(Circular) (Six-base) MAPSORT of: pFN21AE6770.seq Check: 7458 from: 1 to: 6370 pFN21AE6770 6370 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2989 3644 2989 Size: 655 5715 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4180 278 Size: 53 83 186 936 2644 2468 Fragments arranged by size: 2644 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3713 1937 Size: 1776 4594 Acc65I G'GTAC_C Cuts at: 3691 3691 Size: 6370 AclI AA'CG_TT Cuts at: 1563 4497 4870 1563 Size: 2934 373 3063 Fragments arranged by size: 3063 2934 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2533 2645 2985 4433 5817 6085 1632 Size: 901 112 340 1448 1384 268 1917 Fragments arranged by size: 1917 1448 1384 901 340 268 112 AflII C'TTAA_G Cuts at: 820 1017 2173 820 Size: 197 1156 5017 Fragments arranged by size: 5017 1156 197 AflIII A'CryG_T Cuts at: 1203 3089 3516 5269 6246 1203 Size: 1886 427 1753 977 1327 Fragments arranged by size: 1886 1753 1327 977 427 AgeI A'CCGG_T Cuts at: 5201 5201 Size: 6370 AhdI GACnn_n'nnGTC Cuts at: 5099 5099 Size: 6370 AleI CACnn'nnGTG Cuts at: 1166 2297 2550 1166 Size: 1131 253 4986 Fragments arranged by size: 4986 1131 253 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3021 1184 Size: 1837 4533 AlwNI CAG_nnn'CTG Cuts at: 2677 3074 3661 4113 5685 2677 Size: 397 587 452 1572 3362 Fragments arranged by size: 3362 1572 587 452 397 ApaLI G'TGCA_C Cuts at: 4427 5583 4427 Size: 1156 5214 ApoI r'AATT_y Cuts at: 1493 2249 2315 2324 2407 3079 3679 3905 4057 Size: 756 66 9 83 672 600 226 152 Cuts at: 4057 6166 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 756 672 600 226 152 83 66 9 AseI AT'TA_AT Cuts at: 160 1032 4924 160 Size: 872 3892 1606 Fragments arranged by size: 3892 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6370 AvaI C'yCGr_G Cuts at: 1870 1943 3344 3695 1870 Size: 73 1401 351 4545 Fragments arranged by size: 4545 1401 351 73 BamHI G'GATC_C Cuts at: 3565 3700 4067 3565 Size: 135 367 5868 Fragments arranged by size: 5868 367 135 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2117 3691 5146 618 Size: 325 217 627 330 1574 1455 1842 Fragments arranged by size: 1842 1574 1455 627 330 325 217 BanII G_rGCy'C Cuts at: 1391 2340 2503 3324 3689 1391 Size: 949 163 821 365 4072 Fragments arranged by size: 4072 949 821 365 163 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2226 2410 2562 928 Size: 971 327 184 152 4736 Fragments arranged by size: 4736 971 327 184 152 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4561 4595 1903 Size: 34 50 34 2540 34 3678 Fragments arranged by size: 3678 2540 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2747 4262 5472 2747 Size: 1515 1210 3645 Fragments arranged by size: 3645 1515 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6370 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4981 136 Size: 107 122 71 631 605 3309 1525 Fragments arranged by size: 3309 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3056 6365 3056 Size: 3309 3061 BlpI GC'TnA_GC Cuts at: 3067 3787 3067 Size: 720 5650 Bme1580I G_kGCm'C Cuts at: 1790 2616 2746 3251 3282 4431 5587 1790 Size: 826 130 505 31 1149 1156 2573 Fragments arranged by size: 2573 1156 1149 826 505 130 31 BmgBI CAC'GTC Cuts at: 1318 2611 3252 3517 1318 Size: 1293 641 265 4171 Fragments arranged by size: 4171 1293 641 265 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2466 4101 5059 Size: 416 53 83 399 24 1049 1635 958 Cuts at: 5059 442 Size: 1753 Fragments arranged by size: 1753 1635 1049 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6370 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3604 3604 Size: 6370 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2363 2495 2930 3030 5030 1239 Size: 1124 132 435 100 2000 2579 Fragments arranged by size: 2579 2000 1124 435 132 100 Bpu10I CC'TnA_GC Cuts at: 3487 3487 Size: 6370 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2579 4495 5360 5658 2579 Size: 1916 865 298 3291 Fragments arranged by size: 3291 1916 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3633 5033 882 Size: 617 2134 1400 2219 Fragments arranged by size: 2219 2134 1400 617 BsaAI yAC'GTr Cuts at: 493 1204 4161 493 Size: 711 2957 2702 Fragments arranged by size: 2957 2702 711 BsaBI GATnn'nnATC Cuts at: 6364 6364 Size: 6370 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4177 4559 5194 275 Size: 53 83 186 936 2644 382 635 1451 Fragments arranged by size: 2644 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3573 4803 5201 5475 5622 6033 3573 Size: 1230 398 274 147 411 3910 Fragments arranged by size: 3910 1230 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3484 3514 3521 3551 1178 Size: 30 165 30 2081 30 7 30 3997 Fragments arranged by size: 3997 2081 165 30 30 30 30 7 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3275 1186 Size: 2089 4281 BseYI C'CCAG_C Cuts at: 3600 5573 6069 3600 Size: 1973 496 3901 Fragments arranged by size: 3901 1973 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2188 3685 1163 Size: 573 452 1497 3848 Fragments arranged by size: 3848 1497 573 452 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3574 3820 4581 5609 1540 Size: 275 186 1573 246 761 1028 2301 Fragments arranged by size: 2301 1573 1028 761 275 246 186 BsiHKAI G_wGCw'C Cuts at: 2340 4431 4516 5587 2340 Size: 2091 85 1071 3123 Fragments arranged by size: 3123 2091 1071 85 BsiWI C'GTAC_G Cuts at: 2112 2112 Size: 6370 BsmI GAATG_Cn' Cuts at: 2353 3272 3881 3974 2353 Size: 919 609 93 4749 Fragments arranged by size: 4749 919 609 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2601 2601 Size: 6370 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2340 2503 2616 2746 3251 3282 3324 Size: 399 550 163 113 130 505 31 42 Cuts at: 3324 3689 4431 4516 5587 1391 Size: 365 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 550 505 399 365 163 130 113 85 42 31 BspEI T'CCGG_A Cuts at: 6033 6033 Size: 6370 BspHI T'CATG_A Cuts at: 2748 2823 3309 4259 5989 2748 Size: 75 486 950 1730 3129 Fragments arranged by size: 3129 1730 950 486 75 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2989 3049 3644 3725 844 Size: 2145 60 595 81 3489 Fragments arranged by size: 3489 2145 595 81 60 BsrBI CCG'CTC Cuts at: 1394 1823 3066 4257 1394 Size: 429 1243 1191 3507 Fragments arranged by size: 3507 1243 1191 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2649 2892 3451 4865 5039 65 Size: 1183 1401 243 559 1414 174 1396 Fragments arranged by size: 1414 1401 1396 1183 559 243 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3150 5014 5201 1700 Size: 110 1340 1864 187 2869 Fragments arranged by size: 2869 1864 1340 187 110 BsrGI T'GTAC_A Cuts at: 96 2397 96 Size: 2301 4069 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6370 BssSI C'ACGA_G Cuts at: 4430 5442 4430 Size: 1012 5358 BstAPI GCAn_nnn'nTGC Cuts at: 794 3504 794 Size: 2710 3660 BstBI TT'CG_AA Cuts at: 5174 5174 Size: 6370 BstEII G'GTnAC_C Cuts at: 1184 2635 1184 Size: 1451 4919 BstXI CCAn_nnnn'nTGG Cuts at: 2215 2215 Size: 6370 BstYI r'GATC_y Cuts at: 1972 3056 3565 3700 4067 4452 4469 5921 6007 Size: 1084 509 135 367 385 17 1452 86 Cuts at: 6007 6019 6365 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1084 509 385 367 346 135 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2187 3111 2187 Size: 924 5446 BtgI C'CryG_G Cuts at: 513 1065 3525 513 Size: 552 2460 3358 Fragments arranged by size: 3358 2460 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5631 Fragments arranged by size: 5631 683 56 BtsI GCAGTG_nn' Cuts at: 3505 3567 3650 3886 4680 4700 3505 Size: 62 83 236 794 20 5175 Fragments arranged by size: 5175 794 236 83 62 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6370 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3514 3549 529 Size: 35 2950 35 3350 Fragments arranged by size: 3350 2950 35 35 DraI TTT'AAA Cuts at: 3674 3674 Size: 6370 DraIII CAC_nnn'GTG Cuts at: 2865 2865 Size: 6370 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3155 5377 809 Size: 507 1839 2222 1802 Fragments arranged by size: 2222 1839 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3607 3817 4706 Size: 54 1425 50 275 18 1777 210 889 Cuts at: 4706 6335 8 Size: 1629 43 Fragments arranged by size: 1777 1629 1425 889 275 210 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3817 1537 Size: 275 2005 4090 Fragments arranged by size: 4090 2005 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2262 2435 3595 4300 1353 Size: 909 173 1160 705 3423 Fragments arranged by size: 3423 1160 909 705 173 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2087 2093 2153 4957 5331 5477 Size: 1064 138 498 6 60 2804 374 146 Cuts at: 5477 387 Size: 1280 Fragments arranged by size: 2804 1280 1064 498 374 146 138 60 6 EcoICRI GAG'CTC Cuts at: 2338 2338 Size: 6370 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2363 2495 2533 2645 2930 2985 3030 Size: 393 731 132 38 112 285 55 45 Cuts at: 3030 4433 5030 5817 6085 1239 Size: 1403 597 787 268 1524 Fragments arranged by size: 1524 1403 787 731 597 393 285 268 132 112 55 45 38 EcoO109I rG'GnC_Cy Cuts at: 2425 2661 2796 3099 3553 2425 Size: 236 135 303 454 5242 Fragments arranged by size: 5242 454 303 236 135 EcoRI G'AATT_C Cuts at: 2407 3679 4057 2407 Size: 1272 378 4720 Fragments arranged by size: 4720 1272 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2851 2851 Size: 6370 FspI TGC'GCA Cuts at: 2288 4876 2288 Size: 2588 3782 FspAI rTGC'GCAy Cuts at: 2288 2288 Size: 6370 HaeII r_GCGC'y Cuts at: 1678 5517 1678 Size: 3839 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2521 2553 3325 3357 3385 Size: 32 135 32 949 32 772 32 28 Cuts at: 3385 3417 5011 5043 5085 5117 1373 Size: 32 1594 32 42 32 2626 Fragments arranged by size: 2626 1594 949 772 135 42 32 32 32 32 32 32 32 28 HincII GTy'rAC Cuts at: 669 1657 1938 3714 3960 669 Size: 988 281 1776 246 3079 Fragments arranged by size: 3079 1776 988 281 246 HindIII A'AGCT_T Cuts at: 748 3142 748 Size: 2394 3976 HpaI GTT'AAC Cuts at: 3960 3960 Size: 6370 KpnI G_GTAC'C Cuts at: 3695 3695 Size: 6370 MfeI C'AATT_G Cuts at: 3969 3969 Size: 6370 MluI A'CGCG_T Cuts at: 6246 6246 Size: 6370 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3279 5484 5668 1305 Size: 1974 2205 184 2007 Fragments arranged by size: 2205 2007 1974 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3609 10 Size: 54 1425 343 1777 2771 Fragments arranged by size: 2771 1777 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2213 2297 2550 2753 2999 4328 4687 Size: 648 1047 84 253 203 246 1329 359 Cuts at: 4687 4846 518 Size: 159 2042 Fragments arranged by size: 2042 1329 1047 648 359 253 246 203 159 84 MspA1I CmG'CkG Cuts at: 1606 2527 3245 3786 4463 5611 5856 1606 Size: 921 718 541 677 1148 245 2120 Fragments arranged by size: 2120 1148 921 718 677 541 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5818 NdeI CA'TA_TG Cuts at: 387 387 Size: 6370 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6370 NotI GC'GGCC_GC Cuts at: 3817 3817 Size: 6370 NspI r_CATG'y Cuts at: 1132 1229 2202 2395 2404 2983 2998 3728 5273 Size: 97 973 193 9 579 15 730 1545 Cuts at: 5273 1132 Size: 2229 Fragments arranged by size: 2229 1545 973 730 579 193 97 15 9 PasI CC'CwG_GG Cuts at: 1348 2729 1348 Size: 1381 4989 PciI A'CATG_T Cuts at: 5269 5269 Size: 6370 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6370 PfoI T'CCnGG_A Cuts at: 2340 2421 2508 3459 2340 Size: 81 87 951 5251 Fragments arranged by size: 5251 951 87 81 PmeI GTTT'AAAC Cuts at: 3674 3674 Size: 6370 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4439 4471 5952 5984 4439 Size: 32 1481 32 4825 Fragments arranged by size: 4825 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2425 2661 2425 Size: 236 6134 PshAI GACnn'nnGTC Cuts at: 1613 2666 3519 1613 Size: 1053 853 4464 Fragments arranged by size: 4464 1053 853 PsiI TTA'TAA Cuts at: 3940 3940 Size: 6370 PstI C_TGCA'G Cuts at: 830 3136 3598 3657 3722 830 Size: 2306 462 59 65 3478 Fragments arranged by size: 3478 2306 462 65 59 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6370 PvuII CAG'CTG Cuts at: 2527 2527 Size: 6370 SacI G_AGCT'C Cuts at: 2340 2340 Size: 6370 SalI G'TCGA_C Cuts at: 1936 3712 1936 Size: 1776 4594 SbfI CC_TGCA'GG Cuts at: 3722 3722 Size: 6370 ScaI AGT'ACT Cuts at: 4618 6330 4618 Size: 1712 4658 SfcI C'TryA_G Cuts at: 826 1045 3132 3594 3653 3718 4853 5534 5725 Size: 219 2087 462 59 65 1135 681 191 Cuts at: 5725 826 Size: 1471 Fragments arranged by size: 2087 1471 1135 681 462 219 191 65 59 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6370 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6370 SmaI CCC'GGG Cuts at: 1872 3697 1872 Size: 1825 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2173 2594 4474 5375 5637 820 Size: 197 926 230 421 1880 901 262 1553 Fragments arranged by size: 1880 1553 926 901 421 262 230 197 SnaBI TAC'GTA Cuts at: 493 4161 493 Size: 3668 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6370 SphI G_CATG'C Cuts at: 1132 2395 2998 3728 1132 Size: 1263 603 730 3774 Fragments arranged by size: 3774 1263 730 603 SspI AAT'ATT Cuts at: 5 52 4294 5 Size: 47 4242 2081 Fragments arranged by size: 4242 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6370 StyI C'CwwG_G Cuts at: 513 1065 2121 2712 3809 513 Size: 552 1056 591 1097 3074 Fragments arranged by size: 3074 1097 1056 591 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2923 3492 4397 4414 4567 4752 5197 Size: 1255 1343 569 905 17 153 185 445 Cuts at: 5197 325 Size: 1498 Fragments arranged by size: 1498 1343 1255 905 569 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2397 2760 4616 Size: 274 80 33 51 1443 420 363 1856 Cuts at: 4616 6328 96 Size: 1712 138 Fragments arranged by size: 1856 1712 1443 420 363 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3484 3503 3516 3535 6330 6362 3484 Size: 19 13 19 2795 32 3492 Fragments arranged by size: 3492 2795 32 19 19 13 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6370 XbaI T'CTAG_A Cuts at: 3706 3706 Size: 6370 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6370 XmaI C'CCGG_G Cuts at: 1870 3695 1870 Size: 1825 4545 XmnI GAAnn'nnTTC Cuts at: 4499 4499 Size: 6370 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4178 276 Size: 53 83 186 936 2644 2468 Fragments arranged by size: 2644 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BstZ17I EcoNI EcoRV FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI