Restriction map for
pFN21AE1144
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccI AccII AccIII
AcyI AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AvaII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaJI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsoBI Bsp1407I
BspDI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
Cfr13I ClaI CpoI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FseI HaeII HgaI Hin1I
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MflI MluI
MroI MslI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI PvuI RcaI RsrII SacI
SacII SalI SapI Sau96I SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspBI StuI
StyI Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AclI 1067 AACGTT AA^CGTT
AflIII 744 ACATGT A^C[GA][CT]GT
AlwI 556 GATCC GGATC(4/5)
AviII 764 TGCGCA TGC^GCA
BalI 578 TGGCCA TGG^CCA
BanII 167 GGGCTC G[GA]GC[CT]^C
BciVI 644 GTATCC GTATCC(6/5)
BcnI 165 CCGGG CC^[GC]GG
BmyI 167 GGGCTC G[AGT]GC[ACT]^C
BseRI 8 GAGGAG GAGGAG(10/8)
BsgI 485 CTGCAC GTGCAG(16/14)
BsmI 87 GCATTC GAATGC(1/-1)
Bsp1286I 167 GGGCTC G[AGT]GC[ACT]^C
BspLU11I 744 ACATGT A^CATGT
BsrDI 1059 CATTGC GCAATG(2/0)
Cac8I 255 GCCAGC GC[ACGT]^[ACGT]GC
CfoI 765 GCGC GCG^C
FspI 764 TGCGCA TGC^GCA
HapII 165 CCGG C^CGG
HhaI 765 GCGC GCG^C
HinP1I 765 GCGC G^CGC
HindIII 450 AAGCTT A^AGCTT
HpaII 165 CCGG C^CGG
HphI 850 TCACC GGTGA(8/7)
MaeII 1068 ACGT A^CGT
MluNI 578 TGGCCA TGG^CCA
MscI 578 TGGCCA TGG^CCA
MspI 165 CCGG C^CGG
MwoI 370 GCTGAAGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 165 CCGGG CC^[GC]GG
NlaIV 197 GGAACC GG[ACGT]^[ACGT]CC
Psp1406I 1067 AACGTT AA^CGTT
PstI 50 CTGCAG CTGCA^G
SexAI 200 ACCAGGT A^CC[AT]GGT
SfcI 50 CTGCAG C^T[GA][CT]AG
SspI 146 AATATT AAT^ATT
SwaI 141 ATTTAAAT ATTT^AAAT
XcmI 933 CCATCTGTACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AciI 189 CCGC CCGC(-3/-1)
1155 CCGC
AflII 529 CTTAAG C^TTAAG
675 CTTAAG
BbsI 522 GTCTTC GAAGAC(2/6)
732 GTCTTC
BclI 293 TGATCA T^GATCA
660 TGATCA
BfrI 529 CTTAAG C^TTAAG
675 CTTAAG
BpmI 545 CTCCAG CTGGAG(16/14)
635 CTCCAG
BpuAI 522 GTCTTC GAAGAC(2/6)
732 GTCTTC
BsiYI 648 CCTTTGTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
993 CCCTAGACTGG
BslI 648 CCTTTGTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
993 CCCTAGACTGG
BspMI 924 ACCTGC ACCTGC(4/8)
640 GCAGGT
BstF5I 283 CATCC GGATG(2/0)
831 GGATG
BstNI 201 CCAGG CC^[AT]GG
790 CCTGG
DraI 142 TTTAAA TTT^AAA
414 TTTAAA
EcoRII 201 CCAGG ^CC[AT]GG
790 CCTGG
FbaI 293 TGATCA T^GATCA
660 TGATCA
FokI 283 CATCC GGATG(9/13)
831 GGATG
MspA1I 368 CAGCTG C[AC]G^C[GT]G
618 CAGCTG
MvaI 201 CCAGG CC^[AT]GG
790 CCTGG
PvuII 368 CAGCTG CAG^CTG
618 CAGCTG
ScaI 570 AGTACT AGT^ACT
1185 AGTACT
SpeI 573 ACTAGT A^CTAGT
1008 ACTAGT
TaqI 444 TCGA T^CGA
750 TCGA
Tsp45I 23 GTGAC ^GT[GC]AC
668 GTCAC
TthHB8I 444 TCGA T^CGA
750 TCGA
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# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbvI
BfaI BsmAI BsrI BssKI DdeI
DpnII EaeI EarI Fnu4HI HaeIII
HinfI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI NdeII
NlaIII NspI RsaI Sau3AI ScrFI
SfaNI SmlI TfiI Tru9I TseI
Tsp509I TspRI