(Circular) (Six-base) MAPSORT of: pFN21AE6369.seq Check: 1605 from: 1 to: 5749 pFN21AE6369 5749 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3559 278 Size: 53 83 186 936 2023 2468 Fragments arranged by size: 2468 2023 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2463 2752 3092 1937 Size: 526 289 340 4594 Fragments arranged by size: 4594 526 340 289 Acc65I G'GTAC_C Cuts at: 2065 3070 2065 Size: 1005 4744 AclI AA'CG_TT Cuts at: 1563 3876 4249 1563 Size: 2313 373 3063 Fragments arranged by size: 3063 2313 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3812 5196 5464 1632 Size: 2180 1384 268 1917 Fragments arranged by size: 2180 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5552 AflIII A'CryG_T Cuts at: 1203 4648 5625 1203 Size: 3445 977 1327 Fragments arranged by size: 3445 1327 977 AgeI A'CCGG_T Cuts at: 4580 4580 Size: 5749 AhdI GACnn_n'nnGTC Cuts at: 4478 4478 Size: 5749 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5749 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2059 1184 Size: 875 4874 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2686 2718 2686 Size: 32 5717 AlwNI CAG_nnn'CTG Cuts at: 3492 5064 3492 Size: 1572 4177 ApaLI G'TGCA_C Cuts at: 3806 4962 3806 Size: 1156 4593 ApoI r'AATT_y Cuts at: 1493 2174 2967 3058 3284 3436 5545 1493 Size: 681 793 91 226 152 2109 1697 Fragments arranged by size: 2109 1697 793 681 226 152 91 AseI AT'TA_AT Cuts at: 160 1032 4303 160 Size: 872 3271 1606 Fragments arranged by size: 3271 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5749 AvaI C'yCGr_G Cuts at: 1870 1943 3074 1870 Size: 73 1131 4545 Fragments arranged by size: 4545 1131 73 BamHI G'GATC_C Cuts at: 3079 3446 3079 Size: 367 5382 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2065 2606 2766 3070 4525 Size: 325 217 627 278 541 160 304 1455 Cuts at: 4525 618 Size: 1842 Fragments arranged by size: 1842 1455 627 541 325 304 278 217 160 BanII G_rGCy'C Cuts at: 1391 2853 3068 1391 Size: 1462 215 4072 Fragments arranged by size: 4072 1462 215 BbeI G_GCGC'C Cuts at: 2610 2610 Size: 5749 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4778 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3940 3974 1903 Size: 34 2003 34 3678 Fragments arranged by size: 3678 2003 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3641 4851 3641 Size: 1210 4539 BclI T'GATC_A Cuts at: 1548 1548 Size: 5749 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4360 136 Size: 107 122 71 631 605 2688 1525 Fragments arranged by size: 2688 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5744 5744 Size: 5749 BlpI GC'TnA_GC Cuts at: 3166 3166 Size: 5749 Bme1580I G_kGCm'C Cuts at: 1790 2187 3810 4966 1790 Size: 397 1623 1156 2573 Fragments arranged by size: 2573 1623 1156 397 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5749 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3480 4438 442 Size: 416 53 83 399 24 2063 958 1753 Fragments arranged by size: 2063 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5749 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2273 2346 4409 1239 Size: 1034 73 2063 2579 Fragments arranged by size: 2579 2063 1034 73 Bpu10I CC'TnA_GC Cuts at: 2378 2378 Size: 5749 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3874 4739 5037 3874 Size: 865 298 4586 Fragments arranged by size: 4586 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4412 882 Size: 617 2913 2219 Fragments arranged by size: 2913 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3540 493 Size: 711 2336 2702 Fragments arranged by size: 2702 2336 711 BsaBI GATnn'nnATC Cuts at: 5743 5743 Size: 5749 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2607 3556 3938 4573 Size: 53 83 186 936 1074 949 382 635 Cuts at: 4573 275 Size: 1451 Fragments arranged by size: 1451 1074 949 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4182 4580 4854 5001 5412 4182 Size: 398 274 147 411 4519 Fragments arranged by size: 4519 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2987 3017 1178 Size: 30 165 30 1584 30 3910 Fragments arranged by size: 3910 1584 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2861 1186 Size: 1675 4074 BseYI C'CCAG_C Cuts at: 4952 5448 4952 Size: 496 5253 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5176 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2198 3199 3960 4988 1540 Size: 275 186 197 1001 761 1028 2301 Fragments arranged by size: 2301 1028 1001 761 275 197 186 BsiHKAI G_wGCw'C Cuts at: 2307 2625 2853 3810 3895 4966 2307 Size: 318 228 957 85 1071 3090 Fragments arranged by size: 3090 1071 957 318 228 85 BsmI GAATG_Cn' Cuts at: 3260 3353 3260 Size: 93 5656 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2187 2307 2625 2853 3068 3810 3895 Size: 399 397 120 318 228 215 742 85 Cuts at: 3895 4966 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 397 318 228 215 120 85 BspEI T'CCGG_A Cuts at: 5412 5412 Size: 5749 BspHI T'CATG_A Cuts at: 3638 5368 3638 Size: 1730 4019 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3104 844 Size: 2260 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2287 2875 3636 1394 Size: 429 464 588 761 3507 Fragments arranged by size: 3507 761 588 464 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2667 4244 4418 65 Size: 1183 1419 1577 174 1396 Fragments arranged by size: 1577 1419 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4393 4580 1700 Size: 110 2583 187 2869 Fragments arranged by size: 2869 2583 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5749 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5749 BssSI C'ACGA_G Cuts at: 3809 4821 3809 Size: 1012 4737 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5749 BstBI TT'CG_AA Cuts at: 4553 4553 Size: 5749 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5749 BstXI CCAn_nnnn'nTGG Cuts at: 2128 2347 2128 Size: 219 5530 BstYI r'GATC_y Cuts at: 1972 3079 3446 3831 3848 5300 5386 5398 5744 Size: 1107 367 385 17 1452 86 12 346 Cuts at: 5744 1972 Size: 1977 Fragments arranged by size: 1977 1452 1107 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2844 2844 Size: 5749 BtgI C'CryG_G Cuts at: 513 1065 2148 2198 513 Size: 552 1083 50 4064 Fragments arranged by size: 4064 1083 552 50 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2584 487 Size: 683 56 1358 3652 Fragments arranged by size: 3652 1358 683 56 BtsI GCAGTG_nn' Cuts at: 2405 3265 4059 4079 2405 Size: 860 794 20 4075 Fragments arranged by size: 4075 860 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5749 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2392 2427 529 Size: 35 1828 35 3851 Fragments arranged by size: 3851 1828 35 35 DraI TTT'AAA Cuts at: 3053 3053 Size: 5749 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2381 4756 809 Size: 507 1065 2375 1802 Fragments arranged by size: 2375 1802 1065 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2195 3196 4085 Size: 54 1425 50 275 18 365 1001 889 Cuts at: 4085 5714 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1001 889 365 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2195 3196 1537 Size: 275 383 1001 4090 Fragments arranged by size: 4090 1001 383 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3679 1353 Size: 2326 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4336 4710 4856 387 Size: 1064 138 2747 374 146 1280 Fragments arranged by size: 2747 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2851 2851 Size: 5749 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2273 2346 3812 4409 5196 5464 1239 Size: 393 641 73 1466 597 787 268 1524 Fragments arranged by size: 1524 1466 787 641 597 393 268 73 EcoNI CCTnn'n_nnAGG Cuts at: 2761 2761 Size: 5749 EcoRI G'AATT_C Cuts at: 3058 3436 3058 Size: 378 5371 FspI TGC'GCA Cuts at: 4255 4255 Size: 5749 HaeII r_GCGC'y Cuts at: 1678 2610 2661 4896 1678 Size: 932 51 2235 2531 Fragments arranged by size: 2531 2235 932 51 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2656 2688 2987 3019 4390 Size: 32 135 32 1084 32 299 32 1371 Cuts at: 4390 4422 4464 4496 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1371 1084 299 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2464 3093 3339 669 Size: 988 281 526 629 246 3079 Fragments arranged by size: 3079 988 629 526 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5749 HpaI GTT'AAC Cuts at: 3339 3339 Size: 5749 KasI G'GCGC_C Cuts at: 2606 2606 Size: 5749 KpnI G_GTAC'C Cuts at: 2069 3074 2069 Size: 1005 4744 MfeI C'AATT_G Cuts at: 3348 3348 Size: 5749 MluI A'CGCG_T Cuts at: 5625 5625 Size: 5749 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2617 4863 5047 1305 Size: 1312 2246 184 2007 Fragments arranged by size: 2246 2007 1312 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3927 Fragments arranged by size: 3927 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2540 2630 3707 4066 4225 518 Size: 648 1374 90 1077 359 159 2042 Fragments arranged by size: 2042 1374 1077 648 359 159 90 MspA1I CmG'CkG Cuts at: 1606 2022 2431 3165 3842 4990 5235 1606 Size: 416 409 734 677 1148 245 2120 Fragments arranged by size: 2120 1148 734 677 416 409 245 NarI GG'CG_CC Cuts at: 2607 2607 Size: 5749 NcoI C'CATG_G Cuts at: 513 1065 2148 513 Size: 552 1083 4114 Fragments arranged by size: 4114 1083 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5749 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5749 NotI GC'GGCC_GC Cuts at: 3196 3196 Size: 5749 NspI r_CATG'y Cuts at: 1132 1229 2400 3107 4652 1132 Size: 97 1171 707 1545 2229 Fragments arranged by size: 2229 1545 1171 707 97 PasI CC'CwG_GG Cuts at: 1348 2722 1348 Size: 1374 4375 PciI A'CATG_T Cuts at: 4648 4648 Size: 5749 PflMI CCAn_nnn'nTGG Cuts at: 1108 2457 2682 1108 Size: 1349 225 4175 Fragments arranged by size: 4175 1349 225 PmeI GTTT'AAAC Cuts at: 3053 3053 Size: 5749 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3818 3850 5331 5363 3818 Size: 32 1481 32 4204 Fragments arranged by size: 4204 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5749 PsiI TTA'TAA Cuts at: 3015 3319 3015 Size: 304 5445 PstI C_TGCA'G Cuts at: 830 3101 830 Size: 2271 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5749 PvuII CAG'CTG Cuts at: 2022 2022 Size: 5749 SacI G_AGCT'C Cuts at: 2853 2853 Size: 5749 SalI G'TCGA_C Cuts at: 1936 2462 3091 1936 Size: 526 629 4594 Fragments arranged by size: 4594 629 526 SbfI CC_TGCA'GG Cuts at: 3101 3101 Size: 5749 ScaI AGT'ACT Cuts at: 3997 5709 3997 Size: 1712 4037 SfcI C'TryA_G Cuts at: 826 1045 2881 3097 4232 4913 5104 826 Size: 219 1836 216 1135 681 191 1471 Fragments arranged by size: 1836 1471 1135 681 219 216 191 SfoI GGC'GCC Cuts at: 2608 2608 Size: 5749 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5749 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5749 SmaI CCC'GGG Cuts at: 1872 3076 1872 Size: 1204 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3853 4754 5016 820 Size: 197 926 1910 901 262 1553 Fragments arranged by size: 1910 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3540 493 Size: 3047 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5749 SphI G_CATG'C Cuts at: 1132 3107 1132 Size: 1975 3774 SspI AAT'ATT Cuts at: 5 52 2646 2700 2913 3673 5 Size: 47 2594 54 213 760 2081 Fragments arranged by size: 2594 2081 760 213 54 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5749 StyI C'CwwG_G Cuts at: 513 1065 2133 2148 3188 513 Size: 552 1068 15 1040 3074 Fragments arranged by size: 3074 1068 1040 552 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3776 3793 3946 4131 4576 325 Size: 1255 2196 17 153 185 445 1498 Fragments arranged by size: 2196 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3995 5707 96 Size: 274 80 33 51 1443 2018 1712 138 Fragments arranged by size: 2018 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2145 2177 2913 2945 5709 5741 2145 Size: 32 736 32 2764 32 2153 Fragments arranged by size: 2764 2153 736 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5749 XbaI T'CTAG_A Cuts at: 3085 3085 Size: 5749 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5749 XmaI C'CCGG_G Cuts at: 1870 3074 1870 Size: 1204 4545 XmnI GAAnn'nnTTC Cuts at: 3878 3878 Size: 5749 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3557 276 Size: 53 83 186 936 2023 2468 Fragments arranged by size: 2468 2023 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI