(Circular) (Six-base) MAPSORT of: pFN21AE1144.seq Check: 6172 from: 1 to: 5989 pFN21AE1144 5989 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3799 278 Size: 53 83 186 936 2263 2468 Fragments arranged by size: 2468 2263 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3332 1937 Size: 1395 4594 Acc65I G'GTAC_C Cuts at: 3310 3310 Size: 5989 AclI AA'CG_TT Cuts at: 1563 3073 4116 4489 1563 Size: 1510 1043 373 3063 Fragments arranged by size: 3063 1510 1043 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2108 2397 4052 5436 5704 1632 Size: 476 289 1655 1384 268 1917 Fragments arranged by size: 1917 1655 1384 476 289 268 AflII C'TTAA_G Cuts at: 820 1017 2534 2680 820 Size: 197 1517 146 4129 Fragments arranged by size: 4129 1517 197 146 AflIII A'CryG_T Cuts at: 1203 2749 4888 5865 1203 Size: 1546 2139 977 1327 Fragments arranged by size: 2139 1546 1327 977 AgeI A'CCGG_T Cuts at: 4820 4820 Size: 5989 AhdI GACnn_n'nnGTC Cuts at: 4718 4718 Size: 5989 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5989 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2987 3091 1184 Size: 1803 104 4082 Fragments arranged by size: 4082 1803 104 AlwNI CAG_nnn'CTG Cuts at: 3732 5304 3732 Size: 1572 4417 ApaLI G'TGCA_C Cuts at: 4046 5202 4046 Size: 1156 4833 ApoI r'AATT_y Cuts at: 1493 2243 2900 3082 3298 3524 3676 5785 1493 Size: 750 657 182 216 226 152 2109 1697 Fragments arranged by size: 2109 1697 750 657 226 216 182 152 AseI AT'TA_AT Cuts at: 160 1032 4543 160 Size: 872 3511 1606 Fragments arranged by size: 3511 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5989 AvaI C'yCGr_G Cuts at: 1870 1943 3314 1870 Size: 73 1371 4545 Fragments arranged by size: 4545 1371 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2538 2571 2538 Size: 33 5956 BamHI G'GATC_C Cuts at: 3319 3686 3319 Size: 367 5622 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3310 4765 618 Size: 325 217 627 1523 1455 1842 Fragments arranged by size: 1842 1523 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2176 3308 1391 Size: 785 1132 4072 Fragments arranged by size: 4072 1132 785 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2520 2730 928 Size: 971 621 210 4187 Fragments arranged by size: 4187 971 621 210 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4180 4214 1903 Size: 34 2243 34 3678 Fragments arranged by size: 3678 2243 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2660 3881 5091 2660 Size: 1221 1210 3558 Fragments arranged by size: 3558 1221 1210 BclI T'GATC_A Cuts at: 1548 2298 2665 1548 Size: 750 367 4872 Fragments arranged by size: 4872 750 367 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4600 136 Size: 107 122 71 631 605 2928 1525 Fragments arranged by size: 2928 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5984 5984 Size: 5989 BlpI GC'TnA_GC Cuts at: 3406 3406 Size: 5989 Bme1580I G_kGCm'C Cuts at: 1790 4050 5206 1790 Size: 2260 1156 2573 Fragments arranged by size: 2573 2260 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5989 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3241 3720 4678 Size: 416 53 83 399 24 1824 479 958 Cuts at: 4678 442 Size: 1753 Fragments arranged by size: 1824 1753 958 479 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5989 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2535 2625 4649 1239 Size: 1296 90 2024 2579 Fragments arranged by size: 2579 2024 1296 90 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2588 4114 4979 5277 2588 Size: 1526 865 298 3300 Fragments arranged by size: 3300 1526 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4652 882 Size: 617 3153 2219 Fragments arranged by size: 3153 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3780 493 Size: 711 2576 2702 Fragments arranged by size: 2702 2576 711 BsaBI GATnn'nnATC Cuts at: 5983 5983 Size: 5989 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3796 4178 4813 275 Size: 53 83 186 936 2263 382 635 1451 Fragments arranged by size: 2263 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4422 4820 5094 5241 5652 4422 Size: 398 274 147 411 4759 Fragments arranged by size: 4759 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5764 Fragments arranged by size: 5764 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2028 1186 Size: 842 5147 BseYI C'CCAG_C Cuts at: 2832 5192 5688 2832 Size: 2360 496 3133 Fragments arranged by size: 3133 2360 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2475 1163 Size: 573 739 4677 Fragments arranged by size: 4677 739 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3439 4200 5228 1540 Size: 275 186 1438 761 1028 2301 Fragments arranged by size: 2301 1438 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4050 4135 5206 4050 Size: 85 1071 4833 Fragments arranged by size: 4833 1071 85 BsmI GAATG_Cn' Cuts at: 2092 3500 3593 2092 Size: 1408 93 4488 Fragments arranged by size: 4488 1408 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2176 3308 4050 4135 5206 1391 Size: 399 386 1132 742 85 1071 2174 Fragments arranged by size: 2174 1132 1071 742 399 386 85 BspEI T'CCGG_A Cuts at: 5652 5652 Size: 5989 BspHI T'CATG_A Cuts at: 3878 5608 3878 Size: 1730 4259 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2636 2938 3344 844 Size: 1792 302 406 3489 Fragments arranged by size: 3489 1792 406 302 BsrBI CCG'CTC Cuts at: 1394 1823 3876 1394 Size: 429 2053 3507 Fragments arranged by size: 3507 2053 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3063 4484 4658 65 Size: 1183 1815 1421 174 1396 Fragments arranged by size: 1815 1421 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4633 4820 1700 Size: 110 2823 187 2869 Fragments arranged by size: 2869 2823 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5989 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5989 BssSI C'ACGA_G Cuts at: 4049 5061 4049 Size: 1012 4977 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5989 BstBI TT'CG_AA Cuts at: 4793 4793 Size: 5989 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5989 BstYI r'GATC_y Cuts at: 1972 3319 3686 4071 4088 5540 5626 5638 5984 Size: 1347 367 385 17 1452 86 12 346 Cuts at: 5984 1972 Size: 1977 Fragments arranged by size: 1977 1452 1347 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5437 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5250 Fragments arranged by size: 5250 683 56 BtsI GCAGTG_nn' Cuts at: 3505 4299 4319 3505 Size: 794 20 5175 Fragments arranged by size: 5175 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5989 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5954 DraI TTT'AAA Cuts at: 2149 2421 3293 2149 Size: 272 872 4845 Fragments arranged by size: 4845 872 272 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4996 809 Size: 507 3680 1802 Fragments arranged by size: 3680 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2583 2977 3038 Size: 54 1425 50 275 18 753 394 61 Cuts at: 3038 3157 3436 4325 5954 8 Size: 119 279 889 1629 43 Fragments arranged by size: 1629 1425 889 753 394 279 275 119 61 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3436 1537 Size: 275 1624 4090 Fragments arranged by size: 4090 1624 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2014 2166 2819 3045 3919 1353 Size: 661 152 653 226 874 3423 Fragments arranged by size: 3423 874 661 653 226 152 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4576 4950 5096 387 Size: 1064 138 2987 374 146 1280 Fragments arranged by size: 2987 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2108 2397 2535 2625 4052 4649 5436 Size: 393 476 289 138 90 1427 597 787 Cuts at: 5436 5704 1239 Size: 268 1524 Fragments arranged by size: 1524 1427 787 597 476 393 289 268 138 90 EcoRI G'AATT_C Cuts at: 3298 3676 3298 Size: 378 5611 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2497 2990 2497 Size: 493 5496 FspI TGC'GCA Cuts at: 2771 4495 2771 Size: 1724 4265 HaeII r_GCGC'y Cuts at: 1678 5136 1678 Size: 3458 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2381 2413 2549 2581 2611 Size: 32 135 32 809 32 136 32 30 Cuts at: 2611 2643 4630 4662 4704 4736 1373 Size: 32 1987 32 42 32 2626 Fragments arranged by size: 2626 1987 809 136 135 42 32 32 32 32 32 32 32 30 HincII GTy'rAC Cuts at: 669 1657 1938 3333 3579 669 Size: 988 281 1395 246 3079 Fragments arranged by size: 3079 1395 988 281 246 HindIII A'AGCT_T Cuts at: 748 2455 748 Size: 1707 4282 HpaI GTT'AAC Cuts at: 3579 3579 Size: 5989 KpnI G_GTAC'C Cuts at: 3314 3314 Size: 5989 MfeI C'AATT_G Cuts at: 3588 3588 Size: 5989 MluI A'CGCG_T Cuts at: 5865 5865 Size: 5989 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5103 5287 1305 Size: 3798 184 2007 Fragments arranged by size: 3798 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2585 10 Size: 54 1425 343 753 3414 Fragments arranged by size: 3414 1425 753 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3947 4306 4465 518 Size: 648 2781 359 159 2042 Fragments arranged by size: 2781 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2375 2625 3405 4082 5230 5475 1606 Size: 769 250 780 677 1148 245 2120 Fragments arranged by size: 2120 1148 780 769 677 250 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5437 NdeI CA'TA_TG Cuts at: 387 387 Size: 5989 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5989 NotI GC'GGCC_GC Cuts at: 3436 3436 Size: 5989 NspI r_CATG'y Cuts at: 1132 1229 2221 2753 2997 3347 4892 1132 Size: 97 992 532 244 350 1545 2229 Fragments arranged by size: 2229 1545 992 532 350 244 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5989 PciI A'CATG_T Cuts at: 2749 4888 2749 Size: 2139 3850 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5989 PfoI T'CCnGG_A Cuts at: 2794 2794 Size: 5989 PmeI GTTT'AAAC Cuts at: 3293 3293 Size: 5989 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4058 4090 5571 5603 4058 Size: 32 1481 32 4444 Fragments arranged by size: 4444 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5989 PsiI TTA'TAA Cuts at: 3559 3559 Size: 5989 PstI C_TGCA'G Cuts at: 830 2059 3341 830 Size: 1229 1282 3478 Fragments arranged by size: 3478 1282 1229 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5989 PvuII CAG'CTG Cuts at: 2375 2625 2375 Size: 250 5739 SalI G'TCGA_C Cuts at: 1936 3331 1936 Size: 1395 4594 SbfI CC_TGCA'GG Cuts at: 3341 3341 Size: 5989 ScaI AGT'ACT Cuts at: 2577 3192 4237 5949 2577 Size: 615 1045 1712 2617 Fragments arranged by size: 2617 1712 1045 615 SexAI A'CCwGG_T Cuts at: 2205 2205 Size: 5989 SfcI C'TryA_G Cuts at: 826 1045 2055 3337 4472 5153 5344 826 Size: 219 1010 1282 1135 681 191 1471 Fragments arranged by size: 1471 1282 1135 1010 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5989 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5989 SmaI CCC'GGG Cuts at: 1872 3316 1872 Size: 1444 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2534 2603 2680 4093 4994 5256 Size: 197 926 591 69 77 1413 901 262 Cuts at: 5256 820 Size: 1553 Fragments arranged by size: 1553 1413 926 901 591 262 197 77 69 SnaBI TAC'GTA Cuts at: 493 3780 493 Size: 3287 2702 SpeI A'CTAG_T Cuts at: 152 2578 3013 152 Size: 2426 435 3128 Fragments arranged by size: 3128 2426 435 SphI G_CATG'C Cuts at: 1132 3347 1132 Size: 2215 3774 SspI AAT'ATT Cuts at: 5 52 2153 3913 5 Size: 47 2101 1760 2081 Fragments arranged by size: 2101 2081 1760 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5989 StyI C'CwwG_G Cuts at: 513 1065 3428 513 Size: 552 2363 3074 Fragments arranged by size: 3074 2363 552 SwaI ATTT'AAAT Cuts at: 2149 2149 Size: 5989 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2872 2951 4016 4033 4186 4371 4816 Size: 1255 1292 79 1065 17 153 185 445 Cuts at: 4816 325 Size: 1498 Fragments arranged by size: 1498 1292 1255 1065 445 185 153 79 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2575 2943 3190 Size: 274 80 33 51 1443 598 368 247 Cuts at: 3190 4235 5947 96 Size: 1045 1712 138 Fragments arranged by size: 1712 1443 1045 598 368 274 247 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5949 5981 5949 Size: 32 5957 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5989 XbaI T'CTAG_A Cuts at: 3325 3325 Size: 5989 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2945 2945 Size: 5989 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5989 XmaI C'CCGG_G Cuts at: 1870 3314 1870 Size: 1444 4545 XmnI GAAnn'nnTTC Cuts at: 4118 4118 Size: 5989 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3797 276 Size: 53 83 186 936 2263 2468 Fragments arranged by size: 2468 2263 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI