(Circular) (Six-base) MAPSORT of: pFN21AE5555.seq Check: 6642 from: 1 to: 5806 pFN21AE5555 5806 bp With 182 enzymes: SgfI * January 30, 2014 17:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3616 278 Size: 53 83 186 936 2080 2468 Fragments arranged by size: 2468 2080 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3149 1937 Size: 1212 4594 Acc65I G'GTAC_C Cuts at: 2870 3127 2870 Size: 257 5549 AclI AA'CG_TT Cuts at: 1563 2016 3933 4306 1563 Size: 453 1917 373 3063 Fragments arranged by size: 3063 1917 453 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3869 5253 5521 1632 Size: 2237 1384 268 1917 Fragments arranged by size: 2237 1917 1384 268 AfeI AGC'GCT Cuts at: 3016 3016 Size: 5806 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5609 AflIII A'CryG_T Cuts at: 1203 4705 5682 1203 Size: 3502 977 1327 Fragments arranged by size: 3502 1327 977 AgeI A'CCGG_T Cuts at: 2485 4637 2485 Size: 2152 3654 AhdI GACnn_n'nnGTC Cuts at: 4535 4535 Size: 5806 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5806 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2295 1184 Size: 1111 4695 AlwNI CAG_nnn'CTG Cuts at: 3549 5121 3549 Size: 1572 4234 ApaLI G'TGCA_C Cuts at: 3863 5019 3863 Size: 1156 4650 ApoI r'AATT_y Cuts at: 1493 2108 2445 3115 3341 3493 5602 1493 Size: 615 337 670 226 152 2109 1697 Fragments arranged by size: 2109 1697 670 615 337 226 152 AseI AT'TA_AT Cuts at: 160 1032 2247 4360 160 Size: 872 1215 2113 1606 Fragments arranged by size: 2113 1606 1215 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5806 AvaI C'yCGr_G Cuts at: 1870 1943 2497 2577 3131 1870 Size: 73 554 80 554 4545 Fragments arranged by size: 4545 554 554 80 73 BamHI G'GATC_C Cuts at: 3136 3503 3136 Size: 367 5439 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2870 3127 4582 618 Size: 325 217 627 1083 257 1455 1842 Fragments arranged by size: 1842 1455 1083 627 325 257 217 BanII G_rGCy'C Cuts at: 1391 2504 3125 1391 Size: 1113 621 4072 Fragments arranged by size: 4072 1113 621 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4835 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2608 2642 3997 4031 1903 Size: 34 671 34 1355 34 3678 Fragments arranged by size: 3678 1355 671 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3698 4908 3698 Size: 1210 4596 BclI T'GATC_A Cuts at: 1548 1548 Size: 5806 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2780 4417 136 Size: 107 122 71 631 605 1108 1637 1525 Fragments arranged by size: 1637 1525 1108 631 605 122 107 71 BglII A'GATC_T Cuts at: 5801 5801 Size: 5806 BlpI GC'TnA_GC Cuts at: 3223 3223 Size: 5806 Bme1580I G_kGCm'C Cuts at: 1790 2346 2578 3867 5023 1790 Size: 556 232 1289 1156 2573 Fragments arranged by size: 2573 1289 1156 556 232 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5806 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2018 3537 4495 Size: 416 53 83 399 24 601 1519 958 Cuts at: 4495 442 Size: 1753 Fragments arranged by size: 1753 1519 958 601 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5806 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2442 2561 4466 1239 Size: 1203 119 1905 2579 Fragments arranged by size: 2579 1905 1203 119 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3931 4796 5094 3931 Size: 865 298 4643 Fragments arranged by size: 4643 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2579 4469 882 Size: 617 1080 1890 2219 Fragments arranged by size: 2219 1890 1080 617 BsaAI yAC'GTr Cuts at: 493 1204 3597 493 Size: 711 2393 2702 Fragments arranged by size: 2702 2393 711 BsaBI GATnn'nnATC Cuts at: 5800 5800 Size: 5806 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3613 3995 4630 275 Size: 53 83 186 936 2080 382 635 1451 Fragments arranged by size: 2080 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2485 4239 4637 4911 5058 5469 2485 Size: 1754 398 274 147 411 2822 Fragments arranged by size: 2822 1754 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5581 Fragments arranged by size: 5581 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5806 BseYI C'CCAG_C Cuts at: 2298 2891 5009 5505 2298 Size: 593 2118 496 2599 Fragments arranged by size: 2599 2118 593 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2700 1163 Size: 573 964 4269 Fragments arranged by size: 4269 964 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2793 3256 4017 5045 1540 Size: 275 186 792 463 761 1028 2301 Fragments arranged by size: 2301 1028 792 761 463 275 186 BsiHKAI G_wGCw'C Cuts at: 2827 3867 3952 5023 2827 Size: 1040 85 1071 3610 Fragments arranged by size: 3610 1071 1040 85 BsmI GAATG_Cn' Cuts at: 2108 3317 3410 2108 Size: 1209 93 4504 Fragments arranged by size: 4504 1209 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2346 2504 2578 2827 3125 3867 3952 Size: 399 556 158 74 249 298 742 85 Cuts at: 3952 5023 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 556 399 298 249 158 85 74 BspEI T'CCGG_A Cuts at: 5469 5469 Size: 5806 BspHI T'CATG_A Cuts at: 3695 5425 3695 Size: 1730 4076 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3161 844 Size: 2317 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3693 1394 Size: 429 1870 3507 Fragments arranged by size: 3507 1870 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2295 2872 4301 4475 65 Size: 1183 1047 577 1429 174 1396 Fragments arranged by size: 1429 1396 1183 1047 577 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2485 4450 4637 1700 Size: 110 675 1965 187 2869 Fragments arranged by size: 2869 1965 675 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5806 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5806 BssSI C'ACGA_G Cuts at: 3866 4878 3866 Size: 1012 4794 BstAPI GCAn_nnn'nTGC Cuts at: 794 2294 794 Size: 1500 4306 BstBI TT'CG_AA Cuts at: 4610 4610 Size: 5806 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5806 BstXI CCAn_nnnn'nTGG Cuts at: 2806 2806 Size: 5806 BstYI r'GATC_y Cuts at: 1972 3136 3503 3888 3905 5357 5443 5455 5801 Size: 1164 367 385 17 1452 86 12 346 Cuts at: 5801 1972 Size: 1977 Fragments arranged by size: 1977 1452 1164 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2787 2793 513 Size: 552 939 783 6 3526 Fragments arranged by size: 3526 939 783 552 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5067 Fragments arranged by size: 5067 683 56 BtsI GCAGTG_nn' Cuts at: 3322 4116 4136 3322 Size: 794 20 4992 Fragments arranged by size: 4992 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5806 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5771 DraI TTT'AAA Cuts at: 3110 3110 Size: 5806 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4813 809 Size: 507 3497 1802 Fragments arranged by size: 3497 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2772 2790 2796 Size: 54 1425 50 275 18 942 18 6 Cuts at: 2796 3253 4142 5771 8 Size: 457 889 1629 43 Fragments arranged by size: 1629 1425 942 889 457 275 54 50 43 18 18 6 EagI C'GGCC_G Cuts at: 1537 1812 2790 3253 1537 Size: 275 978 463 4090 Fragments arranged by size: 4090 978 463 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2143 2832 3736 1353 Size: 790 689 904 3423 Fragments arranged by size: 3423 904 790 689 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3013 4393 4767 4913 387 Size: 1064 138 1424 1380 374 146 1280 Fragments arranged by size: 1424 1380 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2442 2561 3869 4466 5253 5521 1239 Size: 393 810 119 1308 597 787 268 1524 Fragments arranged by size: 1524 1308 810 787 597 393 268 119 EcoO109I rG'GnC_Cy Cuts at: 2360 2360 Size: 5806 EcoRI G'AATT_C Cuts at: 3115 3493 3115 Size: 378 5428 FspI TGC'GCA Cuts at: 2745 4312 2745 Size: 1567 4239 HaeII r_GCGC'y Cuts at: 1678 3018 4953 1678 Size: 1340 1935 2531 Fragments arranged by size: 2531 1935 1340 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4447 4479 4521 4553 1373 Size: 32 135 32 2875 32 42 32 2626 Fragments arranged by size: 2875 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3150 3396 669 Size: 988 281 1212 246 3079 Fragments arranged by size: 3079 1212 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5806 HpaI GTT'AAC Cuts at: 3396 3396 Size: 5806 KpnI G_GTAC'C Cuts at: 2874 3131 2874 Size: 257 5549 MfeI C'AATT_G Cuts at: 3405 3405 Size: 5806 MluI A'CGCG_T Cuts at: 5682 5682 Size: 5806 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2243 2981 4920 5104 1305 Size: 938 738 1939 184 2007 Fragments arranged by size: 2007 1939 938 738 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3984 Fragments arranged by size: 3984 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2804 3764 4123 4282 518 Size: 648 1638 960 359 159 2042 Fragments arranged by size: 2042 1638 960 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2789 2795 3222 3899 5047 5292 1606 Size: 1183 6 427 677 1148 245 2120 Fragments arranged by size: 2120 1183 1148 677 427 245 6 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4315 Fragments arranged by size: 4315 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5806 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5806 NotI GC'GGCC_GC Cuts at: 2790 3253 2790 Size: 463 5343 NspI r_CATG'y Cuts at: 1132 1229 2085 3164 4709 1132 Size: 97 856 1079 1545 2229 Fragments arranged by size: 2229 1545 1079 856 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5806 PciI A'CATG_T Cuts at: 4705 4705 Size: 5806 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5806 PfoI T'CCnGG_A Cuts at: 2320 2473 2320 Size: 153 5653 PmeI GTTT'AAAC Cuts at: 3110 3110 Size: 5806 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3875 3907 5388 5420 3875 Size: 32 1481 32 4261 Fragments arranged by size: 4261 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2360 2360 Size: 5806 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5806 PsiI TTA'TAA Cuts at: 3376 3376 Size: 5806 PstI C_TGCA'G Cuts at: 830 2063 2284 3040 3158 830 Size: 1233 221 756 118 3478 Fragments arranged by size: 3478 1233 756 221 118 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5806 SacII CC_GC'GG Cuts at: 2790 2796 2790 Size: 6 5800 SalI G'TCGA_C Cuts at: 1936 3148 1936 Size: 1212 4594 SapI GCTCTTCn'nnn_ Cuts at: 2832 2832 Size: 5806 SbfI CC_TGCA'GG Cuts at: 3158 3158 Size: 5806 ScaI AGT'ACT Cuts at: 4054 5766 4054 Size: 1712 4094 SfcI C'TryA_G Cuts at: 826 1045 2059 2280 2355 3036 3154 4289 4970 Size: 219 1014 221 75 681 118 1135 681 Cuts at: 4970 5161 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1014 681 681 221 219 191 118 75 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5806 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5806 SmaI CCC'GGG Cuts at: 1872 2499 2579 3133 1872 Size: 627 80 554 4545 Fragments arranged by size: 4545 627 554 80 SmlI C'TyrA_G Cuts at: 820 1017 1943 3910 4811 5073 820 Size: 197 926 1967 901 262 1553 Fragments arranged by size: 1967 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3597 493 Size: 3104 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5806 SphI G_CATG'C Cuts at: 1132 3164 1132 Size: 2032 3774 SspI AAT'ATT Cuts at: 5 52 2702 3730 5 Size: 47 2650 1028 2081 Fragments arranged by size: 2650 2081 1028 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5806 StyI C'CwwG_G Cuts at: 513 1065 2004 3245 513 Size: 552 939 1241 3074 Fragments arranged by size: 3074 1241 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2603 3833 3850 4003 4188 4633 325 Size: 1255 1023 1230 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1230 1023 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4052 5764 96 Size: 274 80 33 51 1443 2075 1712 138 Fragments arranged by size: 2075 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5766 5798 5766 Size: 32 5774 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5806 XbaI T'CTAG_A Cuts at: 3142 3142 Size: 5806 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5806 XmaI C'CCGG_G Cuts at: 1870 2497 2577 3131 1870 Size: 627 80 554 4545 Fragments arranged by size: 4545 627 554 80 XmnI GAAnn'nnTTC Cuts at: 3935 3935 Size: 5806 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3614 276 Size: 53 83 186 936 2080 2468 Fragments arranged by size: 2468 2080 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI XcmI