(Linear) (Six-base) MAPSORT of: KIEE5164.seq  Check: 3392  from: 1  to: 441



KIEE5164.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:49  ..

AfeI AGC'GCT

Cuts at:      0     205     441 
   Size:        205     236
 
AflIII A'CryG_T

Cuts at:      0     117     387     441 
   Size:        117     270      54

  Fragments arranged by size: 

                 270     117      54
 
AhdI GACnn_n'nnGTC

Cuts at:      0     391     441 
   Size:        391      50
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     372     441 
   Size:        372      69
 
ApaLI G'TGCA_C

Cuts at:      0     121     427     441 
   Size:        121     306      14

  Fragments arranged by size: 

                 306     121      14
 
BanI G'GyrC_C

Cuts at:      0     348     420     441 
   Size:        348      72      21

  Fragments arranged by size: 

                 348      72      21
 
BglI GCCn_nnn'nGGC

Cuts at:      0      89     441 
   Size:         89     352
 
Bme1580I G_kGCm'C

Cuts at:      0     125     351     431     441 
   Size:        125     226      80      10

  Fragments arranged by size: 

                 226     125      80      10
 
BmgBI CAC'GTC

Cuts at:      0     388     441 
   Size:        388      53
 
BsaAI yAC'GTr

Cuts at:      0     120     441 
   Size:        120     321
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     145     441 
   Size:        145     296
 
BseYI C'CCAG_C

Cuts at:      0      51      57     441 
   Size:         51       6     384

  Fragments arranged by size: 

                 384      51       6
 
BsiHKAI G_wGCw'C

Cuts at:      0     125     382     431     441 
   Size:        125     257      49      10

  Fragments arranged by size: 

                 257     125      49      10
 
Bsp1286I G_dGCh'C

Cuts at:      0     125     351     382     431     441 
   Size:        125     226      31      49      10

  Fragments arranged by size: 

                 226     125      49      31      10
 
BsrDI GCAATG_nn'

Cuts at:      0      22     395     441 
   Size:         22     373      46

  Fragments arranged by size: 

                 373      46      22
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     120     441 
   Size:        120     321
 
BstEII G'GTnAC_C

Cuts at:      0     409     441 
   Size:        409      32
 
Bsu36I CC'TnA_GG

Cuts at:      0     128     441 
   Size:        128     313
 
BtsI GCAGTG_nn'

Cuts at:      0     150     195     441 
   Size:        150      45     246

  Fragments arranged by size: 

                 246     150      45
 
EaeI y'GGCC_r

Cuts at:      0      81     441 
   Size:         81     360
 
EcoO109I rG'GnC_Cy

Cuts at:      0      14     441 
   Size:         14     427
 
EcoRV GAT'ATC

Cuts at:      0     231     364     441 
   Size:        231     133      77

  Fragments arranged by size: 

                 231     133      77
 
HaeII r_GCGC'y

Cuts at:      0     207     441 
   Size:        207     234
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     177     209     377     409     441 
   Size:        177      32     168      32      32

  Fragments arranged by size: 

                 177     168      32      32      32
 
MscI TGG'CCA

Cuts at:      0      83     441 
   Size:         83     358
 
MslI CAynn'nnrTG

Cuts at:      0     302     441 
   Size:        302     139
 
NspI r_CATG'y

Cuts at:      0      96     441 
   Size:         96     345
 
PmlI CAC'GTG

Cuts at:      0     120     441 
   Size:        120     321
 
PpuMI rG'GwC_Cy

Cuts at:      0      14     441 
   Size:         14     427
 
PstI C_TGCA'G

Cuts at:      0      11     256     361     441 
   Size:         11     245     105      80

  Fragments arranged by size: 

                 245     105      80      11
 
SfcI C'TryA_G

Cuts at:      0       7     252     357     441 
   Size:          7     245     105      84

  Fragments arranged by size: 

                 245     105      84       7
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     366     441 
   Size:        366      75
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      76     441 
   Size:         76     365
 

 Enzymes that do cut:

     AfeI   AflIII     AhdI     AlfI    ApaLI     BanI     BglI Bme1580I
    BmgBI    BsaAI    BseRI    BseYI  BsiHKAI Bsp1286I    BsrDI   BstAPI
   BstEII   Bsu36I     BtsI     EaeI EcoO109I    EcoRV    HaeII    Hin4I
     MscI     MslI     NspI     PmlI    PpuMI     PstI     SfcI    TaqII
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII     AgeI
     AleI     AloI    AlwNI     ApaI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI    BglII     BlpI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI
    BsaXI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI    BstBI    BstXI
    BstYI  BstZ17I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI
     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI