(Circular) (Six-base) MAPSORT of: pFN21AE5637.seq Check: 2106 from: 1 to: 6217 pFN21AE5637 6217 bp With 182 enzymes: SgfI * December 8, 2014 13:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4027 278 Size: 53 83 186 936 2491 2468 Fragments arranged by size: 2491 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2265 3560 1937 Size: 328 1295 4594 Fragments arranged by size: 4594 1295 328 Acc65I G'GTAC_C Cuts at: 3538 3538 Size: 6217 AclI AA'CG_TT Cuts at: 1563 4344 4717 1563 Size: 2781 373 3063 Fragments arranged by size: 3063 2781 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2544 3022 3428 4280 5664 5932 1632 Size: 912 478 406 852 1384 268 1917 Fragments arranged by size: 1917 1384 912 852 478 406 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6020 AflIII A'CryG_T Cuts at: 1203 2354 2997 3339 5116 6093 1203 Size: 1151 643 342 1777 977 1327 Fragments arranged by size: 1777 1327 1151 977 643 342 AgeI A'CCGG_T Cuts at: 5048 5048 Size: 6217 AhdI GACnn_n'nnGTC Cuts at: 4946 4946 Size: 6217 AleI CACnn'nnGTG Cuts at: 1166 3426 1166 Size: 2260 3957 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6217 AlwNI CAG_nnn'CTG Cuts at: 2795 3960 5532 2795 Size: 1165 1572 3480 Fragments arranged by size: 3480 1572 1165 ApaI G_GGCC'C Cuts at: 3035 3035 Size: 6217 ApaLI G'TGCA_C Cuts at: 2778 4274 5430 2778 Size: 1496 1156 3565 Fragments arranged by size: 3565 1496 1156 ApoI r'AATT_y Cuts at: 1493 2472 2537 2551 3526 3752 3904 6013 1493 Size: 979 65 14 975 226 152 2109 1697 Fragments arranged by size: 2109 1697 979 975 226 152 65 14 AseI AT'TA_AT Cuts at: 160 1032 3128 4771 160 Size: 872 2096 1643 1606 Fragments arranged by size: 2096 1643 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 AvaI C'yCGr_G Cuts at: 1870 1943 3542 1870 Size: 73 1599 4545 Fragments arranged by size: 4545 1599 73 AvrII C'CTAG_G Cuts at: 3407 3407 Size: 6217 BamHI G'GATC_C Cuts at: 2503 3403 3547 3914 2503 Size: 900 144 367 4806 Fragments arranged by size: 4806 900 367 144 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3179 3538 4993 618 Size: 325 217 627 1392 359 1455 1842 Fragments arranged by size: 1842 1455 1392 627 359 325 217 BanII G_rGCy'C Cuts at: 1391 3035 3536 1391 Size: 1644 501 4072 Fragments arranged by size: 4072 1644 501 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2402 2604 928 Size: 971 503 202 4541 Fragments arranged by size: 4541 971 503 202 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3213 3247 4408 4442 1903 Size: 34 1276 34 1161 34 3678 Fragments arranged by size: 3678 1276 1161 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4109 5319 4109 Size: 1210 5007 BclI T'GATC_A Cuts at: 1548 3154 1548 Size: 1606 4611 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4828 136 Size: 107 122 71 631 605 3156 1525 Fragments arranged by size: 3156 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3045 6212 3045 Size: 3167 3050 BlpI GC'TnA_GC Cuts at: 3634 3634 Size: 6217 Bme1580I G_kGCm'C Cuts at: 1790 2782 3035 4278 5434 1790 Size: 992 253 1243 1156 2573 Fragments arranged by size: 2573 1243 1156 992 253 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6217 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3948 4906 442 Size: 416 53 83 399 24 2531 958 1753 Fragments arranged by size: 2531 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2369 1056 Size: 1313 4904 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2292 4877 1239 Size: 1053 2585 2579 Fragments arranged by size: 2585 2579 1053 Bpu10I CC'TnA_GC Cuts at: 2703 2703 Size: 6217 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4342 5207 5505 4342 Size: 865 298 5054 Fragments arranged by size: 5054 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4880 882 Size: 617 3381 2219 Fragments arranged by size: 3381 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4008 493 Size: 711 2804 2702 Fragments arranged by size: 2804 2702 711 BsaBI GATnn'nnATC Cuts at: 6211 6211 Size: 6217 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4024 4406 5041 275 Size: 53 83 186 936 2491 382 635 1451 Fragments arranged by size: 2491 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4650 5048 5322 5469 5880 4650 Size: 398 274 147 411 4987 Fragments arranged by size: 4987 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5992 Fragments arranged by size: 5992 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6217 BseYI C'CCAG_C Cuts at: 5420 5916 5420 Size: 496 5721 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2433 2808 1163 Size: 573 697 375 4572 Fragments arranged by size: 4572 697 573 375 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3667 4428 5456 1540 Size: 275 186 1666 761 1028 2301 Fragments arranged by size: 2301 1666 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2782 4278 4363 5434 2782 Size: 1496 85 1071 3565 Fragments arranged by size: 3565 1496 1071 85 BsmI GAATG_Cn' Cuts at: 2095 2461 3728 3821 2095 Size: 366 1267 93 4491 Fragments arranged by size: 4491 1267 366 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2782 3035 3536 4278 4363 5434 1391 Size: 399 992 253 501 742 85 1071 2174 Fragments arranged by size: 2174 1071 992 742 501 399 253 85 BspEI T'CCGG_A Cuts at: 5880 5880 Size: 6217 BspHI T'CATG_A Cuts at: 3021 4106 5836 3021 Size: 1085 1730 3402 Fragments arranged by size: 3402 1730 1085 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3572 844 Size: 2728 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4104 1394 Size: 429 2281 3507 Fragments arranged by size: 3507 2281 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2065 2514 4712 4886 65 Size: 1183 817 449 2198 174 1396 Fragments arranged by size: 2198 1396 1183 817 449 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4861 5048 1700 Size: 110 3051 187 2869 Fragments arranged by size: 3051 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6217 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6217 BssSI C'ACGA_G Cuts at: 4277 5289 4277 Size: 1012 5205 BstAPI GCAn_nnn'nTGC Cuts at: 794 2786 2795 794 Size: 1992 9 4216 Fragments arranged by size: 4216 1992 9 BstBI TT'CG_AA Cuts at: 5021 5021 Size: 6217 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6217 BstXI CCAn_nnnn'nTGG Cuts at: 3400 3400 Size: 6217 BstYI r'GATC_y Cuts at: 1972 2503 3045 3403 3547 3914 4299 4316 5768 Size: 531 542 358 144 367 385 17 1452 Cuts at: 5768 5854 5866 6212 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 542 531 385 367 358 346 144 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4726 Fragments arranged by size: 4726 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5478 Fragments arranged by size: 5478 683 56 BtsI GCAGTG_nn' Cuts at: 3733 4527 4547 3733 Size: 794 20 5403 Fragments arranged by size: 5403 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6217 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6182 DraI TTT'AAA Cuts at: 2245 2542 2713 3521 2245 Size: 297 171 808 4941 Fragments arranged by size: 4941 808 297 171 DraIII CAC_nnn'GTG Cuts at: 2786 2786 Size: 6217 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5224 809 Size: 507 3908 1802 Fragments arranged by size: 3908 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2581 2758 3390 Size: 54 1425 50 275 18 751 177 632 Cuts at: 3390 3664 4553 6182 8 Size: 274 889 1629 43 Fragments arranged by size: 1629 1425 889 751 632 275 274 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3664 1537 Size: 275 1852 4090 Fragments arranged by size: 4090 1852 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2073 2187 4147 1353 Size: 720 114 1960 3423 Fragments arranged by size: 3423 1960 720 114 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4804 5178 5324 387 Size: 1064 138 3215 374 146 1280 Fragments arranged by size: 3215 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2292 2544 3022 3428 4280 4877 5664 Size: 393 660 252 478 406 852 597 787 Cuts at: 5664 5932 1239 Size: 268 1524 Fragments arranged by size: 1524 852 787 660 597 478 406 393 268 252 EcoO109I rG'GnC_Cy Cuts at: 3032 3032 Size: 6217 EcoRI G'AATT_C Cuts at: 2472 3526 3904 2472 Size: 1054 378 4785 Fragments arranged by size: 4785 1054 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2636 2636 Size: 6217 FspI TGC'GCA Cuts at: 4723 4723 Size: 6217 HaeII r_GCGC'y Cuts at: 1678 5364 1678 Size: 3686 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2186 2218 2717 2749 4858 Size: 32 135 32 614 32 499 32 2109 Cuts at: 4858 4890 4932 4964 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2109 614 499 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2926 3561 3807 669 Size: 988 281 988 635 246 3079 Fragments arranged by size: 3079 988 988 635 281 246 HindIII A'AGCT_T Cuts at: 748 2258 748 Size: 1510 4707 HpaI GTT'AAC Cuts at: 3807 3807 Size: 6217 KpnI G_GTAC'C Cuts at: 3542 3542 Size: 6217 MfeI C'AATT_G Cuts at: 3816 3816 Size: 6217 MluI A'CGCG_T Cuts at: 6093 6093 Size: 6217 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2103 2305 2372 2919 5331 5515 1305 Size: 798 202 67 547 2412 184 2007 Fragments arranged by size: 2412 2007 798 547 202 184 67 MscI TGG'CCA Cuts at: 10 64 1489 1832 2760 3392 10 Size: 54 1425 343 928 632 2835 Fragments arranged by size: 2835 1425 928 632 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2841 3026 3426 4175 4534 4693 518 Size: 648 1675 185 400 749 359 159 2042 Fragments arranged by size: 2042 1675 749 648 400 359 185 159 MspA1I CmG'CkG Cuts at: 1606 3633 4310 5458 5703 1606 Size: 2027 677 1148 245 2120 Fragments arranged by size: 2120 2027 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4726 Fragments arranged by size: 4726 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6217 NheI G'CTAG_C Cuts at: 1052 2365 1052 Size: 1313 4904 NotI GC'GGCC_GC Cuts at: 3664 3664 Size: 6217 NspI r_CATG'y Cuts at: 1132 1229 2237 2358 3001 3343 3575 5120 1132 Size: 97 1008 121 643 342 232 1545 2229 Fragments arranged by size: 2229 1545 1008 643 342 232 121 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6217 PciI A'CATG_T Cuts at: 2354 2997 3339 5116 2354 Size: 643 342 1777 3455 Fragments arranged by size: 3455 1777 643 342 PflMI CCAn_nnn'nTGG Cuts at: 1108 2483 3388 1108 Size: 1375 905 3937 Fragments arranged by size: 3937 1375 905 PmeI GTTT'AAAC Cuts at: 3521 3521 Size: 6217 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4286 4318 5799 5831 4286 Size: 32 1481 32 4672 Fragments arranged by size: 4672 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3445 1613 Size: 1832 4385 PsiI TTA'TAA Cuts at: 3787 3787 Size: 6217 PspOMI G'GGCC_C Cuts at: 3031 3031 Size: 6217 PstI C_TGCA'G Cuts at: 830 3569 830 Size: 2739 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6217 SalI G'TCGA_C Cuts at: 1936 3559 1936 Size: 1623 4594 SbfI CC_TGCA'GG Cuts at: 3569 3569 Size: 6217 ScaI AGT'ACT Cuts at: 4465 6177 4465 Size: 1712 4505 SfcI C'TryA_G Cuts at: 826 1045 3565 4700 5381 5572 826 Size: 219 2520 1135 681 191 1471 Fragments arranged by size: 2520 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6217 SmaI CCC'GGG Cuts at: 1872 3544 1872 Size: 1672 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4321 5222 5484 820 Size: 197 926 2378 901 262 1553 Fragments arranged by size: 2378 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4008 493 Size: 3515 2702 SpeI A'CTAG_T Cuts at: 152 2146 152 Size: 1994 4223 SphI G_CATG'C Cuts at: 1132 2237 3575 1132 Size: 1105 1338 3774 Fragments arranged by size: 3774 1338 1105 SspI AAT'ATT Cuts at: 5 52 2140 4141 5 Size: 47 2088 2001 2081 Fragments arranged by size: 2088 2081 2001 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6217 StyI C'CwwG_G Cuts at: 513 1065 2004 2020 3407 3656 513 Size: 552 939 16 1387 249 3074 Fragments arranged by size: 3074 1387 939 552 249 16 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2305 3416 4244 4261 4414 4599 5044 Size: 1255 725 1111 828 17 153 185 445 Cuts at: 5044 325 Size: 1498 Fragments arranged by size: 1498 1255 1111 828 725 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4463 6175 96 Size: 274 80 33 51 1443 2486 1712 138 Fragments arranged by size: 2486 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2791 2823 6177 6209 2791 Size: 32 3354 32 2799 Fragments arranged by size: 3354 2799 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6217 XbaI T'CTAG_A Cuts at: 3553 3553 Size: 6217 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6217 XmaI C'CCGG_G Cuts at: 1870 3542 1870 Size: 1672 4545 XmnI GAAnn'nnTTC Cuts at: 2619 4346 2619 Size: 1727 4490 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4025 276 Size: 53 83 186 936 2491 2468 Fragments arranged by size: 2491 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI