(Linear) (Six-base) MAPSORT of: KIEE6770.seq Check: 694 from: 1 to: 1665 KIEE6770.orf With 182 enzymes: SgfI * March 16, 2015 11:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 984 1639 1665 Size: 984 655 26 Fragments arranged by size: 984 655 26 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 528 640 980 1665 Size: 528 112 340 685 Fragments arranged by size: 685 528 340 112 AflII C'TTAA_G Cuts at: 0 168 1665 Size: 168 1497 AflIII A'CryG_T Cuts at: 0 1084 1511 1665 Size: 1084 427 154 Fragments arranged by size: 1084 427 154 AleI CACnn'nnGTG Cuts at: 0 292 545 1665 Size: 292 253 1120 Fragments arranged by size: 1120 292 253 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1016 1665 Size: 1016 649 AlwNI CAG_nnn'CTG Cuts at: 0 672 1069 1656 1665 Size: 672 397 587 9 Fragments arranged by size: 672 587 397 9 ApoI r'AATT_y Cuts at: 0 244 310 319 402 1074 1665 Size: 244 66 9 83 672 591 Fragments arranged by size: 672 591 244 83 66 9 AvaI C'yCGr_G Cuts at: 0 1339 1665 Size: 1339 326 BamHI G'GATC_C Cuts at: 0 1560 1665 Size: 1560 105 BanI G'GyrC_C Cuts at: 0 112 1665 Size: 112 1553 BanII G_rGCy'C Cuts at: 0 335 498 1319 1665 Size: 335 163 821 346 Fragments arranged by size: 821 346 335 163 BbsI GAAGACnn'nnnn_ Cuts at: 0 221 405 557 1665 Size: 221 184 152 1108 Fragments arranged by size: 1108 221 184 152 BciVI GTATCCnnnnn_n' Cuts at: 0 742 1665 Size: 742 923 BglII A'GATC_T Cuts at: 0 1051 1665 Size: 1051 614 BlpI GC'TnA_GC Cuts at: 0 1062 1665 Size: 1062 603 Bme1580I G_kGCm'C Cuts at: 0 611 741 1246 1277 1665 Size: 611 130 505 31 388 Fragments arranged by size: 611 505 388 130 31 BmgBI CAC'GTC Cuts at: 0 606 1247 1512 1665 Size: 606 641 265 153 Fragments arranged by size: 641 606 265 153 BmrI ACTGGGnnnn_n' Cuts at: 0 461 1665 Size: 461 1204 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1599 1665 Size: 1599 66 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 358 490 925 1025 1665 Size: 358 132 435 100 640 Fragments arranged by size: 640 435 358 132 100 Bpu10I CC'TnA_GC Cuts at: 0 1482 1665 Size: 1482 183 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 574 1665 Size: 574 1091 BsaI GGTCTCn'nnnn_ Cuts at: 0 1628 1665 Size: 1628 37 BsaWI w'CCGG_w Cuts at: 0 1568 1665 Size: 1568 97 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1479 1509 1516 1546 1665 Size: 1479 30 7 30 119 Fragments arranged by size: 1479 119 30 30 7 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1270 1665 Size: 1270 395 BseYI C'CCAG_C Cuts at: 0 1595 1665 Size: 1595 70 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 183 1665 Size: 183 1482 BsiEI CG_ry'CG Cuts at: 0 1569 1665 Size: 1569 96 BsiHKAI G_wGCw'C Cuts at: 0 335 1665 Size: 335 1330 BsiWI C'GTAC_G Cuts at: 0 107 1665 Size: 107 1558 BsmI GAATG_Cn' Cuts at: 0 348 1267 1665 Size: 348 919 398 Fragments arranged by size: 919 398 348 BsmBI CGTCTCn'nnnn_ Cuts at: 0 596 1665 Size: 596 1069 Bsp1286I G_dGCh'C Cuts at: 0 335 498 611 741 1246 1277 1319 1665 Size: 335 163 113 130 505 31 42 346 Fragments arranged by size: 505 346 335 163 130 113 42 31 BspHI T'CATG_A Cuts at: 0 743 818 1304 1665 Size: 743 75 486 361 Fragments arranged by size: 743 486 361 75 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 984 1044 1639 1665 Size: 984 60 595 26 Fragments arranged by size: 984 595 60 26 BsrBI CCG'CTC Cuts at: 0 1061 1665 Size: 1061 604 BsrDI GCAATG_nn' Cuts at: 0 644 887 1446 1665 Size: 644 243 559 219 Fragments arranged by size: 644 559 243 219 BsrFI r'CCGG_y Cuts at: 0 1145 1665 Size: 1145 520 BsrGI T'GTAC_A Cuts at: 0 392 1665 Size: 392 1273 BstAPI GCAn_nnn'nTGC Cuts at: 0 1499 1665 Size: 1499 166 BstEII G'GTnAC_C Cuts at: 0 630 1665 Size: 630 1035 BstXI CCAn_nnnn'nTGG Cuts at: 0 210 1665 Size: 210 1455 BstYI r'GATC_y Cuts at: 0 1051 1560 1665 Size: 1051 509 105 Fragments arranged by size: 1051 509 105 Bsu36I CC'TnA_GG Cuts at: 0 182 1106 1665 Size: 182 924 559 Fragments arranged by size: 924 559 182 BtgI C'CryG_G Cuts at: 0 1520 1665 Size: 1520 145 BtsI GCAGTG_nn' Cuts at: 0 1500 1562 1645 1665 Size: 1500 62 83 20 Fragments arranged by size: 1500 83 62 20 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1509 1544 1665 Size: 1509 35 121 Fragments arranged by size: 1509 121 35 DraIII CAC_nnn'GTG Cuts at: 0 860 1665 Size: 860 805 DrdI GACnn_nn'nnGTC Cuts at: 0 1150 1665 Size: 1150 515 EaeI y'GGCC_r Cuts at: 0 1602 1665 Size: 1602 63 EarI CTCTTCn'nnn_ Cuts at: 0 257 430 1590 1665 Size: 257 173 1160 75 Fragments arranged by size: 1160 257 173 75 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 82 88 148 1665 Size: 82 6 60 1517 Fragments arranged by size: 1517 82 60 6 EcoICRI GAG'CTC Cuts at: 0 333 1665 Size: 333 1332 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 358 490 528 640 925 980 1025 1665 Size: 358 132 38 112 285 55 45 640 Fragments arranged by size: 640 358 285 132 112 55 45 38 EcoO109I rG'GnC_Cy Cuts at: 0 420 656 791 1094 1548 1665 Size: 420 236 135 303 454 117 Fragments arranged by size: 454 420 303 236 135 117 EcoRI G'AATT_C Cuts at: 0 402 1665 Size: 402 1263 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 846 1665 Size: 846 819 FspI TGC'GCA Cuts at: 0 283 1665 Size: 283 1382 FspAI rTGC'GCAy Cuts at: 0 283 1665 Size: 283 1382 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 516 548 1320 1352 1380 1412 1665 Size: 516 32 772 32 28 32 253 Fragments arranged by size: 772 516 253 32 32 32 28 HindIII A'AGCT_T Cuts at: 0 1137 1665 Size: 1137 528 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1274 1665 Size: 1274 391 MscI TGG'CCA Cuts at: 0 1604 1665 Size: 1604 61 MslI CAynn'nnrTG Cuts at: 0 208 292 545 748 994 1665 Size: 208 84 253 203 246 671 Fragments arranged by size: 671 253 246 208 203 84 MspA1I CmG'CkG Cuts at: 0 522 1240 1665 Size: 522 718 425 Fragments arranged by size: 718 522 425 NspI r_CATG'y Cuts at: 0 197 390 399 978 993 1665 Size: 197 193 9 579 15 672 Fragments arranged by size: 672 579 197 193 15 9 PasI CC'CwG_GG Cuts at: 0 724 1665 Size: 724 941 PfoI T'CCnGG_A Cuts at: 0 335 416 503 1454 1665 Size: 335 81 87 951 211 Fragments arranged by size: 951 335 211 87 81 PpuMI rG'GwC_Cy Cuts at: 0 420 656 1665 Size: 420 236 1009 Fragments arranged by size: 1009 420 236 PshAI GACnn'nnGTC Cuts at: 0 661 1514 1665 Size: 661 853 151 Fragments arranged by size: 853 661 151 PstI C_TGCA'G Cuts at: 0 1131 1593 1652 1665 Size: 1131 462 59 13 Fragments arranged by size: 1131 462 59 13 PvuII CAG'CTG Cuts at: 0 522 1665 Size: 522 1143 SacI G_AGCT'C Cuts at: 0 335 1665 Size: 335 1330 SfcI C'TryA_G Cuts at: 0 1127 1589 1648 1665 Size: 1127 462 59 17 Fragments arranged by size: 1127 462 59 17 SmlI C'TyrA_G Cuts at: 0 168 589 1665 Size: 168 421 1076 Fragments arranged by size: 1076 421 168 SphI G_CATG'C Cuts at: 0 390 993 1665 Size: 390 603 672 Fragments arranged by size: 672 603 390 StyI C'CwwG_G Cuts at: 0 116 707 1665 Size: 116 591 958 Fragments arranged by size: 958 591 116 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 918 1487 1665 Size: 918 569 178 Fragments arranged by size: 918 569 178 TatI w'GTAC_w Cuts at: 0 392 755 1665 Size: 392 363 910 Fragments arranged by size: 910 392 363 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1479 1498 1511 1530 1665 Size: 1479 19 13 19 135 Fragments arranged by size: 1479 135 19 19 13 Enzymes that do cut: AarI AcuI AflII AflIII AleI AlfI AlwNI ApoI AvaI BamHI BanI BanII BbsI BciVI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI Hin4I HindIII MmeI MscI MslI MspA1I NspI PasI PfoI PpuMI PshAI PstI PvuII SacI SfcI SmlI SphI StyI TaqII TatI TstI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AgeI AhdI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BclI BfrBI BglI BmtI BsaAI BsaBI BsaHI BspEI BssHII BssSI BstBI BstZ17I BtgZI ClaI DraI EagI EcoNI EcoRV FseI HaeII HincII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PflMI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI