(Linear) (Six-base) MAPSORT of: KIEE5555.seq Check: 9434 from: 1 to: 1101 KIEE5555.orf With 182 enzymes: SgfI * January 29, 2014 12:05 .. Acc65I G'GTAC_C Cuts at: 0 865 1101 Size: 865 236 AclI AA'CG_TT Cuts at: 0 11 1101 Size: 11 1090 AfeI AGC'GCT Cuts at: 0 1011 1101 Size: 1011 90 AgeI A'CCGG_T Cuts at: 0 480 1101 Size: 480 621 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 290 1101 Size: 290 811 ApoI r'AATT_y Cuts at: 0 103 440 1101 Size: 103 337 661 Fragments arranged by size: 661 337 103 AseI AT'TA_AT Cuts at: 0 242 1101 Size: 242 859 AvaI C'yCGr_G Cuts at: 0 492 572 1101 Size: 492 80 529 Fragments arranged by size: 529 492 80 BanI G'GyrC_C Cuts at: 0 865 1101 Size: 865 236 BanII G_rGCy'C Cuts at: 0 499 1101 Size: 499 602 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 603 637 1101 Size: 603 34 464 Fragments arranged by size: 603 464 34 BglI GCCn_nnn'nGGC Cuts at: 0 775 1101 Size: 775 326 Bme1580I G_kGCm'C Cuts at: 0 341 573 1101 Size: 341 232 528 Fragments arranged by size: 528 341 232 BmrI ACTGGGnnnn_n' Cuts at: 0 13 1101 Size: 13 1088 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 437 556 1101 Size: 437 119 545 Fragments arranged by size: 545 437 119 BsaI GGTCTCn'nnnn_ Cuts at: 0 574 1101 Size: 574 527 BsaWI w'CCGG_w Cuts at: 0 480 1101 Size: 480 621 BseYI C'CCAG_C Cuts at: 0 293 886 1101 Size: 293 593 215 Fragments arranged by size: 593 293 215 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 695 1101 Size: 695 406 BsiEI CG_ry'CG Cuts at: 0 788 1101 Size: 788 313 BsiHKAI G_wGCw'C Cuts at: 0 822 1101 Size: 822 279 BsmI GAATG_Cn' Cuts at: 0 103 1101 Size: 103 998 Bsp1286I G_dGCh'C Cuts at: 0 341 499 573 822 1101 Size: 341 158 74 249 279 Fragments arranged by size: 341 279 249 158 74 BsrDI GCAATG_nn' Cuts at: 0 290 867 1101 Size: 290 577 234 Fragments arranged by size: 577 290 234 BsrFI r'CCGG_y Cuts at: 0 480 1101 Size: 480 621 BstAPI GCAn_nnn'nTGC Cuts at: 0 289 1101 Size: 289 812 BstXI CCAn_nnnn'nTGG Cuts at: 0 801 1101 Size: 801 300 BtgI C'CryG_G Cuts at: 0 782 788 1101 Size: 782 6 313 Fragments arranged by size: 782 313 6 EaeI y'GGCC_r Cuts at: 0 767 785 791 1101 Size: 767 18 6 310 Fragments arranged by size: 767 310 18 6 EagI C'GGCC_G Cuts at: 0 785 1101 Size: 785 316 EarI CTCTTCn'nnn_ Cuts at: 0 138 827 1101 Size: 138 689 274 Fragments arranged by size: 689 274 138 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1008 1101 Size: 1008 93 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 437 556 1101 Size: 437 119 545 Fragments arranged by size: 545 437 119 EcoO109I rG'GnC_Cy Cuts at: 0 355 1101 Size: 355 746 FspI TGC'GCA Cuts at: 0 740 1101 Size: 740 361 HaeII r_GCGC'y Cuts at: 0 1013 1101 Size: 1013 88 KpnI G_GTAC'C Cuts at: 0 869 1101 Size: 869 232 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 238 976 1101 Size: 238 738 125 Fragments arranged by size: 738 238 125 MslI CAynn'nnrTG Cuts at: 0 799 1101 Size: 799 302 MspA1I CmG'CkG Cuts at: 0 784 790 1101 Size: 784 6 311 Fragments arranged by size: 784 311 6 NotI GC'GGCC_GC Cuts at: 0 785 1101 Size: 785 316 NspI r_CATG'y Cuts at: 0 80 1101 Size: 80 1021 PfoI T'CCnGG_A Cuts at: 0 315 468 1101 Size: 315 153 633 Fragments arranged by size: 633 315 153 PpuMI rG'GwC_Cy Cuts at: 0 355 1101 Size: 355 746 PstI C_TGCA'G Cuts at: 0 58 279 1035 1101 Size: 58 221 756 66 Fragments arranged by size: 756 221 66 58 SacII CC_GC'GG Cuts at: 0 785 791 1101 Size: 785 6 310 Fragments arranged by size: 785 310 6 SapI GCTCTTCn'nnn_ Cuts at: 0 827 1101 Size: 827 274 SfcI C'TryA_G Cuts at: 0 54 275 350 1031 1101 Size: 54 221 75 681 70 Fragments arranged by size: 681 221 75 70 54 SmaI CCC'GGG Cuts at: 0 494 574 1101 Size: 494 80 527 Fragments arranged by size: 527 494 80 SspI AAT'ATT Cuts at: 0 697 1101 Size: 697 404 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 598 1101 Size: 598 503 XmaI C'CCGG_G Cuts at: 0 492 572 1101 Size: 492 80 529 Fragments arranged by size: 529 492 80 Enzymes that do cut: Acc65I AclI AfeI AgeI AlfI ApoI AseI AvaI BanI BanII BcgI BglI Bme1580I BmrI BpmI BsaI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BsrDI BsrFI BstAPI BstXI BtgI EaeI EagI EarI EciI Eco57MI EcoO109I FspI HaeII KpnI MmeI MslI MspA1I NotI NspI PfoI PpuMI PstI SacII SapI SfcI SmaI SspI TaqII XmaI Enzymes that do not cut: AarI AatII AccI AcuI AflII AflIII AhdI AleI AloI AlwNI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI BseRI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI Hin4I HincII HindIII HpaI KasI MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI StuI StyI SwaI TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI