(Circular) (Six-base) MAPSORT of: pFN21AE2336.seq Check: 7140 from: 1 to: 8680 pFN21AE2336 8680 bp With 182 enzymes: SgfI * July 4, 2020 18:17 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6490 278 Size: 53 83 186 936 4954 2468 Fragments arranged by size: 4954 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5877 6023 1937 Size: 3940 146 4594 Fragments arranged by size: 4594 3940 146 Acc65I G'GTAC_C Cuts at: 6001 6001 Size: 8680 AclI AA'CG_TT Cuts at: 1563 6807 7180 1563 Size: 5244 373 3063 Fragments arranged by size: 5244 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4101 4102 5028 5198 6743 8127 8395 1632 Size: 2469 1 926 170 1545 1384 268 1917 Fragments arranged by size: 2469 1917 1545 1384 926 268 170 1 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8483 AflIII A'CryG_T Cuts at: 1203 2065 7579 8556 1203 Size: 862 5514 977 1327 Fragments arranged by size: 5514 1327 977 862 AgeI A'CCGG_T Cuts at: 7511 7511 Size: 8680 AhdI GACnn_n'nnGTC Cuts at: 7409 7409 Size: 8680 AleI CACnn'nnGTG Cuts at: 1166 3020 1166 Size: 1854 6826 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2688 1184 Size: 1504 7176 AlwNI CAG_nnn'CTG Cuts at: 2681 3814 4780 6423 7995 2681 Size: 1133 966 1643 1572 3366 Fragments arranged by size: 3366 1643 1572 1133 966 ApaLI G'TGCA_C Cuts at: 6737 7893 6737 Size: 1156 7524 ApoI r'AATT_y Cuts at: 1493 2292 2962 3744 4302 4464 5295 5669 5989 Size: 799 670 782 558 162 831 374 320 Cuts at: 5989 6215 6367 8476 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 831 799 782 670 558 374 320 226 162 152 AseI AT'TA_AT Cuts at: 160 1032 2355 7234 160 Size: 872 1323 4879 1606 Fragments arranged by size: 4879 1606 1323 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8680 AvaI C'yCGr_G Cuts at: 1870 1943 3380 3460 6005 1870 Size: 73 1437 80 2545 4545 Fragments arranged by size: 4545 2545 1437 80 73 BamHI G'GATC_C Cuts at: 5410 5525 5626 6010 6377 5410 Size: 115 101 384 367 7713 Fragments arranged by size: 7713 384 367 115 101 BanI G'GyrC_C Cuts at: 618 943 1160 1787 6001 7456 618 Size: 325 217 627 4214 1455 1842 Fragments arranged by size: 4214 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4737 5999 1391 Size: 3346 1262 4072 Fragments arranged by size: 4072 3346 1262 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2160 2573 3966 4135 5761 928 Size: 971 261 413 1393 169 1626 3847 Fragments arranged by size: 3847 1626 1393 971 413 261 169 BbvCI CC'TCA_GC Cuts at: 3115 3115 Size: 8680 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2977 3011 6871 6905 1903 Size: 34 50 34 956 34 3860 34 3678 Fragments arranged by size: 3860 3678 956 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5497 6572 7782 5497 Size: 1075 1210 6395 Fragments arranged by size: 6395 1210 1075 BclI T'GATC_A Cuts at: 1548 2620 3039 1548 Size: 1072 419 7189 Fragments arranged by size: 7189 1072 419 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7291 136 Size: 107 122 71 631 605 5619 1525 Fragments arranged by size: 5619 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4282 4349 5310 5485 8675 4282 Size: 67 961 175 3190 4287 Fragments arranged by size: 4287 3190 961 175 67 BlpI GC'TnA_GC Cuts at: 6097 6097 Size: 8680 Bme1580I G_kGCm'C Cuts at: 1790 6741 7897 1790 Size: 4951 1156 2573 Fragments arranged by size: 4951 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8680 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4046 4225 5840 Size: 416 53 83 399 24 2629 179 1615 Cuts at: 5840 6411 7369 442 Size: 571 958 1753 Fragments arranged by size: 2629 1753 1615 958 571 416 399 179 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8680 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3912 4046 4712 7340 1239 Size: 2673 134 666 2628 2579 Fragments arranged by size: 2673 2628 2579 666 134 Bpu10I CC'TnA_GC Cuts at: 3115 4176 3115 Size: 1061 7619 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6805 7670 7968 6805 Size: 865 298 7517 Fragments arranged by size: 7517 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3269 3990 4570 7343 882 Size: 617 1770 721 580 2773 2219 Fragments arranged by size: 2773 2219 1770 721 617 580 BsaAI yAC'GTr Cuts at: 493 1204 3022 6471 493 Size: 711 1818 3449 2702 Fragments arranged by size: 3449 2702 1818 711 BsaBI GATnn'nnATC Cuts at: 3573 4449 8674 3573 Size: 876 4225 3579 Fragments arranged by size: 4225 3579 876 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6487 6869 7504 275 Size: 53 83 186 936 4954 382 635 1451 Fragments arranged by size: 4954 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4429 5234 7113 7511 7785 7932 8343 4429 Size: 805 1879 398 274 147 411 4766 Fragments arranged by size: 4766 1879 805 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4251 4281 1178 Size: 30 165 30 2848 30 5577 Fragments arranged by size: 5577 2848 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2192 2803 3017 3487 4082 4272 1186 Size: 1006 611 214 470 595 190 5594 Fragments arranged by size: 5594 1006 611 595 470 214 190 BseYI C'CCAG_C Cuts at: 2489 2720 7883 8379 2489 Size: 231 5163 496 2790 Fragments arranged by size: 5163 2790 496 231 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3260 3714 3860 5665 1163 Size: 573 1524 454 146 1805 4178 Fragments arranged by size: 4178 1805 1524 573 454 146 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6130 6891 7919 1540 Size: 275 186 4129 761 1028 2301 Fragments arranged by size: 4129 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3027 3381 3541 4024 4132 5427 6741 6826 7897 Size: 354 160 483 108 1295 1314 85 1071 Cuts at: 7897 3027 Size: 3810 Fragments arranged by size: 3810 1314 1295 1071 483 354 160 108 85 BsiWI C'GTAC_G Cuts at: 2406 2406 Size: 8680 BsmI GAATG_Cn' Cuts at: 5452 6191 6284 5452 Size: 739 93 7848 Fragments arranged by size: 7848 739 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3027 3381 3541 4024 4132 4737 5427 Size: 399 1237 354 160 483 108 605 690 Cuts at: 5427 5999 6741 6826 7897 1391 Size: 572 742 85 1071 2174 Fragments arranged by size: 2174 1237 1071 742 690 605 572 483 399 354 160 108 85 BspEI T'CCGG_A Cuts at: 8343 8343 Size: 8680 BspHI T'CATG_A Cuts at: 6569 8299 6569 Size: 1730 6950 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4927 6035 844 Size: 4083 1108 3489 Fragments arranged by size: 4083 3489 1108 BsrBI CCG'CTC Cuts at: 1394 1823 2160 6567 1394 Size: 429 337 4407 3507 Fragments arranged by size: 4407 3507 429 337 BsrDI GCAATG_nn' Cuts at: 65 1248 4541 5694 7175 7349 65 Size: 1183 3293 1153 1481 174 1396 Fragments arranged by size: 3293 1481 1396 1183 1153 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7324 7511 1700 Size: 110 5514 187 2869 Fragments arranged by size: 5514 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 5151 96 Size: 5055 3625 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8680 BssSI C'ACGA_G Cuts at: 6740 7752 6740 Size: 1012 7668 BstAPI GCAn_nnn'nTGC Cuts at: 794 2681 3701 794 Size: 1887 1020 5773 Fragments arranged by size: 5773 1887 1020 BstBI TT'CG_AA Cuts at: 3726 7484 3726 Size: 3758 4922 BstEII G'GTnAC_C Cuts at: 1184 3097 1184 Size: 1913 6767 BstXI CCAn_nnnn'nTGG Cuts at: 2341 5106 2341 Size: 2765 5915 BstYI r'GATC_y Cuts at: 1972 4282 4349 5310 5410 5485 5525 5626 6010 Size: 2310 67 961 100 75 40 101 384 Cuts at: 6010 6377 6762 6779 8231 8317 8329 8675 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 2310 1977 1452 961 385 384 367 346 101 100 86 75 67 40 17 12 BstZ17I GTA'TAC Cuts at: 5878 5878 Size: 8680 Bsu36I CC'TnA_GG Cuts at: 2951 5367 2951 Size: 2416 6264 BtgI C'CryG_G Cuts at: 513 1065 2379 2418 2559 3342 513 Size: 552 1314 39 141 783 5851 Fragments arranged by size: 5851 1314 783 552 141 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7941 Fragments arranged by size: 7941 683 56 BtsI GCAGTG_nn' Cuts at: 2660 2682 3040 3072 3654 4167 5677 6196 6990 Size: 22 358 32 582 513 1510 519 794 Cuts at: 6990 7010 2660 Size: 20 4330 Fragments arranged by size: 4330 1510 794 582 519 513 358 32 22 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8680 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4184 4219 529 Size: 35 3620 35 4990 Fragments arranged by size: 4990 3620 35 35 DraI TTT'AAA Cuts at: 2098 3897 4470 5984 2098 Size: 1799 573 1514 4794 Fragments arranged by size: 4794 1799 1514 573 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7687 809 Size: 507 6371 1802 Fragments arranged by size: 6371 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2104 2556 4578 Size: 54 1425 50 275 18 274 452 2022 Cuts at: 4578 6127 7016 8645 8 Size: 1549 889 1629 43 Fragments arranged by size: 2022 1629 1549 1425 889 452 275 274 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6127 1537 Size: 275 4315 4090 Fragments arranged by size: 4315 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3110 4387 4758 6610 1353 Size: 1757 1277 371 1852 3423 Fragments arranged by size: 3423 1852 1757 1277 371 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7267 7641 7787 387 Size: 1064 138 5678 374 146 1280 Fragments arranged by size: 5678 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3912 4046 4101 4102 4712 5028 5198 Size: 393 2280 134 55 1 610 316 170 Cuts at: 5198 6743 7340 8127 8395 1239 Size: 1545 597 787 268 1524 Fragments arranged by size: 2280 1545 1524 787 610 597 393 316 268 170 134 55 1 EcoNI CCTnn'n_nnAGG Cuts at: 2174 2950 2174 Size: 776 7904 EcoO109I rG'GnC_Cy Cuts at: 4902 4902 Size: 8680 EcoRI G'AATT_C Cuts at: 5989 6367 5989 Size: 378 8302 EcoRV GAT'ATC Cuts at: 3189 3189 Size: 8680 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4132 4132 Size: 8680 FspI TGC'GCA Cuts at: 7186 7186 Size: 8680 HaeII r_GCGC'y Cuts at: 1678 4678 7827 1678 Size: 3000 3149 2531 Fragments arranged by size: 3149 3000 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2575 2607 3116 3148 3173 Size: 32 135 32 1003 32 509 32 25 Cuts at: 3173 3205 3254 3268 3286 3300 3577 3609 3872 Size: 32 49 14 18 14 277 32 263 Cuts at: 3872 3904 5112 5144 7321 7353 7395 7427 1373 Size: 32 1208 32 2177 32 42 32 2626 Fragments arranged by size: 2626 2177 1208 1003 509 277 263 135 49 42 32 32 32 32 32 32 32 32 32 32 25 18 14 14 HincII GTy'rAC Cuts at: 669 1657 1938 4378 6024 6270 669 Size: 988 281 2440 1646 246 3079 Fragments arranged by size: 3079 2440 1646 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8680 HpaI GTT'AAC Cuts at: 4378 6270 4378 Size: 1892 6788 KpnI G_GTAC'C Cuts at: 6005 6005 Size: 8680 MfeI C'AATT_G Cuts at: 6279 6279 Size: 8680 MluI A'CGCG_T Cuts at: 2065 8556 2065 Size: 6491 2189 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3543 5521 7794 7978 1305 Size: 2238 1978 2273 184 2007 Fragments arranged by size: 2273 2238 2007 1978 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2106 2558 10 Size: 54 1425 343 274 452 6132 Fragments arranged by size: 6132 1425 452 343 274 54 MslI CAynn'nnrTG Cuts at: 518 1166 2558 3020 4500 4970 4976 5430 6638 Size: 648 1392 462 1480 470 6 454 1208 Cuts at: 6638 6997 7156 518 Size: 359 159 2042 Fragments arranged by size: 2042 1480 1392 1208 648 470 462 454 359 159 6 MspA1I CmG'CkG Cuts at: 1606 2041 3208 3559 4516 4842 5849 6096 6773 Size: 435 1167 351 957 326 1007 247 677 Cuts at: 6773 7921 8166 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1167 1148 1007 957 677 435 351 326 247 245 NcoI C'CATG_G Cuts at: 513 1065 2379 2559 513 Size: 552 1314 180 6634 Fragments arranged by size: 6634 1314 552 180 NdeI CA'TA_TG Cuts at: 387 2520 387 Size: 2133 6547 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8680 NotI GC'GGCC_GC Cuts at: 6127 6127 Size: 8680 NspI r_CATG'y Cuts at: 1132 1229 3239 6038 7583 1132 Size: 97 2010 2799 1545 2229 Fragments arranged by size: 2799 2229 2010 1545 97 PasI CC'CwG_GG Cuts at: 1348 3450 1348 Size: 2102 6578 PciI A'CATG_T Cuts at: 7579 7579 Size: 8680 PflMI CCAn_nnn'nTGG Cuts at: 1108 5903 1108 Size: 4795 3885 PfoI T'CCnGG_A Cuts at: 3675 3934 5661 3675 Size: 259 1727 6694 Fragments arranged by size: 6694 1727 259 PmeI GTTT'AAAC Cuts at: 5984 5984 Size: 8680 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2045 2077 4802 4834 6749 6781 8262 8294 2045 Size: 32 2725 32 1915 32 1481 32 2431 Fragments arranged by size: 2725 2431 1915 1481 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4902 4902 Size: 8680 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8680 PsiI TTA'TAA Cuts at: 2843 6250 2843 Size: 3407 5273 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2390 2422 4314 4346 5135 5167 2390 Size: 32 1892 32 789 32 5903 Fragments arranged by size: 5903 1892 789 32 32 32 PstI C_TGCA'G Cuts at: 830 2046 2667 4174 6032 830 Size: 1216 621 1507 1858 3478 Fragments arranged by size: 3478 1858 1507 1216 621 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8680 PvuII CAG'CTG Cuts at: 3208 3559 3208 Size: 351 8329 SalI G'TCGA_C Cuts at: 1936 6022 1936 Size: 4086 4594 SanDI GG'GwC_CC Cuts at: 4902 4902 Size: 8680 SbfI CC_TGCA'GG Cuts at: 6032 6032 Size: 8680 ScaI AGT'ACT Cuts at: 6928 8640 6928 Size: 1712 6968 SfcI C'TryA_G Cuts at: 826 1045 2042 2663 4170 4320 6028 7163 7844 Size: 219 997 621 1507 150 1708 1135 681 Cuts at: 7844 8035 826 Size: 191 1471 Fragments arranged by size: 1708 1507 1471 1135 997 681 621 219 191 150 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8680 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8680 SmaI CCC'GGG Cuts at: 1872 6007 1872 Size: 4135 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 6784 7685 7947 820 Size: 197 926 4841 901 262 1553 Fragments arranged by size: 4841 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6471 493 Size: 5978 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8680 SphI G_CATG'C Cuts at: 1132 6038 1132 Size: 4906 3774 SspI AAT'ATT Cuts at: 5 52 3481 6604 5 Size: 47 3429 3123 2081 Fragments arranged by size: 3429 3123 2081 47 StuI AGG'CCT Cuts at: 1517 3294 4103 1517 Size: 1777 809 6094 Fragments arranged by size: 6094 1777 809 StyI C'CwwG_G Cuts at: 513 1065 2170 2379 2559 4589 4605 4615 5075 Size: 552 1105 209 180 2030 16 10 460 Cuts at: 5075 6119 513 Size: 1044 3074 Fragments arranged by size: 3074 2030 1105 1044 552 460 209 180 16 10 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3442 6707 6724 6877 7062 7507 325 Size: 1255 1862 3265 17 153 185 445 1498 Fragments arranged by size: 3265 1862 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5151 6926 8638 Size: 274 80 33 51 1443 3174 1775 1712 Cuts at: 8638 96 Size: 138 Fragments arranged by size: 3174 1775 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8640 8672 8640 Size: 32 8648 Tth111I GACn'n_nGTC Cuts at: 1316 4834 1316 Size: 3518 5162 XbaI T'CTAG_A Cuts at: 3998 6016 3998 Size: 2018 6662 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2386 5103 2386 Size: 2717 5963 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8680 XmaI C'CCGG_G Cuts at: 1870 6005 1870 Size: 4135 4545 XmnI GAAnn'nnTTC Cuts at: 2774 4115 4306 5248 5487 6809 2774 Size: 1341 191 942 239 1322 4645 Fragments arranged by size: 4645 1341 1322 942 239 191 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6488 276 Size: 53 83 186 936 4954 2468 Fragments arranged by size: 4954 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsmBI DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI