(Linear) (Six-base) MAPSORT of: KIEE5196.seq  Check: 7489  from: 1  to: 483



KIEE5196.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:50  ..

AflIII A'CryG_T

Cuts at:      0     392     483 
   Size:        392      91
 
AleI CACnn'nnGTG

Cuts at:      0     299     483 
   Size:        299     184
 
ApoI r'AATT_y

Cuts at:      0      26     483 
   Size:         26     457
 
BanI G'GyrC_C

Cuts at:      0     312     483 
   Size:        312     171
 
BanII G_rGCy'C

Cuts at:      0      70     105     483 
   Size:         70      35     378

  Fragments arranged by size: 

                 378      70      35
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     458     483 
   Size:        458      25
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     428     483 
   Size:        428      55
 
BsaWI w'CCGG_w

Cuts at:      0     450     483 
   Size:        450      33
 
BseYI C'CCAG_C

Cuts at:      0     119     140     483 
   Size:        119      21     343

  Fragments arranged by size: 

                 343     119      21
 
Bsp1286I G_dGCh'C

Cuts at:      0      70     105     483 
   Size:         70      35     378

  Fragments arranged by size: 

                 378      70      35
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     153     359     483 
   Size:        153     206     124

  Fragments arranged by size: 

                 206     153     124
 
BsrDI GCAATG_nn'

Cuts at:      0     151     164     483 
   Size:        151      13     319

  Fragments arranged by size: 

                 319     151      13
 
BstEII G'GTnAC_C

Cuts at:      0     247     483 
   Size:        247     236
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     201     483 
   Size:        201     282
 
FspI TGC'GCA

Cuts at:      0     377     483 
   Size:        377     106
 
MslI CAynn'nnrTG

Cuts at:      0     130     299     483 
   Size:        130     169     184

  Fragments arranged by size: 

                 184     169     130
 
NspI r_CATG'y

Cuts at:      0     396     413     483 
   Size:        396      17      70

  Fragments arranged by size: 

                 396      70      17
 
PciI A'CATG_T

Cuts at:      0     392     483 
   Size:        392      91
 
PstI C_TGCA'G

Cuts at:      0     370     483 
   Size:        370     113
 
SfcI C'TryA_G

Cuts at:      0     366     483 
   Size:        366     117
 
SspI AAT'ATT

Cuts at:      0     357     483 
   Size:        357     126
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     296     328     483 
   Size:        296      32     155

  Fragments arranged by size: 

                 296     155      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      95     483 
   Size:         95     388
 

 Enzymes that do cut:

   AflIII     AleI     ApoI     BanI    BanII    BciVI     BsaI    BsaWI
    BseYI Bsp1286I    BspMI    BsrDI   BstEII    BtgZI     FspI     MslI
     NspI     PciI     PstI     SfcI     SspI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI
    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaHI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     StuI     StyI     SwaI    TaqII     TatI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI