################################## # NO CUT ENZYMES ( 212 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI AluI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BbvI BcgI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI Fnu4HI FseI FspI HapII HgaI Hin1I HincII HindII HindIII HinfI HpaI HpaII HphI ItaI KasI KpnI Ksp632I KspI MaeI MamI MboI MfeI MflI MluI MluNI MnlI MroI MscI MseI MslI MspA1I MspI MunI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I TseI TspRI Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 25 enzymes) # ################################### AccII 20 CGCG CG^CG AlwNI 55 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG BanII 88 GAGCCC G[GA]GC[CT]^C BbsI 160 GTCTTC GAAGAC(2/6) BciVI 154 GGATAC GTATCC(6/5) BmyI 88 GAGCCC G[AGT]GC[ACT]^C BpmI 85 CTGGAG CTGGAG(16/14) BpuAI 160 GTCTTC GAAGAC(2/6) Bsp1286I 88 GAGCCC G[AGT]GC[ACT]^C BspMI 59 ACCTGC ACCTGC(4/8) BsrBI 97 GAGCGG CCGCTC(-3/-3) BsrI 54 CCAGT ACTGG(1/-1) BstUI 20 CGCG CG^CG Cfr13I 23 GGGCC G^G[ACGT]CC HaeIII 24 GGCC GG^CC MaeII 158 ACGT A^CGT MaeIII 67 GTCAC ^GT[ACGT]AC MboII 161 TCTTC GAAGA(8/7) MvnI 20 CGCG CG^CG NlaIV 87 GGAGCC GG[ACGT]^[ACGT]CC NspI 80 ACATGC [GA]CATG^[CT] Sau96I 23 GGGCC G^G[ACGT]CC Tsp45I 67 GTCAC ^GT[GC]AC Tsp509I 112 AATT ^AATT XcmI 128 CCATTGTGGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### AfaI 57 GTAC GT^AC 208 GTAC BsaJI 91 CCCTGG C^C[ACGT][ACGT]GG 92 CCTGGG BsiYI 92 CCTGGGAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 128 CCATTGTGGGG BslI 92 CCTGGGAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 128 CCATTGTGGGG BstF5I 8 GGATG GGATG(2/0) 137 GGATG Cac8I 15 GCTGGC GC[ACGT]^[ACGT]GC 21 GCGGGC FauI 4 GCGGG CCCGC(4/6) 21 GCGGG FokI 8 GGATG GGATG(9/13) 137 GGATG HaeII 12 GGCGCT [GA]GCGC^[CT] 72 GGCGCT RsaI 57 GTAC GT^AC 208 GTAC ######################################### # 3 OR MORE CUT ENZYMES ( 11 enzymes) # ######################################### AciI BssKI BstNI CfoI EcoRII HhaI HinP1I MvaI MwoI NlaIII ScrFI