################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AviII AvrII BalI BamHI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaWI BseAI BsiWI BspDI BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII Eam1105I Eco47III Eco81I EcoNI EcoO65I FauI FbaI FseI FspI HincII HindII HpaI KpnI MaeI MfeI MluI MluNI MroI MscI MseI MslI MunI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI SspI StuI SwaI Tru9I Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AlwNI 1356 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 256 GGGCCC GGGCC^C BanI 109 GGCGCC G^G[CT][GA]CC BanII 256 GGGCCC G[GA]GC[CT]^C BbeI 109 GGCGCC GGCGC^C BbrPI 229 CACGTG CAC^GTG BbsI 1105 GAAGAC GAAGAC(2/6) BbvCI 958 GCTGAGG CCTCAGC(-5/-2) BcgI 245 GCATGTTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpuAI 1105 GAAGAC GAAGAC(2/6) BsaAI 229 CACGTG [CT]AC^GT[GA] BsaBI 760 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 192 CGGCCG CG[GA][CT]^CG BsmBI 695 CGTCTC CGTCTC(1/5) Bsp1407I 1387 TGTACA T^GTACA BsrGI 1387 TGTACA T^GTACA BstYI 993 GGATCT [GA]^GATC[CT] DraII 256 GGGCCCC [GA]G^G[ACGT]CC[CT] DrdI 208 GACTGCGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 192 CGGCCG C^GGCCG EarI 1315 GAAGAG CTCTTC(1/4) EclXI 192 CGGCCG C^GGCCG Eco52I 192 CGGCCG C^GGCCG EcoO109I 256 GGGCCCC [GA]G^G[ACGT]CC[CT] EcoRV 661 GATATC GAT^ATC HaeII 109 GGCGCC [GA]GCGC^[CT] HindIII 78 AAGCTT A^AGCTT KasI 109 GGCGCC G^GCGCC Ksp632I 1315 GAAGAG CTCTTC(1/4) KspI 1201 CCGCGG CCGC^GG MamI 760 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 993 GGATCT [GA]^GATC[CT] NarI 109 GGCGCC GG^CGCC PleI 136 GAGTC GAGTC(4/5) PmaCI 229 CACGTG CAC^GTG PmlI 229 CACGTG CAC^GTG SacII 1201 CCGCGG CCGC^GG SbfI 116 CCTGCAGG CCTGCA^GG SfoI 109 GGCGCC GGC^GCC Sse8387I 116 CCTGCAGG CCTGCA^GG SspBI 1387 TGTACA T^GTACA TaqI 1112 TCGA T^CGA TthHB8I 1112 TCGA T^CGA XcmI 354 CCAGGGATGGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 993 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AflIII 466 ACATGT A^C[GA][CT]GT 1141 ACATGT AlwI 766 GATCC GGATC(4/5) 993 GGATC BpmI 698 CTCCAG CTGGAG(16/14) 1049 CTCCAG BsaI 289 GAGACC GGTCTC(1/5) 1378 GGTCTC BseRI 96 GAGGAG GAGGAG(10/8) 961 GAGGAG BsgI 945 CTGCAC GTGCAG(16/14) 311 GTGCAG BsmI 550 GAATGC GAATGC(1/-1) 1423 GAATGC BspLU11I 466 ACATGT A^CATGT 1141 ACATGT BstAPI 707 GCAAAATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1259 GCATATTGTGC EaeI 103 TGGCCG [CT]^GGCC[GA] 192 CGGCCG EcoRI 432 GAATTC G^AATTC 649 GAATTC EcoT14I 318 CCATGG C^C[AT][AT]GG 588 CCATGG EcoT22I 552 ATGCAT ATGCA^T 1448 ATGCAT HphI 742 GGTGA GGTGA(8/7) 665 TCACC MaeII 230 ACGT A^CGT 694 ACGT NcoI 318 CCATGG C^CATGG 588 CCATGG NsiI 552 ATGCAT ATGCA^T 1448 ATGCAT PstI 117 CTGCAG CTGCA^G 714 CTGCAG PvuII 65 CAGCTG CAG^CTG 956 CAGCTG SmaI 128 CCCGGG CCC^GGG 171 CCCGGG StyI 318 CCATGG C^C[AT][AT]GG 588 CCATGG TfiI 1236 GAATC G^A[AT]TC 1246 GAATC XmaCI 128 CCCGGG C^CCGGG 171 CCCGGG XmaI 128 CCCGGG C^CCGGG 171 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI ApoI AspHI AvaI BbvI BcnI BmyI BsaHI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DsaI EcoRII Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII ItaI MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TseI Tsp45I Tsp509I TspRI