 158 249 260 1665 Size: 151 7 91 11 1405 Fragments arranged by size: 1405 151 91 11 7 BstAPI GCAn_nnn'nTGC Cuts at: 0 102 1665 Size: 102 1563 BstXI CCAn_nnnn'nTGG Cuts at: 0 837 1665 Size: 837 828 BstYI r'GATC_y Cuts at: 0 1314 1338 1521 1665 Size: 1314 24 183 144 Fragments arranged by size: 1314 183 144 24 BstZ17I GTA'TAC Cuts at: 0 1174 1665 Size: 1174 491 BtgI C'CryG_G Cuts at: 0 356 836 1665 Size: 356 480 829 Fragments arranged by size: 829 480 356 DraIII CAC_nnn'GTG Cuts at: 0 1406 1603 1665 Size: 1406 197 62 Fragments arranged by size: 1406 197 62 EaeI y'GGCC_r Cuts at: 0 142 1505 1665 Size: 142 1363 160 Fragments arranged by size: 1363 160 142 EagI C'GGCC_G Cuts at: 0 142 1665 Size: 142 1523 EarI CTCTTCn'nnn_ Cuts at: 0 310 1019 1385 1665 Size: 310 709 366 280 Fragments arranged by size: 709 366 310 280 EcoICRI GAG'CTC Cuts at: 0 43 1665 Size: 43 1622 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 512 589 925 1635 1665 Size: 512 77 336 710 30 Fragments arranged by size: 710 512 336 77 30 EcoNI CCTnn'n_nnAGG Cuts at: 0 676 1665 Size: 676 989 EcoO109I rG'GnC_Cy Cuts at: 0 510 1665 Size: 510 1155 EcoRI G'AATT_C Cuts at: 0 376 424 1665 Size: 376 48 1241 Fragments arranged by size: 1241 376 48 EcoRV GAT'ATC Cuts at: 0 1134 1665 Size: 1134 531 FspI TGC'GCA Cuts at: 0 388 547 1665 Size: 388 159 1118 Fragments arranged by size: 1118 388 159 HaeII r_GCGC'y Cuts at: 0 203 256 1665 Size: 203 53 1409 Fragments arranged by size: 1409 203 53 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1225 1257 1534 1566 1579 1611 1665 Size: 1225 32 277 32 13 32 54 Fragments arranged by size: 1225 277 54 32 32 32 13 HincII GTy'rAC Cuts at: 0 1567 1665 Size: 1567 98 HindIII A'AGCT_T Cuts at: 0 1647 1665 Size: 1647 18 KasI G'GCGC_C Cuts at: 0 252 1665 Size: 252 1413 MslI CAynn'nnrTG Cuts at: 0 293 1362 1665 Size: 293 1069 303 Fragments arranged by size: 1069 303 293 MspA1I CmG'CkG Cuts at: 0 102 358 958 1665 Size: 102 256 600 707 Fragments arranged by size: 707 600 256 102 NaeI GCC'GGC Cuts at: 0 153 160 251 1665 Size: 153 7 91 1414 Fragments arranged by size: 1414 153 91 7 NarI GG'CG_CC Cuts at: 0 253 1665 Size: 253 1412 NcoI C'CATG_G Cuts at: 0 836 1665 Size: 836 829 NgoMIV G'CCGG_C Cuts at: 0 151 158 249 1665 Size: 151 7 91 1416 Fragments arranged by size: 1416 151 91 7 NspI r_CATG'y Cuts at: 0 1062 1579 1665 Size: 1062 517 86 Fragments arranged by size: 1062 517 86 PasI CC'CwG_GG Cuts at: 0 460 1554 1665 Size: 460 1094 111 Fragments arranged by size: 1094 460 111 PstI C_TGCA'G Cuts at: 0 98 518 897 1665 Size: 98 420 379 768 Fragments arranged by size: 768 420 379 98 PvuII CAG'CTG Cuts at: 0 102 958 1665 Size: 102 856 707 Fragments arranged by size: 856 707 102 SacI G_AGCT'C Cuts at: 0 45 1665 Size: 45 1620 SacII CC_GC'GG Cuts at: 0 359 1665 Size: 359 1306 SfcI C'TryA_G Cuts at: 0 94 514 893 1665 Size: 94 420 379 772 Fragments arranged by size: 772 420 379 94 SfoI GGC'GCC Cuts at: 0 254 1665 Size: 254 1411 SmaI CCC'GGG Cuts at: 0 67 1665 Size: 67 1598 SphI G_CATG'C Cuts at: 0 1579 1665 Size: 1579 86 SspI AAT'ATT Cuts at: 0 867 1665 Size: 867 798 StyI C'CwwG_G Cuts at: 0 836 935 1494 1570 1665 Size: 836 99 559 76 95 Fragments arranged by size: 836 559 99 95 76 XmaI C'CCGG_G Cuts at: 0 65 1665 Size: 65 1600 Enzymes that do cut: AccI AcuI AleI AlfI AlwNI ApaLI ApoI AvaI BanI BanII BbeI BbsI BbvCI BciVI BglI BglII Bme1580I BmrI BpmI Bpu10I BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BstAPI BstXI BstYI BstZ17I BtgI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII KasI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PstI PvuII SacI SacII SfcI SfoI SmaI SphI SspI StyI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI BcgI BclI BfrBI BlpI BmgBI BmtI BplI BpuEI BsaI BsaAI BsaXI BseRI BsiWI BsmI BspHI BsrGI BssHII BssSI BstBI BstEII Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EciI FalI FseI FspAI HpaI KpnI MfeI MluI MmeI MscI NdeI NheI NotI NruI NsiI PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SrfI StuI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI