################################## # NO CUT ENZYMES ( 97 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AvrII BamHI BanI BbeI BbvCI BciVI BfrI BglII BlnI BlpI Bpu1102I BsaHI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrFI BsrGI BssHII Bst1107I BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DrdI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FseI Hin1I HindIII HpaI KasI KpnI MfeI MluI MroI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PinAI PmeI PpuMI PshAI PshBI Psp1406I RsrII SalI SapI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SspBI SspI SwaI XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AhdI 2124 GACTCTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1621 GTGCAC G^TGCAC ApaLI 1621 GTGCAC G^TGCAC Asp700I 1758 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 2124 GACTCTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 247 CTCGAG C^[CT]CG[GA]G AviII 1871 TGCGCA TGC^GCA BcgI 1683 GCACAGTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 647 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaI 1200 GAGACC GGTCTC(1/5) BsaWI 629 ACCGGA [AT]^CCGG[AT] BsiEI 1049 CGATCG CG[GA][CT]^CG BsmBI 1183 GAGACG CGTCTC(1/5) BsoBI 247 CTCGAG C^[CT]CG[GA]G BstBI 1500 TTCGAA TT^CGAA BstF5I 933 GGATG GGATG(2/0) BstYI 350 AGATCC [GA]^GATC[CT] DraIII 2266 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 2124 GACTCTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 1762 GAATTC G^AATTC EcoT14I 1613 CCATGG C^C[AT][AT]GG FokI 933 GGATG GGATG(9/13) FspI 1871 TGCGCA TGC^GCA HgaI 318 GCGTC GACGC(5/10) HincII 1159 GTCAAC GT[CT]^[GA]AC HindII 1159 GTCAAC GT[CT]^[GA]AC MaeIII 1547 GTGAC ^GT[ACGT]AC MflI 350 AGATCC [GA]^GATC[CT] NcoI 1613 CCATGG C^CATGG NdeI 1531 CATATG CA^TATG NspV 1500 TTCGAA TT^CGAA PaeR7I 247 CTCGAG C^TCGAG PvuI 1049 CGATCG CGAT^CG SacI 253 GAGCTC GAGCT^C SbfI 669 CCTGCAGG CCTGCA^GG SfuI 1500 TTCGAA TT^CGAA SphI 656 GCATGC GCATG^C Sse8387I 669 CCTGCAGG CCTGCA^GG StuI 323 AGGCCT AGG^CCT StyI 1613 CCATGG C^C[AT][AT]GG Tsp45I 1547 GTGAC ^GT[GC]AC XcmI 506 CCATCTGGGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 247 CTCGAG C^TCGAG XhoII 350 AGATCC [GA]^GATC[CT] XmnI 1758 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AlwI 351 GATCC GGATC(4/5) 1311 GATCC AspI 334 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC 1078 GACATGGTC BalI 1597 TGGCCA TGG^CCA 1844 TGGCCA BanII 253 GAGCTC G[GA]GC[CT]^C 916 GGGCTC BbsI 331 GAAGAC GAAGAC(2/6) 367 GAAGAC BclI 437 TGATCA T^GATCA 443 TGATCA BcnI 100 CCGGG CC^[GC]GG 981 CCGGG BfaI 376 CTAG C^TAG 2143 CTAG BpuAI 331 GAAGAC GAAGAC(2/6) 367 GAAGAC BspHI 440 TCATGA T^CATGA 1307 TCATGA BspMI 668 ACCTGC ACCTGC(4/8) 672 GCAGGT BssSI 199 CACGAG CACGAG(-5/-1) 40 CTCGTG BstAPI 999 GCAGGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1527 GCAGCATATGC BstXI 119 CCAAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1463 CCAGCAAGCTGG DraII 11 AGGCCCC [GA]G^G[ACGT]CC[CT] 1554 AGGCCCT EcoNI 230 CCTCCATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1164 CCTGATGAAGG EcoO109I 11 AGGCCCC [GA]G^G[ACGT]CC[CT] 1554 AGGCCCT FbaI 437 TGATCA T^GATCA 443 TGATCA HaeII 36 GGCGCT [GA]GCGC^[CT] 1002 GGCGCT KspI 16 CCGCGG CCGC^GG 71 CCGCGG MaeI 376 CTAG C^TAG 2143 CTAG MluNI 1597 TGGCCA TGG^CCA 1844 TGGCCA MscI 1597 TGGCCA TGG^CCA 1844 TGGCCA NciI 100 CCGGG CC^[GC]GG 981 CCGGG PflFI 334 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC 1078 GACATGGTC PvuII 970 CAGCTG CAG^CTG 2164 CAGCTG RcaI 440 TCATGA T^CATGA 1307 TCATGA SacII 16 CCGCGG CCGC^GG 71 CCGCGG SmlI 247 CTCGAG C^T[CT][GA]AG 1435 CTCAAG Tth111I 334 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC 1078 GACATGGTC ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwNI ApoI AspHI AvaII BbrPI BbvI BmyI BpmI BsaAI BsaBI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrBI BsrI BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraI DsaI EaeI EarI EcoRII FauI Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MamI MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I Van91I