(Linear) (Six-base) MAPSORT of: KIEE5637.seq Check: 400 from: 1 to: 1512 KIEE5637.orf With 182 enzymes: SgfI * December 5, 2014 13:04 .. AccI GT'mk_AC Cuts at: 0 260 1512 Size: 260 1252 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 539 1017 1423 1512 Size: 539 478 406 89 Fragments arranged by size: 539 478 406 89 AflIII A'CryG_T Cuts at: 0 349 992 1334 1512 Size: 349 643 342 178 Fragments arranged by size: 643 349 342 178 AleI CACnn'nnGTG Cuts at: 0 1421 1512 Size: 1421 91 AlwNI CAG_nnn'CTG Cuts at: 0 790 1512 Size: 790 722 ApaI G_GGCC'C Cuts at: 0 1030 1512 Size: 1030 482 ApaLI G'TGCA_C Cuts at: 0 773 1512 Size: 773 739 ApoI r'AATT_y Cuts at: 0 467 532 546 1512 Size: 467 65 14 966 Fragments arranged by size: 966 467 65 14 AseI AT'TA_AT Cuts at: 0 1123 1512 Size: 1123 389 AvrII C'CTAG_G Cuts at: 0 1402 1512 Size: 1402 110 BamHI G'GATC_C Cuts at: 0 498 1398 1512 Size: 498 900 114 Fragments arranged by size: 900 498 114 BanI G'GyrC_C Cuts at: 0 1174 1512 Size: 1174 338 BanII G_rGCy'C Cuts at: 0 1030 1512 Size: 1030 482 BbsI GAAGACnn'nnnn_ Cuts at: 0 397 599 1512 Size: 397 202 913 Fragments arranged by size: 913 397 202 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1208 1242 1512 Size: 1208 34 270 Fragments arranged by size: 1208 270 34 BclI T'GATC_A Cuts at: 0 1149 1512 Size: 1149 363 BglII A'GATC_T Cuts at: 0 1040 1512 Size: 1040 472 Bme1580I G_kGCm'C Cuts at: 0 777 1030 1512 Size: 777 253 482 Fragments arranged by size: 777 482 253 BmtI G_CTAG'C Cuts at: 0 364 1512 Size: 364 1148 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 287 1512 Size: 287 1225 Bpu10I CC'TnA_GC Cuts at: 0 698 1512 Size: 698 814 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 428 803 1512 Size: 428 375 709 Fragments arranged by size: 709 428 375 BsiHKAI G_wGCw'C Cuts at: 0 777 1512 Size: 777 735 BsmI GAATG_Cn' Cuts at: 0 90 456 1512 Size: 90 366 1056 Fragments arranged by size: 1056 366 90 Bsp1286I G_dGCh'C Cuts at: 0 777 1030 1512 Size: 777 253 482 Fragments arranged by size: 777 482 253 BspHI T'CATG_A Cuts at: 0 1016 1512 Size: 1016 496 BsrDI GCAATG_nn' Cuts at: 0 60 509 1512 Size: 60 449 1003 Fragments arranged by size: 1003 449 60 BstAPI GCAn_nnn'nTGC Cuts at: 0 781 790 1512 Size: 781 9 722 Fragments arranged by size: 781 722 9 BstXI CCAn_nnnn'nTGG Cuts at: 0 1395 1512 Size: 1395 117 BstYI r'GATC_y Cuts at: 0 498 1040 1398 1512 Size: 498 542 358 114 Fragments arranged by size: 542 498 358 114 DraI TTT'AAA Cuts at: 0 240 537 708 1512 Size: 240 297 171 804 Fragments arranged by size: 804 297 240 171 DraIII CAC_nnn'GTG Cuts at: 0 781 1512 Size: 781 731 EaeI y'GGCC_r Cuts at: 0 576 753 1385 1512 Size: 576 177 632 127 Fragments arranged by size: 632 576 177 127 EarI CTCTTCn'nnn_ Cuts at: 0 68 182 1512 Size: 68 114 1330 Fragments arranged by size: 1330 114 68 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 287 539 1017 1423 1512 Size: 287 252 478 406 89 Fragments arranged by size: 478 406 287 252 89 EcoO109I rG'GnC_Cy Cuts at: 0 1027 1512 Size: 1027 485 EcoRI G'AATT_C Cuts at: 0 467 1512 Size: 467 1045 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 631 1512 Size: 631 881 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 181 213 712 744 1512 Size: 181 32 499 32 768 Fragments arranged by size: 768 499 181 32 32 HincII GTy'rAC Cuts at: 0 921 1512 Size: 921 591 HindIII A'AGCT_T Cuts at: 0 253 1512 Size: 253 1259 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 98 300 367 914 1512 Size: 98 202 67 547 598 Fragments arranged by size: 598 547 202 98 67 MscI TGG'CCA Cuts at: 0 755 1387 1512 Size: 755 632 125 Fragments arranged by size: 755 632 125 MslI CAynn'nnrTG Cuts at: 0 836 1021 1421 1512 Size: 836 185 400 91 Fragments arranged by size: 836 400 185 91 NheI G'CTAG_C Cuts at: 0 360 1512 Size: 360 1152 NspI r_CATG'y Cuts at: 0 232 353 996 1338 1512 Size: 232 121 643 342 174 Fragments arranged by size: 643 342 232 174 121 PciI A'CATG_T Cuts at: 0 349 992 1334 1512 Size: 349 643 342 178 Fragments arranged by size: 643 349 342 178 PflMI CCAn_nnn'nTGG Cuts at: 0 478 1383 1512 Size: 478 905 129 Fragments arranged by size: 905 478 129 PshAI GACnn'nnGTC Cuts at: 0 1440 1512 Size: 1440 72 PspOMI G'GGCC_C Cuts at: 0 1026 1512 Size: 1026 486 SpeI A'CTAG_T Cuts at: 0 141 1512 Size: 141 1371 SphI G_CATG'C Cuts at: 0 232 1512 Size: 232 1280 SspI AAT'ATT Cuts at: 0 135 1512 Size: 135 1377 StyI C'CwwG_G Cuts at: 0 15 1402 1512 Size: 15 1387 110 Fragments arranged by size: 1387 110 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 300 1411 1512 Size: 300 1111 101 Fragments arranged by size: 1111 300 101 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 786 818 1512 Size: 786 32 694 Fragments arranged by size: 786 694 32 XmnI GAAnn'nnTTC Cuts at: 0 614 1512 Size: 614 898 Enzymes that do cut: AccI AcuI AflIII AleI AlwNI ApaI ApaLI ApoI AseI AvrII BamHI BanI BanII BbsI BcgI BclI BglII Bme1580I BmtI BpmI Bpu10I BsgI BsiHKAI BsmI Bsp1286I BspHI BsrDI BstAPI BstXI BstYI DraI DraIII EaeI EarI Eco57MI EcoO109I EcoRI FalI Hin4I HincII HindIII MmeI MscI MslI NheI NspI PciI PflMI PshAI PspOMI SpeI SphI SspI StyI TaqII TstI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI AscI AsiSI AvaI BaeI BbeI BbvCI BciVI BfrBI BglI BlpI BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DrdI EagI EciI EcoICRI EcoNI EcoRV FseI FspI FspAI HaeII HpaI KasI KpnI MfeI MluI MspA1I NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI PacI PasI PfoI PmeI PmlI PpiI PpuMI PsiI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SrfI StuI SwaI TatI Tth111I XbaI XcmI XhoI XmaI ZraI