Restriction map for
pFN21AB8906
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspHI AspI AvrII BamHI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaWI
BseAI BseRI BsiHKAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraII
DraIII DrdI Eam1105I Eco47III Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI MaeII MaeIII MamI
MfeI MluI MroI MseI MslI
MunI NaeI NcoI NdeI NgoMIV
NheI NlaIII NotI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tru9I Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 320 GGTACC G^GTACC
AfaI 321 GTAC GT^AC
AlwNI 283 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 320 GGTACC G^GTACC
AvaII 643 GGTCC G^G[AT]CC
AviII 32 TGCGCA TGC^GCA
BalI 2 TGGCCA TGG^CCA
BanII 231 GGGCTC G[GA]GC[CT]^C
BbeI 595 GGCGCC GGCGC^C
BglII 386 AGATCT A^GATCT
BmyI 231 GGGCTC G[AGT]GC[ACT]^C
BsgI 475 CTGCAC GTGCAG(16/14)
BsiEI 205 CGGCCG CG[GA][CT]^CG
BsmBI 224 CGTCTC CGTCTC(1/5)
BsmFI 644 GTCCC GGGAC(10/14)
Bsp1286I 231 GGGCTC G[AGT]GC[ACT]^C
BsrBI 298 GAGCGG CCGCTC(-3/-3)
BsrFI 513 GCCGGT [GA]^CCGG[CT]
BsrI 713 ACTGG ACTGG(1/-1)
BssHII 420 GCGCGC G^CGCGC
BstAPI 66 GCAACACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 386 AGATCT [GA]^GATC[CT]
Cfr10I 513 GCCGGT [GA]^CCGG[CT]
DpnII 387 GATC ^GATC
DsaI 575 CCGCGG C^C[GA][CT]GG
EagI 205 CGGCCG C^GGCCG
EarI 664 CTCTTC CTCTTC(1/4)
EclXI 205 CGGCCG C^GGCCG
Eco52I 205 CGGCCG C^GGCCG
EcoT14I 275 CCAAGG C^C[AT][AT]GG
FspI 32 TGCGCA TGC^GCA
HphI 26 TCACC GGTGA(8/7)
KasI 595 GGCGCC G^GCGCC
KpnI 320 GGTACC GGTAC^C
Ksp632I 664 CTCTTC CTCTTC(1/4)
KspI 575 CCGCGG CCGC^GG
MboI 387 GATC ^GATC
MflI 386 AGATCT [GA]^GATC[CT]
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
NarI 595 GGCGCC GG^CGCC
NdeII 387 GATC ^GATC
NruI 525 TCGCGA TCG^CGA
PvuII 346 CAGCTG CAG^CTG
RsaI 321 GTAC GT^AC
SacII 575 CCGCGG CCGC^GG
Sau3AI 387 GATC ^GATC
SfoI 595 GGCGCC GGC^GCC
StyI 275 CCAAGG C^C[AT][AT]GG
XbaI 42 TCTAGA T^CTAGA
XhoII 386 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AvaI 52 CCCGAG C^[CT]CG[GA]G
334 CCCGAG
BfaI 43 CTAG C^TAG
215 CTAG
BsaI 178 GAGACC GGTCTC(1/5)
702 GGTCTC
BsoBI 52 CCCGAG C^[CT]CG[GA]G
334 CCCGAG
BspMI 71 ACCTGC ACCTGC(4/8)
378 GCAGGT
BstF5I 464 CATCC GGATG(2/0)
689 CATCC
DdeI 19 CTGAG C^T[ACGT]AG
684 CTTAG
FokI 464 CATCC GGATG(9/13)
689 CATCC
MaeI 43 CTAG C^TAG
215 CTAG
MboII 252 GAAGA GAAGA(8/7)
665 TCTTC
PleI 193 GACTC GAGTC(4/5)
445 GAGTC
SfaNI 463 GCATC GCATC(5/9)
688 GCATC
TfiI 133 GATTC G^A[AT]TC
399 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AccII AciI AcyI AluI BanI
BbvI BcnI BpmI BsaHI BsaJI
BsiYI BslI BsmAI BssKI BstNI
BstUI Cac8I CfoI Cfr13I EaeI
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII ItaI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIV Sau96I ScrFI TseI