(Circular) (Six-base) MAPSORT of: pFN21AB0433.seq Check: 5668 from: 1 to: 6073 pFN21AB0433 6073 bp With 182 enzymes: SgfI * August 16, 2010 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3883 278 Size: 53 83 186 936 2347 2468 Fragments arranged by size: 2468 2347 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2026 3416 1937 Size: 89 1390 4594 Fragments arranged by size: 4594 1390 89 Acc65I G'GTAC_C Cuts at: 3394 3394 Size: 6073 AclI AA'CG_TT Cuts at: 1563 4200 4573 1563 Size: 2637 373 3063 Fragments arranged by size: 3063 2637 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2039 3061 4136 5520 5788 1632 Size: 407 1022 1075 1384 268 1917 Fragments arranged by size: 1917 1384 1075 1022 407 268 AfeI AGC'GCT Cuts at: 2239 2239 Size: 6073 AflII C'TTAA_G Cuts at: 820 1017 2806 2945 820 Size: 197 1789 139 3948 Fragments arranged by size: 3948 1789 197 139 AflIII A'CryG_T Cuts at: 1203 4972 5949 1203 Size: 3769 977 1327 Fragments arranged by size: 3769 1327 977 AgeI A'CCGG_T Cuts at: 4904 4904 Size: 6073 AhdI GACnn_n'nnGTC Cuts at: 4802 4802 Size: 6073 AleI CACnn'nnGTG Cuts at: 1166 2020 1166 Size: 854 5219 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2315 1184 Size: 1131 4942 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3092 3124 3092 Size: 32 6041 AlwNI CAG_nnn'CTG Cuts at: 2203 2591 2714 3300 3816 5388 2203 Size: 388 123 586 516 1572 2888 Fragments arranged by size: 2888 1572 586 516 388 123 ApaI G_GGCC'C Cuts at: 2247 2247 Size: 6073 ApaLI G'TGCA_C Cuts at: 4130 5286 4130 Size: 1156 4917 ApoI r'AATT_y Cuts at: 1493 2351 3382 3608 3760 5869 1493 Size: 858 1031 226 152 2109 1697 Fragments arranged by size: 2109 1697 1031 858 226 152 AseI AT'TA_AT Cuts at: 160 1032 4627 160 Size: 872 3595 1606 Fragments arranged by size: 3595 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6073 AvaI C'yCGr_G Cuts at: 1870 1943 2076 3129 3398 1870 Size: 73 133 1053 269 4545 Fragments arranged by size: 4545 1053 269 133 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3044 3077 3044 Size: 33 6040 BamHI G'GATC_C Cuts at: 3358 3403 3770 3358 Size: 45 367 5661 Fragments arranged by size: 5661 367 45 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2046 2213 3394 4849 618 Size: 325 217 627 259 167 1181 1455 1842 Fragments arranged by size: 1842 1455 1181 627 325 259 217 167 BanII G_rGCy'C Cuts at: 1391 2247 2542 3392 1391 Size: 856 295 850 4072 Fragments arranged by size: 4072 856 850 295 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5102 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4264 4298 1903 Size: 34 2327 34 3678 Fragments arranged by size: 3678 2327 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3965 5175 3965 Size: 1210 4863 BclI T'GATC_A Cuts at: 1548 1548 Size: 6073 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3264 4684 Size: 107 122 71 631 605 337 1255 1420 Cuts at: 4684 136 Size: 1525 Fragments arranged by size: 1525 1420 1255 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 3307 6068 3307 Size: 2761 3312 BlpI GC'TnA_GC Cuts at: 3490 3490 Size: 6073 Bme1580I G_kGCm'C Cuts at: 1790 2051 2218 2247 2913 3231 3289 4134 5290 Size: 261 167 29 666 318 58 845 1156 Cuts at: 5290 1790 Size: 2573 Fragments arranged by size: 2573 1156 845 666 318 261 167 58 29 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6073 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2212 3019 3804 Size: 416 53 83 399 24 795 807 785 Cuts at: 3804 4762 442 Size: 958 1753 Fragments arranged by size: 1753 958 807 795 785 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6073 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2308 2500 3123 4733 1239 Size: 1069 192 623 1610 2579 Fragments arranged by size: 2579 1610 1069 623 192 Bpu10I CC'TnA_GC Cuts at: 2584 3354 2584 Size: 770 5303 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2363 4198 5063 5361 2363 Size: 1835 865 298 3075 Fragments arranged by size: 3075 1835 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3141 4736 882 Size: 617 1642 1595 2219 Fragments arranged by size: 2219 1642 1595 617 BsaAI yAC'GTr Cuts at: 493 1204 2185 2420 2639 3864 493 Size: 711 981 235 219 1225 2702 Fragments arranged by size: 2702 1225 981 711 235 219 BsaBI GATnn'nnATC Cuts at: 3276 6067 3276 Size: 2791 3282 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3880 4262 4897 275 Size: 53 83 186 936 2347 382 635 1451 Fragments arranged by size: 2347 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2126 4506 4904 5178 5325 5736 2126 Size: 2380 398 274 147 411 2463 Fragments arranged by size: 2463 2380 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5848 Fragments arranged by size: 5848 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2330 2399 3158 1186 Size: 1144 69 759 4101 Fragments arranged by size: 4101 1144 759 69 BseYI C'CCAG_C Cuts at: 2240 2819 2906 5276 5772 2240 Size: 579 87 2370 496 2541 Fragments arranged by size: 2541 2370 579 496 87 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2795 1163 Size: 573 1059 4441 Fragments arranged by size: 4441 1059 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3523 4284 5312 1540 Size: 275 186 1522 761 1028 2301 Fragments arranged by size: 2301 1522 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3249 4134 4219 5290 3249 Size: 885 85 1071 4032 Fragments arranged by size: 4032 1071 885 85 BsmI GAATG_Cn' Cuts at: 2522 3584 3677 2522 Size: 1062 93 4918 Fragments arranged by size: 4918 1062 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2051 2218 2247 2542 2913 3231 3249 Size: 399 261 167 29 295 371 318 18 Cuts at: 3249 3289 3392 4134 4219 5290 1391 Size: 40 103 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 371 318 295 261 167 103 85 40 29 18 BspEI T'CCGG_A Cuts at: 5736 5736 Size: 6073 BspHI T'CATG_A Cuts at: 3962 5692 3962 Size: 1730 4343 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3428 844 Size: 2584 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3960 1394 Size: 429 2137 3507 Fragments arranged by size: 3507 2137 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4568 4742 65 Size: 1183 3320 174 1396 Fragments arranged by size: 3320 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3258 4717 4904 1700 Size: 110 1448 1459 187 2869 Fragments arranged by size: 2869 1459 1448 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6073 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6073 BssSI C'ACGA_G Cuts at: 4133 5145 4133 Size: 1012 5061 BstAPI GCAn_nnn'nTGC Cuts at: 794 3244 794 Size: 2450 3623 BstBI TT'CG_AA Cuts at: 4877 4877 Size: 6073 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6073 BstXI CCAn_nnnn'nTGG Cuts at: 2162 2938 2162 Size: 776 5297 BstYI r'GATC_y Cuts at: 1972 2532 2935 3307 3358 3403 3770 4155 4172 Size: 560 403 372 51 45 367 385 17 Cuts at: 4172 5624 5710 5722 6068 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 560 403 385 372 367 346 86 51 45 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4582 Fragments arranged by size: 4582 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2135 3137 487 Size: 683 56 909 1002 3423 Fragments arranged by size: 3423 1002 909 683 56 BtsI GCAGTG_nn' Cuts at: 2409 3589 4383 4403 2409 Size: 1180 794 20 4079 Fragments arranged by size: 4079 1180 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6073 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2550 2585 529 Size: 35 1986 35 4017 Fragments arranged by size: 4017 1986 35 35 DraI TTT'AAA Cuts at: 3377 3377 Size: 6073 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5080 809 Size: 507 3764 1802 Fragments arranged by size: 3764 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3520 4409 6038 Size: 54 1425 50 275 18 1690 889 1629 Cuts at: 6038 8 Size: 43 Fragments arranged by size: 1690 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3520 1537 Size: 275 1708 4090 Fragments arranged by size: 4090 1708 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2135 4003 1353 Size: 782 1868 3423 Fragments arranged by size: 3423 1868 782 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4660 5034 5180 387 Size: 1064 138 3071 374 146 1280 Fragments arranged by size: 3071 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2039 2308 2500 3061 3123 4136 4733 Size: 393 407 269 192 561 62 1013 597 Cuts at: 4733 5520 5788 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1013 787 597 561 407 393 269 268 192 62 EcoRI G'AATT_C Cuts at: 3382 3760 3382 Size: 378 5695 FspI TGC'GCA Cuts at: 4579 4579 Size: 6073 HaeII r_GCGC'y Cuts at: 1678 2084 2241 5220 1678 Size: 406 157 2979 2531 Fragments arranged by size: 2979 2531 406 157 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2108 2134 2140 2166 2266 Size: 32 135 32 536 26 6 26 100 Cuts at: 2266 2298 2524 2556 2567 2599 3112 3144 4714 Size: 32 226 32 11 32 513 32 1570 Cuts at: 4714 4746 4788 4820 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1570 536 513 226 135 100 42 32 32 32 32 32 32 32 32 26 26 11 6 HincII GTy'rAC Cuts at: 669 1657 1938 3030 3417 3663 669 Size: 988 281 1092 387 246 3079 Fragments arranged by size: 3079 1092 988 387 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6073 HpaI GTT'AAC Cuts at: 3663 3663 Size: 6073 KpnI G_GTAC'C Cuts at: 3398 3398 Size: 6073 MfeI C'AATT_G Cuts at: 3672 3672 Size: 6073 MluI A'CGCG_T Cuts at: 5949 5949 Size: 6073 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5187 5371 1305 Size: 3882 184 2007 Fragments arranged by size: 3882 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4251 Fragments arranged by size: 4251 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2020 4031 4390 4549 518 Size: 648 854 2011 359 159 2042 Fragments arranged by size: 2042 2011 854 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2666 3081 3489 4166 5314 5559 1606 Size: 1060 415 408 677 1148 245 2120 Fragments arranged by size: 2120 1148 1060 677 415 408 245 NaeI GCC'GGC Cuts at: 3260 3260 Size: 6073 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4582 Fragments arranged by size: 4582 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6073 NgoMIV G'CCGG_C Cuts at: 3258 3258 Size: 6073 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6073 NotI GC'GGCC_GC Cuts at: 3520 3520 Size: 6073 NspI r_CATG'y Cuts at: 1132 1229 3235 3242 3431 4976 1132 Size: 97 2006 7 189 1545 2229 Fragments arranged by size: 2229 2006 1545 189 97 7 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6073 PciI A'CATG_T Cuts at: 4972 4972 Size: 6073 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6073 PfoI T'CCnGG_A Cuts at: 2355 2355 Size: 6073 PmeI GTTT'AAAC Cuts at: 3377 3377 Size: 6073 PmlI CAC'GTG Cuts at: 2185 2185 Size: 6073 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4142 4174 5655 5687 4142 Size: 32 1481 32 4528 Fragments arranged by size: 4528 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6073 PsiI TTA'TAA Cuts at: 3643 3643 Size: 6073 PspOMI G'GGCC_C Cuts at: 2243 2243 Size: 6073 PstI C_TGCA'G Cuts at: 830 2139 3425 830 Size: 1309 1286 3478 Fragments arranged by size: 3478 1309 1286 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6073 PvuII CAG'CTG Cuts at: 2666 3081 2666 Size: 415 5658 SalI G'TCGA_C Cuts at: 1936 3415 1936 Size: 1479 4594 SbfI CC_TGCA'GG Cuts at: 3425 3425 Size: 6073 ScaI AGT'ACT Cuts at: 4321 6033 4321 Size: 1712 4361 SexAI A'CCwGG_T Cuts at: 2980 2980 Size: 6073 SfcI C'TryA_G Cuts at: 826 1045 2135 2304 3043 3421 4556 5237 5428 Size: 219 1090 169 739 378 1135 681 191 Cuts at: 5428 826 Size: 1471 Fragments arranged by size: 1471 1135 1090 739 681 378 219 191 169 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6073 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6073 SmaI CCC'GGG Cuts at: 1872 2078 3400 1872 Size: 206 1322 4545 Fragments arranged by size: 4545 1322 206 SmlI C'TyrA_G Cuts at: 820 1017 1943 2342 2806 2945 4177 5078 5340 Size: 197 926 399 464 139 1232 901 262 Cuts at: 5340 820 Size: 1553 Fragments arranged by size: 1553 1232 926 901 464 399 262 197 139 SnaBI TAC'GTA Cuts at: 493 2420 3864 493 Size: 1927 1444 2702 Fragments arranged by size: 2702 1927 1444 SpeI A'CTAG_T Cuts at: 152 152 Size: 6073 SphI G_CATG'C Cuts at: 1132 3242 3431 1132 Size: 2110 189 3774 Fragments arranged by size: 3774 2110 189 SrfI GCCC'GGGC Cuts at: 2078 2078 Size: 6073 SspI AAT'ATT Cuts at: 5 52 3997 5 Size: 47 3945 2081 Fragments arranged by size: 3945 2081 47 StuI AGG'CCT Cuts at: 1517 2146 1517 Size: 629 5444 StyI C'CwwG_G Cuts at: 513 1065 2004 2056 2769 3512 513 Size: 552 939 52 713 743 3074 Fragments arranged by size: 3074 939 743 713 552 52 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2782 4100 4117 4270 4455 4900 325 Size: 1255 1202 1318 17 153 185 445 1498 Fragments arranged by size: 1498 1318 1255 1202 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4319 6031 96 Size: 274 80 33 51 1443 2342 1712 138 Fragments arranged by size: 2342 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6033 6065 6033 Size: 32 6041 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6073 XbaI T'CTAG_A Cuts at: 3409 3409 Size: 6073 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2677 2677 Size: 6073 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6073 XmaI C'CCGG_G Cuts at: 1870 2076 3398 1870 Size: 206 1322 4545 Fragments arranged by size: 4545 1322 206 XmnI GAAnn'nnTTC Cuts at: 2351 4202 2351 Size: 1851 4222 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3881 276 Size: 53 83 186 936 2347 2468 Fragments arranged by size: 2468 2347 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SwaI