################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII AccI AccIII AcyI AflII AflIII AhdI Alw44I AlwI AlwNI AocI ApaI ApaLI AscI Asp700I AspEI AspI AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BseAI BseRI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eam1105I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI Hin1I HincII HindII HindIII HpaI KasI MamI MfeI MflI MluI MluNI MroI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NruI NsiI NspV PacI PaeR7I PflFI PflMI PleI PmaCI PmeI PmlI PshAI PvuI PvuII RcaI RsrII SacI SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Acc65I 865 GGTACC G^GTACC AclI 10 AACGTT AA^CGTT AgeI 480 ACCGGT A^CCGGT Aor51HI 1009 AGCGCT AGC^GCT AseI 241 ATTAAT AT^TAAT AsnI 241 ATTAAT AT^TAAT Asp718I 865 GGTACC G^GTACC AspHI 818 GTGCTC G[AT]GC[AT]^C AviII 738 TGCGCA TGC^GCA BanI 865 GGTACC G^G[CT][GA]CC BanII 495 GGGCTC G[GA]GC[CT]^C BcgI 614 GCACCATGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 769 GCCGCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaI 580 GAGACC GGTCTC(1/5) BsaWI 480 ACCGGT [AT]^CCGG[AT] BsgI 710 CTGCAC GTGCAG(16/14) BsiEI 785 CGGCCG CG[GA][CT]^CG BsiHKAI 818 GTGCTC G[AT]GC[AT]^C BsmI 97 GAATGC GAATGC(1/-1) BsrFI 480 ACCGGT [GA]^CCGG[CT] BstAPI 283 GCAATGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 794 CCACAGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 480 ACCGGT [GA]^CCGG[CT] DpnII 621 GATC ^GATC DraII 354 AGGTCCC [GA]G^G[ACGT]CC[CT] EagI 785 CGGCCG C^GGCCG EclXI 785 CGGCCG C^GGCCG Eco47III 1009 AGCGCT AGC^GCT Eco52I 785 CGGCCG C^GGCCG EcoO109I 354 AGGTCCC [GA]G^G[ACGT]CC[CT] FspI 738 TGCGCA TGC^GCA HaeII 1009 AGCGCT [GA]GCGC^[CT] HgaI 890 GCGTC GACGC(5/10) KpnI 865 GGTACC GGTAC^C MboI 621 GATC ^GATC MslI 795 CACAGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeII 621 GATC ^GATC NotI 784 GCGGCCGC GC^GGCCGC NspI 76 ACATGC [GA]CATG^[CT] PinAI 480 ACCGGT A^CCGGT PpuMI 354 AGGTCCC [GA]G^G[AT]CC[CT] PshBI 241 ATTAAT AT^TAAT Psp1406I 10 AACGTT AA^CGTT SapI 820 GCTCTTC GCTCTTC(1/4) Sau3AI 621 GATC ^GATC SspI 695 AATATT AAT^ATT ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcsI 103 AAATTT [GA]^AATT[CT] 440 AAATTC AfaI 866 GTAC GT^AC 994 GTAC ApoI 103 AAATTT [GA]^AATT[CT] 440 AAATTC AvaI 492 CCCGGG C^[CT]CG[GA]G 572 CCCGGG AvaII 355 GGTCC G^G[AT]CC 429 GGTCC BfaI 235 CTAG C^TAG 400 CTAG BpmI 416 CTGGAG CTGGAG(16/14) 535 CTGGAG BsoBI 492 CCCGGG C^[CT]CG[GA]G 572 CCCGGG BsrDI 283 GCAATG GCAATG(2/0) 860 GCAATG DdeI 509 CTCAG C^T[ACGT]AG 520 CTCAG DsaI 782 CCGCGG C^C[GA][CT]GG 788 CCGCGG EarI 821 CTCTTC CTCTTC(1/4) 143 GAAGAG HinfI 163 GATTC G^A[ACGT]TC 261 GATTC Ksp632I 821 CTCTTC CTCTTC(1/4) 143 GAAGAG KspI 782 CCGCGG CCGC^GG 788 CCGCGG MaeI 235 CTAG C^TAG 400 CTAG MaeII 11 ACGT A^CGT 1015 ACGT MspA1I 782 CCGCGG C[AC]G^C[GT]G 788 CCGCGG RsaI 866 GTAC GT^AC 994 GTAC SacII 782 CCGCGG CCGC^GG 788 CCGCGG SfaNI 300 GATGC GCATC(5/9) 1002 GATGC SmaI 492 CCCGGG CCC^GGG 572 CCCGGG TaqI 184 TCGA T^CGA 623 TCGA TfiI 163 GATTC G^A[AT]TC 261 GATTC Tsp45I 484 GTCAC ^GT[GC]AC 632 GTGAC TthHB8I 184 TCGA T^CGA 623 TCGA XmaCI 492 CCCGGG C^CCGGG 572 CCCGGG XmaI 492 CCCGGG C^CCGGG 572 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AccII AciI AluI BbvI BcnI BmyI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I EaeI EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeIII MboII MnlI MseI MspI MvaI MvnI MwoI NciI NlaIII NlaIV PstI Sau96I ScrFI SfcI Tru9I TseI Tsp509I TspRI