(Circular) (Six-base) MAPSORT of: pFN21AB9602.seq Check: 9416 from: 1 to: 6172 pFN21AB9602 6172 bp With 182 enzymes: SgfI * December 8, 2014 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3982 278 Size: 53 83 186 936 2446 2468 Fragments arranged by size: 2468 2446 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3515 1937 Size: 1578 4594 Acc65I G'GTAC_C Cuts at: 3493 3493 Size: 6172 AclI AA'CG_TT Cuts at: 1563 4299 4672 1563 Size: 2736 373 3063 Fragments arranged by size: 3063 2736 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4235 5619 5887 1632 Size: 2603 1384 268 1917 Fragments arranged by size: 2603 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5975 AflIII A'CryG_T Cuts at: 1203 2793 3469 5071 6048 1203 Size: 1590 676 1602 977 1327 Fragments arranged by size: 1602 1590 1327 977 676 AgeI A'CCGG_T Cuts at: 5003 5003 Size: 6172 AhdI GACnn_n'nnGTC Cuts at: 4901 4901 Size: 6172 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6172 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6172 AlwNI CAG_nnn'CTG Cuts at: 2308 2311 3915 5487 2308 Size: 3 1604 1572 2993 Fragments arranged by size: 2993 1604 1572 3 ApaI G_GGCC'C Cuts at: 2300 2300 Size: 6172 ApaLI G'TGCA_C Cuts at: 4229 5385 4229 Size: 1156 5016 ApoI r'AATT_y Cuts at: 1493 3481 3707 3859 5968 1493 Size: 1988 226 152 2109 1697 Fragments arranged by size: 2109 1988 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4726 160 Size: 872 3694 1606 Fragments arranged by size: 3694 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6172 AvaI C'yCGr_G Cuts at: 1870 1943 2078 2597 2749 3497 1870 Size: 73 135 519 152 748 4545 Fragments arranged by size: 4545 748 519 152 135 73 BamHI G'GATC_C Cuts at: 3502 3869 3502 Size: 367 5805 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2074 2245 2270 2416 2447 Size: 325 217 627 287 171 25 146 31 Cuts at: 2447 2453 2728 2807 2826 2990 3166 3184 3493 Size: 6 275 79 19 164 176 18 309 Cuts at: 3493 4948 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 325 309 287 275 217 176 171 164 146 79 31 25 19 18 6 BanII G_rGCy'C Cuts at: 1391 2300 2597 3491 1391 Size: 909 297 894 4072 Fragments arranged by size: 4072 909 894 297 BbeI G_GCGC'C Cuts at: 2274 2420 2451 2457 2830 2274 Size: 146 31 6 373 5616 Fragments arranged by size: 5616 373 146 31 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3441 928 Size: 971 1542 3659 Fragments arranged by size: 3659 1542 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4363 4397 1903 Size: 34 50 34 2342 34 3678 Fragments arranged by size: 3678 2342 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4064 5274 4064 Size: 1210 4962 BclI T'GATC_A Cuts at: 1548 1548 Size: 6172 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2425 2855 4783 Size: 107 122 71 631 605 753 430 1928 Cuts at: 4783 136 Size: 1525 Fragments arranged by size: 1928 1525 753 631 605 430 122 107 71 BglII A'GATC_T Cuts at: 6167 6167 Size: 6172 BlpI GC'TnA_GC Cuts at: 3589 3589 Size: 6172 Bme1580I G_kGCm'C Cuts at: 1790 2079 2300 3171 3189 4233 5389 1790 Size: 289 221 871 18 1044 1156 2573 Fragments arranged by size: 2573 1156 1044 871 289 221 18 BmgBI CAC'GTC Cuts at: 1318 2794 2997 3265 3300 3333 1318 Size: 1476 203 268 35 33 4157 Fragments arranged by size: 4157 1476 268 203 35 33 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3903 4861 442 Size: 416 53 83 399 24 2486 958 1753 Fragments arranged by size: 2486 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3158 1056 Size: 2102 4070 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4832 1239 Size: 3593 2579 Bpu10I CC'TnA_GC Cuts at: 3089 3089 Size: 6172 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4297 5162 5460 4297 Size: 865 298 5009 Fragments arranged by size: 5009 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4835 882 Size: 617 3336 2219 Fragments arranged by size: 3336 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3963 493 Size: 711 2759 2702 Fragments arranged by size: 2759 2702 711 BsaBI GATnn'nnATC Cuts at: 2582 6166 2582 Size: 3584 2588 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2271 2417 2448 2454 Size: 53 83 186 936 738 146 31 6 Cuts at: 2454 2827 3979 4361 4996 275 Size: 373 1152 382 635 1451 Fragments arranged by size: 1451 1152 936 738 635 382 373 186 146 83 53 31 6 BsaWI w'CCGG_w Cuts at: 4605 5003 5277 5424 5835 4605 Size: 398 274 147 411 4942 Fragments arranged by size: 4942 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3050 3056 3080 3086 3107 Size: 30 165 30 1647 6 24 6 21 Cuts at: 3107 3137 3203 3233 1178 Size: 30 66 30 4117 Fragments arranged by size: 4117 1647 165 66 30 30 30 30 24 21 6 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2480 3316 1186 Size: 1294 836 4042 Fragments arranged by size: 4042 1294 836 BseYI C'CCAG_C Cuts at: 2139 2301 2374 3008 3052 5375 5871 2139 Size: 162 73 634 44 2323 496 2440 Fragments arranged by size: 2440 2323 634 496 162 73 44 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2936 1163 Size: 573 1200 4399 Fragments arranged by size: 4399 1200 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2280 2343 3147 3622 4383 5411 Size: 275 186 279 63 804 475 761 1028 Cuts at: 5411 1540 Size: 2301 Fragments arranged by size: 2301 1028 804 761 475 279 275 186 63 BsiHKAI G_wGCw'C Cuts at: 4233 4318 5389 4233 Size: 85 1071 5016 Fragments arranged by size: 5016 1071 85 BsiWI C'GTAC_G Cuts at: 3123 3123 Size: 6172 BsmI GAATG_Cn' Cuts at: 3683 3776 3683 Size: 93 6079 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 2300 2597 3171 3189 3491 4233 Size: 399 289 221 297 574 18 302 742 Cuts at: 4233 4318 5389 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 574 399 302 297 289 221 85 18 BspEI T'CCGG_A Cuts at: 5835 5835 Size: 6172 BspHI T'CATG_A Cuts at: 2574 2637 4061 5791 2574 Size: 63 1424 1730 2955 Fragments arranged by size: 2955 1730 1424 63 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3237 3527 844 Size: 2393 290 3489 Fragments arranged by size: 3489 2393 290 BsrBI CCG'CTC Cuts at: 1394 1823 2832 4059 1394 Size: 429 1009 1227 3507 Fragments arranged by size: 3507 1227 1009 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4667 4841 65 Size: 1183 3419 174 1396 Fragments arranged by size: 3419 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2338 2450 2888 2912 3099 4816 5003 Size: 110 528 112 438 24 187 1717 187 Cuts at: 5003 1700 Size: 2869 Fragments arranged by size: 2869 1717 528 438 187 187 112 110 24 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6172 BssHII G'CGCG_C Cuts at: 1925 2873 1925 Size: 948 5224 BssSI C'ACGA_G Cuts at: 4232 5244 4232 Size: 1012 5160 BstAPI GCAn_nnn'nTGC Cuts at: 794 2311 794 Size: 1517 4655 BstBI TT'CG_AA Cuts at: 4976 4976 Size: 6172 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6172 BstYI r'GATC_y Cuts at: 1972 3117 3502 3869 4254 4271 5723 5809 5821 Size: 1145 385 367 385 17 1452 86 12 Cuts at: 5821 6167 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1145 385 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2113 2811 513 Size: 552 1048 698 3874 Fragments arranged by size: 3874 1048 698 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5433 Fragments arranged by size: 5433 683 56 BtsI GCAGTG_nn' Cuts at: 3688 4482 4502 3688 Size: 794 20 5358 Fragments arranged by size: 5358 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6172 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6137 DraI TTT'AAA Cuts at: 3476 3476 Size: 6172 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3104 3403 5179 809 Size: 507 1788 299 1776 1802 Fragments arranged by size: 1802 1788 1776 507 299 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2116 2277 2340 Size: 54 1425 50 275 18 286 161 63 Cuts at: 2340 2580 3144 3619 4508 6137 8 Size: 240 564 475 889 1629 43 Fragments arranged by size: 1629 1425 889 564 475 286 275 240 161 63 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2277 2340 3144 3619 1537 Size: 275 465 63 804 475 4090 Fragments arranged by size: 4090 804 475 465 275 63 EarI CTCTTCn'nnn_ Cuts at: 1353 4102 1353 Size: 2749 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2681 4759 5133 5279 387 Size: 1064 138 1092 2078 374 146 1280 Fragments arranged by size: 2078 1280 1092 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4235 4832 5619 5887 1239 Size: 393 2603 597 787 268 1524 Fragments arranged by size: 2603 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2296 2297 3198 3343 2296 Size: 1 901 145 5125 Fragments arranged by size: 5125 901 145 1 EcoRI G'AATT_C Cuts at: 3481 3859 3481 Size: 378 5794 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2875 3396 2875 Size: 521 5651 FspI TGC'GCA Cuts at: 4678 4678 Size: 6172 HaeII r_GCGC'y Cuts at: 1678 2274 2420 2451 2457 2830 3312 5319 1678 Size: 596 146 31 6 373 482 2007 2531 Fragments arranged by size: 2531 2007 596 482 373 146 31 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4813 4845 4887 4919 1373 Size: 32 135 32 3241 32 42 32 2626 Fragments arranged by size: 3241 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3039 3109 3226 3516 3762 669 Size: 988 281 1101 70 117 290 246 3079 Fragments arranged by size: 3079 1101 988 290 281 246 117 70 HindIII A'AGCT_T Cuts at: 748 748 Size: 6172 HpaI GTT'AAC Cuts at: 3762 3762 Size: 6172 KasI G'GCGC_C Cuts at: 2270 2416 2447 2453 2826 2270 Size: 146 31 6 373 5616 Fragments arranged by size: 5616 373 146 31 6 KpnI G_GTAC'C Cuts at: 3497 3497 Size: 6172 MfeI C'AATT_G Cuts at: 3771 3771 Size: 6172 MluI A'CGCG_T Cuts at: 6048 6048 Size: 6172 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3480 5286 5470 1305 Size: 2175 1806 184 2007 Fragments arranged by size: 2175 2007 1806 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2582 10 Size: 54 1425 343 750 3600 Fragments arranged by size: 3600 1425 750 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2576 2900 3302 4130 4489 4648 518 Size: 648 1410 324 402 828 359 159 2042 Fragments arranged by size: 2042 1410 828 648 402 359 324 159 MspA1I CmG'CkG Cuts at: 1606 2308 3588 4265 5413 5658 1606 Size: 702 1280 677 1148 245 2120 Fragments arranged by size: 2120 1280 1148 702 677 245 NaeI GCC'GGC Cuts at: 2452 2914 2452 Size: 462 5710 NarI GG'CG_CC Cuts at: 2271 2417 2448 2454 2827 2271 Size: 146 31 6 373 5616 Fragments arranged by size: 5616 373 146 31 6 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5620 NdeI CA'TA_TG Cuts at: 387 387 Size: 6172 NgoMIV G'CCGG_C Cuts at: 2450 2912 2450 Size: 462 5710 NheI G'CTAG_C Cuts at: 1052 3154 1052 Size: 2102 4070 NotI GC'GGCC_GC Cuts at: 3619 3619 Size: 6172 NspI r_CATG'y Cuts at: 1132 1229 3473 3530 5075 1132 Size: 97 2244 57 1545 2229 Fragments arranged by size: 2244 2229 1545 97 57 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6172 PciI A'CATG_T Cuts at: 3469 5071 3469 Size: 1602 4570 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6172 PmeI GTTT'AAAC Cuts at: 3476 3476 Size: 6172 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4241 4273 5754 5786 4241 Size: 32 1481 32 4627 Fragments arranged by size: 4627 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3198 3198 Size: 6172 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6172 PsiI TTA'TAA Cuts at: 3742 3742 Size: 6172 PspOMI G'GGCC_C Cuts at: 2296 2296 Size: 6172 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2522 2554 2636 2668 2522 Size: 32 82 32 6026 Fragments arranged by size: 6026 82 32 32 PstI C_TGCA'G Cuts at: 830 3524 830 Size: 2694 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6172 PvuII CAG'CTG Cuts at: 2308 2308 Size: 6172 SalI G'TCGA_C Cuts at: 1936 3514 1936 Size: 1578 4594 SbfI CC_TGCA'GG Cuts at: 3524 3524 Size: 6172 ScaI AGT'ACT Cuts at: 4420 6132 4420 Size: 1712 4460 SfcI C'TryA_G Cuts at: 826 1045 3025 3520 4655 5336 5527 826 Size: 219 1980 495 1135 681 191 1471 Fragments arranged by size: 1980 1471 1135 681 495 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2855 2855 Size: 6172 SfoI GGC'GCC Cuts at: 2272 2418 2449 2455 2828 2272 Size: 146 31 6 373 5616 Fragments arranged by size: 5616 373 146 31 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6172 SgrAI Cr'CCGG_yG Cuts at: 1700 2450 1700 Size: 750 5422 SmaI CCC'GGG Cuts at: 1872 2751 3499 1872 Size: 879 748 4545 Fragments arranged by size: 4545 879 748 SmlI C'TyrA_G Cuts at: 820 1017 1943 4276 5177 5439 820 Size: 197 926 2333 901 262 1553 Fragments arranged by size: 2333 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3963 493 Size: 3470 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6172 SphI G_CATG'C Cuts at: 1132 3530 1132 Size: 2398 3774 SspI AAT'ATT Cuts at: 5 52 4096 5 Size: 47 4044 2081 Fragments arranged by size: 4044 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6172 StyI C'CwwG_G Cuts at: 513 1065 3611 513 Size: 552 2546 3074 Fragments arranged by size: 3074 2546 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4199 4216 4369 4554 4999 325 Size: 1255 2619 17 153 185 445 1498 Fragments arranged by size: 2619 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4418 6130 96 Size: 274 80 33 51 1443 2441 1712 138 Fragments arranged by size: 2441 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2687 2719 3056 3088 6132 6164 2687 Size: 32 337 32 3044 32 2695 Fragments arranged by size: 3044 2695 337 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6172 XbaI T'CTAG_A Cuts at: 3508 3508 Size: 6172 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6172 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6172 XmaI C'CCGG_G Cuts at: 1870 2749 3497 1870 Size: 879 748 4545 Fragments arranged by size: 4545 879 748 XmnI GAAnn'nnTTC Cuts at: 2645 4301 2645 Size: 1656 4516 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3980 276 Size: 53 83 186 936 2446 2468 Fragments arranged by size: 2468 2446 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspXI RsrII SacI SacII SanDI SapI SexAI SrfI SwaI