################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI AocI Aor51HI AscI Asp700I Asp718I AspI AviII AvrII BamHI BbeI BbvCI BcgI BciVI BfrI BglII BlnI BlpI Bpu1102I BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BspEI BspHI BspLU11I BsrFI BssHII Bst1107I BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I CpoI DraI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV FseI FspI HaeII HhaI HinP1I KasI KpnI KspI MflI MluI MroI NaeI NarI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 68 enzymes) # ################################### AccI 268 GTCTAC GT^[AC][GT]AC AccII 1959 CGCG CG^CG AcyI 195 GACGCC G[GA]^CG[CT]C AhdI 281 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 358 GTGCAC G^TGCAC AlwI 1330 GGATC GGATC(4/5) AlwNI 1337 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 1394 GGGCCC GGGCC^C ApaLI 358 GTGCAC G^TGCAC AspEI 281 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 400 CTCGGG C^[CT]CG[GA]G BalI 707 TGGCCA TGG^CCA BanI 337 GGCACC G^G[CT][GA]CC BbrPI 361 CACGTG CAC^GTG BclI 1001 TGATCA T^GATCA BcnI 935 CCGGG CC^[GC]GG BfaI 1387 CTAG C^TAG BsaAI 361 CACGTG [CT]AC^GT[GA] BsaBI 996 GATCGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 195 GACGCC G[GA]^CG[CT]C BsgI 1132 GTGCAG GTGCAG(16/14) BsmAI 1263 GTCTC GTCTC(1/5) BsmI 95 GAATGC GAATGC(1/-1) BsoBI 400 CTCGGG C^[CT]CG[GA]G Bsp1407I 665 TGTACA T^GTACA BspDI 1144 ATCGAT AT^CGAT BspMI 1134 GCAGGT ACCTGC(4/8) BsrBI 811 GAGCGG CCGCTC(-3/-3) BsrDI 1041 CATTGC GCAATG(2/0) BsrGI 665 TGTACA T^GTACA BssSI 225 CTCGTG CACGAG(-5/-1) BstAPI 360 GCACGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 1959 CGCG CG^CG BstXI 871 CCAATGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ClaI 1144 ATCGAT AT^CGAT DraII 1394 GGGCCCC [GA]G^G[ACGT]CC[CT] DraIII 361 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 246 GACCATTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 707 TGGCCA [CT]^GGCC[GA] Eam1105I 281 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 1394 GGGCCCC [GA]G^G[ACGT]CC[CT] EcoT22I 1274 ATGCAT ATGCA^T FauI 1755 CCCGC CCCGC(4/6) FbaI 1001 TGATCA T^GATCA HapII 935 CCGG C^CGG HgaI 195 GACGC GACGC(5/10) Hin1I 195 GACGCC G[GA]^CG[CT]C HindIII 584 AAGCTT A^AGCTT HpaI 1735 GTTAAC GTT^AAC HpaII 935 CCGG C^CGG MaeI 1387 CTAG C^TAG MamI 996 GATCGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 275 CAATTG C^AATTG MluNI 707 TGGCCA TGG^CCA MscI 707 TGGCCA TGG^CCA MspI 935 CCGG C^CGG MunI 275 CAATTG C^AATTG MvnI 1959 CGCG CG^CG NciI 935 CCGGG CC^[GC]GG NcoI 1106 CCATGG C^CATGG NsiI 1274 ATGCAT ATGCA^T PleI 223 GACTC GAGTC(4/5) PmaCI 361 CACGTG CAC^GTG PmlI 361 CACGTG CAC^GTG PstI 916 CTGCAG CTGCA^G SexAI 1409 ACCAGGT A^CC[AT]GGT SfiI 1101 GGCCTCCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SspBI 665 TGTACA T^GTACA ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AseI 307 ATTAAT AT^TAAT 508 ATTAAT AsnI 307 ATTAAT AT^TAAT 508 ATTAAT AvaII 1008 GGTCC G^G[AT]CC 1095 GGTCC BbsI 1162 GTCTTC GAAGAC(2/6) 1789 GTCTTC BglI 1102 GCCTCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1990 GCCATCATGGC BpuAI 1162 GTCTTC GAAGAC(2/6) 1789 GTCTTC BsmFI 403 GGGAC GGGAC(10/14) 1009 GTCCC DsaI 1106 CCATGG C^C[GA][CT]GG 1874 CCGTGG EcoRI 569 GAATTC G^AATTC 1810 GAATTC EcoT14I 651 CCTTGG C^C[AT][AT]GG 1106 CCATGG HincII 1616 GTCAAC GT[CT]^[GA]AC 1735 GTTAAC HindII 1616 GTCAAC GT[CT]^[GA]AC 1735 GTTAAC MaeII 172 ACGT A^CGT 362 ACGT PshBI 307 ATTAAT AT^TAAT 508 ATTAAT PvuII 135 CAGCTG CAG^CTG 1689 CAGCTG SacI 209 GAGCTC GAGCT^C 1486 GAGCTC SfcI 629 CTGTAG C^T[GA][CT]AG 916 CTGCAG StyI 651 CCTTGG C^C[AT][AT]GG 1106 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI AspHI BanII BbvI BmyI BpmI BsaJI BsiHKAI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeIII MboI MboII MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI