Restriction map for
pFN21AE5164
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AluI AlwI AlwNI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspI AvaI AviII AvrII BamHI
BanII BbeI BbsI BbvCI BcgI
BciVI BclI BcnI BfrI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstBI
BstUI BstXI BstYI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI EarI EclXI
Eco52I EcoNI EcoRI EcoT14I EcoT22I
FauI FbaI FseI FspI HapII
HgaI Hin1I HincII HindII HindIII
HpaI HpaII HphI KasI KpnI
Ksp632I KspI MamI MfeI MflI
MluI MroI MseI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmeI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
Tru9I Tsp509I Tth111I TthHB8I Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AciI 111 GCGG CCGC(-3/-1)
AfaI 96 GTAC GT^AC
AhdI 386 GACGTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 127 CCTGAGG CC^T[ACGT]AGG
Aor51HI 203 AGCGCT AGC^GCT
AspEI 386 GACGTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 81 TGGCCA TGG^CCA
BbrPI 118 CACGTG CAC^GTG
BfaI 383 CTAG C^TAG
BglI 83 GCCAGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 118 CACGTG [CT]AC^GT[GA]
BsaJI 344 CCAGGG C^C[ACGT][ACGT]GG
BseRI 130 GAGGAG GAGGAG(10/8)
BsmAI 133 GAGAC GTCTC(1/5)
BsmFI 329 GGGAC GGGAC(10/14)
BstAPI 114 GCAACACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 409 GGTAACC G^GT[ACGT]ACC
BstPI 409 GGTAACC G^GT[ACGT]ACC
Bsu36I 127 CCTGAGG CC^T[ACGT]AGG
CfoI 204 GCGC GCG^C
DdeI 128 CTGAG C^T[ACGT]AG
DpnII 148 GATC ^GATC
DraII 13 AGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 81 TGGCCA [CT]^GGCC[GA]
Eam1105I 386 GACGTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 203 AGCGCT AGC^GCT
Eco81I 127 CCTGAGG CC^T[ACGT]AGG
EcoO109I 13 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 409 GGTAACC G^GT[ACGT]ACC
HaeII 203 AGCGCT [GA]GCGC^[CT]
HhaI 204 GCGC GCG^C
HinP1I 204 GCGC G^CGC
HinfI 104 GATTC G^A[ACGT]TC
MaeI 383 CTAG C^TAG
MboI 148 GATC ^GATC
MluNI 81 TGGCCA TGG^CCA
MscI 81 TGGCCA TGG^CCA
MslI 298 CATGAGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 148 GATC ^GATC
NspI 92 GCATGT [GA]CATG^[CT]
PmaCI 118 CACGTG CAC^GTG
PmlI 118 CACGTG CAC^GTG
PpuMI 13 AGGACCC [GA]G^G[AT]CC[CT]
RsaI 96 GTAC GT^AC
Sau3AI 148 GATC ^GATC
SfaNI 168 GATGC GCATC(5/9)
TfiI 104 GATTC G^A[AT]TC
Tsp45I 316 GTCAC ^GT[GC]AC
XcmI 69 CCAGCCCTCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AflIII 117 ACACGT A^C[GA][CT]GT
387 ACGTGT
Alw44I 121 GTGCAC G^TGCAC
427 GTGCAC
ApaLI 121 GTGCAC G^TGCAC
427 GTGCAC
AvaII 14 GGACC G^G[AT]CC
66 GGTCC
BanI 348 GGCACC G^G[CT][GA]CC
420 GGTGCC
BbvI 254 GCAGC GCAGC(8/12)
39 GCTGC
BsiYI 47 CCCTGCTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
73 CCCTCCTATGG
BslI 47 CCCTGCTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
73 CCCTCCTATGG
BsrDI 23 CATTGC GCAATG(2/0)
396 CATTGC
BssKI 84 CCAGG ^CC[ACGT]GG
344 CCAGG
BstF5I 219 CATCC GGATG(2/0)
87 GGATG
BstNI 84 CCAGG CC^[AT]GG
344 CCAGG
Cac8I 200 GCAAGC GC[ACGT]^[ACGT]GC
376 GCGAGC
EcoRII 84 CCAGG ^CC[AT]GG
344 CCAGG
EcoRV 229 GATATC GAT^ATC
362 GATATC
FokI 219 CATCC GGATG(9/13)
87 GGATG
HaeIII 82 GGCC GG^CC
369 GGCC
MaeII 119 ACGT A^CGT
387 ACGT
MaeIII 316 GTCAC ^GT[ACGT]AC
410 GTAAC
MboII 180 GAAGA GAAGA(8/7)
226 GAAGA
MvaI 84 CCAGG CC^[AT]GG
344 CCAGG
ScrFI 84 CCAGG CC^[ACGT]GG
344 CCAGG
TseI 39 GCTGC G^C[AT]GC
254 GCAGC
TspRI 144 CAGTG CA[GC]TG[ACGT][ACGT]^
196 CACTG
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# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AspHI BmyI BsiHKAI Bsp1286I Cfr13I
Fnu4HI ItaI MnlI MwoI NlaIII
NlaIV PstI Sau96I SfcI