################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI AocI Aor51HI AscI Asp718I AspEI AspI AvaI AvaII AviII BbeI BbrPI BbvCI BciVI BcnI BfrI BglI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HpaI HpaII HphI KasI KpnI KspI MamI MfeI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI Sse8387I SspBI StuI SwaI TaqI Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccI 259 GTAGAC GT^[AC][GT]AC Alw44I 773 GTGCAC G^TGCAC AlwNI 785 CAGTCGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 1026 GGGCCC GGGCC^C ApaLI 773 GTGCAC G^TGCAC AseI 1122 ATTAAT AT^TAAT AsnI 1122 ATTAAT AT^TAAT Asp700I 610 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 773 GTGCAC G[AT]GC[AT]^C AvrII 1402 CCTAGG C^CTAGG BanI 1174 GGTGCC G^G[CT][GA]CC BanII 1026 GGGCCC G[GA]GC[CT]^C BcgI 1219 GCAAGAGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1149 TGATCA T^GATCA BglII 1040 AGATCT A^GATCT BlnI 1402 CCTAGG C^CTAGG BpmI 266 CTGGAG CTGGAG(16/14) BsiHKAI 773 GTGCAC G[AT]GC[AT]^C BsmAI 989 GAGAC GTCTC(1/5) BsmFI 1066 GGGAC GGGAC(10/14) BspHI 1016 TCATGA T^CATGA BsrI 1353 CCAGT ACTGG(1/-1) BstXI 1388 CCAAAATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraII 1026 GGGCCCC [GA]G^G[ACGT]CC[CT] DraIII 776 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO109I 1026 GGGCCCC [GA]G^G[ACGT]CC[CT] EcoRI 467 GAATTC G^AATTC FbaI 1149 TGATCA T^GATCA HincII 919 GTCAAC GT[CT]^[GA]AC HindII 919 GTCAAC GT[CT]^[GA]AC HindIII 253 AAGCTT A^AGCTT MaeII 982 ACGT A^CGT NheI 360 GCTAGC G^CTAGC PshAI 1436 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1122 ATTAAT AT^TAAT RcaI 1016 TCATGA T^CATGA SpeI 141 ACTAGT A^CTAGT SphI 228 GCATGC GCATG^C SspI 133 AATATT AAT^ATT TspRI 780 CAGTG CA[GC]TG[ACGT][ACGT]^ XmnI 610 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AciI 110 CCGC CCGC(-3/-1) 579 CCGC BalI 753 TGGCCA TGG^CCA 1385 TGGCCA BamHI 498 GGATCC G^GATCC 1398 GGATCC BbsI 390 GAAGAC GAAGAC(2/6) 592 GAAGAC BbvI 790 GCTGC GCAGC(8/12) 1471 GCTGC BmyI 773 GTGCAC G[AGT]GC[ACT]^C 1026 GGGCCC BpuAI 390 GAAGAC GAAGAC(2/6) 592 GAAGAC BsgI 407 GTGCAG GTGCAG(16/14) 782 GTGCAG BsmI 84 GAATGC GAATGC(1/-1) 450 GAATGC Bsp1286I 773 GTGCAC G[AGT]GC[ACT]^C 1026 GGGCCC BsrDI 510 CATTGC GCAATG(2/0) 53 GCAATG BstAPI 775 GCACTCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 784 GCAGTCGCTGC Cfr13I 1026 GGGCC G^G[ACGT]CC 1027 GGCCC EarI 73 GAAGAG CTCTTC(1/4) 187 GAAGAG EcoT14I 15 CCTTGG C^C[AT][AT]GG 1402 CCTAGG Ksp632I 73 GAAGAG CTCTTC(1/4) 187 GAAGAG MaeIII 739 GTGAC ^GT[ACGT]AC 1356 GTCAC MluNI 753 TGGCCA TGG^CCA 1385 TGGCCA MscI 753 TGGCCA TGG^CCA 1385 TGGCCA PflMI 472 CCATTTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1377 CCAACAAATGG PleI 595 GACTC GAGTC(4/5) 1099 GAGTC Sau96I 1026 GGGCC G^G[ACGT]CC 1027 GGCCC SfaNI 664 GATGC GCATC(5/9) 196 GCATC StyI 15 CCTTGG C^C[AT][AT]GG 1402 CCTAGG TseI 790 GCTGC G^C[AT]GC 1471 GCTGC Tsp45I 739 GTGAC ^GT[GC]AC 1356 GTCAC Van91I 472 CCATTTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1377 CCAACAAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AcsI AfaI AflIII AluI AlwI ApoI BfaI BsaJI BsiYI BslI BspLU11I BssKI BstF5I BstNI BstYI Cac8I DdeI DpnII DraI EaeI EcoRII Fnu4HI FokI HaeIII HinfI ItaI MaeI MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV NspI RsaI Sau3AI ScrFI TfiI Tru9I Tsp509I XhoII