Restriction map for
pFN21AE5450
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BpmI BsaBI BsaHI BsaI
BsaJI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DraII DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HindIII HpaI
KasI KpnI Ksp632I KspI MamI
MfeI MluI MroI MslI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TfiI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcsI 152 AAATTC [GA]^AATT[CT]
ApoI 152 AAATTC [GA]^AATT[CT]
AspHI 359 GTGCTC G[AT]GC[AT]^C
AvaII 711 GGACC G^G[AT]CC
BlpI 199 GCTCAGC GC^T[ACGT]AGC
BmyI 359 GTGCTC G[AGT]GC[ACT]^C
Bpu1102I 199 GCTCAGC GC^T[ACGT]AGC
BsaAI 511 TACGTG [CT]AC^GT[GA]
BseRI 385 CTCCTC GAGGAG(10/8)
BsiHKAI 359 GTGCTC G[AT]GC[AT]^C
BsiYI 780 CCTCCTTCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 780 CCTCCTTCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 797 GTCCC GGGAC(10/14)
Bsp1286I 359 GTGCTC G[AGT]GC[ACT]^C
BspMI 254 ACCTGC ACCTGC(4/8)
BsrDI 142 GCAATG GCAATG(2/0)
BssKI 708 CCTGG ^CC[ACGT]GG
BstNI 708 CCTGG CC^[AT]GG
CelII 199 GCTCAGC GC^T[ACGT]AGC
DraI 684 TTTAAA TTT^AAA
EcoRII 708 CCTGG ^CC[AT]GG
HapII 754 CCGG C^CGG
HincII 522 GTCAAC GT[CT]^[GA]AC
HindII 522 GTCAAC GT[CT]^[GA]AC
HinfI 336 GAGTC G^A[ACGT]TC
HpaII 754 CCGG C^CGG
HphI 237 GGTGA GGTGA(8/7)
MspA1I 259 CAGCTG C[AC]G^C[GT]G
MspI 754 CCGG C^CGG
MvaI 708 CCTGG CC^[AT]GG
NheI 204 GCTAGC G^CTAGC
NlaIV 1005 GGCCCC GG[ACGT]^[ACGT]CC
PleI 336 GAGTC GAGTC(4/5)
PstI 256 CTGCAG CTGCA^G
PvuII 259 CAGCTG CAG^CTG
ScrFI 708 CCTGG CC^[ACGT]GG
SfaNI 544 GATGC GCATC(5/9)
SmlI 312 CTTGAG C^T[CT][GA]AG
TaqI 450 TCGA T^CGA
TthHB8I 450 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 164 GTAC GT^AC
510 GTAC
Asp700I 442 GAATGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
814 GAAGCCTTTC
BalI 74 TGGCCA TGG^CCA
842 TGGCCA
BbsI 317 GAAGAC GAAGAC(2/6)
631 GAAGAC
BfaI 205 CTAG C^TAG
694 CTAG
BpuAI 317 GAAGAC GAAGAC(2/6)
631 GAAGAC
BsmAI 675 GAGAC GTCTC(1/5)
989 GAGAC
BspHI 731 TCATGA T^CATGA
770 TCATGA
BsrI 807 CCAGT ACTGG(1/-1)
845 CCAGT
BstYI 875 AGATCC [GA]^GATC[CT]
983 AGATCC
Cfr13I 711 GGACC G^G[ACGT]CC
1005 GGCCC
EaeI 74 TGGCCA [CT]^GGCC[GA]
842 TGGCCA
MaeI 205 CTAG C^TAG
694 CTAG
MaeII 420 ACGT A^CGT
512 ACGT
MflI 875 AGATCC [GA]^GATC[CT]
983 AGATCC
MluNI 74 TGGCCA TGG^CCA
842 TGGCCA
MscI 74 TGGCCA TGG^CCA
842 TGGCCA
RcaI 731 TCATGA T^CATGA
770 TCATGA
RsaI 164 GTAC GT^AC
510 GTAC
Sau96I 711 GGACC G^G[ACGT]CC
1005 GGCCC
SfcI 219 CTACAG C^T[GA][CT]AG
256 CTGCAG
XhoII 875 AGATCC [GA]^GATC[CT]
983 AGATCC
XmnI 442 GAATGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
814 GAAGCCTTTC
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# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AluI AlwI BbvI BstF5I Cac8I
DdeI DpnII Fnu4HI FokI HaeIII
ItaI MaeIII MboI MboII MnlI
MseI MwoI NdeII NlaIII Sau3AI
Tru9I TseI Tsp45I Tsp509I TspRI