################################## # NO CUT ENZYMES ( 168 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BanI BbeI BbrPI BbvCI BcgI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MslI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI SwaI TaqI TspRI Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AciI 96 CCGC CCGC(-3/-1) AcsI 145 AAATTT [GA]^AATT[CT] AlwNI 724 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 612 GGGCCC GGGCC^C ApoI 145 AAATTT [GA]^AATT[CT] AspHI 39 GTGCTC G[AT]GC[AT]^C AvaII 136 GGTCC G^G[AT]CC BamHI 510 GGATCC G^GATCC BciVI 649 GTATCC GTATCC(6/5) BpmI 42 CTCCAG CTGGAG(16/14) BsgI 200 GTGCAG GTGCAG(16/14) BsiEI 661 CGGTCG CG[GA][CT]^CG BsiHKAI 39 GTGCTC G[AT]GC[AT]^C BsmI 79 GAATGC GAATGC(1/-1) BsrFI 707 ACCGGC [GA]^CCGG[CT] BstAPI 74 GCACAGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cfr10I 707 ACCGGC [GA]^CCGG[CT] EcoRV 328 GATATC GAT^ATC EcoT14I 225 CCTTGG C^C[AT][AT]GG FauI 95 CCCGC CCCGC(4/6) HindIII 268 AAGCTT A^AGCTT MseI 596 TTAA T^TAA MspA1I 556 CAGCTG C[AC]G^C[GT]G PvuII 556 CAGCTG CAG^CTG SbfI 729 CCTGCAGG CCTGCA^GG SmlI 271 CTTGAG C^T[CT][GA]AG Sse8387I 729 CCTGCAGG CCTGCA^GG StuI 518 AGGCCT AGG^CCT StyI 225 CCTTGG C^C[AT][AT]GG TfiI 695 GAATC G^A[AT]TC Tru9I 596 TTAA T^TAA Tsp45I 220 GTCAC ^GT[GC]AC XcmI 359 CCAGCCTGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### BcnI 432 CCGGG CC^[GC]GG 469 CCGGG BstF5I 295 CATCC GGATG(2/0) 666 GGATG BstYI 510 GGATCC [GA]^GATC[CT] 725 AGATCC DdeI 474 CTCAG C^T[ACGT]AG 499 CTGAG DraII 611 GGGGCCC [GA]G^G[ACGT]CC[CT] 612 GGGCCCT EarI 317 CTCTTC CTCTTC(1/4) 617 CTCTTC EcoO109I 611 GGGGCCC [GA]G^G[ACGT]CC[CT] 612 GGGCCCT FokI 295 CATCC GGATG(9/13) 666 GGATG Ksp632I 317 CTCTTC CTCTTC(1/4) 617 CTCTTC MflI 510 GGATCC [GA]^GATC[CT] 725 AGATCC NciI 432 CCGGG CC^[GC]GG 469 CCGGG PleI 503 GACTC GAGTC(4/5) 604 GACTC PstI 522 CTGCAG CTGCA^G 730 CTGCAG SfaNI 667 GATGC GCATC(5/9) 644 GCATC SfcI 522 CTGCAG C^T[GA][CT]AG 730 CTGCAG XhoII 510 GGATCC [GA]^GATC[CT] 725 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AluI AlwI BanII BbsI BbvI BmyI BpuAI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstNI Cac8I Cfr13I DpnII EcoRII Fnu4HI HaeIII HapII HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MspI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TseI Tsp509I