(Circular) (Six-base) MAPSORT of: pFN21AE5751.seq Check: 1231 from: 1 to: 6829 pFN21AE5751 6829 bp With 182 enzymes: SgfI * December 8, 2014 13:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3130 3130 Size: 6829 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4639 278 Size: 53 83 186 936 3103 2468 Fragments arranged by size: 3103 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2274 4172 1937 Size: 337 1898 4594 Fragments arranged by size: 4594 1898 337 Acc65I G'GTAC_C Cuts at: 4150 4150 Size: 6829 AclI AA'CG_TT Cuts at: 1563 4956 5329 1563 Size: 3393 373 3063 Fragments arranged by size: 3393 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3723 3842 4892 6276 6544 1632 Size: 2091 119 1050 1384 268 1917 Fragments arranged by size: 2091 1917 1384 1050 268 119 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6632 AflIII A'CryG_T Cuts at: 1203 5728 6705 1203 Size: 4525 977 1327 Fragments arranged by size: 4525 1327 977 AgeI A'CCGG_T Cuts at: 5660 5660 Size: 6829 AhdI GACnn_n'nnGTC Cuts at: 2291 5558 2291 Size: 3267 3562 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6829 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6829 AlwNI CAG_nnn'CTG Cuts at: 3347 4572 6144 3347 Size: 1225 1572 4032 Fragments arranged by size: 4032 1572 1225 ApaI G_GGCC'C Cuts at: 3403 3403 Size: 6829 ApaLI G'TGCA_C Cuts at: 2363 4886 6042 2363 Size: 2523 1156 3150 Fragments arranged by size: 3150 2523 1156 ApoI r'AATT_y Cuts at: 1493 2131 2574 2961 3650 3815 4138 4364 4516 Size: 638 443 387 689 165 323 226 152 Cuts at: 4516 6625 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 689 638 443 387 323 226 165 152 AseI AT'TA_AT Cuts at: 160 1032 2313 2514 5383 160 Size: 872 1281 201 2869 1606 Fragments arranged by size: 2869 1606 1281 872 201 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6829 AvaI C'yCGr_G Cuts at: 1870 1943 2405 4154 1870 Size: 73 462 1749 4545 Fragments arranged by size: 4545 1749 462 73 BamHI G'GATC_C Cuts at: 4159 4526 4159 Size: 367 6462 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2342 4150 5605 618 Size: 325 217 627 555 1808 1455 1842 Fragments arranged by size: 1842 1808 1455 627 555 325 217 BanII G_rGCy'C Cuts at: 1391 2218 2909 3403 3495 4148 1391 Size: 827 691 494 92 653 4072 Fragments arranged by size: 4072 827 691 653 494 92 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3160 3787 928 Size: 971 1261 627 3970 Fragments arranged by size: 3970 1261 971 627 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5020 5054 1903 Size: 34 3083 34 3678 Fragments arranged by size: 3678 3083 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4721 5931 4721 Size: 1210 5619 BclI T'GATC_A Cuts at: 1548 3006 1548 Size: 1458 5371 BfrBI ATG'CAT Cuts at: 3281 3281 Size: 6829 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3113 4001 5440 Size: 107 122 71 631 605 1441 888 1439 Cuts at: 5440 136 Size: 1525 Fragments arranged by size: 1525 1441 1439 888 631 605 122 107 71 BglII A'GATC_T Cuts at: 6824 6824 Size: 6829 BlpI GC'TnA_GC Cuts at: 4246 4246 Size: 6829 Bme1580I G_kGCm'C Cuts at: 1790 2367 3403 4890 6046 1790 Size: 577 1036 1487 1156 2573 Fragments arranged by size: 2573 1487 1156 1036 577 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6829 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2803 4560 5518 Size: 416 53 83 399 24 1386 1757 958 Cuts at: 5518 442 Size: 1753 Fragments arranged by size: 1757 1753 1386 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6829 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2433 2893 3261 5489 1239 Size: 1194 460 368 2228 2579 Fragments arranged by size: 2579 2228 1194 460 368 Bpu10I CC'TnA_GC Cuts at: 2045 2045 Size: 6829 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4954 5819 6117 4954 Size: 865 298 5666 Fragments arranged by size: 5666 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5492 882 Size: 617 3993 2219 Fragments arranged by size: 3993 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2368 4620 493 Size: 711 1164 2252 2702 Fragments arranged by size: 2702 2252 1164 711 BsaBI GATnn'nnATC Cuts at: 3005 6823 3005 Size: 3818 3011 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2201 4636 5018 5653 Size: 53 83 186 936 668 2435 382 635 Cuts at: 5653 275 Size: 1451 Fragments arranged by size: 2435 1451 936 668 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5262 5660 5934 6081 6492 5262 Size: 398 274 147 411 5599 Fragments arranged by size: 5599 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3234 3264 1178 Size: 30 165 30 1831 30 4743 Fragments arranged by size: 4743 1831 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6829 