(Circular) (Six-base) MAPSORT of: pFN21AE2085.seq Check: 1648 from: 1 to: 6982 pFN21AE2085 6982 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2668 2668 Size: 6982 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4792 278 Size: 53 83 186 936 3256 2468 Fragments arranged by size: 3256 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4325 1937 Size: 2388 4594 Acc65I G'GTAC_C Cuts at: 4303 4303 Size: 6982 AclI AA'CG_TT Cuts at: 1563 5109 5482 1563 Size: 3546 373 3063 Fragments arranged by size: 3546 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3638 3689 3811 5045 6429 6697 1632 Size: 2006 51 122 1234 1384 268 1917 Fragments arranged by size: 2006 1917 1384 1234 268 122 51 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6785 AflIII A'CryG_T Cuts at: 1203 2187 2412 3913 5881 6858 1203 Size: 984 225 1501 1968 977 1327 Fragments arranged by size: 1968 1501 1327 984 977 225 AgeI A'CCGG_T Cuts at: 5813 5813 Size: 6982 AhdI GACnn_n'nnGTC Cuts at: 4134 5711 4134 Size: 1577 5405 AleI CACnn'nnGTG Cuts at: 1166 2483 1166 Size: 1317 5665 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2501 3902 1184 Size: 1317 1401 4264 Fragments arranged by size: 4264 1401 1317 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3470 3502 3470 Size: 32 6950 AlwNI CAG_nnn'CTG Cuts at: 3010 3793 3895 4725 6297 3010 Size: 783 102 830 1572 3695 Fragments arranged by size: 3695 1572 830 783 102 ApaLI G'TGCA_C Cuts at: 3626 5039 6195 3626 Size: 1413 1156 4413 Fragments arranged by size: 4413 1413 1156 ApoI r'AATT_y Cuts at: 1493 2705 3767 4017 4175 4291 4517 4669 6778 Size: 1212 1062 250 158 116 226 152 2109 Cuts at: 6778 1493 Size: 1697 Fragments arranged by size: 2109 1697 1212 1062 250 226 158 152 116 AseI AT'TA_AT Cuts at: 160 1032 5536 160 Size: 872 4504 1606 Fragments arranged by size: 4504 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6982 AvaI C'yCGr_G Cuts at: 1870 1943 2252 4307 1870 Size: 73 309 2055 4545 Fragments arranged by size: 4545 2055 309 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2954 2987 2954 Size: 33 6949 BamHI G'GATC_C Cuts at: 4312 4679 4312 Size: 367 6615 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4303 5758 618 Size: 325 217 627 2516 1455 1842 Fragments arranged by size: 2516 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2262 2925 4301 1391 Size: 871 663 1376 4072 Fragments arranged by size: 4072 1376 871 663 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2343 2379 928 Size: 971 444 36 5531 Fragments arranged by size: 5531 971 444 36 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3677 3711 5173 5207 1903 Size: 34 1740 34 1462 34 3678 Fragments arranged by size: 3678 1740 1462 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4874 6084 4874 Size: 1210 5772 BclI T'GATC_A Cuts at: 1548 2442 2448 1548 Size: 894 6 6082 Fragments arranged by size: 6082 894 6 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2658 5593 136 Size: 107 122 71 631 605 986 2935 1525 Fragments arranged by size: 2935 1525 986 631 605 122 107 71 BglII A'GATC_T Cuts at: 6977 6977 Size: 6982 BlpI GC'TnA_GC Cuts at: 4399 4399 Size: 6982 Bme1580I G_kGCm'C Cuts at: 1790 3630 5043 6199 1790 Size: 1840 1413 1156 2573 Fragments arranged by size: 2573 1840 1413 1156 BmgBI CAC'GTC Cuts at: 1318 2344 1318 Size: 1026 5956 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4713 5671 442 Size: 416 53 83 399 24 3296 958 1753 Fragments arranged by size: 3296 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6982 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4146 4146 Size: 6982 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3043 3311 4212 5642 1239 Size: 1804 268 901 1430 2579 Fragments arranged by size: 2579 1804 1430 901 268 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3425 5107 5972 6270 3425 Size: 1682 865 298 4137 Fragments arranged by size: 4137 1682 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3199 5645 882 Size: 617 1700 2446 2219 Fragments arranged by size: 2446 2219 1700 617 BsaAI yAC'GTr Cuts at: 493 1204 2190 2415 3127 3914 4276 4773 493 Size: 711 986 225 712 787 362 497 2702 Fragments arranged by size: 2702 986 787 712 711 497 362 225 BsaBI GATnn'nnATC Cuts at: 2447 3059 3314 6976 2447 Size: 612 255 3662 2453 Fragments arranged by size: 3662 2453 612 255 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4789 5171 5806 275 Size: 53 83 186 936 3256 382 635 1451 Fragments arranged by size: 3256 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2634 5415 5813 6087 6234 6645 2634 Size: 2781 398 274 147 411 2971 Fragments arranged by size: 2971 2781 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2282 2312 2808 2838 3284 Size: 30 165 30 879 30 496 30 446 Cuts at: 3284 3314 3783 3813 1178 Size: 30 469 30 4347 Fragments arranged by size: 4347 879 496 469 446 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2270 2629 3299 3791 3851 1186 Size: 1084 359 670 492 60 4317 Fragments arranged by size: 4317 1084 670 492 359 60 BseYI C'CCAG_C Cuts at: 6185 6681 6185 Size: 496 6486 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2186 2243 3477 1163 Size: 573 450 57 1234 4668 Fragments arranged by size: 4668 1234 573 450 57 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3057 4432 5193 6221 1540 Size: 275 186 1056 1375 761 1028 2301 Fragments arranged by size: 2301 1375 1056 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2262 3630 3801 5043 5128 6199 2262 Size: 1368 171 1242 85 1071 3045 Fragments arranged by size: 3045 1368 1242 1071 171 85 BsmI GAATG_Cn' Cuts at: 4493 4586 4493 Size: 93 6889 BsmBI CGTCTCn'nnnn_ Cuts at: 3182 3182 Size: 6982 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2262 2925 3630 3801 4301 5043 5128 Size: 399 472 663 705 171 500 742 85 Cuts at: 5128 6199 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 705 663 500 472 399 171 85 BspEI T'CCGG_A Cuts at: 6645 6645 Size: 6982 BspHI T'CATG_A Cuts at: 2445 3312 4871 6601 2445 Size: 867 1559 1730 2826 Fragments arranged by size: 2826 1730 1559 867 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2668 2682 4337 844 Size: 1824 14 1655 3489 Fragments arranged by size: 3489 1824 1655 14 BsrBI CCG'CTC Cuts at: 1394 1823 2619 3157 3295 4869 1394 Size: 429 796 538 138 1574 3507 Fragments arranged by size: 3507 1574 796 538 429 138 BsrDI GCAATG_nn' Cuts at: 65 1248 5477 5651 65 Size: 1183 4229 174 1396 Fragments arranged by size: 4229 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5626 5813 1700 Size: 110 3816 187 2869 Fragments arranged by size: 3816 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6982 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6982 BssSI C'ACGA_G Cuts at: 2045 2204 5042 6054 2045 Size: 159 2838 1012 2973 Fragments arranged by size: 2973 2838 1012 159 BstAPI GCAn_nnn'nTGC Cuts at: 794 3010 3538 794 Size: 2216 528 4238 Fragments arranged by size: 4238 2216 528 BstBI TT'CG_AA Cuts at: 3506 5786 3506 Size: 2280 4702 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6982 BstXI CCAn_nnnn'nTGG Cuts at: 2131 3475 2131 Size: 1344 5638 BstYI r'GATC_y Cuts at: 1972 2355 4312 4679 5064 5081 6533 6619 6631 Size: 383 1957 367 385 17 1452 86 12 Cuts at: 6631 6977 1972 Size: 346 1977 Fragments arranged by size: 1977 1957 1452 385 383 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2021 2076 2628 3334 3618 3852 Size: 552 939 17 55 552 706 284 234 Cuts at: 3852 513 Size: 3643 Fragments arranged by size: 3643 939 706 552 552 284 234 55 17 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6243 Fragments arranged by size: 6243 683 56 BtsI GCAGTG_nn' Cuts at: 2428 4498 5292 5312 2428 Size: 2070 794 20 4098 Fragments arranged by size: 4098 2070 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6982 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3222 3257 529 Size: 35 2658 35 4254 Fragments arranged by size: 4254 2658 35 35 DraI TTT'AAA Cuts at: 2400 3738 3751 4286 2400 Size: 1338 13 535 5096 Fragments arranged by size: 5096 1338 535 13 DraIII CAC_nnn'GTG Cuts at: 4276 4276 Size: 6982 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5989 809 Size: 507 4673 1802 Fragments arranged by size: 4673 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2085 3602 3849 Size: 54 1425 50 275 18 255 1517 247 Cuts at: 3849 4278 4429 5318 6947 8 Size: 429 151 889 1629 43 Fragments arranged by size: 1629 1517 1425 889 429 275 255 247 151 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4429 1537 Size: 275 2617 4090 Fragments arranged by size: 4090 2617 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2709 2805 3788 4912 1353 Size: 1356 96 983 1124 3423 Fragments arranged by size: 3423 1356 1124 983 96 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 2209 5569 5943 6089 387 Size: 1064 138 435 185 3360 374 146 1280 Fragments arranged by size: 3360 1280 1064 435 374 185 146 138 EcoICRI GAG'CTC Cuts at: 2260 2260 Size: 6982 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3043 3311 3638 3689 3811 4212 5045 Size: 393 1411 268 327 51 122 401 833 Cuts at: 5045 5642 6429 6697 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1411 833 787 597 401 393 327 268 268 122 51 EcoNI CCTnn'n_nnAGG Cuts at: 2239 3173 2239 Size: 934 6048 EcoO109I rG'GnC_Cy Cuts at: 2017 3560 2017 Size: 1543 5439 EcoRI G'AATT_C Cuts at: 3767 4291 4669 3767 Size: 524 378 6080 Fragments arranged by size: 6080 524 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2050 3199 2050 Size: 1149 5833 FspI TGC'GCA Cuts at: 3878 5488 3878 Size: 1610 5372 HaeII r_GCGC'y Cuts at: 1678 2045 3011 6129 1678 Size: 367 966 3118 2531 Fragments arranged by size: 3118 2531 966 367 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2427 2459 3913 3945 4120 Size: 32 135 32 855 32 1454 32 175 Cuts at: 4120 4152 5623 5655 5697 5729 1373 Size: 32 1471 32 42 32 2626 Fragments arranged by size: 2626 1471 1454 855 175 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3166 4326 4572 669 Size: 988 281 1228 1160 246 3079 Fragments arranged by size: 3079 1228 1160 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6982 HpaI GTT'AAC Cuts at: 4572 4572 Size: 6982 KpnI G_GTAC'C Cuts at: 4307 4307 Size: 6982 MfeI C'AATT_G Cuts at: 4581 4581 Size: 6982 MluI A'CGCG_T Cuts at: 6858 6858 Size: 6982 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3081 3282 6096 6280 1305 Size: 1776 201 2814 184 2007 Fragments arranged by size: 2814 2007 1776 201 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3604 3851 10 Size: 54 1425 343 1772 247 3141 Fragments arranged by size: 3141 1772 1425 343 247 54 MslI CAynn'nnrTG Cuts at: 518 1166 2483 3617 3623 4940 5299 5458 518 Size: 648 1317 1134 6 1317 359 159 2042 Fragments arranged by size: 2042 1317 1317 1134 648 359 159 6 MspA1I CmG'CkG Cuts at: 1606 2023 2078 2977 4171 4398 5075 6223 6468 Size: 417 55 899 1194 227 677 1148 245 Cuts at: 6468 1606 Size: 2120 Fragments arranged by size: 2120 1194 1148 899 677 417 245 227 55 NcoI C'CATG_G Cuts at: 513 1065 2004 3618 513 Size: 552 939 1614 3877 Fragments arranged by size: 3877 1614 939 552 NdeI CA'TA_TG Cuts at: 387 3537 387 Size: 3150 3832 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6982 NotI GC'GGCC_GC Cuts at: 4429 4429 Size: 6982 NspI r_CATG'y Cuts at: 1132 1229 2665 3242 3487 3731 4340 5885 1132 Size: 97 1436 577 245 244 609 1545 2229 Fragments arranged by size: 2229 1545 1436 609 577 245 244 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6982 PciI A'CATG_T Cuts at: 5881 5881 Size: 6982 PflMI CCAn_nnn'nTGG Cuts at: 1108 2272 2935 3793 4276 1108 Size: 1164 663 858 483 3814 Fragments arranged by size: 3814 1164 858 663 483 PmeI GTTT'AAAC Cuts at: 4286 4286 Size: 6982 PmlI CAC'GTG Cuts at: 2190 2415 3127 3914 2190 Size: 225 712 787 5258 Fragments arranged by size: 5258 787 712 225 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5051 5083 6564 6596 5051 Size: 32 1481 32 5437 Fragments arranged by size: 5437 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6982 PsiI TTA'TAA Cuts at: 3363 4552 3363 Size: 1189 5793 PspXI vC'TCGA_Gb Cuts at: 2252 2252 Size: 6982 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2963 2995 2963 Size: 32 6950 PstI C_TGCA'G Cuts at: 830 2435 2493 2679 2916 3018 3223 3891 4334 Size: 1605 58 186 237 102 205 668 443 Cuts at: 4334 830 Size: 3478 Fragments arranged by size: 3478 1605 668 443 237 205 186 102 58 PvuI CG_AT'CG Cuts at: 2001 3057 2001 Size: 1056 5926 PvuII CAG'CTG Cuts at: 2977 4171 2977 Size: 1194 5788 SacI G_AGCT'C Cuts at: 2262 2262 Size: 6982 SacII CC_GC'GG Cuts at: 2024 2079 2024 Size: 55 6927 SalI G'TCGA_C Cuts at: 1936 4324 1936 Size: 2388 4594 SbfI CC_TGCA'GG Cuts at: 2679 4334 2679 Size: 1655 5327 ScaI AGT'ACT Cuts at: 5230 6942 5230 Size: 1712 5270 SfcI C'TryA_G Cuts at: 826 1045 2431 2489 2675 2912 3014 3219 3564 Size: 219 1386 58 186 237 102 205 345 Cuts at: 3564 3887 4330 5465 6146 6337 826 Size: 323 443 1135 681 191 1471 Fragments arranged by size: 1471 1386 1135 681 443 345 323 237 219 205 191 186 102 58 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6982 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6982 SmaI CCC'GGG Cuts at: 1872 4309 1872 Size: 2437 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2252 3440 5086 5987 6249 820 Size: 197 926 309 1188 1646 901 262 1553 Fragments arranged by size: 1646 1553 1188 926 901 309 262 197 SnaBI TAC'GTA Cuts at: 493 4773 493 Size: 4280 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6982 SphI G_CATG'C Cuts at: 1132 2665 4340 1132 Size: 1533 1675 3774 Fragments arranged by size: 3774 1675 1533 SspI AAT'ATT Cuts at: 5 52 4906 5 Size: 47 4854 2081 Fragments arranged by size: 4854 2081 47 StuI AGG'CCT Cuts at: 1517 2330 1517 Size: 813 6169 StyI C'CwwG_G Cuts at: 513 1065 2004 3618 4421 513 Size: 552 939 1614 803 3074 Fragments arranged by size: 3074 1614 939 803 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5009 5026 5179 5364 5809 325 Size: 1255 3429 17 153 185 445 1498 Fragments arranged by size: 3429 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3030 5228 6940 Size: 274 80 33 51 1443 1053 2198 1712 Cuts at: 6940 96 Size: 138 Fragments arranged by size: 2198 1712 1443 1053 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2282 2314 3783 3815 3930 3962 6942 6974 2282 Size: 32 1469 32 115 32 2980 32 2290 Fragments arranged by size: 2980 2290 1469 115 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2342 3086 1316 Size: 1026 744 5212 Fragments arranged by size: 5212 1026 744 XbaI T'CTAG_A Cuts at: 4318 4318 Size: 6982 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2518 2518 Size: 6982 XhoI C'TCGA_G Cuts at: 1943 2252 1943 Size: 309 6673 XmaI C'CCGG_G Cuts at: 1870 4307 1870 Size: 2437 4545 XmnI GAAnn'nnTTC Cuts at: 3767 5111 3767 Size: 1344 5638 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4790 276 Size: 53 83 186 936 3256 2468 Fragments arranged by size: 3256 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI