################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflIII AgeI AhdI Alw44I AocI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BbeI BbsI BbvCI BcgI BciVI BclI BlnI BlpI Bpu1102I BpuAI BsaHI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HindIII HpaI KasI KpnI KspI MfeI MluI MluNI MroI MscI MunI MvnI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SpeI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccI 20 GTCTAC GT^[AC][GT]AC AcsI 346 AAATTC [GA]^AATT[CT] Aor51HI 232 AGCGCT AGC^GCT ApaI 238 GGGCCC GGGCC^C ApoI 346 AAATTC [GA]^AATT[CT] Asp700I 342 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 1240 GTGCTC G[AT]GC[AT]^C BamHI 1353 GGATCC G^GATCC BbrPI 178 CACGTG CAC^GTG BfaI 329 CTAG C^TAG BglI 1253 GCCGGCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1302 AGATCT A^GATCT BsaBI 1267 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1130 GGTCTC GGTCTC(1/5) BsaWI 121 TCCGGT [AT]^CCGG[AT] BsgI 769 GTGCAG GTGCAG(16/14) BsiHKAI 1240 GTGCTC G[AT]GC[AT]^C BsmI 517 GCATTC GAATGC(1/-1) BsrFI 1253 GCCGGC [GA]^CCGG[CT] BstAPI 1233 GCATGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cfr10I 1253 GCCGGC [GA]^CCGG[CT] EarI 135 GAAGAG CTCTTC(1/4) Eco47III 232 AGCGCT AGC^GCT FauI 1250 CCCGC CCCGC(4/6) HincII 1023 GTTGAC GT[CT]^[GA]AC HindII 1023 GTTGAC GT[CT]^[GA]AC Ksp632I 135 GAAGAG CTCTTC(1/4) MaeI 329 CTAG C^TAG MamI 1267 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MslI 11 CACTGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 1253 GCCGGC GCC^GGC NgoMIV 1253 GCCGGC G^CCGGC PmaCI 178 CACGTG CAC^GTG PmlI 178 CACGTG CAC^GTG PstI 130 CTGCAG CTGCA^G SexAI 975 ACCTGGT A^CC[AT]GGT SmaI 71 CCCGGG CCC^GGG SnaBI 413 TACGTA TAC^GTA SphI 1233 GCATGC GCATG^C StuI 139 AGGCCT AGG^CCT Tsp509I 347 AATT ^AATT XcmI 665 CCATGTTGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 71 CCCGGG C^CCGGG XmaI 71 CCCGGG C^CCGGG XmnI 342 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AflII 801 CTTAAG C^TTAAG 940 CTTAAG AvaI 71 CCCGGG C^[CT]CG[GA]G 1124 CTCGGG AvaII 936 GGTCC G^G[AT]CC 966 GGACC BanI 41 GGTGCC G^G[CT][GA]CC 208 GGTGCC BanII 238 GGGCCC G[GA]GC[CT]^C 533 GGGCTC BfrI 801 CTTAAG C^TTAAG 940 CTTAAG BsmAI 508 GTCTC GTCTC(1/5) 1131 GTCTC BsoBI 71 CCCGGG C^[CT]CG[GA]G 1124 CTCGGG BstXI 150 CCAGCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 926 CCAGGGATCTGG CfoI 76 GCGC GCG^C 233 GCGC EcoT14I 51 CCTTGG C^C[AT][AT]GG 764 CCAAGG HaeII 75 GGCGCT [GA]GCGC^[CT] 232 AGCGCT HhaI 76 GCGC GCG^C 233 GCGC HinP1I 76 GCGC G^CGC 233 GCGC HinfI 252 GAATC G^A[ACGT]TC 706 GAATC MspA1I 659 CAGCTG C[AC]G^C[GT]G 1074 CAGCTG NspI 1226 ACATGC [GA]CATG^[CT] 1233 GCATGC PvuII 659 CAGCTG CAG^CTG 1074 CAGCTG StyI 51 CCTTGG C^C[AT][AT]GG 764 CCAAGG TaqI 537 TCGA T^CGA 972 TCGA TfiI 252 GAATC G^A[AT]TC 706 GAATC TthHB8I 537 TCGA T^CGA 972 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI BbvI BcnI BmyI BpmI BsaAI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MseI MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI Tru9I TseI Tsp45I TspRI XhoII