################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Aor51HI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstUI BstZ17I CelII Cfr10I ClaI CpoI DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgrAI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Alw44I 555 GTGCAC G^TGCAC ApaI 1343 GGGCCC GGGCC^C ApaLI 555 GTGCAC G^TGCAC Asp700I 1578 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 391 CCCGGG C^[CT]CG[GA]G BanI 1396 GGCACC G^G[CT][GA]CC BfaI 838 CTAG C^TAG BsaAI 610 TACGTG [CT]AC^GT[GA] BsmBI 274 CGTCTC CGTCTC(1/5) BsmI 1521 GAATGC GAATGC(1/-1) BsoBI 391 CCCGGG C^[CT]CG[GA]G BspHI 1220 TCATGA T^CATGA BstAPI 177 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1178 TTCGAA TT^CGAA BstYI 903 GGATCT [GA]^GATC[CT] DraI 1144 TTTAAA TTT^AAA DraIII 928 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 419 CCGTGG C^C[GA][CT]GG EcoRV 1190 GATATC GAT^ATC EcoT14I 791 CCTTGG C^C[AT][AT]GG HaeII 1349 AGCGCC [GA]GCGC^[CT] MaeI 838 CTAG C^TAG MflI 903 GGATCT [GA]^GATC[CT] NspV 1178 TTCGAA TT^CGAA PflMI 718 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 799 GAGTC GAGTC(4/5) PshAI 1243 GACCAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 481 CAGCTG CAG^CTG RcaI 1220 TCATGA T^CATGA SfuI 1178 TTCGAA TT^CGAA SmaI 391 CCCGGG CCC^GGG SspI 997 AATATT AAT^ATT StuI 1149 AGGCCT AGG^CCT StyI 791 CCTTGG C^C[AT][AT]GG Van91I 718 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 903 GGATCT [GA]^GATC[CT] XmaCI 391 CCCGGG C^CCGGG XmaI 391 CCCGGG C^CCGGG XmnI 1578 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AlwI 1211 GATCC GGATC(4/5) 903 GGATC BglI 400 GCCTTTAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1087 GCCTGTGTGGC BpmI 277 CTCCAG CTGGAG(16/14) 888 CTCCAG BsgI 454 GTGCAG GTGCAG(16/14) 626 GTGCAG BspMI 1561 ACCTGC ACCTGC(4/8) 456 GCAGGT BstEII 292 GGTGACC G^GT[ACGT]ACC 1044 GGTGACC BstPI 292 GGTGACC G^GT[ACGT]ACC 1044 GGTGACC BstXI 677 CCACACAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1309 CCAGGTGTGTGG DpnII 904 GATC ^GATC 1211 GATC DraII 501 GGGGCCT [GA]G^G[ACGT]CC[CT] 804 AGGCCCT EcoO109I 501 GGGGCCT [GA]G^G[ACGT]CC[CT] 804 AGGCCCT EcoO65I 292 GGTGACC G^GT[ACGT]ACC 1044 GGTGACC MboI 904 GATC ^GATC 1211 GATC MslI 135 CATCACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1372 CACCTGGATG NdeII 904 GATC ^GATC 1211 GATC SacI 168 GAGCTC GAGCT^C 1360 GAGCTC Sau3AI 904 GATC ^GATC 1211 GATC SmlI 379 CTCAAG C^T[CT][GA]AG 1030 CTCAAG TaqI 206 TCGA T^CGA 1179 TCGA TfiI 114 GAATC G^A[AT]TC 962 GATTC Tsp509I 1060 AATT ^AATT 1486 AATT TthHB8I 206 TCGA T^CGA 1179 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AciI AfaI AluI AlwNI AocI AspHI AvaII BanII BbvI BcnI BmyI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Bsu36I Cac8I CfoI Cfr13I DdeI EarI Eco81I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboII MnlI MseI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV NspI RsaI Sau96I ScrFI SfaNI Tru9I TseI Tsp45I TspRI