BseYI C'CCAG_C Cuts at: 2142 2236 4027 6032 6528 2142 Size: 94 1791 2005 496 2443 Fragments arranged by size: 2443 2005 1791 496 94 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3158 1163 Size: 573 1422 4834 Fragments arranged by size: 4834 1422 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4279 5040 6068 1540 Size: 275 186 2278 761 1028 2301 Fragments arranged by size: 2301 2278 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2218 2367 2784 3495 3906 4890 4975 6046 2218 Size: 149 417 711 411 984 85 1071 3001 Fragments arranged by size: 3001 1071 984 711 417 411 149 85 BsmI GAATG_Cn' Cuts at: 2106 4340 4433 2106 Size: 2234 93 4502 Fragments arranged by size: 4502 2234 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2218 2367 2784 2909 3403 3495 3906 Size: 399 428 149 417 125 494 92 411 Cuts at: 3906 4148 4890 4975 6046 1391 Size: 242 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 494 428 417 411 399 242 149 125 92 85 BspEI T'CCGG_A Cuts at: 6492 6492 Size: 6829 BspHI T'CATG_A Cuts at: 4718 6448 4718 Size: 1730 5099 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3130 4184 844 Size: 2286 1054 3489 Fragments arranged by size: 3489 2286 1054 BsrBI CCG'CTC Cuts at: 1394 1823 2818 4716 1394 Size: 429 995 1898 3507 Fragments arranged by size: 3507 1898 995 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3045 5324 5498 65 Size: 1183 1797 2279 174 1396 Fragments arranged by size: 2279 1797 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5473 5660 1700 Size: 110 3663 187 2869 Fragments arranged by size: 3663 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2670 96 Size: 2574 4255 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6829 BssSI C'ACGA_G Cuts at: 2230 4889 5901 2230 Size: 2659 1012 3158 Fragments arranged by size: 3158 2659 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2371 794 Size: 1577 5252 BstBI TT'CG_AA Cuts at: 5633 5633 Size: 6829 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6829 BstXI CCAn_nnnn'nTGG Cuts at: 2883 2883 Size: 6829 BstYI r'GATC_y Cuts at: 1972 4159 4526 4911 4928 6380 6466 6478 6824 Size: 2187 367 385 17 1452 86 12 346 Cuts at: 6824 1972 Size: 1977 Fragments arranged by size: 2187 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3111 3879 513 Size: 552 939 1107 768 3463 Fragments arranged by size: 3463 1107 939 768 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6090 Fragments arranged by size: 6090 683 56 BtsI GCAGTG_nn' Cuts at: 2668 2918 3345 4345 5139 5159 2668 Size: 250 427 1000 794 20 4338 Fragments arranged by size: 4338 1000 794 427 250 20 ClaI AT'CG_AT Cuts at: 1554 3150 1554 Size: 1596 5233 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6794 DraI TTT'AAA Cuts at: 4133 4133 Size: 6829 DraIII CAC_nnn'GTG Cuts at: 2371 2371 Size: 6829 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2257 5836 809 Size: 507 941 3579 1802 Fragments arranged by size: 3579 1802 941 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2712 4276 5165 Size: 54 1425 50 275 18 882 1564 889 Cuts at: 5165 6794 8 Size: 1629 43 Fragments arranged by size: 1629 1564 1425 889 882 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4276 1537 Size: 275 2464 4090 Fragments arranged by size: 4090 2464 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2454 3165 3240 4759 1353 Size: 1101 711 75 1519 3423 Fragments arranged by size: 3423 1519 1101 711 75 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3600 5416 5790 5936 387 Size: 1064 138 2011 1816 374 146 1280 Fragments arranged by size: 2011 1816 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2216 3493 2216 Size: 1277 5552 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2433 2893 3261 3723 3842 4892 5489 Size: 393 801 460 368 462 119 1050 597 Cuts at: 5489 6276 6544 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1050 801 787 597 462 460 393 368 268 119 EcoO109I rG'GnC_Cy Cuts at: 3400 3400 Size: 6829 EcoRI G'AATT_C Cuts at: 2574 3815 4138 4516 2574 Size: 1241 323 378 4887 Fragments arranged by size: 4887 1241 378 323 FspI TGC'GCA Cuts at: 5335 5335 Size: 6829 HaeII r_GCGC'y Cuts at: 1678 5976 1678 Size: 4298 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2141 2173 2205 2237 2277 Size: 32 135 32 569 32 32 32 40 Cuts at: 2277 2309 3327 3359 4027 4059 5470 5502 5544 Size: 32 1018 32 668 32 1411 32 42 Cuts at: 5544 5576 1373 Size: 32 2626 Fragments arranged by size: 2626 1411 1018 668 569 135 42 40 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3623 3742 4173 4419 669 Size: 988 281 1685 119 431 246 3079 Fragments arranged by size: 3079 1685 988 431 281 246 119 HindIII A'AGCT_T Cuts at: 748 2589 748 Size: 1841 4988 HpaI GTT'AAC Cuts at: 3742 4419 3742 Size: 677 6152 KpnI G_GTAC'C Cuts at: 4154 4154 Size: 6829 MfeI C'AATT_G Cuts at: 2280 4428 2280 Size: 2148 4681 MluI A'CGCG_T Cuts at: 6705 6705 Size: 6829 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3864 5943 6127 1305 Size: 2559 2079 184 2007 Fragments arranged by size: 2559 2079 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2714 10 Size: 54 1425 343 882 4125 Fragments arranged by size: 4125 1425 882 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2334 2549 3506 3899 4787 5146 Size: 648 843 325 215 957 393 888 359 Cuts at: 5146 5305 518 Size: 159 2042 Fragments arranged by size: 2042 957 888 843 648 393 359 325 215 159 MspA1I CmG'CkG Cuts at: 1606 2142 3696 4031 4245 4922 6070 6315 1606 Size: 536 1554 335 214 677 1148 245 2120 Fragments arranged by size: 2120 1554 1148 677 536 335 245 214 NcoI C'CATG_G Cuts at: 513 1065 2004 3111 513 Size: 552 939 1107 4231 Fragments arranged by size: 4231 1107 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6829 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6829 NotI GC'GGCC_GC Cuts at: 4276 4276 Size: 6829 NsiI A_TGCA'T Cuts at: 3283 3283 Size: 6829 NspI r_CATG'y Cuts at: 1132 1229 4187 5732 1132 Size: 97 2958 1545 2229 Fragments arranged by size: 2958 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6829 PciI A'CATG_T Cuts at: 5728 5728 Size: 6829 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6829 PfoI T'CCnGG_A Cuts at: 2939 3238 3331 2939 Size: 299 93 6437 Fragments arranged by size: 6437 299 93 PmeI GTTT'AAAC Cuts at: 4133 4133 Size: 6829 PmlI CAC'GTG Cuts at: 2368 2368 Size: 6829 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4898 4930 6411 6443 4898 Size: 32 1481 32 5284 Fragments arranged by size: 5284 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6829 PsiI TTA'TAA Cuts at: 4399 4399 Size: 6829 PspOMI G'GGCC_C Cuts at: 3399 3399 Size: 6829 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2663 2695 2663 Size: 32 6797 PstI C_TGCA'G Cuts at: 830 2925 4181 830 Size: 2095 1256 3478 Fragments arranged by size: 3478 2095 1256 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6829 PvuII CAG'CTG Cuts at: 2142 3696 2142 Size: 1554 5275 SacI G_AGCT'C Cuts at: 2218 3495 2218 Size: 1277 5552 SalI G'TCGA_C Cuts at: 1936 4171 1936 Size: 2235 4594 SbfI CC_TGCA'GG Cuts at: 4181 4181 Size: 6829 ScaI AGT'ACT Cuts at: 5077 6789 5077 Size: 1712 5117 SexAI A'CCwGG_T Cuts at: 3414 3414 Size: 6829 SfcI C'TryA_G Cuts at: 826 1045 2634 2921 4177 5312 5993 6184 826 Size: 219 1589 287 1256 1135 681 191 1471 Fragments arranged by size: 1589 1471 1256 1135 681 287 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3113 3113 Size: 6829 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6829 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6829 SmaI CCC'GGG Cuts at: 1872 4156 1872 Size: 2284 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4933 5834 6096 820 Size: 197 926 2990 901 262 1553 Fragments arranged by size: 2990 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4620 493 Size: 4127 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6829 SphI G_CATG'C Cuts at: 1132 4187 1132 Size: 3055 3774 SspI AAT'ATT Cuts at: 5 52 4753 5 Size: 47 4701 2081 Fragments arranged by size: 4701 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6829 StyI C'CwwG_G Cuts at: 513 1065 2004 2656 3111 4268 513 Size: 552 939 652 455 1157 3074 Fragments arranged by size: 3074 1157 939 652 552 455 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2563 4856 4873 5026 5211 5656 325 Size: 1255 983 2293 17 153 185 445 1498 Fragments arranged by size: 2293 1498 1255 983 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2121 2670 3948 Size: 274 80 33 51 1443 144 549 1278 Cuts at: 3948 5075 6787 96 Size: 1127 1712 138 Fragments arranged by size: 1712 1443 1278 1127 549 274 144 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3234 3266 6789 6821 3234 Size: 32 3523 32 3242 Fragments arranged by size: 3523 3242 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6829 XbaI T'CTAG_A Cuts at: 4165 4165 Size: 6829 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2336 2503 3118 3139 2336 Size: 167 615 21 6026 Fragments arranged by size: 6026 615 167 21 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6829 XmaI C'CCGG_G Cuts at: 1870 4154 1870 Size: 2284 4545 XmnI GAAnn'nnTTC Cuts at: 4958 4958 Size: 6829 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4637 276 Size: 53 83 186 936 3103 2468 Fragments arranged by size: 3103 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PpuMI PspXI RsrII SacII SanDI SapI SfoI SrfI SwaI