(Linear) (Six-base) MAPSORT of: KIEE4133.seq Check: 8701 from: 1 to: 1614 KIEE4133.orf With 182 enzymes: SgfI * March 22, 2012 17:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 447 1614 Size: 447 1167 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 248 1174 1469 1614 Size: 248 926 295 145 Fragments arranged by size: 926 295 248 145 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 469 1614 Size: 469 1145 AlwNI CAG_nnn'CTG Cuts at: 0 56 145 183 808 1614 Size: 56 89 38 625 806 Fragments arranged by size: 806 625 89 56 38 ApaI G_GGCC'C Cuts at: 0 1347 1614 Size: 1347 267 ApaLI G'TGCA_C Cuts at: 0 555 1614 Size: 555 1059 AvaI C'yCGr_G Cuts at: 0 391 1614 Size: 391 1223 BanI G'GyrC_C Cuts at: 0 1396 1614 Size: 1396 218 BanII G_rGCy'C Cuts at: 0 172 1347 1364 1614 Size: 172 1175 17 250 Fragments arranged by size: 1175 250 172 17 BglI GCCn_nnn'nGGC Cuts at: 0 406 1093 1614 Size: 406 687 521 Fragments arranged by size: 687 521 406 Bme1580I G_kGCm'C Cuts at: 0 348 559 1347 1614 Size: 348 211 788 267 Fragments arranged by size: 788 348 267 211 BmrI ACTGGGnnnn_n' Cuts at: 0 29 827 1614 Size: 29 798 787 Fragments arranged by size: 798 787 29 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 851 1614 Size: 851 763 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 262 873 1614 Size: 262 611 741 Fragments arranged by size: 741 611 262 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 364 1015 1614 Size: 364 651 599 Fragments arranged by size: 651 599 364 BsaAI yAC'GTr Cuts at: 0 612 1614 Size: 612 1002 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 85 115 1076 1106 1114 1144 1614 Size: 85 30 961 30 8 30 470 Fragments arranged by size: 961 470 85 30 30 30 8 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 110 379 442 484 1180 1614 Size: 110 269 63 42 696 434 Fragments arranged by size: 696 434 269 110 63 42 BseYI C'CCAG_C Cuts at: 0 124 483 522 1346 1454 1614 Size: 124 359 39 824 108 160 Fragments arranged by size: 824 359 160 124 108 39 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 475 647 1614 Size: 475 172 967 Fragments arranged by size: 967 475 172 BsiHKAI G_wGCw'C Cuts at: 0 172 559 817 1364 1614 Size: 172 387 258 547 250 Fragments arranged by size: 547 387 258 250 172 BsmI GAATG_Cn' Cuts at: 0 1527 1614 Size: 1527 87 BsmBI CGTCTCn'nnnn_ Cuts at: 0 280 1614 Size: 280 1334 Bsp1286I G_dGCh'C Cuts at: 0 172 348 559 817 1347 1364 1614 Size: 172 176 211 258 530 17 250 Fragments arranged by size: 530 258 250 211 176 172 17 BspHI T'CATG_A Cuts at: 0 1220 1614 Size: 1220 394 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 447 1570 1614 Size: 447 1123 44 Fragments arranged by size: 1123 447 44 BstAPI GCAn_nnn'nTGC Cuts at: 0 183 1614 Size: 183 1431 BstBI TT'CG_AA Cuts at: 0 1179 1614 Size: 1179 435 BstEII G'GTnAC_C Cuts at: 0 292 1044 1614 Size: 292 752 570 Fragments arranged by size: 752 570 292 BstXI CCAn_nnnn'nTGG Cuts at: 0 684 1316 1614 Size: 684 632 298 Fragments arranged by size: 684 632 298 BstYI r'GATC_y Cuts at: 0 903 1614 Size: 903 711 Bsu36I CC'TnA_GG Cuts at: 0 298 362 752 1238 1614 Size: 298 64 390 486 376 Fragments arranged by size: 486 390 376 298 64 BtgI C'CryG_G Cuts at: 0 419 1614 Size: 419 1195 BtsI GCAGTG_nn' Cuts at: 0 221 1614 Size: 221 1393 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1234 1269 1614 Size: 1234 35 345 Fragments arranged by size: 1234 345 35 DraI TTT'AAA Cuts at: 0 1146 1614 Size: 1146 468 DraIII CAC_nnn'GTG Cuts at: 0 933 1614 Size: 933 681 EarI CTCTTCn'nnn_ Cuts at: 0 28 419 791 1283 1490 1614 Size: 28 391 372 492 207 124 Fragments arranged by size: 492 391 372 207 124 28 EcoICRI GAG'CTC Cuts at: 0 170 1362 1614 Size: 170 1192 252 Fragments arranged by size: 1192 252 170 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 248 262 873 1174 1469 1614 Size: 248 14 611 301 295 145 Fragments arranged by size: 611 301 295 248 145 14 EcoO109I rG'GnC_Cy Cuts at: 0 502 805 1614 Size: 502 303 809 Fragments arranged by size: 809 502 303 EcoRV GAT'ATC Cuts at: 0 1192 1614 Size: 1192 422 HaeII r_GCGC'y Cuts at: 0 1353 1614 Size: 1353 261 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 629 661 1202 1234 1369 1401 1564 1596 Size: 629 32 541 32 135 32 163 32 Cuts at: 1596 1614 Size: 18 Fragments arranged by size: 629 541 163 135 32 32 32 32 18 MslI CAynn'nnrTG Cuts at: 0 139 1376 1614 Size: 139 1237 238 Fragments arranged by size: 1237 238 139 MspA1I CmG'CkG Cuts at: 0 201 449 483 696 772 1614 Size: 201 248 34 213 76 842 Fragments arranged by size: 842 248 213 201 76 34 NspI r_CATG'y Cuts at: 0 190 543 762 1614 Size: 190 353 219 852 Fragments arranged by size: 852 353 219 190 PasI CC'CwG_GG Cuts at: 0 105 550 808 1614 Size: 105 445 258 806 Fragments arranged by size: 806 445 258 105 PflMI CCAn_nnn'nTGG Cuts at: 0 724 1614 Size: 724 890 PfoI T'CCnGG_A Cuts at: 0 278 1614 Size: 278 1336 PshAI GACnn'nnGTC Cuts at: 0 1247 1614 Size: 1247 367 PspOMI G'GGCC_C Cuts at: 0 1343 1614 Size: 1343 271 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 623 655 1614 Size: 623 32 959 Fragments arranged by size: 959 623 32 PvuII CAG'CTG Cuts at: 0 483 1614 Size: 483 1131 SacI G_AGCT'C Cuts at: 0 172 1364 1614 Size: 172 1192 250 Fragments arranged by size: 1192 250 172 SmaI CCC'GGG Cuts at: 0 393 1614 Size: 393 1221 SmlI C'TyrA_G Cuts at: 0 379 1030 1614 Size: 379 651 584 Fragments arranged by size: 651 584 379 SspI AAT'ATT Cuts at: 0 999 1614 Size: 999 615 StuI AGG'CCT Cuts at: 0 1151 1614 Size: 1151 463 StyI C'CwwG_G Cuts at: 0 791 1614 Size: 791 823 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 280 1308 1614 Size: 280 1028 306 Fragments arranged by size: 1028 306 280 TatI w'GTAC_w Cuts at: 0 630 909 1614 Size: 630 279 705 Fragments arranged by size: 705 630 279 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 415 447 1076 1108 1614 Size: 415 32 629 32 506 Fragments arranged by size: 629 506 415 32 32 XmaI C'CCGG_G Cuts at: 0 391 1614 Size: 391 1223 XmnI GAAnn'nnTTC Cuts at: 0 1582 1614 Size: 1582 32 Enzymes that do cut: AarI AcuI AlfI AlwNI ApaI ApaLI AvaI BanI BanII BglI Bme1580I BmrI BplI BpmI BpuEI BsaAI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI DraI DraIII EarI EcoICRI Eco57MI EcoO109I EcoRV HaeII Hin4I MslI MspA1I NspI PasI PflMI PfoI PshAI PspOMI PsrI PvuII SacI SmaI SmlI SspI StuI StyI TaqII TatI TstI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI Bpu10I BsaI BsaBI BsaHI BsaWI BsiEI BsiWI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstZ17I BtgZI ClaI DrdI EaeI EagI EciI EcoNI EcoRI FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PpuMI PsiI PspXI PstI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SwaI Tth111I XbaI XcmI XhoI ZraI