(Circular) (Six-base) MAPSORT of: pFN21AB8906.seq Check: 9161 from: 1 to: 5422 pFN21AB8906 5422 bp With 182 enzymes: SgfI * December 8, 2014 13:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2085 2085 Size: 5422 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3232 278 Size: 53 83 186 936 1696 2468 Fragments arranged by size: 2468 1696 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2765 1937 Size: 828 4594 Acc65I G'GTAC_C Cuts at: 2325 2743 2325 Size: 418 5004 AclI AA'CG_TT Cuts at: 1563 3549 3922 1563 Size: 1986 373 3063 Fragments arranged by size: 3063 1986 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2441 3485 4869 5137 1632 Size: 809 1044 1384 268 1917 Fragments arranged by size: 1917 1384 1044 809 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5225 AflIII A'CryG_T Cuts at: 1203 4321 5298 1203 Size: 3118 977 1327 Fragments arranged by size: 3118 1327 977 AgeI A'CCGG_T Cuts at: 4253 4253 Size: 5422 AhdI GACnn_n'nnGTC Cuts at: 4151 4151 Size: 5422 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5422 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5422 AlwNI CAG_nnn'CTG Cuts at: 2293 3165 4737 2293 Size: 872 1572 2978 Fragments arranged by size: 2978 1572 872 ApaLI G'TGCA_C Cuts at: 3479 4635 3479 Size: 1156 4266 ApoI r'AATT_y Cuts at: 1493 2731 2957 3109 5218 1493 Size: 1238 226 152 2109 1697 Fragments arranged by size: 2109 1697 1238 226 152 AseI AT'TA_AT Cuts at: 160 1032 3976 160 Size: 872 2944 1606 Fragments arranged by size: 2944 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5422 AvaI C'yCGr_G Cuts at: 1870 1943 2057 2339 2747 1870 Size: 73 114 282 408 4545 Fragments arranged by size: 4545 408 282 114 73 BamHI G'GATC_C Cuts at: 2752 3119 2752 Size: 367 5055 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2325 2515 2600 2743 4198 Size: 325 217 627 538 190 85 143 1455 Cuts at: 4198 618 Size: 1842 Fragments arranged by size: 1842 1455 627 538 325 217 190 143 85 BanII G_rGCy'C Cuts at: 1391 2240 2741 1391 Size: 849 501 4072 Fragments arranged by size: 4072 849 501 BbeI G_GCGC'C Cuts at: 2604 2604 Size: 5422 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4451 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3613 3647 1903 Size: 34 1676 34 3678 Fragments arranged by size: 3678 1676 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3314 4524 3314 Size: 1210 4212 BclI T'GATC_A Cuts at: 1548 1548 Size: 5422 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4033 136 Size: 107 122 71 631 605 2361 1525 Fragments arranged by size: 2361 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2391 5417 2391 Size: 3026 2396 BlpI GC'TnA_GC Cuts at: 2839 2839 Size: 5422 Bme1580I G_kGCm'C Cuts at: 1790 3483 4639 1790 Size: 1693 1156 2573 Fragments arranged by size: 2573 1693 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5422 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2728 3153 4111 Size: 416 53 83 399 24 1311 425 958 Cuts at: 4111 442 Size: 1753 Fragments arranged by size: 1753 1311 958 425 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5422 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2201 2315 2421 2592 4082 1239 Size: 962 114 106 171 1490 2579 Fragments arranged by size: 2579 1490 962 171 114 106 Bpu10I CC'TnA_GC Cuts at: 2024 2024 Size: 5422 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3547 4412 4710 3547 Size: 865 298 4259 Fragments arranged by size: 4259 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2177 2713 4085 882 Size: 617 678 536 1372 2219 Fragments arranged by size: 2219 1372 678 617 536 BsaAI yAC'GTr Cuts at: 493 1204 3213 493 Size: 711 2009 2702 Fragments arranged by size: 2702 2009 711 BsaBI GATnn'nnATC Cuts at: 5416 5416 Size: 5422 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2020 2244 2601 3229 Size: 53 83 186 936 487 224 357 628 Cuts at: 3229 3611 4246 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 635 628 487 382 357 224 186 83 53 BsaWI w'CCGG_w Cuts at: 3855 4253 4527 4674 5085 3855 Size: 398 274 147 411 4192 Fragments arranged by size: 4192 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5197 Fragments arranged by size: 5197 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5422 BseYI C'CCAG_C Cuts at: 2109 4625 5121 2109 Size: 2516 496 2410 Fragments arranged by size: 2516 2410 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2465 1163 Size: 573 729 4120 Fragments arranged by size: 4120 729 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2213 2872 3633 4661 1540 Size: 275 186 212 659 761 1028 2301 Fragments arranged by size: 2301 1028 761 659 275 212 186 BsiHKAI G_wGCw'C Cuts at: 3483 3568 4639 3483 Size: 85 1071 4266 Fragments arranged by size: 4266 1071 85 BsmI GAATG_Cn' Cuts at: 2933 3026 2933 Size: 93 5329 BsmBI CGTCTCn'nnnn_ Cuts at: 2235 2235 Size: 5422 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2240 2741 3483 3568 4639 1391 Size: 399 450 501 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 501 450 399 85 BspEI T'CCGG_A Cuts at: 5085 5085 Size: 5422 BspHI T'CATG_A Cuts at: 3311 5041 3311 Size: 1730 3692 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2085 2374 2777 844 Size: 1241 289 403 3489 Fragments arranged by size: 3489 1241 403 289 BsrBI CCG'CTC Cuts at: 1394 1823 2305 3309 1394 Size: 429 482 1004 3507 Fragments arranged by size: 3507 1004 482 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3917 4091 65 Size: 1183 2669 174 1396 Fragments arranged by size: 2669 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2518 4066 4253 1700 Size: 110 708 1548 187 2869 Fragments arranged by size: 2869 1548 708 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5422 BssHII G'CGCG_C Cuts at: 1925 2425 1925 Size: 500 4922 BssSI C'ACGA_G Cuts at: 3482 4494 3482 Size: 1012 4410 BstAPI GCAn_nnn'nTGC Cuts at: 794 2077 794 Size: 1283 4139 BstBI TT'CG_AA Cuts at: 4226 4226 Size: 5422 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5422 BstYI r'GATC_y Cuts at: 1972 2391 2752 3119 3504 3521 4973 5059 5071 Size: 419 361 367 385 17 1452 86 12 Cuts at: 5071 5417 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 419 385 367 361 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2580 513 Size: 552 939 576 3355 Fragments arranged by size: 3355 939 576 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2393 487 Size: 683 56 1167 3516 Fragments arranged by size: 3516 1167 683 56 BtsI GCAGTG_nn' Cuts at: 2938 3732 3752 2938 Size: 794 20 4608 Fragments arranged by size: 4608 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5422 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5387 DraI TTT'AAA Cuts at: 2726 2726 Size: 5422 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4429 809 Size: 507 3113 1802 Fragments arranged by size: 3113 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2149 2210 Size: 54 1425 50 275 18 177 142 61 Cuts at: 2210 2869 3758 5387 8 Size: 659 889 1629 43 Fragments arranged by size: 1629 1425 889 659 275 177 142 61 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2210 2869 1537 Size: 275 398 659 4090 Fragments arranged by size: 4090 659 398 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2675 3352 1353 Size: 1322 677 3423 Fragments arranged by size: 3423 1322 677 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2461 2462 4009 4383 4529 387 Size: 1064 138 872 1 1547 374 146 1280 Fragments arranged by size: 1547 1280 1064 872 374 146 138 1 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2201 2315 2421 2441 2592 3485 4082 Size: 393 569 114 106 20 151 893 597 Cuts at: 4082 4869 5137 1239 Size: 787 268 1524 Fragments arranged by size: 1524 893 787 597 569 393 268 151 114 106 20 EcoRI G'AATT_C Cuts at: 2731 3109 2731 Size: 378 5044 FspI TGC'GCA Cuts at: 2039 3928 2039 Size: 1889 3533 HaeII r_GCGC'y Cuts at: 1678 2311 2604 2663 4569 1678 Size: 633 293 59 1906 2531 Fragments arranged by size: 2531 1906 633 293 59 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4063 4095 4137 4169 1373 Size: 32 135 32 2491 32 42 32 2626 Fragments arranged by size: 2626 2491 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2766 3012 669 Size: 988 281 828 246 3079 