(Circular) (Six-base) MAPSORT of: pFN21AE1745.seq Check: 2192 from: 1 to: 5446 pFN21AE1745 5446 bp With 182 enzymes: SgfI * August 16, 2010 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3256 278 Size: 53 83 186 936 1720 2468 Fragments arranged by size: 2468 1720 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2789 1937 Size: 852 4594 Acc65I G'GTAC_C Cuts at: 2767 2767 Size: 5446 AclI AA'CG_TT Cuts at: 1563 3573 3946 1563 Size: 2010 373 3063 Fragments arranged by size: 3063 2010 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2076 2521 2543 3509 4893 5161 1632 Size: 444 445 22 966 1384 268 1917 Fragments arranged by size: 1917 1384 966 445 444 268 22 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5249 AflIII A'CryG_T Cuts at: 1203 4345 5322 1203 Size: 3142 977 1327 Fragments arranged by size: 3142 1327 977 AgeI A'CCGG_T Cuts at: 4277 4277 Size: 5446 AhdI GACnn_n'nnGTC Cuts at: 4175 4175 Size: 5446 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5446 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5446 AlwNI CAG_nnn'CTG Cuts at: 2734 3189 4761 2734 Size: 455 1572 3419 Fragments arranged by size: 3419 1572 455 ApaI G_GGCC'C Cuts at: 2621 2621 Size: 5446 ApaLI G'TGCA_C Cuts at: 3503 4659 3503 Size: 1156 4290 ApoI r'AATT_y Cuts at: 1493 2150 2755 2981 3133 5242 1493 Size: 657 605 226 152 2109 1697 Fragments arranged by size: 2109 1697 657 605 226 152 AseI AT'TA_AT Cuts at: 160 1032 4000 160 Size: 872 2968 1606 Fragments arranged by size: 2968 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5446 AvaI C'yCGr_G Cuts at: 1870 1943 2771 1870 Size: 73 828 4545 Fragments arranged by size: 4545 828 73 BamHI G'GATC_C Cuts at: 2515 2776 3143 2515 Size: 261 367 4818 Fragments arranged by size: 4818 367 261 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2767 4222 618 Size: 325 217 627 980 1455 1842 Fragments arranged by size: 1842 1455 980 627 325 217 BanII G_rGCy'C Cuts at: 1391 2239 2480 2621 2765 1391 Size: 848 241 141 144 4072 Fragments arranged by size: 4072 848 241 144 141 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2265 2591 2683 928 Size: 971 366 326 92 3691 Fragments arranged by size: 3691 971 366 326 92 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3637 3671 1903 Size: 34 50 34 1616 34 3678 Fragments arranged by size: 3678 1616 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2665 3338 4548 2665 Size: 673 1210 3563 Fragments arranged by size: 3563 1210 673 BclI T'GATC_A Cuts at: 1548 1548 Size: 5446 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4057 136 Size: 107 122 71 631 605 2385 1525 Fragments arranged by size: 2385 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5441 5441 Size: 5446 BlpI GC'TnA_GC Cuts at: 2863 2863 Size: 5446 Bme1580I G_kGCm'C Cuts at: 1790 2081 2621 3507 4663 1790 Size: 291 540 886 1156 2573 Fragments arranged by size: 2573 1156 886 540 291 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5446 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3177 4135 442 Size: 416 53 83 399 24 1760 958 1753 Fragments arranged by size: 1760 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5446 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2032 4106 1239 Size: 793 2074 2579 Fragments arranged by size: 2579 2074 793 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2297 3571 4436 4734 2297 Size: 1274 865 298 3009 Fragments arranged by size: 3009 1274 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4109 882 Size: 617 2610 2219 Fragments arranged by size: 2610 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3237 493 Size: 711 2033 2702 Fragments arranged by size: 2702 2033 711 BsaBI GATnn'nnATC Cuts at: 5440 5440 Size: 5446 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3253 3635 4270 275 Size: 53 83 186 936 1720 382 635 1451 Fragments arranged by size: 1720 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3879 4277 4551 4698 5109 3879 Size: 398 274 147 411 4216 Fragments arranged by size: 4216 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5221 Fragments arranged by size: 5221 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5446 BseYI C'CCAG_C Cuts at: 2060 2448 2633 4649 5145 2060 Size: 388 185 2016 496 2361 Fragments arranged by size: 2361 2016 496 388 185 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2226 1163 Size: 573 490 4383 Fragments arranged by size: 4383 573 490 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2669 2896 3657 4685 1540 Size: 275 186 668 227 761 1028 2301 Fragments arranged by size: 2301 1028 761 668 275 227 186 BsiHKAI G_wGCw'C Cuts at: 2048 3507 3592 4663 2048 Size: 1459 85 1071 2831 Fragments arranged by size: 2831 1459 1071 85 BsmI GAATG_Cn' Cuts at: 2090 2957 3050 2090 Size: 867 93 4486 Fragments arranged by size: 4486 867 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2048 2081 2239 2480 2621 2765 3507 Size: 399 258 33 158 241 141 144 742 Cuts at: 3507 3592 4663 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 258 241 158 144 141 85 33 BspEI T'CCGG_A Cuts at: 5109 5109 Size: 5446 BspHI T'CATG_A Cuts at: 3335 5065 3335 Size: 1730 3716 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2801 844 Size: 1957 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3333 1394 Size: 429 1510 3507 Fragments arranged by size: 3507 1510 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3941 4115 65 Size: 1183 2693 174 1396 Fragments arranged by size: 2693 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2712 4090 4277 1700 Size: 110 902 1378 187 2869 Fragments arranged by size: 2869 1378 902 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5446 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5446 BssSI C'ACGA_G Cuts at: 3506 4518 3506 Size: 1012 4434 BstAPI GCAn_nnn'nTGC Cuts at: 794 2085 794 Size: 1291 4155 BstBI TT'CG_AA Cuts at: 4250 4250 Size: 5446 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5446 BstYI r'GATC_y Cuts at: 1972 2515 2730 2776 3143 3528 3545 4997 5083 Size: 543 215 46 367 385 17 1452 86 Cuts at: 5083 5095 5441 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 543 385 367 346 215 86 46 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4894 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4707 Fragments arranged by size: 4707 683 56 BtsI GCAGTG_nn' Cuts at: 2962 3756 3776 2962 Size: 794 20 4632 Fragments arranged by size: 4632 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5446 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5411 DraI TTT'AAA Cuts at: 2750 2750 Size: 5446 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4453 809 Size: 507 3137 1802 Fragments arranged by size: 3137 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2893 3782 5411 Size: 54 1425 50 275 18 1063 889 1629 Cuts at: 5411 8 Size: 43 Fragments arranged by size: 1629 1425 1063 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2893 1537 Size: 275 1081 4090 Fragments arranged by size: 4090 1081 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2328 2628 3376 1353 Size: 975 300 748 3423 Fragments arranged by size: 3423 975 748 300 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4033 4407 4553 387 Size: 1064 138 2444 374 146 1280 Fragments arranged by size: 2444 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2032 2076 2521 2543 3509 4106 4893 Size: 393 400 44 445 22 966 597 787 Cuts at: 4893 5161 1239 Size: 268 1524 Fragments arranged by size: 1524 966 787 597 445 400 393 268 44 22 EcoO109I rG'GnC_Cy Cuts at: 2617 2618 2617 Size: 1 5445 EcoRI G'AATT_C Cuts at: 2755 3133 2755 Size: 378 5068 EcoRV GAT'ATC Cuts at: 2335 2335 Size: 5446 FspI TGC'GCA Cuts at: 3952 3952 Size: 5446 HaeII r_GCGC'y Cuts at: 1678 4593 1678 Size: 2915 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2182 2214 4087 4119 4161 Size: 32 135 32 610 32 1873 32 42 Cuts at: 4161 4193 1373 Size: 32 2626 Fragments arranged by size: 2626 1873 610 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2790 3036 669 Size: 988 281 852 246 3079 Fragments arranged by size: 3079 988 852 281 246 HindIII A'AGCT_T Cuts at: 748 2273 748 Size: 1525 3921 HpaI GTT'AAC Cuts at: 3036 3036 Size: 5446 KpnI G_GTAC'C Cuts at: 2771 2771 Size: 5446 MfeI C'AATT_G Cuts at: 3045 3045 Size: 5446 MluI A'CGCG_T Cuts at: 5322 5322 Size: 5446 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2160 2649 4560 4744 1305 Size: 855 489 1911 184 2007 Fragments arranged by size: 2007 1911 855 489 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3624 Fragments arranged by size: 3624 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3404 3763 3922 518 Size: 648 2238 359 159 2042 Fragments arranged by size: 2238 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2563 2862 3539 4687 4932 1606 Size: 957 299 677 1148 245 2120 Fragments arranged by size: 2120 1148 957 677 299 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4894 NdeI CA'TA_TG Cuts at: 387 387 Size: 5446 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5446 NotI GC'GGCC_GC Cuts at: 2893 2893 Size: 5446 NspI r_CATG'y Cuts at: 1132 1229 2804 4349 1132 Size: 97 1575 1545 2229 Fragments arranged by size: 2229 1575 1545 97 PasI CC'CwG_GG Cuts at: 1348 2644 1348 Size: 1296 4150 PciI A'CATG_T Cuts at: 4345 4345 Size: 5446 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5446 PmeI GTTT'AAAC Cuts at: 2750 2750 Size: 5446 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3515 3547 5028 5060 3515 Size: 32 1481 32 3901 Fragments arranged by size: 3901 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5446 PsiI TTA'TAA Cuts at: 2171 2285 3016 2171 Size: 114 731 4601 Fragments arranged by size: 4601 731 114 PspOMI G'GGCC_C Cuts at: 2617 2617 Size: 5446 PstI C_TGCA'G Cuts at: 830 2531 2739 2798 830 Size: 1701 208 59 3478 Fragments arranged by size: 3478 1701 208 59 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5446 PvuII CAG'CTG Cuts at: 2563 2563 Size: 5446 SalI G'TCGA_C Cuts at: 1936 2788 1936 Size: 852 4594 SbfI CC_TGCA'GG Cuts at: 2739 2798 2739 Size: 59 5387 ScaI AGT'ACT Cuts at: 3694 5406 3694 Size: 1712 3734 SfcI C'TryA_G Cuts at: 826 1045 2527 2735 2794 3929 4610 4801 826 Size: 219 1482 208 59 1135 681 191 1471 Fragments arranged by size: 1482 1471 1135 681 219 208 191 59 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5446 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5446 SmaI CCC'GGG Cuts at: 1872 2773 1872 Size: 901 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2276 3550 4451 4713 820 Size: 197 926 333 1274 901 262 1553 Fragments arranged by size: 1553 1274 926 901 333 262 197 SnaBI TAC'GTA Cuts at: 493 3237 493 Size: 2744 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5446 SphI G_CATG'C Cuts at: 1132 2804 1132 Size: 1672 3774 SspI AAT'ATT Cuts at: 5 52 3370 5 Size: 47 3318 2081 Fragments arranged by size: 3318 2081 47 StuI AGG'CCT Cuts at: 1517 2525 1517 Size: 1008 4438 StyI C'CwwG_G Cuts at: 513 1065 2230 2885 513 Size: 552 1165 655 3074 Fragments arranged by size: 3074 1165 655 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3473 3490 3643 3828 4273 325 Size: 1255 1893 17 153 185 445 1498 Fragments arranged by size: 1893 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3692 5404 96 Size: 274 80 33 51 1443 1715 1712 138 Fragments arranged by size: 1715 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2379 2411 5406 5438 2379 Size: 32 2995 32 2387 Fragments arranged by size: 2995 2387 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5446 XbaI T'CTAG_A Cuts at: 2782 2782 Size: 5446 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2371 2371 Size: 5446 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5446 XmaI C'CCGG_G Cuts at: 1870 2771 1870 Size: 901 4545 XmnI GAAnn'nnTTC Cuts at: 3575 3575 Size: 5446 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3254 276 Size: 53 83 186 936 1720 2468 Fragments arranged by size: 2468 1720 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI