(Linear) (Six-base) MAPSORT of: KIEE3072.seq  Check: 8961  from: 1  to: 690



KIEE3072.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:10  ..

AccI GT'mk_AC

Cuts at:      0      72     690 
   Size:         72     618
 
Acc65I G'GTAC_C

Cuts at:      0     132     690 
   Size:        132     558
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     413     690 
   Size:        413     277
 
ApoI r'AATT_y

Cuts at:      0     361     690 
   Size:        361     329
 
AvaI C'yCGr_G

Cuts at:      0      37     690 
   Size:         37     653
 
BamHI G'GATC_C

Cuts at:      0     111     690 
   Size:        111     579
 
BanI G'GyrC_C

Cuts at:      0     132     474     690 
   Size:        132     342     216

  Fragments arranged by size: 

                 342     216     132
 
BanII G_rGCy'C

Cuts at:      0     391     690 
   Size:        391     299
 
BglII A'GATC_T

Cuts at:      0     498     690 
   Size:        498     192
 
Bme1580I G_kGCm'C

Cuts at:      0     456     690 
   Size:        456     234
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     388     433     690 
   Size:         46     342      45     257

  Fragments arranged by size: 

                 342     257      46      45
 
Bpu10I CC'TnA_GC

Cuts at:      0     124     690 
   Size:        124     566
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     439     690 
   Size:        439     251
 
BsaBI GATnn'nnATC

Cuts at:      0     533     690 
   Size:        533     157
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     170     690 
   Size:        170     520
 
Bsp1286I G_dGCh'C

Cuts at:      0     391     456     690 
   Size:        391      65     234

  Fragments arranged by size: 

                 391     234      65
 
BspHI T'CATG_A

Cuts at:      0     257     441     690 
   Size:        257     184     249

  Fragments arranged by size: 

                 257     249     184
 
BsrDI GCAATG_nn'

Cuts at:      0     282     690 
   Size:        282     408
 
BstEII G'GTnAC_C

Cuts at:      0      42     690 
   Size:         42     648
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     411     690 
   Size:        411     279
 
BstYI r'GATC_y

Cuts at:      0     111     498     690 
   Size:        111     387     192

  Fragments arranged by size: 

                 387     192     111
 
BtgI C'CryG_G

Cuts at:      0     576     690 
   Size:        576     114
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     264     299     690 
   Size:        264      35     391

  Fragments arranged by size: 

                 391     264      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     197     690 
   Size:        197     493
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     570     690 
   Size:        570     120
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     388     413     433     690 
   Size:         46     342      25      20     257

  Fragments arranged by size: 

                 342     257      46      25      20
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     351     517     690 
   Size:        351     166     173

  Fragments arranged by size: 

                 351     173     166
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     226     258     690 
   Size:        226      32     432

  Fragments arranged by size: 

                 432     226      32
 
HincII GTy'rAC

Cuts at:      0      10     690 
   Size:         10     680
 
KpnI G_GTAC'C

Cuts at:      0     136     690 
   Size:        136     554
 
MfeI C'AATT_G

Cuts at:      0     290     690 
   Size:        290     400
 
MslI CAynn'nnrTG

Cuts at:      0     527     539     690 
   Size:        527      12     151

  Fragments arranged by size: 

                 527     151      12
 
MspA1I CmG'CkG

Cuts at:      0      24     690 
   Size:         24     666
 
NcoI C'CATG_G

Cuts at:      0     576     690 
   Size:        576     114
 
PfoI T'CCnGG_A

Cuts at:      0     365     690 
   Size:        365     325
 
PstI C_TGCA'G

Cuts at:      0     687     690 
   Size:        687       3
 
SfcI C'TryA_G

Cuts at:      0     683     690 
   Size:        683       7
 
SpeI A'CTAG_T

Cuts at:      0     586     690 
   Size:        586     104
 
StyI C'CwwG_G

Cuts at:      0     576     690 
   Size:        576     114
 
XmnI GAAnn'nnTTC

Cuts at:      0     361     690 
   Size:        361     329
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     ApoI     AvaI    BamHI     BanI    BanII
    BglII Bme1580I     BpmI   Bpu10I     BsaI    BsaBI     BsgI Bsp1286I
    BspHI    BsrDI   BstEII    BstXI    BstYI     BtgI    CspCI     EarI
     EciI  Eco57MI     FalI    Hin4I   HincII     KpnI     MfeI     MslI
   MspA1I     NcoI     PfoI     PstI     SfcI     SpeI     StyI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI     BlpI    BmgBI     BmrI     BmtI     BplI
    BpuEI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI     DraI   DraIII     DrdI     EaeI     EagI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
  HindIII     HpaI     KasI     MluI     MmeI     MscI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI