################################## # NO CUT ENZYMES ( 161 enzymes) # ################################## AatII AccIII AclI AcyI AflII AflIII AgeI AhdI AluI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspHI AspI AvaII AviII AvrII BalI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI Hin1I HindIII HpaI HpaII KasI KspI MaeII MluI MluNI MroI MscI MspI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### Acc65I 132 GGTACC G^GTACC AccI 71 GTCTAC GT^[AC][GT]AC AccII 643 CGCG CG^CG AcsI 361 AAATTC [GA]^AATT[CT] AfaI 133 GTAC GT^AC ApoI 361 AAATTC [GA]^AATT[CT] Asp700I 357 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 132 GGTACC G^GTACC AvaI 37 CCCGAG C^[CT]CG[GA]G BamHI 111 GGATCC G^GATCC BanII 387 GAGCCC G[GA]GC[CT]^C BfaI 587 CTAG C^TAG BglII 498 AGATCT A^GATCT BsaBI 529 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 445 GAGACC GGTCTC(1/5) BsgI 149 GTGCAG GTGCAG(16/14) BsiYI 123 CCTTAGCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 123 CCTTAGCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 445 GAGAC GTCTC(1/5) BsmFI 228 GGGAC GGGAC(10/14) BsoBI 37 CCCGAG C^[CT]CG[GA]G BsrDI 275 GCAATG GCAATG(2/0) BstEII 42 GGTCACC G^GT[ACGT]ACC BstPI 42 GGTCACC G^GT[ACGT]ACC BstUI 643 CGCG CG^CG BstXI 404 CCAGATACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 14 GCTGGC GC[ACGT]^[ACGT]GC CfoI 642 GCGC GCG^C DsaI 576 CCATGG C^C[GA][CT]GG EarI 202 GAAGAG CTCTTC(1/4) EcoO65I 42 GGTCACC G^GT[ACGT]ACC EcoT14I 576 CCATGG C^C[AT][AT]GG HaeIII 30 GGCC GG^CC HhaI 642 GCGC GCG^C HinP1I 642 GCGC G^CGC HincII 8 GTCAAC GT[CT]^[GA]AC HindII 8 GTCAAC GT[CT]^[GA]AC KpnI 132 GGTACC GGTAC^C Ksp632I 202 GAAGAG CTCTTC(1/4) MaeI 587 CTAG C^TAG MamI 529 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 290 CAATTG C^AATTG MspA1I 22 CCGCTG C[AC]G^C[GT]G MunI 290 CAATTG C^AATTG MvnI 643 CGCG CG^CG MwoI 676 GCTCTTTCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 576 CCATGG C^CATGG PstI 683 CTGCAG CTGCA^G RsaI 133 GTAC GT^AC SfcI 683 CTGCAG C^T[GA][CT]AG SpeI 586 ACTAGT A^CTAGT StyI 576 CCATGG C^C[AT][AT]GG Tsp45I 43 GTCAC ^GT[GC]AC TspRI 450 CAGTG CA[GC]TG[ACGT][ACGT]^ XmnI 357 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AciI 22 CCGC CCGC(-3/-1) 555 GCGG BanI 132 GGTACC G^G[CT][GA]CC 474 GGCACC BbvI 685 GCAGC GCAGC(8/12) 18 GCTGC BmyI 387 GAGCCC G[AGT]GC[ACT]^C 452 GTGCCC BsaJI 38 CCGAGG C^C[ACGT][ACGT]GG 576 CCATGG Bsp1286I 387 GAGCCC G[AGT]GC[ACT]^C 452 GTGCCC BspHI 257 TCATGA T^CATGA 441 TCATGA BssKI 366 CCTGG ^CC[ACGT]GG 411 CCTGG BstNI 366 CCTGG CC^[AT]GG 411 CCTGG BstYI 111 GGATCC [GA]^GATC[CT] 498 AGATCT EcoRII 366 CCTGG ^CC[AT]GG 411 CCTGG HphI 580 GGTGA GGTGA(8/7) 44 TCACC MflI 111 GGATCC [GA]^GATC[CT] 498 AGATCT MslI 523 CATGTAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 535 CATCTCTATG MvaI 366 CCTGG CC^[AT]GG 411 CCTGG RcaI 257 TCATGA T^CATGA 441 TCATGA ScrFI 366 CCTGG CC^[ACGT]GG 411 CCTGG TseI 18 GCTGC G^C[AT]GC 685 GCAGC XhoII 111 GGATCC [GA]^GATC[CT] 498 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AlwI BpmI BsrI BstF5I DdeI DpnII Fnu4HI FokI HinfI ItaI MaeIII MboI MboII MnlI MseI NdeII NlaIII NlaIV Sau3AI SfaNI TfiI Tru9I Tsp509I