Restriction map for
pFN21AE2407
(Linear) (Six-base) MAPSORT of: KIEE2407.seq Check: 5563 from: 1 to: 4572
KIEE2407.orf
With 182 enzymes: SgfI *
May 11, 2012 13:46 ..
AccI GT'mk_AC
Cuts at: 0 174 3489 4572
Size: 174 3315 1083
Fragments arranged by size:
3315 1083 174
AclI AA'CG_TT
Cuts at: 0 3501 4572
Size: 3501 1071
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 703 789 1478 1565 2117 2423 2735
Size: 217 486 86 689 87 552 306 312
Cuts at: 2735 3482 4017 4572
Size: 747 535 555
Fragments arranged by size:
747 689 555 552 535 486 312 306
217 87 86
AflII C'TTAA_G
Cuts at: 0 1219 1927 4572
Size: 1219 708 2645
Fragments arranged by size:
2645 1219 708
AflIII A'CryG_T
Cuts at: 0 453 4572
Size: 453 4119
AhdI GACnn_n'nnGTC
Cuts at: 0 4166 4510 4572
Size: 4166 344 62
Fragments arranged by size:
4166 344 62
AleI CACnn'nnGTG
Cuts at: 0 1714 4572
Size: 1714 2858
AlwNI CAG_nnn'CTG
Cuts at: 0 1588 4572
Size: 1588 2984
ApaLI G'TGCA_C
Cuts at: 0 2693 4572
Size: 2693 1879
ApoI r'AATT_y
Cuts at: 0 308 1901 1989 2167 2722 4572
Size: 308 1593 88 178 555 1850
Fragments arranged by size:
1850 1593 555 308 178 88
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 172 205 4572
Size: 172 33 4367
Fragments arranged by size:
4367 172 33
BanII G_rGCy'C
Cuts at: 0 2270 4572
Size: 2270 2302
BbsI GAAGACnn'nnnn_
Cuts at: 0 2411 3008 3516 3566 3702 4572
Size: 2411 597 508 50 136 870
Fragments arranged by size:
2411 870 597 508 136 50
BbvCI CC'TCA_GC
Cuts at: 0 2744 4572
Size: 2744 1828
BciVI GTATCCnnnnn_n'
Cuts at: 0 2478 4572
Size: 2478 2094
BfrBI ATG'CAT
Cuts at: 0 1309 2791 4572
Size: 1309 1482 1781
Fragments arranged by size:
1781 1482 1309
BglII A'GATC_T
Cuts at: 0 95 2779 4069 4572
Size: 95 2684 1290 503
Fragments arranged by size:
2684 1290 503 95
Bme1580I G_kGCm'C
Cuts at: 0 2697 4572
Size: 2697 1875
BmgBI CAC'GTC
Cuts at: 0 4512 4572
Size: 4512 60
BmrI ACTGGGnnnn_n'
Cuts at: 0 1679 4174 4514 4572
Size: 1679 2495 340 58
Fragments arranged by size:
2495 1679 340 58
BmtI G_CTAG'C
Cuts at: 0 2704 4572
Size: 2704 1868
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3275 3277 4572
Size: 3275 2 1295
Fragments arranged by size:
3275 1295 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2501 2940 4127 4572
Size: 2501 439 1187 445
Fragments arranged by size:
2501 1187 445 439
Bpu10I CC'TnA_GC
Cuts at: 0 2744 4572
Size: 2744 1828
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 2438 3433 3644 3735 4572
Size: 223 2215 995 211 91 837
Fragments arranged by size:
2215 995 837 223 211 91
BsaI GGTCTCn'nnnn_
Cuts at: 0 825 3522 4572
Size: 825 2697 1050
Fragments arranged by size:
2697 1050 825
BsaAI yAC'GTr
Cuts at: 0 1144 2530 4572
Size: 1144 1386 2042
Fragments arranged by size:
2042 1386 1144
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3063 3093 3613 3643 4082 4112 4572
Size: 3063 30 520 30 439 30 460
Fragments arranged by size:
3063 520 460 439 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1646 3507 3634 3857 4208 4572
Size: 1646 1861 127 223 351 364
Fragments arranged by size:
1861 1646 364 351 223 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1527 1552 2563 2834 4572
Size: 1527 25 1011 271 1738
Fragments arranged by size:
1738 1527 1011 271 25
BsiEI CG_ry'CG
Cuts at: 0 2328 4572
Size: 2328 2244
BsiHKAI G_wGCw'C
Cuts at: 0 2270 2697 3808 4572
Size: 2270 427 1111 764
Fragments arranged by size:
2270 1111 764 427
BsmI GAATG_Cn'
Cuts at: 0 3351 4572
Size: 3351 1221
Bsp1286I G_dGCh'C
Cuts at: 0 2270 2697 3808 4572
Size: 2270 427 1111 764
Fragments arranged by size:
2270 1111 764 427
BspHI T'CATG_A
Cuts at: 0 4200 4572
Size: 4200 372
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 59 4572
Size: 59 4513
BsrBI CCG'CTC
Cuts at: 0 3180 4572
Size: 3180 1392
BsrDI GCAATG_nn'
Cuts at: 0 3152 4040 4322 4572
Size: 3152 888 282 250
Fragments arranged by size:
3152 888 282 250
BsrFI r'CCGG_y
Cuts at: 0 4548 4572
Size: 4548 24
BsrGI T'GTAC_A
Cuts at: 0 2445 4572
Size: 2445 2127
BssSI C'ACGA_G
Cuts at: 0 659 4572
Size: 659 3913
BstBI TT'CG_AA
Cuts at: 0 4411 4572
Size: 4411 161
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2576 3922 4527 4572
Size: 2576 1346 605 45
Fragments arranged by size:
2576 1346 605 45
BstYI r'GATC_y
Cuts at: 0 95 1530 2457 2779 4069 4572
Size: 95 1435 927 322 1290 503
Fragments arranged by size:
1435 1290 927 503 322 95
Bsu36I CC'TnA_GG
Cuts at: 0 2462 4572
Size: 2462 2110
BtgI C'CryG_G
Cuts at: 0 44 4572
Size: 44 4528
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3392 3611 4136 4572
Size: 3392 219 525 436
Fragments arranged by size:
3392 525 436 219
BtsI GCAGTG_nn'
Cuts at: 0 403 3132 3928 4572
Size: 403 2729 796 644
Fragments arranged by size:
2729 796 644 403
ClaI AT'CG_AT
Cuts at: 0 342 4572
Size: 342 4230
DraI TTT'AAA
Cuts at: 0 2163 4572
Size: 2163 2409
DraIII CAC_nnn'GTG
Cuts at: 0 1411 4572
Size: 1411 3161
EaeI y'GGCC_r
Cuts at: 0 3759 4182 4572
Size: 3759 423 390
Fragments arranged by size:
3759 423 390
EarI CTCTTCn'nnn_
Cuts at: 0 819 1067 1460 2375 2711 3005 3620 3659
Size: 819 248 393 915 336 294 615 39
Cuts at: 3659 3747 4572
Size: 88 825
Fragments arranged by size:
915 825 819 615 393 336 294 248
88 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 91 4572
Size: 91 4481
EcoICRI GAG'CTC
Cuts at: 0 2268 4572
Size: 2268 2304
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 703 789 1478 1565 2117 2423 2501
Size: 217 486 86 689 87 552 306 78
Cuts at: 2501 2735 2940 3482 4017 4127 4572
Size: 234 205 542 535 110 445
Fragments arranged by size:
689 552 542 535 486 445 306 234
217 205 110 87 86 78
EcoO109I rG'GnC_Cy
Cuts at: 0 4290 4572
Size: 4290 282
EcoRI G'AATT_C
Cuts at: 0 1901 4572
Size: 1901 2671
EcoRV GAT'ATC
Cuts at: 0 321 1621 4572
Size: 321 1300 2951
Fragments arranged by size:
2951 1300 321
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 539 1123 1942 3073 3217 3310 3803 4572
Size: 539 584 819 1131 144 93 493 769
Fragments arranged by size:
1131 819 769 584 539 493 144 93
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 335 367 1556 1582 1588 1614 2101 2133
Size: 335 32 1189 26 6 26 487 32
Cuts at: 2133 3431 3463 4096 4128 4152 4184 4496 4528
Size: 1298 32 633 32 24 32 312 32
Cuts at: 4528 4572
Size: 44
Fragments arranged by size:
1298 1189 633 487 335 312 44 32
32 32 32 32 32 26 26 24
6
HincII GTy'rAC
Cuts at: 0 459 3490 4572
Size: 459 3031 1082
Fragments arranged by size:
3031 1082 459
HindIII A'AGCT_T
Cuts at: 0 673 4498 4572
Size: 673 3825 74
Fragments arranged by size:
3825 673 74
HpaI GTT'AAC
Cuts at: 0 459 4572
Size: 459 4113
MfeI C'AATT_G
Cuts at: 0 551 4572
Size: 551 4021
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2534 3229 4572
Size: 2534 695 1343
Fragments arranged by size:
2534 1343 695
MscI TGG'CCA
Cuts at: 0 3761 4572
Size: 3761 811
MslI CAynn'nnrTG
Cuts at: 0 110 434 1714 3920 4572
Size: 110 324 1280 2206 652
Fragments arranged by size:
2206 1280 652 324 110
MspA1I CmG'CkG
Cuts at: 0 6 46 1894 2547 4256 4572
Size: 6 40 1848 653 1709 316
Fragments arranged by size:
1848 1709 653 316 40 6
NdeI CA'TA_TG
Cuts at: 0 330 2793 4572
Size: 330 2463 1779
Fragments arranged by size:
2463 1779 330
NheI G'CTAG_C
Cuts at: 0 2700 4572
Size: 2700 1872
NruI TCG'CGA
Cuts at: 0 2324 4572
Size: 2324 2248
NsiI A_TGCA'T
Cuts at: 0 1311 2793 4572
Size: 1311 1482 1779
Fragments arranged by size:
1779 1482 1311
NspI r_CATG'y
Cuts at: 0 457 2334 2412 4572
Size: 457 1877 78 2160
Fragments arranged by size:
2160 1877 457 78
PciI A'CATG_T
Cuts at: 0 453 4572
Size: 453 4119
PflMI CCAn_nnn'nTGG
Cuts at: 0 706 4572
Size: 706 3866
PfoI T'CCnGG_A
Cuts at: 0 1847 2364 4572
Size: 1847 517 2208
Fragments arranged by size:
2208 1847 517
PmlI CAC'GTG
Cuts at: 0 2530 4572
Size: 2530 2042
PpuMI rG'GwC_Cy
Cuts at: 0 4290 4572
Size: 4290 282
PshAI GACnn'nnGTC
Cuts at: 0 2522 4572
Size: 2522 2050
PsiI TTA'TAA
Cuts at: 0 3230 4572
Size: 3230 1342
PstI C_TGCA'G
Cuts at: 0 1516 4572
Size: 1516 3056
PvuII CAG'CTG
Cuts at: 0 1894 2547 4256 4572
Size: 1894 653 1709 316
Fragments arranged by size:
1894 1709 653 316
SacI G_AGCT'C
Cuts at: 0 2270 4572
Size: 2270 2302
SacII CC_GC'GG
Cuts at: 0 47 4572
Size: 47 4525
SalI G'TCGA_C
Cuts at: 0 3488 4572
Size: 3488 1084
SapI GCTCTTCn'nnn_
Cuts at: 0 3747 4572
Size: 3747 825
ScaI AGT'ACT
Cuts at: 0 3087 4572
Size: 3087 1485
SfcI C'TryA_G
Cuts at: 0 1512 1646 3872 4572
Size: 1512 134 2226 700
Fragments arranged by size:
2226 1512 700 134
SmlI C'TyrA_G
Cuts at: 0 202 1219 1927 2453 3448 3659 3750 4572
Size: 202 1017 708 526 995 211 91 822
Fragments arranged by size:
1017 995 822 708 526 211 202 91
SspI AAT'ATT
Cuts at: 0 4080 4572
Size: 4080 492
StyI C'CwwG_G
Cuts at: 0 2315 2520 2877 3308 3536 4572
Size: 2315 205 357 431 228 1036
Fragments arranged by size:
2315 1036 431 357 228 205
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 807 3825 4533 4572
Size: 807 3018 708 39
Fragments arranged by size:
3018 807 708 39
TatI w'GTAC_w
Cuts at: 0 2445 3085 3106 4572
Size: 2445 640 21 1466
Fragments arranged by size:
2445 1466 640 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 369 401 4572
Size: 369 32 4171
Fragments arranged by size:
4171 369 32
XbaI T'CTAG_A
Cuts at: 0 2632 3505 3520 4572
Size: 2632 873 15 1052
Fragments arranged by size:
2632 1052 873 15
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1762 4572
Size: 1762 2810
XmnI GAAnn'nnTTC
Cuts at: 0 2781 4176 4572
Size: 2781 1395 396
Fragments arranged by size:
2781 1395 396
Enzymes that do cut:
AccI AclI AcuI AflII AflIII AhdI AleI AlwNI
ApaLI ApoI BaeI BanII BbsI BbvCI BciVI BfrBI
BglII Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaXI BseRI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI DraI DraIII EaeI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI Hin4I HincII HindIII HpaI
MfeI MmeI MscI MslI MspA1I NdeI NheI NruI
NsiI NspI PciI PflMI PfoI PmlI PpuMI PshAI
PsiI PstI PvuII SacI SacII SalI SapI ScaI
SfcI SmlI SspI StyI TaqII TatI TstI XbaI
XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AgeI AlfI AloI ApaI
AscI AseI AsiSI AvaI AvrII BamHI BanI BbeI
BcgI BclI BglI BlpI BsaBI BsaHI BsaWI BseYI
BsiWI BsmBI BspEI BssHII BstAPI BstEII BstZ17I CspCI
DrdI EagI EcoNI FseI FspI FspAI HaeII KasI
KpnI MluI NaeI NarI NcoI NgoMIV NotI PacI
PasI PmeI PpiI PspOMI PspXI PsrI PvuI RsrII
SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StuI SwaI Tth111I XhoI
XmaI ZraI