################################## # NO CUT ENZYMES ( 179 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BanII BbeI BbrPI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmFI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DpnII DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeII MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI MvnI NaeI NarI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I Tth111I TthHB8I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AspHI 162 GAGCAC G[AT]GC[AT]^C AvaI 377 CCCGGG C^[CT]CG[GA]G BanI 448 GGCACC G^G[CT][GA]CC BbsI 415 GTCTTC GAAGAC(2/6) BbvCI 153 GCTGAGG CCTCAGC(-5/-2) BglI 189 GCCTGCTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 415 GTCTTC GAAGAC(2/6) BseRI 145 GAGGAG GAGGAG(10/8) BsiHKAI 162 GAGCAC G[AT]GC[AT]^C BsmAI 287 GAGAC GTCTC(1/5) BsmI 64 GCATTC GAATGC(1/-1) BsoBI 377 CCCGGG C^[CT]CG[GA]G BsrDI 73 GCAATG GCAATG(2/0) BsrFI 296 ACCGGC [GA]^CCGG[CT] CfoI 474 GCGC GCG^C Cfr10I 296 ACCGGC [GA]^CCGG[CT] DraII 308 AGGACCT [GA]G^G[ACGT]CC[CT] DsaI 435 CCATGG C^C[GA][CT]GG EaeI 427 CGGCCA [CT]^GGCC[GA] EcoO109I 308 AGGACCT [GA]G^G[ACGT]CC[CT] EcoT14I 435 CCATGG C^C[AT][AT]GG FauI 336 CCCGC CCCGC(4/6) HgaI 344 GCGTC GACGC(5/10) HhaI 474 GCGC GCG^C HinP1I 474 GCGC G^CGC HinfI 214 GAGTC G^A[ACGT]TC HphI 22 GGTGA GGTGA(8/7) MspA1I 111 CAGCTG C[AC]G^C[GT]G NcoI 435 CCATGG C^CATGG PflMI 430 CCATGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 214 GAGTC GAGTC(4/5) PpuMI 308 AGGACCT [GA]G^G[AT]CC[CT] PvuII 111 CAGCTG CAG^CTG SmaI 377 CCCGGG CCC^GGG StyI 435 CCATGG C^C[AT][AT]GG Van91I 430 CCATGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 377 CCCGGG C^CCGGG XmaI 377 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AfaI 315 GTAC GT^AC 483 GTAC BcnI 377 CCCGG CC^[GC]GG 378 CCGGG BfaI 184 CTAG C^TAG 194 CTAG BpmI 267 CTGGAG CTGGAG(16/14) 469 CTGGAG BsrI 104 ACTGG ACTGG(1/-1) 227 CCAGT HapII 297 CCGG C^CGG 378 CCGG HpaII 297 CCGG C^CGG 378 CCGG MaeI 184 CTAG C^TAG 194 CTAG MboII 232 GAAGA GAAGA(8/7) 416 TCTTC MspI 297 CCGG C^CGG 378 CCGG NciI 377 CCCGG CC^[GC]GG 378 CCGGG RsaI 315 GTAC GT^AC 483 GTAC ######################################### # 3 OR MORE CUT ENZYMES ( 29 enzymes) # ######################################### AciI AluI AlwNI AvaII BbvI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstNI Cac8I Cfr13I DdeI EcoRII Fnu4HI HaeIII ItaI MnlI MvaI MwoI NlaIII NlaIV Sau96I ScrFI SfcI TseI TspRI