(Circular) (Six-base) MAPSORT of: pFN21AE2119.seq Check: 3228 from: 1 to: 7441 pFN21AE2119 7441 bp With 182 enzymes: SgfI * March 28, 2012 14:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5251 278 Size: 53 83 186 936 3715 2468 Fragments arranged by size: 3715 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2259 4784 1937 Size: 322 2525 4594 Fragments arranged by size: 4594 2525 322 Acc65I G'GTAC_C Cuts at: 4238 4762 4238 Size: 524 6917 AclI AA'CG_TT Cuts at: 1563 2622 2935 5568 5941 1563 Size: 1059 313 2633 373 3063 Fragments arranged by size: 3063 2633 1059 373 313 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2330 2897 3426 4716 5504 6888 7156 1632 Size: 698 567 529 1290 788 1384 268 1917 Fragments arranged by size: 1917 1384 1290 788 698 567 529 268 AfeI AGC'GCT Cuts at: 4484 4577 4484 Size: 93 7348 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7244 AflIII A'CryG_T Cuts at: 1203 4299 6340 7317 1203 Size: 3096 2041 977 1327 Fragments arranged by size: 3096 2041 1327 977 AgeI A'CCGG_T Cuts at: 6272 6272 Size: 7441 AhdI GACnn_n'nnGTC Cuts at: 4303 6170 4303 Size: 1867 5574 AleI CACnn'nnGTG Cuts at: 1166 3924 1166 Size: 2758 4683 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7441 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3178 3210 3178 Size: 32 7409 AlwNI CAG_nnn'CTG Cuts at: 3014 4636 5184 6756 3014 Size: 1622 548 1572 3699 Fragments arranged by size: 3699 1622 1572 548 ApaI G_GGCC'C Cuts at: 2103 2109 2103 Size: 6 7435 ApaLI G'TGCA_C Cuts at: 2940 5498 6654 2940 Size: 2558 1156 3727 Fragments arranged by size: 3727 2558 1156 ApoI r'AATT_y Cuts at: 1493 2746 3399 3414 3934 4380 4750 4976 5128 Size: 1253 653 15 520 446 370 226 152 Cuts at: 5128 7237 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1253 653 520 446 370 226 152 15 AseI AT'TA_AT Cuts at: 160 1032 2982 5995 160 Size: 872 1950 3013 1606 Fragments arranged by size: 3013 1950 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7441 AvaI C'yCGr_G Cuts at: 1870 1943 2102 4766 1870 Size: 73 159 2664 4545 Fragments arranged by size: 4545 2664 159 73 BamHI G'GATC_C Cuts at: 4771 5138 4771 Size: 367 7074 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4089 4238 4762 6217 618 Size: 325 217 627 2302 149 524 1455 1842 Fragments arranged by size: 2302 1842 1455 627 524 325 217 149 BanII G_rGCy'C Cuts at: 1391 2103 2109 2473 2694 3072 3647 4760 1391 Size: 712 6 364 221 378 575 1113 4072 Fragments arranged by size: 4072 1113 712 575 378 364 221 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2430 4371 928 Size: 971 531 1941 3998 Fragments arranged by size: 3998 1941 971 531 BbvCI CC'TCA_GC Cuts at: 4150 4150 Size: 7441 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5632 5666 1903 Size: 34 3695 34 3678 Fragments arranged by size: 3695 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4413 5333 6543 4413 Size: 920 1210 5311 Fragments arranged by size: 5311 1210 920 BclI T'GATC_A Cuts at: 1548 2485 4656 1548 Size: 937 2171 4333 Fragments arranged by size: 4333 2171 937 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2072 4338 6052 Size: 107 122 71 631 605 400 2266 1714 Cuts at: 6052 136 Size: 1525 Fragments arranged by size: 2266 1714 1525 631 605 400 122 107 71 BglII A'GATC_T Cuts at: 7436 7436 Size: 7441 BlpI GC'TnA_GC Cuts at: 2996 4858 2996 Size: 1862 5579 Bme1580I G_kGCm'C Cuts at: 1790 2103 2109 2683 2944 5502 6658 1790 Size: 313 6 574 261 2558 1156 2573 Fragments arranged by size: 2573 2558 1156 574 313 261 6 BmgBI CAC'GTC Cuts at: 1318 2233 1318 Size: 915 6526 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2028 2643 3779 Size: 416 53 83 399 24 611 615 1136 Cuts at: 3779 5172 6130 442 Size: 1393 958 1753 Fragments arranged by size: 1753 1393 1136 958 615 611 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4148 4674 1056 Size: 3092 526 3823 Fragments arranged by size: 3823 3092 526 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2031 2031 Size: 7441 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2457 6101 1239 Size: 1218 3644 2579 Fragments arranged by size: 3644 2579 1218 Bpu10I CC'TnA_GC Cuts at: 2174 4150 4334 2174 Size: 1976 184 5281 Fragments arranged by size: 5281 1976 184 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4525 5566 6431 6729 4525 Size: 1041 865 298 5237 Fragments arranged by size: 5237 1041 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6104 882 Size: 617 4605 2219 Fragments arranged by size: 4605 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5232 493 Size: 711 4028 2702 Fragments arranged by size: 4028 2702 711 BsaBI GATnn'nnATC Cuts at: 3608 7435 3608 Size: 3827 3614 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3177 3956 5248 5630 Size: 53 83 186 936 1644 779 1292 382 Cuts at: 5630 6265 275 Size: 635 1451 Fragments arranged by size: 1644 1451 1292 936 779 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2017 4221 5874 6272 6546 6693 7104 2017 Size: 2204 1653 398 274 147 411 2354 Fragments arranged by size: 2354 2204 1653 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3507 3537 3932 3962 1178 Size: 30 165 30 2104 30 395 30 4657 Fragments arranged by size: 4657 2104 395 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3637 1186 Size: 2451 4990 BseYI C'CCAG_C Cuts at: 6644 7140 6644 Size: 496 6945 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3254 3600 1163 Size: 573 1518 346 5004 Fragments arranged by size: 5004 1518 573 346 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2030 2076 2142 4891 5652 6680 Size: 275 186 29 46 66 2749 761 1028 Cuts at: 6680 1540 Size: 2301 Fragments arranged by size: 2749 2301 1028 761 275 186 66 46 29 BsiHKAI G_wGCw'C Cuts at: 2473 2944 4047 5502 5587 6658 2473 Size: 471 1103 1455 85 1071 3256 Fragments arranged by size: 3256 1455 1103 1071 471 85 BsmI GAATG_Cn' Cuts at: 4393 4565 4952 5045 4393 Size: 172 387 93 6789 Fragments arranged by size: 6789 387 172 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3947 3947 Size: 7441 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2103 2109 2473 2683 2694 2944 3072 Size: 399 313 6 364 210 11 250 128 Cuts at: 3072 3647 4047 4760 5502 5587 6658 1391 Size: 575 400 713 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 713 575 400 399 364 313 250 210 128 85 11 6 BspEI T'CCGG_A Cuts at: 4221 7104 4221 Size: 2883 4558 BspHI T'CATG_A Cuts at: 3222 3273 4432 5330 7060 3222 Size: 51 1159 898 1730 3603 Fragments arranged by size: 3603 1730 1159 898 51 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3375 4023 4796 844 Size: 2531 648 773 3489 Fragments arranged by size: 3489 2531 773 648 BsrBI CCG'CTC Cuts at: 1394 1823 5328 1394 Size: 429 3505 3507 Fragments arranged by size: 3507 3505 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3099 3123 5936 6110 65 Size: 1183 1851 24 2813 174 1396 Fragments arranged by size: 2813 1851 1396 1183 174 24 BsrFI r'CCGG_y Cuts at: 1700 1810 2185 6085 6272 1700 Size: 110 375 3900 187 2869 Fragments arranged by size: 3900 2869 375 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7441 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7441 BssSI C'ACGA_G Cuts at: 3170 4076 5501 6513 3170 Size: 906 1425 1012 4098 Fragments arranged by size: 4098 1425 1012 906 BstAPI GCAn_nnn'nTGC Cuts at: 794 2899 2913 4636 794 Size: 2105 14 1723 3599 Fragments arranged by size: 3599 2105 1723 14 BstBI TT'CG_AA Cuts at: 3480 6245 3480 Size: 2765 4676 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7441 BstXI CCAn_nnnn'nTGG Cuts at: 4416 4416 Size: 7441 BstYI r'GATC_y Cuts at: 1972 2304 3154 4771 5138 5523 5540 6992 7078 Size: 332 850 1617 367 385 17 1452 86 Cuts at: 7078 7090 7436 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1617 1452 850 385 367 346 332 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4167 4260 4268 4604 4167 Size: 93 8 336 7004 Fragments arranged by size: 7004 336 93 8 BtgI C'CryG_G Cuts at: 513 1065 2049 3651 3991 513 Size: 552 984 1602 340 3963 Fragments arranged by size: 3963 1602 984 552 340 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6702 Fragments arranged by size: 6702 683 56 BtsI GCAGTG_nn' Cuts at: 2909 4957 5751 5771 2909 Size: 2048 794 20 4579 Fragments arranged by size: 4579 2048 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7441 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7406 DraI TTT'AAA Cuts at: 2575 4102 4745 2575 Size: 1527 643 5271 Fragments arranged by size: 5271 1527 643 DraIII CAC_nnn'GTG Cuts at: 2853 2853 Size: 7441 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6448 809 Size: 507 5132 1802 Fragments arranged by size: 5132 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2027 2073 2361 Size: 54 1425 50 275 18 197 46 288 Cuts at: 2361 3588 4888 5777 7406 8 Size: 1227 1300 889 1629 43 Fragments arranged by size: 1629 1425 1300 1227 889 288 275 197 54 50 46 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2027 2073 4888 1537 Size: 275 215 46 2815 4090 Fragments arranged by size: 4090 2815 275 215 46 EarI CTCTTCn'nnn_ Cuts at: 1353 2732 3248 3817 5371 1353 Size: 1379 516 569 1554 3423 Fragments arranged by size: 3423 1554 1379 569 516 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2401 2620 6028 6402 6548 387 Size: 1064 138 812 219 3408 374 146 1280 Fragments arranged by size: 3408 1280 1064 812 374 219 146 138 EcoICRI GAG'CTC Cuts at: 2471 2471 Size: 7441 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2330 2457 2897 3426 4716 5504 6101 Size: 393 698 127 440 529 1290 788 597 Cuts at: 6101 6888 7156 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1290 788 787 698 597 529 440 393 268 127 EcoO109I rG'GnC_Cy Cuts at: 4264 4264 Size: 7441 EcoRI G'AATT_C Cuts at: 4750 5128 4750 Size: 378 7063 EcoRV GAT'ATC Cuts at: 2371 3682 2371 Size: 1311 6130 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2402 2402 Size: 7441 FspI TGC'GCA Cuts at: 5947 5947 Size: 7441 HaeII r_GCGC'y Cuts at: 1678 2013 4486 4579 6588 1678 Size: 335 2473 93 2009 2531 Fragments arranged by size: 2531 2473 2009 335 93 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2315 2347 2726 2758 3139 Size: 32 135 32 743 32 379 32 381 Cuts at: 3139 3171 3276 3308 4289 4321 6082 6114 6156 Size: 32 105 32 981 32 1761 32 42 Cuts at: 6156 6188 1373 Size: 32 2626 Fragments arranged by size: 2626 1761 981 743 381 379 135 105 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2260 4785 5031 669 Size: 988 281 322 2525 246 3079 Fragments arranged by size: 3079 2525 988 322 281 246 HindIII A'AGCT_T Cuts at: 748 2870 748 Size: 2122 5319 HpaI GTT'AAC Cuts at: 5031 5031 Size: 7441 KpnI G_GTAC'C Cuts at: 4242 4766 4242 Size: 524 6917 MfeI C'AATT_G Cuts at: 2587 5040 2587 Size: 2453 4988 MluI A'CGCG_T Cuts at: 7317 7317 Size: 7441 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3887 6555 6739 1305 Size: 2582 2668 184 2007 Fragments arranged by size: 2668 2582 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3590 10 Size: 54 1425 343 1758 3861 Fragments arranged by size: 3861 1758 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3272 3924 4593 5399 5758 5917 518 Size: 648 2106 652 669 806 359 159 2042 Fragments arranged by size: 2106 2042 806 669 652 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2041 2063 2072 2350 2595 4154 4857 5534 Size: 435 22 9 278 245 1559 703 677 Cuts at: 5534 6682 6927 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1559 1148 703 677 435 278 245 245 22 9 NcoI C'CATG_G Cuts at: 513 1065 3991 513 Size: 552 2926 3963 Fragments arranged by size: 3963 2926 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7441 NheI G'CTAG_C Cuts at: 1052 4144 4670 1052 Size: 3092 526 3823 Fragments arranged by size: 3823 3092 526 NotI GC'GGCC_GC Cuts at: 2027 2073 4888 2027 Size: 46 2815 4580 Fragments arranged by size: 4580 2815 46 NspI r_CATG'y Cuts at: 1132 1229 3620 4003 4303 4799 6344 1132 Size: 97 2391 383 300 496 1545 2229 Fragments arranged by size: 2391 2229 1545 496 383 300 97 PasI CC'CwG_GG Cuts at: 1348 2090 3002 1348 Size: 742 912 5787 Fragments arranged by size: 5787 912 742 PciI A'CATG_T Cuts at: 4299 6340 4299 Size: 2041 5400 PflMI CCAn_nnn'nTGG Cuts at: 1108 3597 1108 Size: 2489 4952 PfoI T'CCnGG_A Cuts at: 2373 2373 Size: 7441 PmeI GTTT'AAAC Cuts at: 4745 4745 Size: 7441 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5510 5542 7023 7055 5510 Size: 32 1481 32 5896 Fragments arranged by size: 5896 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4264 4264 Size: 7441 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7441 PsiI TTA'TAA Cuts at: 5011 5011 Size: 7441 PspOMI G'GGCC_C Cuts at: 2099 2105 2099 Size: 6 7435 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4265 4297 4265 Size: 32 7409 PstI C_TGCA'G Cuts at: 830 2166 2202 4641 4793 830 Size: 1336 36 2439 152 3478 Fragments arranged by size: 3478 2439 1336 152 36 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7441 PvuII CAG'CTG Cuts at: 2063 2350 4154 2063 Size: 287 1804 5350 Fragments arranged by size: 5350 1804 287 SacI G_AGCT'C Cuts at: 2473 2473 Size: 7441 SalI G'TCGA_C Cuts at: 1936 2258 4783 1936 Size: 322 2525 4594 Fragments arranged by size: 4594 2525 322 SbfI CC_TGCA'GG Cuts at: 4793 4793 Size: 7441 ScaI AGT'ACT Cuts at: 2861 3768 5689 7401 2861 Size: 907 1921 1712 2901 Fragments arranged by size: 2901 1921 1712 907 SexAI A'CCwGG_T Cuts at: 4535 4535 Size: 7441 SfcI C'TryA_G Cuts at: 826 1045 2162 2198 2218 3905 4486 4520 4637 Size: 219 1117 36 20 1687 581 34 117 Cuts at: 4637 4789 5924 6605 6796 826 Size: 152 1135 681 191 1471 Fragments arranged by size: 1687 1471 1135 1117 681 581 219 191 152 117 36 34 20 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7441 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7441 SmaI CCC'GGG Cuts at: 1872 2104 4768 1872 Size: 232 2664 4545 Fragments arranged by size: 4545 2664 232 SmlI C'TyrA_G Cuts at: 820 1017 1943 4504 5545 6446 6708 820 Size: 197 926 2561 1041 901 262 1553 Fragments arranged by size: 2561 1553 1041 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5232 493 Size: 4739 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7441 SphI G_CATG'C Cuts at: 1132 3620 4799 1132 Size: 2488 1179 3774 Fragments arranged by size: 3774 2488 1179 SrfI GCCC'GGGC Cuts at: 2104 2104 Size: 7441 SspI AAT'ATT Cuts at: 5 52 5365 5 Size: 47 5313 2081 Fragments arranged by size: 5313 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7441 StyI C'CwwG_G Cuts at: 513 1065 2965 3639 3991 4514 4880 513 Size: 552 1900 674 352 523 366 3074 Fragments arranged by size: 3074 1900 674 552 523 366 352 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4601 5468 5485 5638 5823 6268 325 Size: 1255 3021 867 17 153 185 445 1498 Fragments arranged by size: 3021 1498 1255 867 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2859 3766 5687 Size: 274 80 33 51 1443 882 907 1921 Cuts at: 5687 7399 96 Size: 1712 138 Fragments arranged by size: 1921 1712 1443 907 882 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3507 3539 7401 7433 3507 Size: 32 3862 32 3515 Fragments arranged by size: 3862 3515 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7441 XbaI T'CTAG_A Cuts at: 4294 4777 4294 Size: 483 6958 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3137 4413 3137 Size: 1276 6165 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7441 XmaI C'CCGG_G Cuts at: 1870 2102 4766 1870 Size: 232 2664 4545 Fragments arranged by size: 4545 2664 232 XmnI GAAnn'nnTTC Cuts at: 2427 4217 5570 2427 Size: 1790 1353 4298 Fragments arranged by size: 4298 1790 1353 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5249 276 Size: 53 83 186 936 3715 2468 Fragments arranged by size: 3715 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI RsrII SacII SanDI SapI SfiI SfoI SwaI