Restriction map for
pFN21AE2407
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# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspI AvaI AviII AvrII
BamHI BanI BbeI BcgI BclI
BglI BlnI BlpI Bpu1102I BsaBI
BsaHI BsaWI BseAI BsiWI BsmBI
BsoBI BspEI BssHII Bst1107I BstAPI
BstEII BstPI BstZ17I CelII CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoNI EcoO65I FbaI FseI FspI
HaeII HgaI Hin1I KasI KpnI
MamI MluI MroI NaeI NarI
NcoI NgoMIV NotI PacI PaeR7I
PflFI PinAI PmeI PshBI PvuI
RsrII SbfI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI SwaI Tth111I XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 63 enzymes) #
###################################
AclI 3500 AACGTT AA^CGTT
AflIII 453 ACATGT A^C[GA][CT]GT
Alw44I 2693 GTGCAC G^TGCAC
AlwNI 1583 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2461 CCTCAGG CC^T[ACGT]AGG
ApaLI 2693 GTGCAC G^TGCAC
BalI 3759 TGGCCA TGG^CCA
BanII 2266 GAGCTC G[GA]GC[CT]^C
BbrPI 2528 CACGTG CAC^GTG
BbvCI 2743 CCTCAGC CCTCAGC(-5/-2)
BciVI 2467 GTATCC GTATCC(6/5)
BcnI 1848 CCGGG CC^[GC]GG
BsiEI 2325 CGACCG CG[GA][CT]^CG
BsmI 3345 GAATGC GAATGC(1/-1)
Bsp1407I 2445 TGTACA T^GTACA
BspDI 341 ATCGAT AT^CGAT
BspHI 4200 TCATGA T^CATGA
BspLU11I 453 ACATGT A^CATGT
BspMI 50 ACCTGC ACCTGC(4/8)
BsrBI 3178 GAGCGG CCGCTC(-3/-3)
BsrFI 4548 ACCGGC [GA]^CCGG[CT]
BsrGI 2445 TGTACA T^GTACA
BssSI 659 CTCGTG CACGAG(-5/-1)
BstBI 4410 TTCGAA TT^CGAA
Bsu36I 2461 CCTCAGG CC^T[ACGT]AGG
Cfr10I 4548 ACCGGC [GA]^CCGG[CT]
ClaI 341 ATCGAT AT^CGAT
DraI 2161 TTTAAA TTT^AAA
DraII 4289 AGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 1406 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 44 CCGCGG C^C[GA][CT]GG
Eco81I 2461 CCTCAGG CC^T[ACGT]AGG
EcoO109I 4289 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 1901 GAATTC G^AATTC
FauI 43 CCCGC CCCGC(4/6)
HpaI 457 GTTAAC GTT^AAC
KspI 44 CCGCGG CCGC^GG
MfeI 551 CAATTG C^AATTG
MluNI 3759 TGGCCA TGG^CCA
MscI 3759 TGGCCA TGG^CCA
MunI 551 CAATTG C^AATTG
NciI 1848 CCGGG CC^[GC]GG
NheI 2700 GCTAGC G^CTAGC
NruI 2322 TCGCGA TCG^CGA
NspV 4410 TTCGAA TT^CGAA
PflMI 700 CCACAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2528 CACGTG CAC^GTG
PmlI 2528 CACGTG CAC^GTG
PpuMI 4289 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 2518 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3500 AACGTT AA^CGTT
PstI 1512 CTGCAG CTGCA^G
RcaI 4200 TCATGA T^CATGA
SacI 2266 GAGCTC GAGCT^C
SacII 44 CCGCGG CCGC^GG
SalI 3488 GTCGAC G^TCGAC
SapI 3740 GCTCTTC GCTCTTC(1/4)
ScaI 3085 AGTACT AGT^ACT
SfuI 4410 TTCGAA TT^CGAA
SspBI 2445 TGTACA T^GTACA
SspI 4078 AATATT AAT^ATT
Van91I 700 CCACAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1755 CCATGCTCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 173 GTAGAC GT^[AC][GT]AC
3488 GTCGAC
AflII 1219 CTTAAG C^TTAAG
1927 CTTAAG
AhdI 4161 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4505 GACTCCACGTC
Asp700I 2777 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4172 GAAGGAGTTC
AspEI 4161 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4505 GACTCCACGTC
BfrI 1219 CTTAAG C^TTAAG
1927 CTTAAG
BsaAI 1142 TACGTG [CT]AC^GT[GA]
2528 CACGTG
BsaI 831 GAGACC GGTCTC(1/5)
3528 GAGACC
BsiYI 700 CCACAAGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
3685 CCCAAACGAGG
BslI 700 CCACAAGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
3685 CCCAAACGAGG
BsmFI 4016 GTCCC GGGAC(10/14)
4291 GTCCC
CfoI 297 GCGC GCG^C
3349 GCGC
EaeI 3759 TGGCCA [CT]^GGCC[GA]
4182 TGGCCG
Eam1105I 4161 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4505 GACTCCACGTC
EcoRV 319 GATATC GAT^ATC
1619 GATATC
EcoT22I 1307 ATGCAT ATGCA^T
2789 ATGCAT
HhaI 297 GCGC GCG^C
3349 GCGC
HinP1I 297 GCGC G^CGC
3349 GCGC
HincII 457 GTTAAC GT[CT]^[GA]AC
3488 GTCGAC
HindII 457 GTTAAC GT[CT]^[GA]AC
3488 GTCGAC
HindIII 673 AAGCTT A^AGCTT
4498 AAGCTT
NdeI 329 CATATG CA^TATG
2792 CATATG
NlaIV 2890 GGAGCC GG[ACGT]^[ACGT]CC
4290 GGTCCC
NsiI 1307 ATGCAT ATGCA^T
2789 ATGCAT
XmnI 2777 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4172 GAAGGAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaII BbsI
BbvI BfaI BglII BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsmAI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NdeII NlaIII NspI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI XhoII