(Circular) (Six-base) MAPSORT of: pFN21AE1984.seq Check: 6367 from: 1 to: 6382 pFN21AE1984 6382 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4192 278 Size: 53 83 186 936 2656 2468 Fragments arranged by size: 2656 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3725 1937 Size: 1788 4594 Acc65I G'GTAC_C Cuts at: 3703 3703 Size: 6382 AclI AA'CG_TT Cuts at: 1563 4509 4882 1563 Size: 2946 373 3063 Fragments arranged by size: 3063 2946 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2993 3391 4445 5829 6097 1632 Size: 1361 398 1054 1384 268 1917 Fragments arranged by size: 1917 1384 1361 1054 398 268 AflII C'TTAA_G Cuts at: 820 1017 2251 820 Size: 197 1234 4951 Fragments arranged by size: 4951 1234 197 AflIII A'CryG_T Cuts at: 1203 3578 5281 6258 1203 Size: 2375 1703 977 1327 Fragments arranged by size: 2375 1703 1327 977 AgeI A'CCGG_T Cuts at: 5213 5213 Size: 6382 AhdI GACnn_n'nnGTC Cuts at: 5111 5111 Size: 6382 AleI CACnn'nnGTG Cuts at: 1166 2658 3637 1166 Size: 1492 979 3911 Fragments arranged by size: 3911 1492 979 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6382 AlwNI CAG_nnn'CTG Cuts at: 4125 5697 4125 Size: 1572 4810 ApaLI G'TGCA_C Cuts at: 4439 5595 4439 Size: 1156 5226 ApoI r'AATT_y Cuts at: 1493 2168 2537 2846 2923 3691 3917 4069 6178 Size: 675 369 309 77 768 226 152 2109 Cuts at: 6178 1493 Size: 1697 Fragments arranged by size: 2109 1697 768 675 369 309 226 152 77 AseI AT'TA_AT Cuts at: 160 1032 4936 160 Size: 872 3904 1606 Fragments arranged by size: 3904 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6382 AvaI C'yCGr_G Cuts at: 1870 1943 3707 1870 Size: 73 1764 4545 Fragments arranged by size: 4545 1764 73 BamHI G'GATC_C Cuts at: 2058 3712 4079 2058 Size: 1654 367 4361 Fragments arranged by size: 4361 1654 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3703 5158 618 Size: 325 217 627 1916 1455 1842 Fragments arranged by size: 1916 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2723 3701 1391 Size: 1332 978 4072 Fragments arranged by size: 4072 1332 978 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2289 928 Size: 971 390 5021 Fragments arranged by size: 5021 971 390 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4573 4607 1903 Size: 34 2636 34 3678 Fragments arranged by size: 3678 2636 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3526 4274 5484 3526 Size: 748 1210 4424 Fragments arranged by size: 4424 1210 748 BclI T'GATC_A Cuts at: 1548 2185 2350 1548 Size: 637 165 5580 Fragments arranged by size: 5580 637 165 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4993 136 Size: 107 122 71 631 605 3321 1525 Fragments arranged by size: 3321 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6377 6377 Size: 6382 BlpI GC'TnA_GC Cuts at: 3799 3799 Size: 6382 Bme1580I G_kGCm'C Cuts at: 1790 4443 5599 1790 Size: 2653 1156 2573 Fragments arranged by size: 2653 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6382 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4113 5071 442 Size: 416 53 83 399 24 2696 958 1753 Fragments arranged by size: 2696 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6382 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3615 3615 Size: 6382 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2034 2145 2400 5042 1239 Size: 795 111 255 2642 2579 Fragments arranged by size: 2642 2579 795 255 111 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3060 3089 3585 4507 5372 5670 3060 Size: 29 496 922 865 298 3772 Fragments arranged by size: 3772 922 865 496 298 29 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3000 3329 5045 882 Size: 617 1501 329 1716 2219 Fragments arranged by size: 2219 1716 1501 617 329 BsaAI yAC'GTr Cuts at: 493 1204 4173 493 Size: 711 2969 2702 Fragments arranged by size: 2969 2702 711 BsaBI GATnn'nnATC Cuts at: 2232 6376 2232 Size: 4144 2238 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4189 4571 5206 275 Size: 53 83 186 936 2656 382 635 1451 Fragments arranged by size: 2656 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4815 5213 5487 5634 6045 4815 Size: 398 274 147 411 5152 Fragments arranged by size: 5152 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6157 Fragments arranged by size: 6157 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6382 BseYI C'CCAG_C Cuts at: 5585 6081 5585 Size: 496 5886 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5809 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3832 4593 5621 1540 Size: 275 186 1831 761 1028 2301 Fragments arranged by size: 2301 1831 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4443 4528 5599 4443 Size: 85 1071 5226 Fragments arranged by size: 5226 1071 85 BsmI GAATG_Cn' Cuts at: 3893 3986 3893 Size: 93 6289 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2723 3701 4443 4528 5599 1391 Size: 399 933 978 742 85 1071 2174 Fragments arranged by size: 2174 1071 978 933 742 399 85 BspEI T'CCGG_A Cuts at: 6045 6045 Size: 6382 BspHI T'CATG_A Cuts at: 2353 4271 6001 2353 Size: 1918 1730 2734 Fragments arranged by size: 2734 1918 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3737 844 Size: 2893 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4269 1394 Size: 429 2446 3507 Fragments arranged by size: 3507 2446 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2471 3399 4877 5051 65 Size: 1183 1223 928 1478 174 1396 Fragments arranged by size: 1478 1396 1223 1183 928 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3355 5026 5213 1700 Size: 110 1545 1671 187 2869 Fragments arranged by size: 2869 1671 1545 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6382 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6382 BssSI C'ACGA_G Cuts at: 4442 5454 4442 Size: 1012 5370 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6382 BstBI TT'CG_AA Cuts at: 5186 5186 Size: 6382 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6382 BstYI r'GATC_y Cuts at: 1972 2058 3712 4079 4464 4481 5933 6019 6031 Size: 86 1654 367 385 17 1452 86 12 Cuts at: 6031 6377 1972 Size: 346 1977 Fragments arranged by size: 1977 1654 1452 385 367 346 86 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3135 3187 513 Size: 552 939 1131 52 3708 Fragments arranged by size: 3708 1131 939 552 52 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2469 487 Size: 683 56 1243 4400 Fragments arranged by size: 4400 1243 683 56 BtsI GCAGTG_nn' Cuts at: 3460 3898 4692 4712 3460 Size: 438 794 20 5130 Fragments arranged by size: 5130 794 438 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6382 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6347 DraI TTT'AAA Cuts at: 2070 2140 3686 2070 Size: 70 1546 4766 Fragments arranged by size: 4766 1546 70 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2476 3214 5389 809 Size: 507 1160 738 2175 1802 Fragments arranged by size: 2175 1802 1160 738 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3829 4718 6347 Size: 54 1425 50 275 18 1999 889 1629 Cuts at: 6347 8 Size: 43 Fragments arranged by size: 1999 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3829 1537 Size: 275 2017 4090 Fragments arranged by size: 4090 2017 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2301 2487 4312 1353 Size: 948 186 1825 3423 Fragments arranged by size: 3423 1825 948 186 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4969 5343 5489 387 Size: 1064 138 3380 374 146 1280 Fragments arranged by size: 3380 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2034 2145 2400 2993 3391 4445 5042 Size: 393 402 111 255 593 398 1054 597 Cuts at: 5042 5829 6097 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1054 787 597 593 402 398 393 268 255 111 EcoRI G'AATT_C Cuts at: 3691 4069 3691 Size: 378 6004 FspI TGC'GCA Cuts at: 4888 4888 Size: 6382 HaeII r_GCGC'y Cuts at: 1678 5529 1678 Size: 3851 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2897 2926 2929 2958 2960 Size: 32 135 32 1325 29 3 29 2 Cuts at: 2960 2992 3110 3142 3581 3613 5023 5055 5097 Size: 32 118 32 439 32 1410 32 42 Cuts at: 5097 5129 1373 Size: 32 2626 Fragments arranged by size: 2626 1410 1325 439 135 118 42 32 32 32 32 32 32 32 29 29 3 2 HincII GTy'rAC Cuts at: 669 1657 1938 3726 3972 669 Size: 988 281 1788 246 3079 Fragments arranged by size: 3079 1788 