(Circular) (Six-base) MAPSORT of: pFN21AB5592.seq Check: 4875 from: 1 to: 6187 pFN21AB5592 6187 bp With 182 enzymes: SgfI * June 18, 2013 12:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2475 3053 2475 Size: 578 5609 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3997 278 Size: 53 83 186 936 2461 2468 Fragments arranged by size: 2468 2461 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3530 1937 Size: 1593 4594 Acc65I G'GTAC_C Cuts at: 3508 3508 Size: 6187 AclI AA'CG_TT Cuts at: 1563 4314 4687 1563 Size: 2751 373 3063 Fragments arranged by size: 3063 2751 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2005 3339 4250 5634 5902 1632 Size: 373 1334 911 1384 268 1917 Fragments arranged by size: 1917 1384 1334 911 373 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5990 AflIII A'CryG_T Cuts at: 1203 2471 3146 5086 6063 1203 Size: 1268 675 1940 977 1327 Fragments arranged by size: 1940 1327 1268 977 675 AgeI A'CCGG_T Cuts at: 5018 5018 Size: 6187 AhdI GACnn_n'nnGTC Cuts at: 4916 4916 Size: 6187 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6187 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6187 AlwNI CAG_nnn'CTG Cuts at: 3366 3930 5502 3366 Size: 564 1572 4051 Fragments arranged by size: 4051 1572 564 ApaI G_GGCC'C Cuts at: 2265 2265 Size: 6187 ApaLI G'TGCA_C Cuts at: 4244 5400 4244 Size: 1156 5031 ApoI r'AATT_y Cuts at: 1493 2437 2531 2654 3496 3722 3874 5983 1493 Size: 944 94 123 842 226 152 2109 1697 Fragments arranged by size: 2109 1697 944 842 226 152 123 94 AseI AT'TA_AT Cuts at: 160 1032 4741 160 Size: 872 3709 1606 Fragments arranged by size: 3709 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6187 AvaI C'yCGr_G Cuts at: 1870 1943 2133 2176 3274 3512 1870 Size: 73 190 43 1098 238 4545 Fragments arranged by size: 4545 1098 238 190 73 43 BamHI G'GATC_C Cuts at: 3517 3884 3517 Size: 367 5820 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2114 3508 4963 618 Size: 325 217 627 327 1394 1455 1842 Fragments arranged by size: 1842 1455 1394 627 327 325 217 BanII G_rGCy'C Cuts at: 1391 2265 3506 1391 Size: 874 1241 4072 Fragments arranged by size: 4072 1241 874 BbeI G_GCGC'C Cuts at: 2118 2118 Size: 6187 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3117 928 Size: 971 1218 3998 Fragments arranged by size: 3998 1218 971 BbvCI CC'TCA_GC Cuts at: 2964 2964 Size: 6187 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2239 2273 4378 4412 1903 Size: 34 50 34 218 34 2105 34 3678 Fragments arranged by size: 3678 2105 218 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4079 5289 4079 Size: 1210 4977 BclI T'GATC_A Cuts at: 1548 1548 Size: 6187 BfrBI ATG'CAT Cuts at: 2559 3455 2559 Size: 896 5291 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4798 136 Size: 107 122 71 631 605 3126 1525 Fragments arranged by size: 3126 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6182 6182 Size: 6187 BlpI GC'TnA_GC Cuts at: 3604 3604 Size: 6187 Bme1580I G_kGCm'C Cuts at: 1790 2265 4248 5404 1790 Size: 475 1983 1156 2573 Fragments arranged by size: 2573 1983 1156 475 BmgBI CAC'GTC Cuts at: 1318 2700 1318 Size: 1382 4805 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2787 2794 3918 Size: 416 53 83 399 24 1370 7 1124 Cuts at: 3918 4876 442 Size: 958 1753 Fragments arranged by size: 1753 1370 1124 958 416 399 83 53 24 7 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6187 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2688 3039 4847 1239 Size: 1449 351 1808 2579 Fragments arranged by size: 2579 1808 1449 351 Bpu10I CC'TnA_GC Cuts at: 2964 2964 Size: 6187 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4312 5177 5475 4312 Size: 865 298 5024 Fragments arranged by size: 5024 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2288 3389 4850 882 Size: 617 789 1101 1461 2219 Fragments arranged by size: 2219 1461 1101 789 617 BsaAI yAC'GTr Cuts at: 493 1204 2236 3978 493 Size: 711 1032 1742 2702 Fragments arranged by size: 2702 1742 1032 711 BsaBI GATnn'nnATC Cuts at: 2769 6181 2769 Size: 3412 2775 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2042 2115 2220 3994 Size: 53 83 186 936 509 73 105 1774 Cuts at: 3994 4376 5011 275 Size: 382 635 1451 Fragments arranged by size: 1774 1451 936 635 509 382 186 105 83 73 53 BsaWI w'CCGG_w Cuts at: 4620 5018 5292 5439 5850 4620 Size: 398 274 147 411 4957 Fragments arranged by size: 4957 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2299 2329 1178 Size: 30 165 30 896 30 5036 Fragments arranged by size: 5036 896 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2116 2981 1186 Size: 930 865 4392 Fragments arranged by size: 4392 930 865 BseYI C'CCAG_C Cuts at: 2060 3432 5390 5886 2060 Size: 1372 1958 496 2361 Fragments arranged by size: 2361 1958 1372 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2337 2935 1163 Size: 573 601 598 4415 Fragments arranged by size: 4415 601 598 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2200 3637 4398 5426 1540 Size: 275 186 199 1437 761 1028 2301 Fragments arranged by size: 2301 1437 1028 761 275 199 186 BsiHKAI G_wGCw'C Cuts at: 2312 2940 3275 4248 4333 5404 2312 Size: 628 335 973 85 1071 3095 Fragments arranged by size: 3095 1071 973 628 335 85 BsmI GAATG_Cn' Cuts at: 2561 3434 3698 3791 2561 Size: 873 264 93 4957 Fragments arranged by size: 4957 873 264 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2706 2706 Size: 6187 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2265 2312 2940 3275 3506 4248 4333 Size: 399 475 47 628 335 231 742 85 Cuts at: 4333 5404 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 628 475 399 335 231 85 47 BspEI T'CCGG_A Cuts at: 5850 5850 Size: 6187 BspHI T'CATG_A Cuts at: 4076 5806 4076 Size: 1730 4457 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2456 2475 3053 3542 844 Size: 1612 19 578 489 3489 Fragments arranged by size: 3489 1612 578 489 19 BsrBI CCG'CTC Cuts at: 1394 1823 4074 1394 Size: 429 2251 3507 Fragments arranged by size: 3507 2251 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4682 4856 65 Size: 1183 3434 174 1396 Fragments arranged by size: 3434 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4831 5018 1700 Size: 110 3021 187 2869 Fragments arranged by size: 3021 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3392 96 Size: 3296 2891 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6187 BssSI C'ACGA_G Cuts at: 4247 5259 4247 Size: 1012 5175 BstAPI GCAn_nnn'nTGC Cuts at: 794 2718 3270 794 Size: 1924 552 3711 Fragments arranged by size: 3711 1924 552 BstBI TT'CG_AA Cuts at: 4991 4991 Size: 6187 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6187 BstYI r'GATC_y Cuts at: 1972 2998 3517 3884 4269 4286 5738 5824 5836 Size: 1026 519 367 385 17 1452 86 12 Cuts at: 5836 6182 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1026 519 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2194 2323 2593 3139 3206 513 Size: 552 1129 129 270 546 67 3494 Fragments arranged by size: 3494 1129 552 546 270 129 67 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5448 Fragments arranged by size: 5448 683 56 BtsI GCAGTG_nn' Cuts at: 3703 4497 4517 3703 Size: 794 20 5373 Fragments arranged by size: 5373 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6187 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6152 DraI TTT'AAA Cuts at: 3491 3491 Size: 6187 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2219 5194 809 Size: 507 903 2975 1802 Fragments arranged by size: 2975 1802 903 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2108 2197 3634 Size: 54 1425 50 275 18 278 89 1437 Cuts at: 3634 4523 6152 8 Size: 889 1629 43 Fragments arranged by size: 1629 1437 1425 889 278 275 89 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2197 3634 1537 Size: 275 385 1437 4090 Fragments arranged by size: 4090 1437 385 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3315 4117 1353 Size: 1962 802 3423 Fragments arranged by size: 3423 1962 802 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4774 5148 5294 387 Size: 1064 138 3185 374 146 1280 Fragments arranged by size: 3185 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2005 2688 3039 3339 4250 4847 5634 Size: 393 373 683 351 300 911 597 787 Cuts at: 5634 5902 1239 Size: 268 1524 Fragments arranged by size: 1524 911 787 683 597 393 373 351 300 268 EcoO109I rG'GnC_Cy Cuts at: 2262 2262 Size: 6187 EcoRI G'AATT_C Cuts at: 2437 2654 3496 3874 2437 Size: 217 842 378 4750 Fragments arranged by size: 4750 842 378 217 EcoRV GAT'ATC Cuts at: 2668 2668 Size: 6187 FspI TGC'GCA Cuts at: 4693 4693 Size: 6187 HaeII r_GCGC'y Cuts at: 1678 2118 5334 1678 Size: 440 3216 2531 Fragments arranged by size: 3216 2531 440 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4828 4860 4902 4934 1373 Size: 32 135 32 3256 32 42 32 2626 Fragments arranged by size: 3256 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3531 3777 669 Size: 988 281 1593 246 3079 Fragments arranged by size: 3079 1593 988 281 246 HindIII A'AGCT_T Cuts at: 748 2083 748 Size: 1335 4852 HpaI GTT'AAC Cuts at: 3777 3777 Size: 6187 KasI G'GCGC_C Cuts at: 2114 2114 Size: 6187 KpnI G_GTAC'C Cuts at: 3512 3512 Size: 6187 MfeI C'AATT_G Cuts at: 3786 3786 Size: 6187 MluI A'CGCG_T Cuts at: 6063 6063 Size: 6187 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2124 2798 5301 5485 1305 Size: 819 674 2503 184 2007 Fragments arranged by size: 2503 2007 819 674 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4365 Fragments arranged by size: 4365 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4145 4504 4663 518 Size: 648 2979 359 159 2042 Fragments arranged by size: 2979 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2072 2208 2963 3105 3208 3603 4280 5428 Size: 466 136 755 142 103 395 677 1148 Cuts at: 5428 5673 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 755 677 466 395 245 142 136 103 NarI GG'CG_CC Cuts at: 2115 2115 Size: 6187 NcoI C'CATG_G Cuts at: 513 1065 2323 2593 513 Size: 552 1258 270 4107 Fragments arranged by size: 4107 1258 552 270 NdeI CA'TA_TG Cuts at: 387 387 Size: 6187 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6187 NotI GC'GGCC_GC Cuts at: 3634 3634 Size: 6187 NsiI A_TGCA'T Cuts at: 2561 3457 2561 Size: 896 5291 NspI r_CATG'y Cuts at: 1132 1229 2254 2475 3150 3459 3545 5090 1132 Size: 97 1025 221 675 309 86 1545 2229 Fragments arranged by size: 2229 1545 1025 675 309 221 97 86 PasI CC'CwG_GG Cuts at: 1348 2676 1348 Size: 1328 4859 PciI A'CATG_T Cuts at: 2471 3146 5086 2471 Size: 675 1940 3572 Fragments arranged by size: 3572 1940 675 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6187 PfoI T'CCnGG_A Cuts at: 3055 3055 Size: 6187 PmeI GTTT'AAAC Cuts at: 3491 3491 Size: 6187 PmlI CAC'GTG Cuts at: 2236 2236 Size: 6187 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4256 4288 5769 5801 4256 Size: 32 1481 32 4642 Fragments arranged by size: 4642 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6187 PsiI TTA'TAA Cuts at: 3757 3757 Size: 6187 PspOMI G'GGCC_C Cuts at: 2261 2261 Size: 6187 PstI C_TGCA'G Cuts at: 830 2126 2723 3539 830 Size: 1296 597 816 3478 Fragments arranged by size: 3478 1296 816 597 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6187 PvuII CAG'CTG Cuts at: 2072 2963 2072 Size: 891 5296 SacII CC_GC'GG Cuts at: 3209 3209 Size: 6187 SalI G'TCGA_C Cuts at: 1936 3529 1936 Size: 1593 4594 SbfI CC_TGCA'GG Cuts at: 2126 3539 2126 Size: 1413 4774 ScaI AGT'ACT Cuts at: 4435 6147 4435 Size: 1712 4475 SfcI C'TryA_G Cuts at: 826 1045 2122 2719 2830 3535 4670 5351 5542 Size: 219 1077 597 111 705 1135 681 191 Cuts at: 5542 826 Size: 1471 Fragments arranged by size: 1471 1135 1077 705 681 597 219 191 111 SfoI GGC'GCC Cuts at: 2116 2116 Size: 6187 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6187 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6187 SmaI CCC'GGG Cuts at: 1872 2135 2178 3514 1872 Size: 263 43 1336 4545 Fragments arranged by size: 4545 1336 263 43 SmlI C'TyrA_G Cuts at: 820 1017 1943 4291 5192 5454 820 Size: 197 926 2348 901 262 1553 Fragments arranged by size: 2348 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3978 493 Size: 3485 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6187 SphI G_CATG'C Cuts at: 1132 3545 1132 Size: 2413 3774 SspI AAT'ATT Cuts at: 5 52 4111 5 Size: 47 4059 2081 Fragments arranged by size: 4059 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6187 StyI C'CwwG_G Cuts at: 513 1065 2323 2593 3626 513 Size: 552 1258 270 1033 3074 Fragments arranged by size: 3074 1258 1033 552 270 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2275 2862 2955 4214 4231 4384 4569 Size: 1255 695 587 93 1259 17 153 185 Cuts at: 4569 5014 325 Size: 445 1498 Fragments arranged by size: 1498 1259 1255 695 587 445 185 153 93 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3392 4433 6145 Size: 274 80 33 51 1443 1415 1041 1712 Cuts at: 6145 96 Size: 138 Fragments arranged by size: 1712 1443 1415 1041 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2299 2331 2917 2930 2949 2962 3034 3066 6147 Size: 32 586 13 19 13 72 32 3081 Cuts at: 6147 6179 2299 Size: 32 2307 Fragments arranged by size: 3081 2307 586 72 32 32 32 19 13 13 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6187 XbaI T'CTAG_A Cuts at: 3523 3523 Size: 6187 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2366 2366 Size: 6187 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6187 XmaI C'CCGG_G Cuts at: 1870 2133 2176 3512 1870 Size: 263 43 1336 4545 Fragments arranged by size: 4545 1336 263 43 XmnI GAAnn'nnTTC Cuts at: 4316 4316 Size: 6187 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3995 276 Size: 53 83 186 936 2461 2468 Fragments arranged by size: 2468 2461 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI NaeI NgoMIV NruI PacI PpuMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI