##################################
# NO CUT ENZYMES (  199 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AgeI        AhdI        AluI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AvrII       BalI        BamHI       BbeI        BbrPI       
BbsI        BbvCI       BbvI        BcgI        BclI        
BcnI        BfrI        BglI        BglII       BlnI        
BlpI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmBI       BsmFI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstF5I      BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      Cac8I       CelII       Cfr10I      
Cfr13I      ClaI        CpoI        DdeI        DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoRI       
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
Fnu4HI      FokI        FseI        HaeII       HaeIII      
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        ItaI        KasI        KpnI        Ksp632I     
KspI        MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MspA1I      MunI        
MvnI        MwoI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
Sau96I      SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StuI        StyI        SwaI        TaqI        TseI        
Tth111I     TthHB8I     Van91I      XbaI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AcsI           26  AAATTT               [GA]^AATT[CT]
AflIII        392  ACATGT               A^C[GA][CT]GT
ApoI           26  AAATTT               [GA]^AATT[CT]
AviII         375  TGCGCA               TGC^GCA
BanI          312  GGTGCC               G^G[CT][GA]CC
BciVI         447  GTATCC               GTATCC(6/5)
BfaI          269  CTAG                 C^TAG
BsaI          434  GAGACC               GGTCTC(1/5)
BsaJI          98  CCTGGG               C^C[ACGT][ACGT]GG
BsaWI         450  TCCGGT               [AT]^CCGG[AT]
BsmAI         434  GAGAC                GTCTC(1/5)
BspLU11I      392  ACATGT               A^CATGT
BsrI          460  CCAGT                ACTGG(1/-1)
BstEII        247  GGTAACC              G^GT[ACGT]ACC
BstPI         247  GGTAACC              G^GT[ACGT]ACC
CfoI          376  GCGC                 GCG^C
DpnII         424  GATC                 ^GATC
EcoO65I       247  GGTAACC              G^GT[ACGT]ACC
FspI          375  TGCGCA               TGC^GCA
HapII         451  CCGG                 C^CGG
HhaI          376  GCGC                 GCG^C
HinP1I        376  GCGC                 G^CGC
HinfI         476  GAATC                G^A[ACGT]TC
HpaII         451  CCGG                 C^CGG
MaeI          269  CTAG                 C^TAG
MaeII         230  ACGT                 A^CGT
MboI          424  GATC                 ^GATC
MboII          39  TCTTC                GAAGA(8/7)
MspI          451  CCGG                 C^CGG
NdeII         424  GATC                 ^GATC
PstI          366  CTGCAG               CTGCA^G
Sau3AI        424  GATC                 ^GATC
SfaNI         188  GATGC                GCATC(5/9)
SfcI          366  CTGCAG               C^T[GA][CT]AG
SspI          355  AATATT               AAT^ATT
TfiI          476  GAATC                G^A[AT]TC
XcmI           88  CCACAGTTTACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

BanII          66  GGGCTC               G[GA]GC[CT]^C
              101  GGGCTC              
BmyI           66  GGGCTC               G[AGT]GC[ACT]^C
              101  GGGCTC              
Bsp1286I       66  GGGCTC               G[AGT]GC[ACT]^C
              101  GGGCTC              
BspMI         162  GCAGGT               ACCTGC(4/8)
              368  GCAGGT              
BsrDI         144  GCAATG               GCAATG(2/0)
              157  GCAATG              
HphI          294  TCACC                GGTGA(8/7)
              465  TCACC               
MslI          126  CATCAATATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              295  CACCACAGTG          
NspI          392  ACATGT               [GA]CATG^[CT]
              409  GCATGT              
Tsp45I        293  GTCAC                ^GT[GC]AC
              413  GTGAC               
TspRI         300  CAGTG                CA[GC]TG[ACGT][ACGT]^
              325  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   12 enzymes) #
#########################################

BssKI       BstNI       EcoRII      MaeIII      MnlI        
MseI        MvaI        NlaIII      NlaIV       ScrFI       
Tru9I       Tsp509I