Fragments arranged by size: 3079 988 828 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5422 HpaI GTT'AAC Cuts at: 3012 3012 Size: 5422 KasI G'GCGC_C Cuts at: 2600 2600 Size: 5422 KpnI G_GTAC'C Cuts at: 2329 2747 2329 Size: 418 5004 MfeI C'AATT_G Cuts at: 3021 3021 Size: 5422 MluI A'CGCG_T Cuts at: 5298 5298 Size: 5422 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2280 4536 4720 1305 Size: 975 2256 184 2007 Fragments arranged by size: 2256 2007 975 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 3423 Fragments arranged by size: 3423 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 3380 3739 3898 518 Size: 648 2214 359 159 2042 Fragments arranged by size: 2214 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2209 2323 2353 2582 2838 3515 4663 4908 Size: 603 114 30 229 256 677 1148 245 Cuts at: 4908 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 603 256 245 229 114 30 NarI GG'CG_CC Cuts at: 2601 2601 Size: 5422 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3931 Fragments arranged by size: 3931 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5422 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5422 NotI GC'GGCC_GC Cuts at: 2869 2869 Size: 5422 NruI TCG'CGA Cuts at: 2532 2532 Size: 5422 NspI r_CATG'y Cuts at: 1132 1229 2780 4325 1132 Size: 97 1551 1545 2229 Fragments arranged by size: 2229 1551 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5422 PciI A'CATG_T Cuts at: 4321 4321 Size: 5422 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5422 PfoI T'CCnGG_A Cuts at: 2711 2711 Size: 5422 PmeI GTTT'AAAC Cuts at: 2726 2726 Size: 5422 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3491 3523 5004 5036 3491 Size: 32 1481 32 3877 Fragments arranged by size: 3877 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5422 PsiI TTA'TAA Cuts at: 2992 2992 Size: 5422 PstI C_TGCA'G Cuts at: 830 2774 830 Size: 1944 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5422 PvuII CAG'CTG Cuts at: 2353 2353 Size: 5422 SacII CC_GC'GG Cuts at: 2583 2583 Size: 5422 SalI G'TCGA_C Cuts at: 1936 2764 1936 Size: 828 4594 SbfI CC_TGCA'GG Cuts at: 2774 2774 Size: 5422 ScaI AGT'ACT Cuts at: 3670 5382 3670 Size: 1712 3710 SfcI C'TryA_G Cuts at: 826 1045 2770 3905 4586 4777 826 Size: 219 1725 1135 681 191 1471 Fragments arranged by size: 1725 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2602 2602 Size: 5422 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5422 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5422 SmaI CCC'GGG Cuts at: 1872 2749 1872 Size: 877 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3526 4427 4689 820 Size: 197 926 1583 901 262 1553 Fragments arranged by size: 1583 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3213 493 Size: 2720 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5422 SphI G_CATG'C Cuts at: 1132 2780 1132 Size: 1648 3774 SspI AAT'ATT Cuts at: 5 52 3346 5 Size: 47 3294 2081 Fragments arranged by size: 3294 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5422 StyI C'CwwG_G Cuts at: 513 1065 2004 2280 2861 513 Size: 552 939 276 581 3074 Fragments arranged by size: 3074 939 581 552 276 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2636 3449 3466 3619 3804 4249 325 Size: 1255 1056 813 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1056 813 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3668 5380 96 Size: 274 80 33 51 1443 1691 1712 138 Fragments arranged by size: 1712 1691 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2144 2176 2678 2710 5382 5414 2144 Size: 32 502 32 2672 32 2152 Fragments arranged by size: 2672 2152 502 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5422 XbaI T'CTAG_A Cuts at: 2047 2758 2047 Size: 711 4711 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5422 XmaI C'CCGG_G Cuts at: 1870 2747 1870 Size: 877 4545 XmnI GAAnn'nnTTC Cuts at: 3551 3551 Size: 5422 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3230 276 Size: 53 83 186 936 1696 2468 Fragments arranged by size: 2468 1696 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI