Restriction map for
pFN21AE9612
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaII AviII
AvrII BalI BanII BbvCI BcgI
BciVI BfaI BfrI BglI BglII
BlnI BlpI BmyI BpmI Bpu1102I
BsaBI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstZ17I Bsu36I
CelII Cfr10I Cfr13I ClaI CpoI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoRI EcoT22I FseI FspI HgaI
HinfI KpnI KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MunI MvnI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI Sau96I
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI TaqI TfiI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccI 167 GTCTAC GT^[AC][GT]AC
AcyI 34 GGCGCC G[GA]^CG[CT]C
AlwNI 172 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 560 CCCGGG C^[CT]CG[GA]G
BamHI 273 GGATCC G^GATCC
BanI 34 GGCGCC G^G[CT][GA]CC
BbeI 34 GGCGCC GGCGC^C
BbrPI 46 CACGTG CAC^GTG
BclI 339 TGATCA T^GATCA
BsaAI 46 CACGTG [CT]AC^GT[GA]
BsaHI 34 GGCGCC G[GA]^CG[CT]C
BsmFI 23 GGGAC GGGAC(10/14)
BsoBI 560 CCCGGG C^[CT]CG[GA]G
BsrDI 719 CATTGC GCAATG(2/0)
BsrI 117 ACTGG ACTGG(1/-1)
BstEII 589 GGTGACC G^GT[ACGT]ACC
BstF5I 657 CATCC GGATG(2/0)
BstPI 589 GGTGACC G^GT[ACGT]ACC
BstXI 263 CCACACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 273 GGATCC [GA]^GATC[CT]
Cac8I 7 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 35 GCGC GCG^C
DraI 69 TTTAAA TTT^AAA
DsaI 636 CCATGG C^C[GA][CT]GG
EaeI 2 TGGCCG [CT]^GGCC[GA]
EarI 613 GAAGAG CTCTTC(1/4)
EcoNI 300 CCTCATAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 589 GGTGACC G^GT[ACGT]ACC
EcoRV 645 GATATC GAT^ATC
EcoT14I 636 CCATGG C^C[AT][AT]GG
FauI 20 GCGGG CCCGC(4/6)
FbaI 339 TGATCA T^GATCA
FokI 657 CATCC GGATG(9/13)
HaeII 34 GGCGCC [GA]GCGC^[CT]
HaeIII 3 GGCC GG^CC
HhaI 35 GCGC GCG^C
Hin1I 34 GGCGCC G[GA]^CG[CT]C
HinP1I 35 GCGC G^CGC
HpaI 121 GTTAAC GTT^AAC
HphI 589 GGTGA GGTGA(8/7)
KasI 34 GGCGCC G^GCGCC
Ksp632I 613 GAAGAG CTCTTC(1/4)
MflI 273 GGATCC [GA]^GATC[CT]
NarI 34 GGCGCC GG^CGCC
NcoI 636 CCATGG C^CATGG
PmaCI 46 CACGTG CAC^GTG
PmlI 46 CACGTG CAC^GTG
PstI 839 CTGCAG CTGCA^G
PvuII 175 CAGCTG CAG^CTG
SfoI 34 GGCGCC GGC^GCC
SmaI 560 CCCGGG CCC^GGG
SmlI 400 CTTGAG C^T[CT][GA]AG
StyI 636 CCATGG C^C[AT][AT]GG
TspRI 603 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 273 GGATCC [GA]^GATC[CT]
XmaCI 560 CCCGGG C^CCGGG
XmaI 560 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AciI 5 CCGC CCGC(-3/-1)
20 GCGG
AfaI 471 GTAC GT^AC
565 GTAC
AflIII 47 ACGTGT A^C[GA][CT]GT
359 ACGTGT
AlwI 274 GATCC GGATC(4/5)
273 GGATC
BsaJI 560 CCCGGG C^C[ACGT][ACGT]GG
636 CCATGG
BstNI 54 CCTGG CC^[AT]GG
394 CCTGG
DdeI 788 CTAAG C^T[ACGT]AG
845 CTCAG
DpnII 274 GATC ^GATC
340 GATC
EcoRII 54 CCTGG ^CC[AT]GG
394 CCTGG
HapII 39 CCGG C^CGG
561 CCGG
HindIII 596 AAGCTT A^AGCTT
697 AAGCTT
HpaII 39 CCGG C^CGG
561 CCGG
MaeII 47 ACGT A^CGT
359 ACGT
MboI 274 GATC ^GATC
340 GATC
MslI 264 CACACCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
488 CATTAAAGTG
MspA1I 5 CCGCTG C[AC]G^C[GT]G
175 CAGCTG
MspI 39 CCGG C^CGG
561 CCGG
MvaI 54 CCTGG CC^[AT]GG
394 CCTGG
MwoI 11 GCGTGGACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
844 GCTCAGAAGGC
NdeII 274 GATC ^GATC
340 GATC
NlaIII 418 CATG CATG^
637 CATG
RsaI 471 GTAC GT^AC
565 GTAC
Sau3AI 274 GATC ^GATC
340 GATC
SfaNI 76 GATGC GCATC(5/9)
374 GCATC
SspI 65 AATATT AAT^ATT
461 AATATT
Tsp45I 421 GTGAC ^GT[GC]AC
590 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AluI BbsI BbvI BcnI BpuAI
BsiYI BslI BssKI Fnu4HI HincII
HindII ItaI MaeIII MboII MnlI
MseI NciI NlaIV ScrFI SfcI
Tru9I TseI Tsp509I