988 281 246 HindIII A'AGCT_T Cuts at: 748 2241 748 Size: 1493 4889 HpaI GTT'AAC Cuts at: 3972 3972 Size: 6382 KpnI G_GTAC'C Cuts at: 3707 3707 Size: 6382 MfeI C'AATT_G Cuts at: 2841 3981 2841 Size: 1140 5242 MluI A'CGCG_T Cuts at: 6258 6258 Size: 6382 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2401 2643 3623 5496 5680 1305 Size: 1096 242 980 1873 184 2007 Fragments arranged by size: 2007 1873 1096 980 242 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4560 Fragments arranged by size: 4560 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2658 3485 3577 3637 4340 4699 4858 Size: 648 1492 827 92 60 703 359 159 Cuts at: 4858 518 Size: 2042 Fragments arranged by size: 2042 1492 827 703 648 359 159 92 60 MspA1I CmG'CkG Cuts at: 1606 3798 4475 5623 5868 1606 Size: 2192 677 1148 245 2120 Fragments arranged by size: 2192 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3135 3187 513 Size: 552 939 1131 52 3708 Fragments arranged by size: 3708 1131 939 552 52 NdeI CA'TA_TG Cuts at: 387 3426 387 Size: 3039 3343 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6382 NotI GC'GGCC_GC Cuts at: 3829 3829 Size: 6382 NspI r_CATG'y Cuts at: 1132 1229 3436 3582 3740 5285 1132 Size: 97 2207 146 158 1545 2229 Fragments arranged by size: 2229 2207 1545 158 146 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6382 PciI A'CATG_T Cuts at: 3578 5281 3578 Size: 1703 4679 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6382 PfoI T'CCnGG_A Cuts at: 2050 2554 2641 2050 Size: 504 87 5791 Fragments arranged by size: 5791 504 87 PmeI GTTT'AAAC Cuts at: 3686 3686 Size: 6382 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2103 2135 4451 4483 5964 5996 2103 Size: 32 2316 32 1481 32 2489 Fragments arranged by size: 2489 2316 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6382 PsiI TTA'TAA Cuts at: 3952 3952 Size: 6382 PstI C_TGCA'G Cuts at: 830 3734 830 Size: 2904 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6382 SalI G'TCGA_C Cuts at: 1936 3724 1936 Size: 1788 4594 SbfI CC_TGCA'GG Cuts at: 3734 3734 Size: 6382 ScaI AGT'ACT Cuts at: 4630 6342 4630 Size: 1712 4670 SfcI C'TryA_G Cuts at: 826 1045 2208 3318 3730 4865 5546 5737 826 Size: 219 1163 1110 412 1135 681 191 1471 Fragments arranged by size: 1471 1163 1135 1110 681 412 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6382 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6382 SmaI CCC'GGG Cuts at: 1872 3709 1872 Size: 1837 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2251 3068 3075 3600 4486 5387 Size: 197 926 308 817 7 525 886 901 Cuts at: 5387 5649 820 Size: 262 1553 Fragments arranged by size: 1553 926 901 886 817 525 308 262 197 7 SnaBI TAC'GTA Cuts at: 493 4173 493 Size: 3680 2702 SpeI A'CTAG_T Cuts at: 152 2804 152 Size: 2652 3730 SphI G_CATG'C Cuts at: 1132 3740 1132 Size: 2608 3774 SspI AAT'ATT Cuts at: 5 52 3106 3526 4306 5 Size: 47 3054 420 780 2081 Fragments arranged by size: 3054 2081 780 420 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6382 StyI C'CwwG_G Cuts at: 513 1065 2004 2342 3135 3187 3821 513 Size: 552 939 338 793 52 634 3074 Fragments arranged by size: 3074 939 793 634 552 338 52 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2842 4409 4426 4579 4764 5209 325 Size: 1255 1262 1567 17 153 185 445 1498 Fragments arranged by size: 1567 1498 1262 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4628 6340 96 Size: 274 80 33 51 1443 2651 1712 138 Fragments arranged by size: 2651 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6342 6374 6342 Size: 32 6350 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6382 XbaI T'CTAG_A Cuts at: 3718 3718 Size: 6382 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2988 2988 Size: 6382 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6382 XmaI C'CCGG_G Cuts at: 1870 3707 1870 Size: 1837 4545 XmnI GAAnn'nnTTC Cuts at: 2229 2923 4511 2229 Size: 694 1588 4100 Fragments arranged by size: 4100 1588 694 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4190 276 Size: 53 83 186 936 2656 2468 Fragments arranged by size: 2656 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI