################################## # NO CUT ENZYMES ( 150 enzymes) # ################################## AatII AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspI AvaI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I CelII Cfr10I ClaI CpoI DraI DraII DraIII Eam1105I EarI Eco47III EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HapII HindIII HpaI HpaII Ksp632I KspI MamI MfeI MflI MluI MluNI MroI MscI MspI MunI NaeI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tsp45I Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Acc65I 60 GGTACC G^GTACC AccII 597 CGCG CG^CG AcyI 601 GGCGCC G[GA]^CG[CT]C AocI 838 CCTGAGG CC^T[ACGT]AGG Asp718I 60 GGTACC G^GTACC BanII 844 GAGCTC G[GA]GC[CT]^C BbeI 601 GGCGCC GGCGC^C BsaHI 601 GGCGCC G[GA]^CG[CT]C BseRI 841 GAGGAG GAGGAG(10/8) BsiEI 190 CGGCCG CG[GA][CT]^CG BsrDI 663 CATTGC GCAATG(2/0) BstUI 597 CGCG CG^CG Bsu36I 838 CCTGAGG CC^T[ACGT]AGG DrdI 370 GACCTGAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 190 CGGCCG [CT]^GGCC[GA] EagI 190 CGGCCG C^GGCCG EclXI 190 CGGCCG C^GGCCG Eco52I 190 CGGCCG C^GGCCG Eco81I 838 CCTGAGG CC^T[ACGT]AGG EcoNI 752 CCTGGTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HgaI 377 GCGTC GACGC(5/10) Hin1I 601 GGCGCC G[GA]^CG[CT]C HincII 457 GTCGAC GT[CT]^[GA]AC HindII 457 GTCGAC GT[CT]^[GA]AC KasI 601 GGCGCC G^GCGCC KpnI 60 GGTACC GGTAC^C MaeII 155 ACGT A^CGT MaeIII 710 GTTAC ^GT[ACGT]AC MboII 726 TCTTC GAAGA(8/7) MvnI 597 CGCG CG^CG NarI 601 GGCGCC GG^CGCC NcoI 143 CCATGG C^CATGG NspI 391 ACATGC [GA]CATG^[CT] PvuII 15 CAGCTG CAG^CTG SacI 844 GAGCTC GAGCT^C SalI 457 GTCGAC G^TCGAC SfcI 876 CTACAG C^T[GA][CT]AG SfoI 601 GGCGCC GGC^GCC TspRI 401 CACTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccI 457 GTCGAC GT^[AC][GT]AC 746 GTCTAC AcsI 169 AAATTT [GA]^AATT[CT] 962 AAATTC AlwI 7 GATCC GGATC(4/5) 659 GGATC ApoI 169 AAATTT [GA]^AATT[CT] 962 AAATTC BbvI 801 GCAGC GCAGC(8/12) 314 GCTGC BfaI 175 CTAG C^TAG 734 CTAG BpmI 283 CTCCAG CTGGAG(16/14) 320 CTGGAG BsrBI 280 CCGCTC CCGCTC(-3/-3) 868 CCGCTC BstXI 116 CCATCCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 335 CCAATATCTTGG CfoI 602 GCGC GCG^C 653 GCGC DsaI 143 CCATGG C^C[GA][CT]GG 193 CCGTGG EcoT14I 128 CCTTGG C^C[AT][AT]GG 143 CCATGG HaeII 601 GGCGCC [GA]GCGC^[CT] 652 AGCGCC HhaI 602 GCGC GCG^C 653 GCGC HinP1I 602 GCGC G^CGC 653 GCGC HphI 132 GGTGA GGTGA(8/7) 860 TCACC MaeI 175 CTAG C^TAG 734 CTAG MseI 545 TTAA T^TAA 581 TTAA MslI 531 CATTGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 621 CATAGCTGTG MspA1I 15 CAGCTG C[AC]G^C[GT]G 424 CCGCTG PflMI 446 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 671 CCAAACTGTGG PleI 460 GACTC GAGTC(4/5) 997 GAGTC StyI 128 CCTTGG C^C[AT][AT]GG 143 CCATGG TaqI 356 TCGA T^CGA 458 TCGA TfiI 230 GAATC G^A[AT]TC 248 GATTC Tru9I 545 TTAA T^TAA 581 TTAA TseI 314 GCTGC G^C[AT]GC 801 GCAGC TthHB8I 356 TCGA T^CGA 458 TCGA Van91I 446 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 671 CCAAACTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AciI AfaI AluI AspHI AvaII BanI BmyI BsaJI BsiHKAI BsiYI BslI Bsp1286I BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EcoRII FauI Fnu4HI FokI HaeIII HinfI ItaI MboI MnlI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SspI Tsp509I